Molecular Profile and Clinico-pathological Characteristics of Breast Cancer in Central India: First Investigative Report

2022 ◽  
Author(s):  
Aaryan Anand ◽  
Arpan Mishra ◽  
Harikrishna Damde ◽  
Arjun Saxena ◽  
Sanjay Kumar Yadav ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Central India ◽  
Molecular Profile ◽  
Pathological Characteristics ◽  
Investigative Report

scholarly journals Polyphenol-Enriched Blueberry Preparation Controls Breast Cancer Stem Cells by Targeting FOXO1 and miR-145

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4330
Author(s):  
Jean-François Mallet ◽  
Roghayeh Shahbazi ◽  
Nawal Alsadi ◽  
Chantal Matar
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Mirna Expression ◽  
Epigenetic Mechanism ◽  
Breast Cancer Cell Lines ◽  
Relative Expression ◽  
Target Proteins ◽  
Pathological Characteristics

Scientific evidence supports the early deregulation of epigenetic profiles during breast carcinogenesis. Research shows that cellular transformation, carcinogenesis, and stemness maintenance are regulated by epigenetic-specific changes that involve microRNAs (miRNAs). Dietary bioactive compounds such as blueberry polyphenols may modulate susceptibility to breast cancer by the modulation of CSC survival and self-renewal pathways through the epigenetic mechanism, including the regulation of miRNA expression. Therefore, the current study aimed to assay the effect of polyphenol enriched blueberry preparation (PEBP) or non-fermented blueberry juice (NBJ) on the modulation of miRNA signature and the target proteins associated with different clinical-pathological characteristics of breast cancer such as stemness, invasion, and chemoresistance using breast cancer cell lines. To this end, 4T1 and MB-MDM-231 cell lines were exposed to NBJ or PEBP for 24 h. miRNA profiling was performed in breast cancer cell cultures, and RT-qPCR was undertaken to assay the expression of target miRNA. The expression of target proteins was examined by Western blotting. Profiling of miRNA revealed that several miRNAs associated with different clinical-pathological characteristics were differentially expressed in cells treated with PEBP. The validation study showed significant downregulation of oncogenic miR-210 expression in both 4T1 and MDA-MB-231 cells exposed to PEBP. In addition, expression of tumor suppressor miR-145 was significantly increased in both cell lines treated with PEBP. Western blot analysis showed a significant increase in the relative expression of FOXO1 in 4T1 and MDA-MB-231 cells exposed to PEBP and in MDA-MB-231 cells exposed to NBJ. Furthermore, a significant decrease was observed in the relative expression of N-RAS in 4T1 and MDA-MB-231 cells exposed to PEBP and in MDA-MB-231 cells exposed to NBJ. Our data indicate a potential chemoprevention role of PEBP through the modulation of miRNA expression, particularly miR-210 and miR-145, and protection against breast cancer development and progression. Thus, PEBP may represent a source for novel chemopreventative agents against breast cancer.

scholarly journals Transcriptome profiles of stem-like cells from primary breast cancers allow identification of ITGA7 as a predictive marker of chemotherapy response

2021 ◽  
Author(s):  
Noha Gwili ◽  
Stacey J. Jones ◽  
Waleed Al Amri ◽  
Ian M. Carr ◽  
Sarah Harris ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Predictive Marker ◽  
Therapy Resistance ◽  
Differentially Expressed ◽  
Molecular Profile ◽  
Chemotherapy Response ◽  
Breast Cancers

Abstract Background Breast cancer stem cells (BCSCs) are drivers of therapy-resistance, therefore are responsible for poor survival. Molecular signatures of BCSCs from primary cancers remain undefined. Here, we identify the consistent transcriptome of primary BCSCs shared across breast cancer subtypes, and we examine the clinical relevance of ITGA7, one of the genes differentially expressed in BCSCs. Methods Primary BCSCs were assessed using immunohistochemistry and fluorescently labelled using Aldefluor (n = 17). Transcriptomes of fluorescently sorted BCSCs and matched non-stem cancer cells were determined using RNA-seq (n = 6). ITGA7 expression was examined in breast cancers using immunohistochemistry (n = 305), and its functional role was tested using siRNA in breast cancer cells. Results Proportions of BCSCs varied from 0 to 9.4%. 38 genes were significantly differentially expressed in BCSCs; genes were enriched for functions in vessel morphogenesis, motility, and metabolism. ITGA7 was found to be significantly downregulated in BCSCs, and low expression significantly correlated with reduced survival in patients treated with chemotherapy, and with chemoresistance in breast cancer cells in vitro. Conclusions This study is the first to define the molecular profile of BCSCs from a range of primary breast cancers. ITGA7 acts as a predictive marker for chemotherapy response, in accordance with its downregulation in BCSCs.

Comparative study of the molecular profile of the inflammatory and non-inflammatory breast cancer in Tunisia

2008 ◽  
Vol 6 (9) ◽  
pp. 169-170
Author(s):  
R. Marrakchi ◽  
I. Khadimallah ◽  
K. Bougatef ◽  
S. Ouerhani ◽  
Y. Messai ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Comparative Study ◽  
Inflammatory Breast Cancer ◽  
Molecular Profile

scholarly journals Efficacy of angiotensin-receptor blockers on demographic and clinico-pathological characteristics of breast cancer

The Breast ◽  
2012 ◽  
Vol 21 (3) ◽  
pp. 419-420 ◽  
Author(s):  
Mehmet A.N. Şendur ◽  
Sercan Aksoy ◽  
Sebnem Yaman ◽  
Nuriye Y. Ozdemir ◽  
Nurullah Zengin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Angiotensin Receptor Blockers ◽  
Angiotensin Receptor ◽  
Pathological Characteristics ◽  
Receptor Blockers

Interval breast carcinoma: A comparison of clinical-pathological characteristics between symptomatic and screen-detected breast cancer

2014 ◽  
Vol 40 (5) ◽  
pp. 646-647
Author(s):  
B. Meshkat ◽  
R.S. Prichard ◽  
Z. Al-Hili ◽  
G.B. Bass ◽  
C. Quinn ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Pathological Characteristics

scholarly journals Clinical and pathological characteristics of breast cancer patients with history of tonsillectomy

The Breast ◽  
2010 ◽  
Vol 19 (2) ◽  
pp. 152 ◽  
Author(s):  
Metin Isik ◽  
Sercan Aksoy ◽  
Didem S. Dede ◽  
Omer Dizdar ◽  
Atilla Kara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Breast Cancer Patients ◽  
Pathological Characteristics ◽  
History Of

scholarly journals A retrospective study of colorectal carcinoma in central India

2017 ◽  
Vol 4 (3) ◽  
pp. 977
Author(s):  
Suresh Clement H. ◽  
S. Madhu Babu ◽  
Samir Ahmad ◽  
Harsha Omkar M.
Keyword(s):  
Breast Cancer ◽  
Central India ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
Age Group ◽  
Cross Sectional ◽  
Outer Quadrant ◽  
The World ◽  
Menstruating Women ◽  
Epidemiological Profile

Background: Inner lining of lobules or ducts of milk of breast tissue is the origin of the breast cancer. Among females, it constitutes 10.4% of cancer cases incidence all over the world. It thus is the fifth leading cause of mortality all over the world. As known, it is more common in females than males. The objective of this study was to study the clinical and epidemiological profile of breast cancer patients.Methods: A hospital based cross sectional study was carried out for a period of two and half years at M. G. M. Hospital, Warangal. Both males and females of 30 years and above were included in the study. Total number of carcinoma cases admitted during 2005 to 2007 for a period of two and half years were 1,428. Total number of carcinoma breast cases admitted during 2005 to 2007 for a period of two and half years were 242.Results: Maximum cases i.e. 44.6% belonged to stage III of breast cancer. Maximum cases were seen among Hindus i.e. 76.4%. As expected, only one case was reported by males which constituted only 0.42%. Highest number of cases i.e. 99 (40.9%) were reported during the age group of 40-50 years. Multi para women constituted more number of cases in 78.8%. Maximum number of cases of breast cancer i.e. 41.6% occurred among menstruating women. Most commonly affected quadrant was upper outer in 55.1% of cases. The most common type was Schirrous carcinoma in 39.6% of cases.Conclusions: Upper outer quadrant was most commonly affected. This indicates that the women in the reproductive age group should be directed to examine their breast daily with specific attention to upper outer quadrant.

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