scholarly journals Biosynthesis of zinc oxide nanoparticles by using fruits extracts of Ananas Comosus and its antibacterial activity

2019 ◽  
Vol 15 (2) ◽  
pp. 268-273 ◽  
Author(s):  
Raja Adibah Raja Ahmad ◽  
Zawati Harun ◽  
Mohd Hafiz Dzarfan Othman ◽  
Hatijah Basri ◽  
Muhamad Zaini Yunos ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Metallic Nanoparticles ◽  
Ananas Comosus ◽  
Biological Synthesis ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
X Ray

Biosynthesis of metallic nanoparticles using plants, enzymes, and microorganism have been known as eco-friendly alternatives to conventional physical and chemical methods. Recently, the biological synthesis of nanoparticles has been a keen interest amongst researchers and scientist due to its simple technique, eco-friendliness, non-toxic, inexpensive and potential to perform in antibacterial activity. Thus, in this current work, the synthesis of zinc oxide (ZnO) nanoparticles using reduction agent from fruit extracts of Ananas Comosus is reported. The biosynthesized zinc oxide was characterized using Field Emission Scanning Electron Microscope (FESEM) with Energy Dispersive X-ray analysis (EDX), UV-Vis absorption spectroscopy and X-ray diffraction (XRD). The average size of the nanoparticles was found to be in the range of 30-57nm. The antibacterial activity of ZnO nanoparticles was carried out via agar diffusion method against pathogenic organisms. It is observed that the biosynthesized ZnO in the process has the efficient antibacterial activity. In conclusion, the green synthesis of zinc oxide nanoparticles using the fruit extract of Ananas Comosus is considered as a potential additive to substitute other metal oxides such as silver (Ag) and titanium dioxide (TiO2)but also provide antibacterial effect that able to enhance the nanoparticle performance.

scholarly journals Synthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Lippia adoensis (Koseret) and Evaluation of Its Antibacterial Activity

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Meron Girma Demissie ◽  
Fedlu Kedir Sabir ◽  
Gemechu Deressa Edossa ◽  
Bedasa Abdisa Gonfa
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Lippia Adoensis

The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.

scholarly journals Green Synthesis, Characterization of Zinc Oxide Nanoparticles and their Incorporation into Glass Ionomer Cement for Inhibition of Streptococcus mutans

2021 ◽  
Vol 33 (3) ◽  
pp. 515-520
Author(s):  
N. Ahalya ◽  
P. Dhamodhar ◽  
A.D. Vaishnavi
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Glass Ionomer Cement ◽  
Oxide Nanoparticles ◽  
Glass Ionomer ◽  
Uv Visible ◽  
Ionomer Cement

In present study, zinc oxide nanoparticles were synthesised using Syzygium aromaticum and characterized using UV visible spectroscopy, SEM, XRD and FTIR techniques. The characteristic hexagonal structure of the ZnO nanoparticles was confirmed through XRD analysis. The UV-Visible spectrum showed a strong absorbance at 366 nm confirming the presence of ZnO and the peak at 496 cm-1 in FTIR indicated the Zn-O stretch bond. Average size of the zinc oxide nanoparticles obtained from SEM analysis was found to be 86 nm. The zinc oxide nanoparticles exhibited better antibacterial activity than clove extract, when tested against clinical isolates of Streptococcus mutans. The nanoparticles incorporated into the dental restorative material, glass ionomer cement (GIC) were tested against S. mutans and exhibited better antibacterial activity than clove extract. Glass ionomer cement (GIC) did not exhibit antibacterial activity alone, but the incorporation of ZnO nanoparticles into GIC significantly improved antibacterial activity. Hence, ZnO embedded GIC is a promising material in restorative dentistry for preventing the recurrence of dental caries.

scholarly journals Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

2021 ◽  
Vol 11 ◽  
Author(s):  
Elsayim Rasha ◽  
Manal M. Alkhulaifi ◽  
Monerah AlOthman ◽  
Ibrahim Khalid ◽  
Elnagar Doaa ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Aspergillus Niger ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.

scholarly journals Biogenic Synthesis of Zinc Oxide (ZnO) Nanoparticles Using a Fungus (Aspargillus niger) and Their Characterization

2019 ◽  
Vol 11 (2) ◽  
pp. 119 ◽  
Author(s):  
Aisha Shamim ◽  
Tariq Mahmood ◽  
Monis Bin Abid
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Metallic Nanoparticles ◽  
Physical Method ◽  
Biological Synthesis ◽  
Zno Nps ◽  
Biogenic Synthesis ◽  
Time Saving ◽  
X Ray ◽  
Stabilizing Agents

Nanoparticles are ultrafine structures with dimensions less than 100 nm. Nanoparticles have diverse applications. There are three important methods of fabrication of nanoparticles namely physical, chemical and biological methods. Physical method is a top down strategy for the fabrication of nanoparticles. It is energy intensive and time consuming. A chemical method is simple, but is expensive and requires expensive chemicals with high purity and also involves hazards of contaminations. Biological synthesis is very simple, cheap and environment friendly, requiring no expensive chemicals, temperature and is time saving. Plants and microorganisms are commonly used in this method. These are available everywhere. In the present work we synthesized Zinc Oxide (ZnO) nanoparticles by biological method using Aspargillus niger and zinc chloride (ZnCl2) as precursors. Biogenic synthesis of metallic nanoparticles by fungi is a safe and economical process because of formation of stable and small sized nanoparticles. Fungal biomass secretes proteins which act as reducing and stabilizing agents. The synthesized nanoparticles were characterized by XRD (X-Ray Diffraction), SEM (Scanning Electron Microscopy), UV-Vis (Ultraviolet, Visible) and EDX (Energy Dispersive X-Ray) techniques. Their size was in nm range and morphology of synthesized ZnO NPs was hexagonal. The ZnO nanoparticles are one of the most versatile materials and are used in cosmetics and in Bioenergy production, as a catalyst and as antibacterial material.

Preparation of zinc oxide nanoparticles using punica granatum and elettaria cardamomum fruit extract and determining its antibacterial activity against lactobacillus

2020 ◽  
Vol 11 (3) ◽  
pp. 3372-3376
Author(s):  
Shilpa Merlyn Jose ◽  
Hannah.R ◽  
Rajeshkumar S
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Metal Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Metal Oxide Nanoparticles ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Fruit Extract ◽  
Elettaria Cardamomum

To determine the antibacterial activity of Zinc oxide nanoparticles synthesised using Punica granatum and Elettaria cardamomum fruit extract against Lactobacillus. Punica granatum and Elettaria cardamomum have been known for their antimicrobial, antioxidant and anti-inflammatory activity. The phytochemicals present in these fruits have experimented for the preparation of various metal and metal oxide nanoparticles. Zinc oxide is a widely used metal oxide nanoparticle known for its good antimicrobial activity against a host of microbes. The current study was conducted to determine its effect against Lactobacillus, a bacteria known for its role in the progression of dental caries. Preparation of fruit extract mediated zinc oxide nanoparticles. Determining the characteristics of the nanoparticles using UV spectroscopy and SEM. Analysing the activity of these nanoparticles against Lactobacillus using agar well diffusion method. The zone of inhibition increased if the concentration of the fruit mediated zinc oxide nanoparticles increased. But it was incomparable to the standards. Hence, further studies need to be conducted using different concentration of Punica granatum and Elettaria cardamomum to determine the optimum fruit extract required for the preparation of the nanoparticles. The resultant nanoparticles can be used as an effective antimicrobial agent against Lactobacillus

Antimicrobial Activity of Synthesized Zinc Oxide Nanoparticles using Ajuga bracteosa Leaf Extract

2021 ◽  
pp. 275-280
Author(s):  
M.C. Purohit ◽  
Anuj Kandwal ◽  
Reena Purohit ◽  
A.R. Semwal ◽  
Parveen Shama ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Metallic Nanoparticles ◽  
Leaf Extract ◽  
Trichoderma Viride ◽  
Colour Change ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Aspergillus Fumigates

Nanoscience and nanotechnology has attracted a lot of attention because of its wide variety of applications. Plant based metallic nanoparticles revolutionized the health sector with targeting nano drug to cure different ailments. Living beings are known to be susceptible to microbial attack followed by multidrug resistance of microorganism put the necessitates for searching more efficient methods of drug delivery or drug production. In the present study, we report the green synthesis of stable hexagonally shaped zinc oxide nanoparticles from leaf extract of Ajuga bracteosa and their antimicrobial efficacy against the selected bacterial (Streptococcus pneumonia, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa) and fungal (Aspergillus fumigates and Trichoderma viride) strains by using agar well diffusion method. Initial colour change and surface-plasmon-resonance (SPR) absorbance bands between 349 nm gave support to the synthesis of zinc oxide nanoparticles. These nanoparticles were further characterized by XRD, EDX, TEM and FTIR techniques. XRD analysis showed that nanoparticles are crystalline in nature. TEM measurements showed that nanoparticles are hexagonally shaped with their average size less than 27 nm. FTIR spectra confirms the presence of phytochemicals which were responsible for reducing, capping and stabilizing the nanoparticles. Antimicrobial results of the synthesized ZnO nanoparticles has indicated the good potential of nanoparticles against all tested microorganism in the present study.

Biosynthesis of ZnO Nanostructures Using Azadirachta indica Leaf Extract and Their Effect on Seed Germination and Seedling Growth of Tomato: An Eco-Friendly Approach

2020 ◽  
Vol 15 (11) ◽  
pp. 1412-1422
Author(s):  
Nishat Arshi ◽  
Y. Prashanthi ◽  
Tentu Nageswara Rao ◽  
Faheem Ahmed ◽  
Shalendra Kumar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Seed Germination ◽  
Azadirachta Indica ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
The Impact

In this study, we report synthesis of Zinc oxide nanoparticles using simple chemical and green methods. The ZnO nanoparticles were synthesized using leaf extract of Azadirachta indica (neem) as reducing agent. The as obtained product was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray analysis (EDAX) and TEM techniques. XRD analysis confirms that ZnO nanoparticles were crystalline having hexagonal Wurtzite structure with (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0) and (1 1 2) planes. SEM analyses show that the as synthesized ZnO NPs were in the form of agglomerates and no other impurity peak was found in the EDS. TEM analyses confirm that the size of the nanoparticle was approx. 50 nm. Here in, we investigate the effect of chemical and green synthesized zinc oxide nanoparticles on germination and growth of lycopersicum esculentus (tomato) using petri plate seed germination method in loamy sand soil. The impact of concentration of applied ZnO nanoparticles via green synthesis and chemical methods were analyzed. Results revealed that green synthesized Zinc oxide nanoparticles showed maximum growth of seedling as compared to chemically synthesized Zinc oxide nanoparticles, bulk ZnO and control. After 50 days of tomato growth analysis, it was recognized that ZnO NPs can be a good green synthetic fertilizer by increasing shoot length, wet weight, dry weight and yield over conventional control. Hence, green method is found to be more effective.

scholarly journals Effect of Zinc Oxide Nanoparticles on Loaded Antibiotics Against Multidrug-Resistant Acinetobacter spp.

2021 ◽  
Vol 8 (2) ◽  
pp. 51-56
Author(s):  
Behnaz Shokrollahi ◽  
Akram Sadat Tabatabaee Bafroee ◽  
Tayebeh Saleh
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Metal Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray

Background: Metal oxide nanoparticles (NPs) have shown promising efficacy for combating bacterial resistance due to their antibacterial properties. This research investigated the effect of zinc oxide NPs (ZnO-NPs) on the antibacterial activity of conventional antibiotics including ciprofloxacin (CIP), cefotaxime (CTX), and colistin (CST) against multidrug-resistant Acinetobacter isolates. Methods: The disc diffusion method was performed to detect the pattern of antibiotic resistance in isolates. The synthesized ZnO-NPs via the solvothermal method were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). Finally, the broth microdilution technique was conducted to demonstrate the antibacterial activity of CIP, CTX, and CST antibiotics with and without a sub-inhibitory concentration of ZnO-NPs. Results: XRD, EDS, and FESEM results confirmed the crystalline structure of ZnO-NPs, and the average size was 100±58.68 nm. All isolates were discovered to be of multidrug-resistant (MDR) type and fully susceptible to CST. The antibacterial activity of CTX and CIP was restored when combined with a sub-inhibitory level of ZnO-NPs (0.25 mg/L), and the highest activity was obtained at the concentrations of 32 µg/mL CTX and 8 µg/ mL CIP. Eventually, ZnO-NPs showed a synergistic effect on the antibacterial properties of CST against MDR Acinetobacter. Conclusions: This research indicated that the combination of ZnO-NPs with some common antibiotics can be considered as a novel strategy for reducing the spread of antibiotic-resistant bacteria.

scholarly journals Camellia sinensis (Green Tea) Mediated Synthesis of Zinc Oxide Nanoparticles and Detect its Antibacterial Activity against Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii

2017 ◽  
Vol 11 (1) ◽  
pp. 34-40
Author(s):  
Israa Ali Zaidan Al-Ogaidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Acinetobacter Baumannii ◽  
Green Tea ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Pathogenic Bacteria ◽  
Oxide Nanoparticles

In the current study, synthesis and characterization of Zinc oxide nanoparticles (ZnONPs) and its application as anti-pathogenic bacteria were investigated.  ZnO which has been prepared by using aqueous of green tea leaves extract (Camellia sinensis) as a reducing agent. The wavelength range was measured by Ultraviolet–visible spectroscopy (UV-Vis) for monitoring the formation of the nanoparticles, which showed sharp peak at 360 nm. The average size and shape of the nanoparticles were detected by using Atomic Force Microscopy (AFM) which was 88 nm with spherical shape. Fourier transform–infrared (FTIR). FT-IR spectra was documented for the ZnO nanoparticles synthesized by green tea extract to detect the biomolecules involved in the synthesis process. The antibacterial activity of crystal Zinc Oxide (ZnO) nanoparticles was explored against pathogenic bacteria that included Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. The antibacterial test was conducted in solid media using different concentrations of ZnO and disk diffusion method, 100 µg/ml presented the best antibacterial activity, and further studies on the damage of bacterial genomic DNA of Escherichia coli and Acinetobacter baumannii were carried out using gel electrophoresis exposed the DNA fragment bands, this activity may be caused by the interactions between the surface charge of cell and nanoparticles. Reactive oxygen species (ROS) properties of the particles might disturb cell wall and great antimicrobial action

scholarly journals High-Yield Synthesis of Zinc Oxide Nanoparticles from Bicontinuous Microemulsions

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
S. López-Cuenca ◽  
L. A. Pérez Carrillo ◽  
M. Rabelero Velasco ◽  
R. Díaz de León ◽  
H. Saade ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
High Yield ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Electronic Microscopy ◽  
X Ray ◽  
Hydrolysis Of

The high-yield synthesis of zinc oxide (ZnO) primary nanoparticles with high purity and with diameters between 6 and 22 nm using bicontinuous microemulsions is reported in this work. The ZnO nanoparticles were made by hydrolysis of Zn(NO3)2with NaOH aqueous solution and precipitation, followed by calcination of the precipitate. Higher yields and productivities of ZnO nanoparticles were obtained compared to values produced with w/o micremulsions reported in the literature. Particles were characterized by transmission electronic microscopy (TEM), X-ray diffraction, and atomic absorption spectroscopy.

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