Background:
The clinical application of Brinzolamide, a drug used in the treatment of
glaucoma is limited due its poor aqueous solubility. Microemulsion based ocular delivery can be an
effective means to improve its solubility and in turn the bioavailability.
Objective:
The main objective of the present work was optimization and characterization of Brinzolamide
loaded microemulsion for the treatment of glaucoma.
Method:
The solubility of Brinzolamide in various oils and surfactants was checked in order to
identify components of microemulsion. Pseudo-ternary phase diagram using Prosim software was
plotted to identify microemulsion existence area. D-optimal mixture design was used for optimization
of microemulsion. The optimized formulation consisted of Isopropyl myristate, Tween-80 and
Transcutol-P as surfactant and co-surfactant respectively, and water. The chosen critical responses
were droplet size, zeta potential, nepheloturbidimetric unit, and viscosity.
Results:
The selected optimal composition shows favorable features, such as droplet size (41.69
nm), Zeta potential (-9.496 mV), Viscosity (170.8 cps), Transparency (1.483 NTU) and pH (7.646)
that are suitable for ocular delivery. Moreover, a prolonged drug release (78.08 % within 7 hour)
was found in in-vitro experiments. By and large the formulation was found to be safe and nonirritant
as proven by the ocular irritation study.
Conclusion:
Our study illustrated potential of Brinzolamide loaded microemulsion for ocular delivery
for the treatment of glaucoma.
