Biofunctionalization and biological synthesis of the ZnO nanoparticles: The effect of Raphanus sativus (white radish) root extract on antimicrobial activity against MDR strain for wound healing applications

2019 ◽  
Vol 100 ◽  
pp. 101-106 ◽  
Author(s):  
A.B.V. Kiran Kumar ◽  
Edugulla Sre Saila ◽  
Prachi Narang ◽  
Mallelakari Aishwarya ◽  
Rebha Raina ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zno Nanoparticles ◽  
Raphanus Sativus ◽  
Biological Synthesis ◽  
Root Extract ◽  
Radish Root ◽  
White Radish

scholarly journals In Vitro Antifungal Efficacy of White Radish (Raphanus sativus L.) Root Extract and Application as a Natural Preservative in Sponge Cake

Processes ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 549
Author(s):  
Huynh Hoang Duy ◽  
Pham Thi Kim Ngoc ◽  
Le Thi Hong Anh ◽  
Dong Thi Anh Dao ◽  
Duy Chinh Nguyen ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Fungal Species ◽  
Root Extract ◽  
Sponge Cake ◽  
Radish Root ◽  
Raphanus Sativus L ◽  
White Radish

The study attempts the optimization of the total flavonoid content (TFC) and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) antioxidant activity of the white radish (Raphanus sativus L.) root ethanolic extract (WRE) with regard to several parameters including ethanol concentration, the ratio of solvent/material, temperature and extraction time. Then antifungal analysis of WRE was performed against four fungal species including Aspergillus flavus NBRC 33021, Aspergillus niger NBRC 4066, Aspergillus clavatus NBRC 33020, and Fusarium solani NBRC 31094. At the WRE concentration of 75 mg/mL, diameters of inhibition zone were 9.11 ± 1.5, 19.55 ± 1.68, 17.72 ± 0.25, and 17.50 ± 0.73 mm respectively against the four examined species. Minimum fungal concentration (MFC) values of WRE against the four species were 30, 10, 20 and 30 mg/mL respectively. Evaluation of the sponge cake added with white radish root extract suggested that shelf-life of the sponge cake is 8 weeks in air-cooled condition (20 °C) and two weeks in warm condition (37 °C).

Investigations on the antimicrobial activity and wound healing potential of ZnO nanoparticles

2019 ◽  
Vol 479 ◽  
pp. 1169-1177 ◽  
Author(s):  
M. Kaushik ◽  
R. Niranjan ◽  
Ramar Thangam ◽  
Balaraman Madhan ◽  
V. Pandiyarasan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zno Nanoparticles

Role of Zno Nanoparticles in Enhancing the Antimicrobial Property of Nitrocellulose Lacquer Wood Finish

2019 ◽  
Vol 12 (1) ◽  
pp. 91-98
Author(s):  
Shikhar Shukla ◽  
Shailendra Kumar ◽  
Ismita Nautiyal ◽  
Kishan Kumar V.S
Keyword(s):  
Antimicrobial Activity ◽  
Zno Nanoparticles ◽  
Antimicrobial Property ◽  
Economic Loss ◽  
Analysis Data ◽  
Wood Products ◽  
Mould Growth ◽  
Neem Oil ◽  
Nano Zno ◽  
Aesthetic Appearance

Background: Mould growth on coated wood products during shipment is the foremost apprehension for most of the wood industries in India. Well finished wood products tend to get deposited by staining moulds superficially in high humidity conditions during shipment. Though wood disfiguring fungi (stain moulds) normally have no significant effect on the mechanical properties of timber, they only affect the aesthetic appearance of the material without destructing the wood cell wall. This causes a huge economic loss to the manufacturer due to disfigurement of the appearance of wood products. Methods: The aim of this work is to test and enhance antimicrobial property of Nitrocellulose lacquer wood finish to prevent mould deposition on coated wood products. The work was carried out to screen and evaluate the antimicrobial activity of commercial Nitrocellulose lacquer. Several patents regarding antimicrobial activity of nano metal oxides have been visited. In order to enhance the mould resistance of this coating, its combinations with neem oil and ZnO nanoparticles were prepared and tested against the growth of staining moulds: Aspergillus niger and Penicillium sp. on the wood substrate of Melia dubia. The study was carried out for 500 hours under favorable conditions (Temperature and Relative Humidity (RH)) for the mould growth. The growth was analysed on the basis of surface area affected by stain moulds on coated wood samples. Results: The growth analysis data inferred that the ZnO nanoparticles had significant effect against the growth of stain moulds. The presence of ZnO nanoparticles increased the mould growth resistance of Nitrocellulose lacquer coating to a great extent compared to the ability of neem oil for the same. This finding demonstrates the potential use of nano ZnO for increasing the effectiveness of NC lacquer against mould growth without hampering its other properties. Conclusion: 1 % nano ZnO concentration in Nitrocellulose lacquer wood coating increased its efficacy against staining mould growth by 95 %.

scholarly journals Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shahrbanoo Hamedi ◽  
M. Mehdi Afsahi ◽  
Ali Riahi-Madvar ◽  
Ali Mohebbi
Keyword(s):  
Kinetic Parameters ◽  
Flow Rate ◽  
Air Flow ◽  
Cost Effective ◽  
Industrial Applications ◽  
Interactive Effects ◽  
Root Extract ◽  
Air Flow Rate ◽  
Spouted Bed ◽  
Radish Root

AbstractThe main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.

scholarly journals The Designer Antimicrobial Peptide A-hBD-2 Facilitates Skin Wound Healing by Stimulating Keratinocyte Migration and Proliferation

2018 ◽  
Vol 51 (2) ◽  
pp. 647-663 ◽  
Author(s):  
Bobin Mi ◽  
Jing Liu ◽  
Yi Liu ◽  
Liangcong Hu ◽  
Yukun Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Structural Stability ◽  
Cell Counting ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
High Sodium

Background/Aims: Antimicrobial peptides are effective promoters of wound healing but are susceptible to degradation. In this study, we replaced the GIGDP unit on the N-terminal of the endogenous human antimicrobial peptide hBD-2 with APKAM to produce A-hBD-2 and analyzed the effect on wound healing both in vitro and in vivo. Methods: The effects of A-hBD-2 and hBD-2 on cytotoxicity and proliferation in keratinocytes were assessed by Cell Counting Kit-8 assay. The structural stability and antimicrobial activity of hBD-2 and A-hBD-2 were evaluated against Staphylococcus aureus. RNA and proteins levels were evaluated by real-time PCR and western blotting, respectively. Cell migration was evaluated using a transwell assay. Cell cycle analysis was performed by flow cytometry. Wound healing was assessed in Sprague-Dawley rats. Epidermal thickness was evaluated by hematoxylin and eosin staining. Results: We found that hBD-2 exhibited cytotoxicity at high concentrations and decreased the structural stability in the presence of high sodium chloride concentrations. A-hBD-2 exhibited increased structural stability and antimicrobial activity, and had lower cytotoxicity in keratinocytes. A-hBD-2 increased the migration and proliferation of keratinocytes via phosphorylation of EGFR and STAT3 and suppressed terminal differentiation of keratinocytes. We also found that A-hBD-2 elicited mobilization of intracellular Ca2+ and stimulated keratinocytes to produce pro- and anti-inflammatory cytokines and chemokines via phospholipase C activation. Furthermore, A-hBD-2 promoted wound healing in vivo. Conclusion: Our data suggest that A-hBD-2 may be a promising candidate therapy for wound healing.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA

2017 ◽  
Vol 10 (11) ◽  
pp. 52
Author(s):  
Amita Shobha Rao ◽  
Shobha Kl ◽  
Prathibha Md’almeida ◽  
Kiranmai S Rai
Keyword(s):  
Antimicrobial Activity ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Root Extract ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
E Coli ◽  
Multidrug Resistant Strain

  Objective: Infections caused by Gram-negative bacteria are important causes of morbidity and mortality. Extracts of plants and herbs such as Clitorea ternatea are used as diuretic. This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), clinical strains of Klebsiella pneumoniae, and Candida albicans.Methods: The agar well-diffusion method was done using Mueller Hinton agar and Sabouraud’s dextrose agar. The microorganism grown in peptone water was inoculated into culture medium. 4 mm diameter well punched into the agar was filled with 20 μl of aqueous and alcoholic root extracts C. ternatea extracts in various concentrations (100-25 μg/ml). The plates were incubated and antimicrobial activity was evaluated.Results: Aqueous root extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition against E. coli (ATCC 25922) 18 mm, P. aeruginosa (ATCC 27853) 14 mm, multidrug resistant strain of K. pneumoniae 15 mm. Alcoholic extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition of 35 mm against E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) 22 mm, and multidrug resistant strain of K. pneumoniae 28 mm. C. albicanswas resistant to both extract of C. ternatea root. Conclusions: Alcoholic extract of C. ternatea is a better antibacterial agent against multidrug resistant Klebsiella species and other Gram-negative pathogens. Further, studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.

scholarly journals Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Ana Flávia da Silva ◽  
Marisa de Oliveira Lopes ◽  
Cláudio Daniel Cerdeira ◽  
Ingridy Simone Ribeiro ◽  
Isael Aparecido Rosa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Raphanus Sativus ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Dry Extract ◽  
Antioxidant And Antimicrobial Activities

The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

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