Contents of phytochemical constituents and antioxidant activity of 19 garlic (Allium sativum L.) parental lines and cultivars

2014 ◽  
Vol 55 (2) ◽  
pp. 138-147 ◽  
Author(s):  
Shiva Ram Bhandari ◽  
Moo Kyoung Yoon ◽  
Jung-Ho Kwak
Author(s):  
Mahinder Partap ◽  
Raghbir Chand Gupta ◽  
Saroj Kumar Pradhan

Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.


FEMS Microbes ◽  
2022 ◽  
Author(s):  
Yimeslal Atnafu Sema ◽  
Teshale Areda Waktola

Abstract In Ethiopia, the impacts of malaria continue to cause a many number of morbidity and mortality that accounts to most outpatient observations. Ethiopia recently designed to attain nationwide malaria control by 2030 by beginning sub-national elimination in districts with low malaria transmission. However, the rises of drug-resistant parasites, especially Plasmodium falciparum hinder the malaria containment strategies. Plasmodium falciparum and Plasmodium vivax, dispersed all over the Ethiopia and accounting for 60% and 40% of malaria cases respectively. The aim of this report was to overview the phytochemical constituents, diversity and effect of some compound extracts on drug resistant plasmodium species. Many plant species, a total 200 identified by 82 studies, are used in traditional malaria treatments throughout the country. Allium sativum, Croton macrostachyus and Carica papaya were the more frequently used medicinal plants species. There are so many phytochemicals constituents found in medicinal plants used to treat malaria. Alkaloids, Flavonoids, Phenolics, Terpenoid and Glycosides are the most reported for their effective activity on drug resistant malaria.


Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.


Author(s):  
Shanmugam S ◽  
Usha Rani V ◽  
Pradeep B V

Objective: The present study was on the analysis of the phytochemical constituents of Coleus forskohlii which was collected from five different areas in Tamil Nadu, where it is grown for commercial purposes.Methods: C. forskohlii tubers were collected from five areas (Thiruvannamalai, Kanchipuram, Kallakuruchi, Salem, and Vandalur) of Tamil Nadu. Dried tubers were extracted with different solvents such as aqueous, ethanol, and acetone (polar) and petroleum and chloroform (non-polar). Extracts were evaluated for total phenol and flavonoid content and antioxidant activity evaluation by 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction.Results: The highest DPPH radical scavenging activity (87.6%) was observed in ethanol extract of rhizome collected from Thiruvannamalai area. Total phenol and flavonoid contents were found to be, respectively, 38.82±0.22 mg gallic acid equivalents/g and 21.34±0.32 quercetin equivalent/g.Conclusion: The study clearly indicated the antioxidative potential of different extracts of C. forskohlii and this property may be useful in the preparation of drug formulations, health tonics, and cosmetics.


2012 ◽  
Vol 2 (2) ◽  
pp. S539-S542 ◽  
Author(s):  
Narender Prasad D ◽  
B Ganga Rao ◽  
E Sambasiva Rao ◽  
T Mallikarjuna Rao ◽  
VS Praneeth D

Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.


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