Biological synthesis of ZnO nanoparticles using ethanolic extract of Satureja sahendica Bornm: its characterization and antimicrobial features

Nanoparticles are ultrafine structures with dimensions less than 100 nm. Nanoparticles have diverse applications. There are three important methods of fabrication of nanoparticles namely physical, chemical and biological methods. Physical method is a top down strategy for the fabrication of nanoparticles. It is energy intensive and time consuming. A chemical method is simple, but is expensive and requires expensive chemicals with high purity and also involves hazards of contaminations. Biological synthesis is very simple, cheap and environment friendly, requiring no expensive chemicals, temperature and is time saving. Plants and microorganisms are commonly used in this method. These are available everywhere. In the present work we synthesized Zinc Oxide (ZnO) nanoparticles by biological method using Aspargillus niger and zinc chloride (ZnCl2) as precursors. Biogenic synthesis of metallic nanoparticles by fungi is a safe and economical process because of formation of stable and small sized nanoparticles. Fungal biomass secretes proteins which act as reducing and stabilizing agents. The synthesized nanoparticles were characterized by XRD (X-Ray Diffraction), SEM (Scanning Electron Microscopy), UV-Vis (Ultraviolet, Visible) and EDX (Energy Dispersive X-Ray) techniques. Their size was in nm range and morphology of synthesized ZnO NPs was hexagonal. The ZnO nanoparticles are one of the most versatile materials and are used in cosmetics and in Bioenergy production, as a catalyst and as antibacterial material.

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Biofunctionalization and biological synthesis of the ZnO nanoparticles: The effect of Raphanus sativus (white radish) root extract on antimicrobial activity against MDR strain for wound healing applications

Inorganic Chemistry Communications ◽

10.1016/j.inoche.2018.12.014 ◽

2019 ◽

Vol 100 ◽

pp. 101-106 ◽

Cited By ~ 5

Author(s):

A.B.V. Kiran Kumar ◽

Edugulla Sre Saila ◽

Prachi Narang ◽

Mallelakari Aishwarya ◽

Rebha Raina ◽

...

Keyword(s):

Antimicrobial Activity ◽

Wound Healing ◽

Zno Nanoparticles ◽

Raphanus Sativus ◽

Biological Synthesis ◽

Root Extract ◽

Radish Root ◽

White Radish

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Biosynthesis of zinc oxide nanoparticles by using fruits extracts of Ananas Comosus and its antibacterial activity

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v15n2.1217 ◽

2019 ◽

Vol 15 (2) ◽

pp. 268-273 ◽

Cited By ~ 3

Author(s):

Raja Adibah Raja Ahmad ◽

Zawati Harun ◽

Mohd Hafiz Dzarfan Othman ◽

Hatijah Basri ◽

Muhamad Zaini Yunos ◽

...

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Metallic Nanoparticles ◽

Ananas Comosus ◽

Biological Synthesis ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

X Ray

Biosynthesis of metallic nanoparticles using plants, enzymes, and microorganism have been known as eco-friendly alternatives to conventional physical and chemical methods. Recently, the biological synthesis of nanoparticles has been a keen interest amongst researchers and scientist due to its simple technique, eco-friendliness, non-toxic, inexpensive and potential to perform in antibacterial activity. Thus, in this current work, the synthesis of zinc oxide (ZnO) nanoparticles using reduction agent from fruit extracts of Ananas Comosus is reported. The biosynthesized zinc oxide was characterized using Field Emission Scanning Electron Microscope (FESEM) with Energy Dispersive X-ray analysis (EDX), UV-Vis absorption spectroscopy and X-ray diffraction (XRD). The average size of the nanoparticles was found to be in the range of 30-57nm. The antibacterial activity of ZnO nanoparticles was carried out via agar diffusion method against pathogenic organisms. It is observed that the biosynthesized ZnO in the process has the efficient antibacterial activity. In conclusion, the green synthesis of zinc oxide nanoparticles using the fruit extract of Ananas Comosus is considered as a potential additive to substitute other metal oxides such as silver (Ag) and titanium dioxide (TiO2)but also provide antibacterial effect that able to enhance the nanoparticle performance.

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Biological synthesis of ZnO nanoparticles using C. albicans and studying their catalytic performance in the synthesis of steroidal pyrazolines

Arabian Journal of Chemistry ◽

10.1016/j.arabjc.2013.05.004 ◽

2017 ◽

Vol 10 ◽

pp. S1530-S1536 ◽

Cited By ~ 42

Author(s):

Shamsuzzaman ◽

Ashraf Mashrai ◽

Hena Khanam ◽

Rezq Naji Aljawfi

Keyword(s):

Zno Nanoparticles ◽

Catalytic Performance ◽

Biological Synthesis

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Decolorization of Azodyes by Chemically and Biosynthesized ZnO Nanoparticles

10.21203/rs.3.rs-722394/v1 ◽

2021 ◽

Author(s):

Jasmine Swain ◽

Padmashree Kulkarni ◽

Suba Manuel

Keyword(s):

Zno Nanoparticles ◽

Waste Water Treatment ◽

Industrial Effluents ◽

Dye Decolorization ◽

Precipitation Method ◽

Biological Synthesis ◽

Zno Nps ◽

Vis Spectroscopy ◽

Co Precipitation ◽

Biological Methods

Abstract Dye contaminants in industrial effluents contribute significantly to environmental pollution. Conventional waste water treatment methods are expensive and energy consuming. These limitations could be overcome by the use of nanobioremediation processes. The present work was an effort to study decolorization of azodyes by ZnO nanoparticles (NPs). Rubine GDB (R-GDB) and Congo Red (CR) were the azodyes selected for the study. The ZnO NPs were successfully synthesized by chemical and biological methods. Chemical synthesis of ZnO NPs was carried out by co-precipitation method; biological synthesis was done using bacteria Bacillus subtilis. The synthesized nanoparticles were characterized by UV-Vis Spectroscopy, SEM and XRD. The UV spectrophotometer showed peaks in the range of 300-400 nm. SEM pictures indicated the presence of NPs in the size of 100nm-200nm. XRD results were analyzed on the basis of the peaks that were seen. EDX analysis showed the presence of Zn particles and oxygen particles majorly. Decolorization efficiency was evualated by calculating % decolorization by Meyer’s method. Chemically synthesized NPs showed maximum % decolorization of the R-GDB (89.058 %) and CR (88.888%). The biosynthesized NPs showed the least decolorization (R- GDB -18 % and CR 20.357 %). However, moderate percentages of decolorization of both the azodyes were observed when a combination of the NPs was used (R-GDB -36.072 % and CR- 39.824 %). Nanoparticles showed good potential for the decolorization of the azo dyes. With further optimization of the parameters the present findings show that dye decolorization by chemically synthesized ZnO NPs could be used as a probable nanobioremediation approach to treat wastewaters.

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Biologically synthesized of Au/Pt/ZnO nanoparticles using Arctium lappa extract and cytotoxic activity against leukemia

Biomedical Microdevices ◽

10.1007/s10544-020-00526-z ◽

2020 ◽

Vol 22 (4) ◽

Author(s):

Renata Dobrucka ◽

Aleksandra Romaniuk-Drapała ◽

Mariusz Kaczmarek

Keyword(s):

Cytotoxic Activity ◽

Zno Nanoparticles ◽

Leukemia Cells ◽

Biological Synthesis ◽

Arctium Lappa ◽

Force Microscopy ◽

Cultivation Time ◽

Atomic Force ◽

Transmission Electron ◽

M Phase

AbstractThe main objective of this work was to assess the cytotoxic activity of Au/Pt/ZnO nanoparticles synthesized using Arctium lappa extract against leukemia. The Au/Pt/ZnO nanoparticles obtained as a result of biological synthesis were characterized by UV-Vis, Scanning (SEM) and Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFM). The applied methods showed that the size of nanoparticles ranged from 10 to 40 nm. This work also assessed the cytotoxicity of Au/Pt/ZnO nanoparticles by means of MTT assay, and analyzed apoptosis as well as the influence of the cultivation time and concentration of Au/Pt/ZnO nanoparticles on the percentage of dead cells. The studies showed that the percentage of dead leukemia cells increased with the cultivation time and concentration of Au/Pt/ZnO nanoparticles. There was observed an increase in the percentage of cells in the G2/M phase, which suggests the stoppage of G2/M leading to cell death. The cytotoxicity of Au/Pt/ZnO nanoparticles determined by means of the MTT test indicated that the viability of leukemia cells practically disappeared when the concentration of the tested nanoparticles was 10 mol.

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