Altered expression patterns of clock gene mRNAs and clock proteins in human skin tumors

Psoriasis is a systemic inflammatory skin disorder that can be associated with sleep disturbance and negatively influence the daily rhythm. The link between the pathomechanism of psoriasis and the circadian rhythm has been suggested by several previous studies. However, there are insufficient data on altered clock mechanisms in psoriasis to prove these theories. Therefore, we investigated the expression of the core clock genes in human psoriatic lesional and non-lesional skin and in human adult low calcium temperature (HaCaT) keratinocytes after stimulation with pro-inflammatory cytokines. Furthermore, we examined the clock proteins in skin biopsies from psoriatic patients by immunohistochemistry. We found that the clock gene transcripts were elevated in psoriatic lesions, especially in non-lesional psoriatic areas, except for rev-erbα, which was consistently downregulated in the psoriatic samples. In addition, the REV-ERBα protein showed a different epidermal distribution in non-lesional skin than in healthy skin. In cytokine-treated HaCaT cells, changes in the amplitude of the bmal1, cry1, rev-erbα and per1 mRNA oscillation were observed, especially after TNFα stimulation. In conclusion, in our study a perturbation of clock gene transcripts was observed in uninvolved and lesional psoriatic areas compared to healthy skin. These alterations may serve as therapeutic targets and facilitate the development of chronotherapeutic strategies in the future.

Download Full-text

Sodium Iodate-Induced Mouse Model of Age-Related Macular Degeneration Displayed Altered Expression Patterns of Sumoylation Enzymes E1, E2 and E3

Current Molecular Medicine ◽

10.2174/1566524019666190112101147 ◽

2019 ◽

Vol 18 (8) ◽

pp. 550-555 ◽

Cited By ~ 3

Author(s):

Qian Nie ◽

Xiaodong Gong ◽

Lili Gong ◽

Lan Zhang ◽

Xiangcheng Tang ◽

...

Keyword(s):

Mouse Model ◽

Macular Degeneration ◽

Expression Patterns ◽

Age Related Macular Degeneration ◽

Sodium Iodate ◽

Altered Expression ◽

Age Related

Download Full-text

Interstitial Collagenase and the ED-B Oncofetal Domain of Fibronectin Are Markers of Angiogenesis in Human Skin Tumors

Cancer Detection and Prevention ◽

10.1046/j.1525-1500.1998.00061.x ◽

1998 ◽

Vol 22 (5) ◽

pp. 438-444 ◽

Cited By ~ 14

Author(s):

Tatiana V. Karelina ◽

Arthur Z. Eisen

Keyword(s):

Human Skin ◽

Skin Tumors ◽

Interstitial Collagenase

Download Full-text

Ras, TrkB, and ShcA Protein Expression Patterns in Pediatric Brain Tumors

Journal of Clinical Medicine ◽

10.3390/jcm10102219 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2219

Author(s):

Monika Prill ◽

Agnieszka Karkucinska-Wieckowska ◽

Magdalena Lebiedzinska-Arciszewska ◽

Giampaolo Morciano ◽

Agata Charzynska ◽

...

Keyword(s):

Brain Tumors ◽

Expression Patterns ◽

Pediatric Brain Tumors ◽

Astrocytic Tumors ◽

Embryonal Tumors ◽

Altered Expression ◽

Malignancy Grade ◽

Oligodendroglial Tumors ◽

Different Types ◽

Pediatric Brain

Numerous papers have reported altered expression patterns of Ras and/or ShcA proteins in different types of cancers. Their level can be potentially associated with oncogenic processes. We analyzed samples of pediatric brain tumors reflecting different groups such as choroid plexus tumors, diffuse astrocytic and oligodendroglial tumors, embryonal tumors, ependymal tumors, and other astrocytic tumors as well as tumor malignancy grade, in order to characterize the expression profile of Ras, TrkB, and three isoforms of ShcA, namely, p66Shc, p52Shc, and p46Shc proteins. The main aim of our study was to evaluate the potential correlation between the type of pediatric brain tumors, tumor malignancy grade, and the expression patterns of the investigated proteins.

Download Full-text

Betulinic Acid Affects the Energy-Related Proteomic Profiling in Pancreatic Ductal Adenocarcinoma Cells

Molecules ◽

10.3390/molecules26092482 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2482

Author(s):

Ching-Feng Chiu ◽

Hsin-Yi Chang ◽

Chun-Yine Huang ◽

Chen-Zou Mau ◽

Tzu-Ting Kuo ◽

...

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

Betulinic Acid ◽

Expression Patterns ◽

Apolipoprotein A1 ◽

Ductal Adenocarcinoma ◽

Therapeutic Window ◽

Pdac Cell ◽

Cell Growth And Migration ◽

Altered Expression ◽

Clinical Prognosis

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with a 5-year survival rate of <8%. Therefore, finding new treatment strategies against PDAC cells is an imperative issue. Betulinic acid (BA), a plant-derived natural compound, has shown great potential to combat cancer owing to its versatile physiological functions. In this study, we observed the impacts of BA on the cell viability and migratory ability of PDAC cell lines, and screened differentially expressed proteins (DEPs) by an LC-MS/MS-based proteomics analysis. Our results showed that BA significantly inhibited the viability and migratory ability of PDAC cells under a relatively low dosage without affecting normal pancreatic cells. Moreover, a functional analysis revealed that BA-induced downregulation of protein clusters that participate in mitochondrial complex 1 activity and oxidative phosphorylation, which was related to decreased expressions of RNA polymerase mitochondrial (POLRMT) and translational activator of cytochrome c oxidase (TACO1), suggesting that the influence on mitochondrial function explains the effect of BA on PDAC cell growth and migration. In addition, BA also dramatically increased Apolipoprotein A1 (APOA1) expression and decreased NLR family CARD domain-containing protein 4 (NLRC4) expression, which may be involved in the dampening of PDAC migration. Notably, altered expression patterns of APOA1 and NLRC4 indicated a favorable clinical prognosis of PDAC. Based on these findings, we identified potential proteins and pathways regulated by BA from a proteomics perspective, which provides a therapeutic window for PDAC.

Download Full-text

Macromolecule biosynthesis: a key function of sleep

Physiological Genomics ◽

10.1152/physiolgenomics.00275.2006 ◽

2007 ◽

Vol 31 (3) ◽

pp. 441-457 ◽

Cited By ~ 213

Author(s):

Miroslaw Mackiewicz ◽

Keith R. Shockley ◽

Micah A. Romer ◽

Raymond J. Galante ◽

John E. Zimmerman ◽

...

Keyword(s):

Gene Expression ◽

Cerebral Cortex ◽

Sleep Deprivation ◽

Expression Patterns ◽

State Level ◽

Altered Expression ◽

Mouse Cerebral Cortex ◽

Genes Encoding ◽

Function Of Sleep ◽

Cellular Components

The function(s) of sleep remains a major unanswered question in biology. We assessed changes in gene expression in the mouse cerebral cortex and hypothalamus following different durations of sleep and periods of sleep deprivation. There were significant differences in gene expression between behavioral states; we identified 3,988 genes in the cerebral cortex and 823 genes in the hypothalamus with altered expression patterns between sleep and sleep deprivation. Changes in the steady-state level of transcripts for various genes are remarkably common during sleep, as 2,090 genes in the cerebral cortex and 409 genes in the hypothalamus were defined as sleep specific and changed (increased or decreased) their expression during sleep. The largest categories of overrepresented genes increasing expression with sleep were those involved in biosynthesis and transport. In both the cerebral cortex and hypothalamus, during sleep there was upregulation of multiple genes encoding various enzymes involved in cholesterol synthesis, as well as proteins for lipid transport. There was also upregulation during sleep of genes involved in synthesis of proteins, heme, and maintenance of vesicle pools, as well as antioxidant enzymes and genes encoding proteins of energy-regulating pathways. We postulate that during sleep there is a rebuilding of multiple key cellular components in preparation for subsequent wakefulness.

Download Full-text

Altered Expression of ESR1, ESR2, PELP1 and c-SRC Genes Is Associated with Ovarian Cancer Manifestation

International Journal of Molecular Sciences ◽

10.3390/ijms22126216 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6216

Author(s):

Monika Englert-Golon ◽

Mirosław Andrusiewicz ◽

Aleksandra Żbikowska ◽

Małgorzata Chmielewska ◽

Stefan Sajdak ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Biology ◽

Expression Patterns ◽

Control Group ◽

Tissue Samples ◽

Altered Expression ◽

Depth Study ◽

Tyrosine Protein Kinase ◽

Ovarian Tumorigenesis ◽

Protein Immunoreactivity

Ovarian cancer remains the leading cause of death due to gynecologic malignancy. Estrogen-related pathways genes, such as estrogen receptors (ESR1 and ESR2) and their coregulators, proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), and proto-oncogene tyrosine-protein kinase c-Src (SRC) are involved in ovarian cancer induction and development, still they require in-depth study. In our study, tissue samples were obtained from 52 females of Caucasian descent (control group without cancerous evidence (n = 27), including noncancerous benign changes (n = 15), and the ovarian carcinoma (n = 25)). Using quantitative analyses, we investigated ESRs, PELP1, and SRC mRNA expression association with ovarian tumorigenesis. Proteins’ presence and their location were determined by Western blot and immunohistochemistry. Results showed that PELP1 and SRC expression levels were found to differ in tissues of different sample types. The expression patterns were complex and differed in the case of ovarian cancer patients compared to controls. The most robust protein immunoreactivity was observed for PELP1 and the weakest for ESR1. The expression patterns of analyzed genes represent a potentially interesting target in ovarian cancer biology, especially PELP1. This study suggests that specific estrogen-mediated functions in the ovary and ovary-derived cancer might result from different local interactions of estrogen with their receptors and coregulators.

Download Full-text