scholarly journals Salvia ceratophylla L. from South of Jordan: new insights on chemical composition and biological activities

2020 ◽  
Vol 10 (5) ◽  
pp. 307-316
Author(s):  
Mohammad Sanad Abu-Darwish ◽  
Célia Cabral ◽  
Zulfigar Ali ◽  
Mei Wang ◽  
Shabana I. Khan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Vero Cells ◽  
Gas Chromatography Mass Spectrometry ◽  
No Production ◽  
Main Constituent ◽  
Microbial Infections

Abstract In Jordan, Salvia ceratophylla L. is traditionally used in the treatment of cancer, microbial infections, and urinary disorders. This study aimed: (1) to chemically characterize S. ceratophylla essential oil (EO) from South Jordan, by gas chromatography (GC) and gas chromatography-mass spectrometry (GC–MS); and (2) to evaluate in vitro the cytotoxic, anti-inflammatory, and antiprotozoal activities of the EO, it’s predominant components, and the hexane (A), ethyl acetate (B), methanol (C) and crude-methanol extracts (D). The analysis revealed that the EO has 71 compounds, with linalool (54.8%) as main constituent. Only the hexane extract (A) showed some cytotoxic activity against SK-MEL, KB, BT-549, SK-OV-3, LLC-PK1 and VERO cells lines with IC50 between 60 and > 100 µg/mL. The EO inhibited NO production (IC50 90 µg/mL) and NF-κB activity (IC50 38 µg/mL). The extracts A, B, and D inhibited NO production and NF- κB activity with IC50 between 32 and 150 µg/mL. Linalool considerably inhibited NO production (IC50 18 µg/mL). The extracts tested did not exhibit antileishmanial activity. Regarding antitrypanosomal activity, the EO exhibited significant results with IC50 2.65 µg/mL. In conclusion, Jordan S. ceratophylla EO represents a rich source of linalool and bears a promising therapeutic potential for further antitrypanosomal drug development.

scholarly journals BIOLOGICAL ACTIVITIES OF GARCINIA MANGOSTANA

2017 ◽  
Vol 10 (9) ◽  
pp. 272 ◽  
Author(s):  
Jebalsy Lalitha ◽  
Prince Clarance P ◽  
Jerin Sales T ◽  
Mary Archana A ◽  
Agastian P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Garcinia Mangostana

  Objective: The aim of the present study was to investigate in vitro antibacterial, antifungal, and antioxidant activities of different solvent extracts of Garcinia mangostana leaves.Methods: The powdered leaf was subjected to sequential extraction using hexane, ethyl acetate (EA), and methanol. The extracts were subjected to quantitative and qualitative phytochemical analysis, antimicrobial, and antioxidant activities. The best solvent extract was subjected to gas chromatography–mass spectrometry (GC-MS) analysis.Results: The highest activity was recorded in EA extract which was subjected to GC-MS analysis revealing the presence of squalene (17.09%).Conclusion: From this present study, we conclude that EA is the best solvent for extracting antimicrobial and antioxidant compounds from the leaves of G. mangostana.

scholarly journals Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography–mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kajsa Roslund ◽  
Markku Lehto ◽  
Pirkko Pussinen ◽  
Kari Hartonen ◽  
Per-Henrik Groop ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Analysis ◽  
Bacterial Volatiles

AbstractWe have measured the volatile fingerprints of four pathogenic oral bacteria connected to periodontal disease and dental abscess: Porphyromonas gingivalis (three separate strains), Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia. Volatile fingerprints were measured in vitro from the headspace gas of the bacteria cultured on agar. Concrete identification of new and previously reported bacterial volatiles were performed by a combination of solid phase microextraction (SPME) and offline gas chromatography–mass spectrometry (GC–MS). We also studied the effect of the reduced electric field strength (E/N) on the fragmentation patterns of bacterial volatiles in online proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). We aimed to discover possible new biomarkers for the studied oral bacteria, as well as to validate the combination of GC–MS and PTR-MS for volatile analysis. Some of the most promising compounds produced include: 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ), indole, and a cascade of sulphur compounds, such as methanethiol, dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). We also found that several compounds, especially alcohols, aldehydes and esters, fragment significantly with the PTR-MS method, when high E/N values are used. We conclude that the studied oral bacteria can be separated by their volatile fingerprints in vitro, which could have importance in clinical and laboratory environments. In addition, using softer ionization conditions can improve the performance of the PTR-MS method in the volatile analysis of certain compounds.

Gas chromatography-mass spectrometry fingerprint and in vitro cytotoxic studies of Rubus steudneri leaf fractions against michigan cancer foundation-7 breast cancer cell line

2021 ◽  
Vol 17 (5) ◽  
pp. 54
Author(s):  
RaghavendraLakshmana Shetty Hallur ◽  
ChaitanyaV. N L. Motamarri ◽  
PrashithKekuda T. Ramamoorthy ◽  
ChetanD Murthy ◽  
RavikumarPatil H. Siddappa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Studies

scholarly journals In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

2018 ◽  
Vol 7 (9) ◽  
pp. 283 ◽  
Author(s):  
Sherif Hassan ◽  
Kateřina Berchová-Bímová ◽  
Miroslava Šudomová ◽  
Milan Malaník ◽  
Karel Šmejkal ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Clinical Practice ◽  
Essential Oil ◽  
Biological Activities ◽  
Structural Parameters ◽  
Antihypertensive Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Ic50 Values ◽  
Main Component

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 µg/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 µg/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 µg/mL compared with that of cisplatin (IC50 = 10.08 µg/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 µg/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 µg/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 µg/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

Identification of Bioactive Phytocomponents of Hydroalcoholic Extract of Enhalus acoroides by Gas Chromatography- Mass Spectrometry Analysis

2021 ◽  
pp. 6511-6515
Author(s):  
S. Navaith Ahmed ◽  
P. Kalaivani ◽  
P. Amudha ◽  
B. Usharani
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activities ◽  
Marine Species ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hydroalcoholic Extract ◽  
Spectrometry Analysis ◽  
Enhalus Acoroides ◽  
Chromatography Mass Spectrometry

Sea grass are good source of potent drug which has medicinal properties and able to cure human disease. One such marine plant is the Enhalus acoroides which belong to hydrocharitaeceae family. This marine species are widely distributed in the tropics of Indian and western Pacific Ocean and the species were collected from Ramanathapuram district for further process. This study reveals about the bioactive components present in Enhalus acoroides and identified its biological activity by Gas chromatography Mass spectrometry analysis using hydroalcoholic extract. The compounds present in Enhalus acoroides are Benzoic acid, 2- methyl 7- oxa bicyclol heptanes, 1, 3 Nonadiene, silane, ethoxytriethyl. Biological activities of the compounds present in the sample include antioxidant, hypocholesterolemic, antihypertensive, anti-inflammatory, anti-microbial, antiviral and anti-hepatotoxic effect.

scholarly journals GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

2018 ◽  
Vol 11 (12) ◽  
pp. 247
Author(s):  
Kuladip Gurav ◽  
Varsha Jadhav (rathod)
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

scholarly journals Chemical Composition and Biological Activities of Essential Oils of Pinus patula

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ismail Amri ◽  
Hamrouni Lamia ◽  
Samia Gargouri ◽  
Mohsen Hanana ◽  
Mariem Mahfoudhi ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Pinus Patula ◽  
Flame Ionization ◽  
Total Oil

Essential oils isolated from needles of Pinus patula by hydrodistillation were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Thirty-eight compounds were identified, representing 98.3% of the total oil. The oil was rich in monoterpene hydrocarbons (62.4%), particularly α-pinene (35.2%) and β-phellandrene (19.5%). The in vitro antifungal assay showed that P. patula oil significantly inhibited the growth of 9 plant pathogenic fungi. The oil, when tested on Sinapis arvensis, Lolium rigidum, Phalaris canariensis and Trifolium campestre, completely inhibited seed germination and seedling growth of all species. Our preliminary results showed that P. patula essential oil could be valorized for the control of weeds and fungal plant diseases.

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