Prevalence of intestinal parasites with molecular detection and identification of Giardia duodenalis in fecal samples of mammals, birds and zookeepers at Beni-Suef Zoo, Egypt

2021 ◽  
Author(s):  
Asmaa Alaa Kamel ◽  
Gihan K. Abdel-Latef
Keyword(s):  
Molecular Detection ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Fecal Samples ◽  
Detection And Identification

scholarly journals Canine Fecal Contamination in a Metropolitan Area (Milan, North-Western Italy): Prevalence of Intestinal Parasites and Evaluation of Health Risks

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sergio Aurelio Zanzani ◽  
Anna Rita Di Cerbo ◽  
Alessia Libera Gazzonis ◽  
Marco Genchi ◽  
Laura Rinaldi ◽  
...  
Keyword(s):  
Metropolitan Area ◽  
Intestinal Parasites ◽  
Fecal Contamination ◽  
Giardia Duodenalis ◽  
Strongyloides Stercoralis ◽  
Toxocara Canis ◽  
Geographical Information ◽  
Fecal Samples ◽  
Zoonotic Parasites ◽  
North Western

Intestinal parasites of dogs represent a serious threat to human health due to their zoonotic potential. Thus, metropolitan areas presenting high concentrations of pets and urban fecal contamination on public areas are at sanitary risk. Major aim of this survey was to determine prevalence of zoonotic parasites in dog fecal samples collected from public soil of Milan (north-western Italy). Differences in parasites prevalence distribution were explored by a geographical information system- (GIS-) based approach, and risk factors (human density, sizes of green parks, and dog areas) were considered. The metropolitan area was divided into 157 rectangular subareas and sampling was performed following a 1-kilometer straight transect. A total of 463 fecal samples were analyzed using centrifugation-flotation technique and ELISA to detectGiardiaandCryptosporidiumcoproantigens. A widespread fecal contamination of soil was highlighted, being fecal samples found in 86.8% of the subareas considered. The overall prevalence of intestinal parasites was 16.63%. Zoonotic parasites were found, such asTrichuris vulpis(3.67%),Toxocara canis(1.72%),Strongyloides stercoralis(0.86%), Ancylostomatidae (0.43%), andDipylidium caninum(0.43%).Giardia duodenaliswas the most prevalent zoonotic protozoa (11.06%), followed byCryptosporidium(1.10%). Faeces from subareas characterized by broad green areas showed to be particularly prone to infection.

Molecular studies on pig cryptosporidiosis in Poland

2014 ◽  
Vol 17 (4) ◽  
pp. 577-582 ◽  
Author(s):  
A. Rzeżutka ◽  
A. Kaupke ◽  
I. Kozyra ◽  
Z. Pejsak
Keyword(s):  
Intestinal Parasites ◽  
Cryptosporidium Species ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Nucleotide Sequence Analysis ◽  
Fecal Samples ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Pig Farms ◽  
Asymptomatic Infections

AbstractCryptosporidium intestinal parasites have been detected in farmed pigs worldwide. Infections are usually asymptomatic with a low number of oocysts shed in pig feces. This makes the recognition of infection difficult or unsuccessful when microscopic methods are used. The aim of this study was molecular identification of Cryptosporidium species in pig herds raised in Poland with regard to the occurrence of zoonotic species. In total, 166 pig fecal samples were tested. The examined pigs were aged 1 to 20 weeks. Overall, 39 pig farms were monitored for parasite presence. The detection and identification of Cryptosporidium DNA was performed on the basis of PCR-RFLP and nucleotide sequence analysis of the amplified 18 SSU rRNA and COWP gene fragments. Infected animals were housed in 21 (53.8%) of the pig farms monitored. The presence of Cryptosporidum was confirmed in 46 (27.7%) samples of pig feces. Among positive fecal samples, 34 (29.3%) were collected from healthy animals, and 12 (24%) from diarrheic pigs. Most infected animals (42.1%) were 2 to 3 months old. The following parasite species were detected: C. scrofarum, C. suis and C. parvum. Indeed, asymptomatic infections caused by C. scrofarum were observed in the majority of the herds. Mixed infections caused by C. suis and C. scrofarum were not common; however, they were observed in 8.6% of the positive animals. C. parvum DNA was found only in one sample collected from a diarrheic pig. The application of molecular diagnostic tools allowed for detection and identification of Cryptosporidium species in pigs. The sporadic findings of C. parvum are subsequent evidence for the contribution of pigs in the transmission of cryptosporidiosis from animals to humans.

Molecular detection of Cryptosporidium spp. and the occurrence of intestinal parasites in fecal samples of naturally infected dogs and cats

2018 ◽  
Vol 117 (9) ◽  
pp. 3033-3038 ◽  
Author(s):  
Marta Elena Machado Alves ◽  
Felippe Danyel Cardoso Martins ◽  
Patrícia Bräunig ◽  
Felipe Lamberti Pivoto ◽  
Luís Antonio Sangioni ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Intestinal Parasites ◽  
Fecal Samples ◽  
Cryptosporidium Spp

scholarly journals Giardia Duodenalis e Desnutrição Crónica em Crianças Menores de Cinco Anos de uma Região Rural da Guiné-Bissau

2013 ◽  
Vol 26 (6) ◽  
pp. 721
Author(s):  
Sónia Centeno-Lima ◽  
Vítor Rosado-Marques ◽  
Filipa Ferreira ◽  
Ruben Rodrigues ◽  
Benjamim Indeque ◽  
...  
Keyword(s):  
Rural Community ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Microscopic Analysis ◽  
Z Score ◽  
Chronic Malnutrition ◽  
Under Five ◽  
Detection And Identification ◽  
Geographical Regions ◽  
Height For Age

Introduction: Malnutrition and infections by intestinal parasites such as Giardia duodenalis coexist in the same geographical regions, reaching the highest prevalence in developing countries. The cycle of malnutrition and infection implies that both conditions can aggravate each other and compromise the growth and development of children with special relevance for under-five. The aim of this study was to investigate the association between chronic malnutrition and infection by G. duodenalis in children under five in a rural community in Guinea-Bissau.Material and Methods: A case-control study that included 109 children aged 0 to 59 months of a rural community in Guinea-Bissau was conducted. The anthropometric assessment of children in the study identified 31 cases of chronic malnutrition (z-score height for age < -2) and 78 controls (z-score height for age ≥ -2). Microscopic examination of stools was performed for detection and identification of G. duodenalis and other parasites.Results: The microscopic analysis of stool samples revealed G. duodenalis infection in 29.0% (9/31) of cases and 35.9% (28/78) of controls. No association between the infection with G. duodenalis and chronic malnutrition in children under study could be established.Discussion and Conclusion: The results reinforce the interest in designing further studies exploring this association in differentregions and epidemiological settings, while direct to the importance of the criteria for malnutrition definition which influences the subsequent analysis.

Comparative analysis of routine parasitological methods for recovery of cysts, molecular detection, and genotyping of Giardia duodenalis

2021 ◽  
Author(s):  
Renata Coltro Bezagio ◽  
Cristiane Maria Colli ◽  
Liara Izabela Lopes Romera ◽  
Caroline Rodrigues de Almeida ◽  
Érika Cristina Ferreira ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Molecular Detection ◽  
Giardia Duodenalis

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

Protozoan infection in archaeological material

1970 ◽  
Vol 80 (1) ◽  
Author(s):  
Marcelo Luiz Carvalho Gonçalves ◽  
Cassius Schnell ◽  
Luciana Sianto ◽  
Francoise Bouchet ◽  
Mathieu Le Bailly ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
19Th Century ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Human Feces ◽  
Archaeological Material ◽  
The Past ◽  
Protozoan Infection ◽  
Protein Molecules ◽  
The 19Th Century

The identification of parasites in ancient human feces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment. It was possible to detected antigen of Entamoeba histolytica and Giardia duodenalis in historic and prehistoric human fecal remains using two enzyme immunoassay (ELISA) kits with monoclonal antibodies specific for E. histolytica and G. duodenalis, respectively. Specimens of desiccated feces and ancient latrine sediment from the New and the Old World were examined. The ELISA detected E. histolytica antigen in samples from Argentina, USA, France, Belgium, and Switzerland, dated to about 5300 years BP to the 19th Century AD. G. duodenalis antigen was detected in samples from USA, Belgium, and Germany, dated to about 1200 AD, 1600 AD, and 1700 AD. The detection of protozoan antigen using immunoassays is a reliable tool for the study of intestinal parasites in the past.

scholarly journals Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

2016 ◽  
Vol 14 (1) ◽  
pp. 63-68 ◽  
Author(s):  
MM Akter ◽  
S Majumder ◽  
KH MNH Nazir ◽  
M Rahman
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Detection ◽  
Chain Reaction ◽  
Specific Primers ◽  
Fecal Samples ◽  
E Coli ◽  
Uremic Syndrome ◽  
Polymerase Chain ◽  
Positive Isolate

Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.J. Bangladesh Agril. Univ. 14(1): 63-68, June 2016

scholarly journals Giardia duodenalis: genotypic comparison between a human and a canine isolates

2011 ◽  
Vol 44 (4) ◽  
pp. 508-510 ◽  
Author(s):  
Karina Braga Gomes ◽  
Ana Paula Fernandes ◽  
Aline Menezes ◽  
Ronaldo Amorim Júnior ◽  
Edward Félix Silva ◽  
...  
Keyword(s):  
Giardia Duodenalis ◽  
Zoonotic Disease ◽  
Specific Region ◽  
Genetic Identity ◽  
Fecal Samples ◽  
Belo Horizonte

INTRODUCTION: Evidence suggests that giardiasis is a zoonotic disease. The present work aimed to evaluate the genetic identity of Giardia duodenalis isolated from human and dog fecal samples from Belo Horizonte. METHODS: Human and dog fecal samples were cultured for isolation of G. duodenalis. To determine the genotype of the isolates, primers that amplify a specific region in rRNA of the protozoan were used. RESULTS: Two G. duodenalis isolates were obtained, which belong to the subgroup A genotype. CONCLUSIONS: These findings suggest that the transmission of giardiasis follows a zoonotic pattern.

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