Pulicaria laciniata (Coss and Kral): Effects of Different Extraction Solvents on Phytochemical Screening and Antioxidant Activity

2021 ◽  
pp. 425-430
Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
The Other ◽  
Phytochemical Screening ◽  
Butanol Extract ◽  
Metal Chelating ◽  
Phytochemical Investigation ◽  
Ic50 Value ◽  
Extraction Solvents ◽  
Phytochemical Constituents

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.

Phytochemical screening and antioxidant activity of a edible, medicinally important plant taxa Mollugo pentaphylla L. (Molluginaceae)

2016 ◽  
Vol 5 (07) ◽  
pp. 4707
Author(s):  
Debasree Ghosh ◽  
Prarthita Samanta ◽  
Amal Kumar Mondal* ◽  
Sanjukta Mondal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Volatile Oil ◽  
The Other ◽  
Flavonoid Glycosides ◽  
Phytochemical Screening ◽  
High Concentration ◽  
Medicinal Value ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents

Mollugo pentaphylla L. is a medicinally important plants in India. Many rural peoples are consumed this plants as a food mainly leaf and stem portion. But the other parts like stem and root of this plant is also has a great medicinal value. The objective of the present study to evaluate the identification of phytochemical constituents that are present on this plants. In qualitative analysis, the phytochemical compounds such as carbohydrates, proteins, polyphenols, carotenoid, ascorbic acid, alkaloid, tannin, flavonoid, glycosides, volatile oil etc. Besides this, these plants also have antioxidant potential in high concentration.

scholarly journals Phytochemical Screening, Antioxidant Activity and Phenolic Content of Different Plant Parts of Brucea javanica (L.)

2019 ◽  
Vol 14 (2) ◽  
pp. 33-43
Author(s):  
Hamidah Jaafar Sidek ◽  
Hidayatul Atiqah Abd Karim ◽  
Zurina Mahmud
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
The Other ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Brucea Javanica ◽  
Plant Parts ◽  
Ic50 Value

In this study, phytochemical screening, antioxidant activity as well as phenolic content in the leaves,twigs and barks of Brucea javanica (L.) were determined using standard phytochemical screeningmethod, DPPH radical scavenging assay and Folin-Ciocalteu reagent method, respectively. Methanolicextracts of these different plant parts were prepared by maceration method. Phytochemical screeningrevealed the presence of flavonoids, terpenoids and tannins in all plant parts. This test also revealed thepresence of alkaloids and saponins in all plant parts except they were absent in the twigs and barks parts,respectively. On the other hand, steroids was absent in all plant parts. DPPH method was used toevaluate the antioxidant properties of the plant parts by measuring the absorbance at 517 nm. Theantioxidant activity was then compared with standard ascorbic acid. Among all parts, methanolic extractof leaves of Brucea javanica (L.) exhibited the highest antioxidant activity at the concentration of 100ppm with 86.19 ± 0.20% inhibition which was comparable with standard ascorbic acid with 97.62 ±0.13% inhibition at the same concentration. The IC50 value of the methanolic extract of leaves was 54.52± 0.16 ppm while the other parts were more than 100 ppm. All plant parts showed IC50 value higher thanthe standard ascorbic acid which recorded IC50 of 9.04 ± 0.09 ppm. The methanolic extract of the leavesalso exhibited the highest total phenolic content which was 105.58 ± 0.21 mg GAE/g extract compared tothe other parts. This result correlated well with the higher antioxidant activity exhibited by themethanolic extract of the leaves. Thus, the leaves part of methanolic extract of Brucea javanica (L.)exhibited the highest antioxidant activity compared to the other plant parts. Keywords: Brucea javanica (L.), simaroubaceae, phytochemical screening, antioxidant activity, phenolic content

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Effect of packaging on phenols, flavonoids and antioxidant characteristics of mechanical cabinet dried wild pomegranate (Punica granatum L.) arils

2021 ◽  
Vol 13 (1) ◽  
pp. 101-109
Author(s):  
Abhimanyu Thakur ◽  
NS Thakur ◽  
Hamid ◽  
Sunakshi Gautam
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Punica Granatum ◽  
Total Phenols ◽  
Antioxidant Power ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Temperature Conditions ◽  
Metal Chelating Activity ◽  
Punica Granatum L

Wild pomegranate (Punica granatum L.) fruit arils with high acid content are processed into dried arils (anardana) which is used as an ingredient in various medicinal formulations and culinary preparations. The various phenols and flavonoids are responsible for the antioxidant activity of anardana which signifies its functional benefits. The advanced packaging techniques like vacuum packaging, can retain higher antioxidant activity characteristics during storage. So, the present studies were carried out to compare the effect of packaging on total phenols, flavonoids, DPPH (2, 2-diphenyl-1-picrylhydrazyl) antioxidant activity, metal chelating activity, FRAP (ferric reducing antioxidant power) and reducing power of anardana prepared in mechanical cabinet drier. Anardana was packed in different packaging materials and stored under ambient and refrigerated temperature conditions. The overall effect of storage during 12 months revealed that there was a significant (p< 0.05) decrease in total phenols (180.95 to 161.76 mg GAE/100 g), flavonoids (40.60 to 32.66 mg QuE/100 g), DPPH antioxidant activity (61.23 to 56.13%), metal chelating activity (22.25 to 19.37%), FRAP (34.60 to 31.73 ?M Fe2+/100 g) and reducing power (0.610 to 0.521) which was observed less in anardana packed in ALPV (aluminium laminated pouches with vacuum) followed by ALP (aluminium laminated pouches) and gunny bags. Due to slower rates of degradation of various antioxidant compounds, the changes after 12 months of storage were lower under refrigerated conditions. The total phenols, flavonoids and DPPH antioxidant activity were reduced  from 180.95 to 167.24 and 156.28 mg GAE/100 g, 40.60 to 34.95 and 30.37 mg QuE/100 g and 61.23 to 57.99 and 54.27 % in refrigerated and ambient temperature conditions, respectively.

scholarly journals STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 384
Author(s):  
Caroline Wijaya ◽  
Berna Elya ◽  
Arry Yanuar
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Cassia Fistula ◽  
Kinetic Assay ◽  
Tyrosinase Inhibitory Activity ◽  
Water Fraction ◽  
Ic50 Value ◽  
Phytochemical Constituents

Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition ofdopachrome formation resulting from the L-DOPA-tyrosinase reaction.Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistulaleaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract containedalkaloids, flavonoids, glycosides, phenols, and tannins.Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

Polyphenol Composition and Antioxidant Activity of Andrographis paniculata L. Nees

2017 ◽  
Vol 13 (4) ◽  
pp. 33-46 ◽  
Author(s):  
Praveen N
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
High Performance ◽  
Reducing Power ◽  
High Performance Liquid Chromatographic ◽  
Andrographis Paniculata ◽  
Total Phenolic ◽  
Butanol Extract ◽  
The Individual

The aim of the present study was to establish the polyphenolic profile and antioxidant activity of the leaves of Andrographis paniculata (kalmegh) that could be potentially used for the benefit of mankind. An ultra high performance liquid chromatographic method was used to identify and quantify the individual phenolic compounds of the kalmegh leaves. A total of 20 polyphenolic compounds were identified and quantified in kalmegh leaves, including hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other group of phenolic compounds. The UPLC analysis of the phenolic compounds revealed that salicylic acid was the dominant phenolic compound present in the leaves extract followed by gallic acid, chlorogenic acid, veratric acid and protocatechuic acid. The kalmegh leaves were extracted with 4 different solvents (ethyl acetate, methanol, butanol and water) and screened for total phenolic content (TPC) and antioxidant activity. The antioxidant activity of kalmegh was assessed by evaluating the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and phosphomolybdenum assay. Ethyl acetate and butanol extract exhibited the highest phenolic

scholarly journals PHYTOCHEMICAL STUDY AND IN VITRO ANTIOXIDANT ACTIVITIES OF HAMMADA SCOPARIA EXTRACTS FROM SOUTHEASTERN ALGERIA

2018 ◽  
Vol 11 (10) ◽  
pp. 187
Author(s):  
Benkherara Salah ◽  
Bordjiba O
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Aerial Part ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Trolox Equivalent

Objective: This study was carried out to determine the phytochemical constituents and to evaluate the antioxidant potential of the aerial part extracts of Hammada scoparia (Pomel) Iljin to validate the medicinal potential of this Algerian plant species.Methods: Crude extracts were prepared by cold maceration with absolute methanol and distilled water. Preliminary phytochemical screening is carried out to detect the presence of the major secondary metabolites using qualitative characterization methods. Quantitative estimation of total phenols, total flavonoids, flavanols, flavonols, and condensed tannins contents is performed using gallic acid, rutin, catechin, and quercetin as standards. In vitro antioxidant activity was evaluated by the free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity by scavenging of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay, and the ferric reducing power assay (ferric reducing antioxidant power).Results: Phytochemical screening showed the presence of alkaloids, flavonoids, tannins, terpenes and sterols, saponins, and anthocyanins. Total phenols were present more in aqueous crude extract (ACE) with 336.756±0.855 mg gallic acid equivalent/g DM. Total flavonoids and flavonols were more abundant in methanolic crude extract (MCE) than ACE. However, condensed tannins and flavanols were present less in MCE with only 0.958±0.052 and 4.547±0.055 mg CE/g DM, respectively. The ACE of this plant species had greater antioxidant activity than the other extract by DPPH and ABTS assays (35.823±0.129 and 51.323±0.394 mg trolox equivalent/g DM). The better ferric reducing power (2060.535±2.566 mM Fe+2/g DM) was also recorded with the same extract.Conclusion: ACE of aerial part of H. scoparia (Pomel) Iljin showed a high amount of secondary metabolites. The obtained results confirmed that the extracting solvent influenced the antioxidant property estimations of this plant. Hence, the ACE can be further exploited further for in vitro and in vivo research work.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

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