scholarly journals BIOLOGICAL ACTIVITIES OF GARCINIA MANGOSTANA

2017 ◽  
Vol 10 (9) ◽  
pp. 272 ◽  
Author(s):  
Jebalsy Lalitha ◽  
Prince Clarance P ◽  
Jerin Sales T ◽  
Mary Archana A ◽  
Agastian P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Garcinia Mangostana

  Objective: The aim of the present study was to investigate in vitro antibacterial, antifungal, and antioxidant activities of different solvent extracts of Garcinia mangostana leaves.Methods: The powdered leaf was subjected to sequential extraction using hexane, ethyl acetate (EA), and methanol. The extracts were subjected to quantitative and qualitative phytochemical analysis, antimicrobial, and antioxidant activities. The best solvent extract was subjected to gas chromatography–mass spectrometry (GC-MS) analysis.Results: The highest activity was recorded in EA extract which was subjected to GC-MS analysis revealing the presence of squalene (17.09%).Conclusion: From this present study, we conclude that EA is the best solvent for extracting antimicrobial and antioxidant compounds from the leaves of G. mangostana.

scholarly journals Salvia ceratophylla L. from South of Jordan: new insights on chemical composition and biological activities

2020 ◽  
Vol 10 (5) ◽  
pp. 307-316
Author(s):  
Mohammad Sanad Abu-Darwish ◽  
Célia Cabral ◽  
Zulfigar Ali ◽  
Mei Wang ◽  
Shabana I. Khan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Vero Cells ◽  
Gas Chromatography Mass Spectrometry ◽  
No Production ◽  
Main Constituent ◽  
Microbial Infections

Abstract In Jordan, Salvia ceratophylla L. is traditionally used in the treatment of cancer, microbial infections, and urinary disorders. This study aimed: (1) to chemically characterize S. ceratophylla essential oil (EO) from South Jordan, by gas chromatography (GC) and gas chromatography-mass spectrometry (GC–MS); and (2) to evaluate in vitro the cytotoxic, anti-inflammatory, and antiprotozoal activities of the EO, it’s predominant components, and the hexane (A), ethyl acetate (B), methanol (C) and crude-methanol extracts (D). The analysis revealed that the EO has 71 compounds, with linalool (54.8%) as main constituent. Only the hexane extract (A) showed some cytotoxic activity against SK-MEL, KB, BT-549, SK-OV-3, LLC-PK1 and VERO cells lines with IC50 between 60 and > 100 µg/mL. The EO inhibited NO production (IC50 90 µg/mL) and NF-κB activity (IC50 38 µg/mL). The extracts A, B, and D inhibited NO production and NF- κB activity with IC50 between 32 and 150 µg/mL. Linalool considerably inhibited NO production (IC50 18 µg/mL). The extracts tested did not exhibit antileishmanial activity. Regarding antitrypanosomal activity, the EO exhibited significant results with IC50 2.65 µg/mL. In conclusion, Jordan S. ceratophylla EO represents a rich source of linalool and bears a promising therapeutic potential for further antitrypanosomal drug development.

scholarly journals ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES

2019 ◽  
Vol 12 (1) ◽  
pp. 289 ◽  
Author(s):  
Samreen Fatema ◽  
Milind Ubale ◽  
Mazahar Farooqui ◽  
Pathan Mohd Arif
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Results ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Study

Objective: The main objective of the present work is to carry out the biological activity, gas chromatography-mass spectrometry (GC-MS) studies for the possible compounds present in Vitex negundo.Methods: The aqueous extract of V. negundo Linn. was screened for biological activities such as antimicrobial, antituberculosis (TB), antimalarial, and antioxidant activities. The GC-MS analysis was carried out.Results: The result shows that leaf extract is effective against Escherichia Coli and Bacillus subtitus while negative results for anti-TB and anti-malarial activity. The antioxidant activity of the leave extract is excellent.Conclusion: The compounds present in the leaf extract of V. negundo are responsible for possessing the biological activity.

scholarly journals ANALYSIS OF BIOLOGICAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDY OF CONVENTIONAL EXTRACTION OF VITEX NEGUNDO LINN. LEAVES

2019 ◽  
Vol 12 (1) ◽  
pp. 289
Author(s):  
Samreen Fatema ◽  
Milind Ubale ◽  
Mazahar Farooqui ◽  
Pathan Mohd Arif
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Biological Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Results ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Study

Objective: The main objective of the present work is to carry out the biological activity, gas chromatography-mass spectrometry (GC-MS) studies for the possible compounds present in Vitex negundo.Methods: The aqueous extract of V. negundo Linn. was screened for biological activities such as antimicrobial, antituberculosis (TB), antimalarial, and antioxidant activities. The GC-MS analysis was carried out.Results: The result shows that leaf extract is effective against Escherichia Coli and Bacillus subtitus while negative results for anti-TB and anti-malarial activity. The antioxidant activity of the leave extract is excellent.Conclusion: The compounds present in the leaf extract of V. negundo are responsible for possessing the biological activity.

scholarly journals PHYTOCHEMICAL ANALYSIS OF PORTULACA OLERACEA AND PORTULACA QUADRIFIDA EXTRACTS USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY

2018 ◽  
Vol 11 (9) ◽  
pp. 204 ◽  
Author(s):  
Trupti P Durgawale ◽  
Chitra C Khanwelkar ◽  
Pratik P Durgawale
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Agents ◽  
Essential Fatty Acids ◽  
Portulaca Oleracea ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Ethanolic Extracts

Objective: The two plant species Portulaca oleracea and Portulaca quadrifida are commonly known as purslane and chickweed, respectively. They are typically consumed as salad or pickle. Traditional systems of medicine from Africa and China have described these plants belonging to family Portulacaceae as remedies against a host of diseases. Recent pharmacological investigations have revealed the importance of these plants as sources of antioxidants, essential fatty acids, and even antimicrobial agents. The objective of this study was phytochemical analysis and comparison of ethanolic extracts of these two species of Portulaca.Methods: The ethanolic extracts of both the species were prepared using Soxhlet extraction and were analyzed using gas chromatography coupled with mass spectrometry (GC–MS). Furthermore, the ethanolic extracts of fresh and dried whole plant of P. oleracea and seed of P. oleracea were studied.Results: The phytochemical constituents of ethanolic extracts of P. oleracea and P. quarifida were found to be quite different from one another and contained beneficial polyunsaturated fatty acids, alkaloids among other beneficial chemical species.Conclusion: The results of the study could be further used by researchers to assess the beneficial properties of both these species for in vitro and in vivo experiments.

scholarly journals PHYTOCHEMICAL ANALYSIS, IN VITRO ANTIOXIDANT POTENTIAL AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES OF DICRANOPTERIS LINEARIS

2016 ◽  
pp. 220 ◽  
Author(s):  
Kalpana Devi Rajesh ◽  
Subramani Vasantha ◽  
Annamalai Panneerselvam ◽  
Nakulan Valsala Rajesh ◽  
Narayanaperumal Jeyathilakan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Western Ghats ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Chromatography Mass Spectrometry ◽  
Dicranopteris Linearis

<p>ABSTRACT<br />Objective: The aim of the study was to analyze qualitative and quantitative phytochemicals, evaluate in vitro antioxidant properties and determine the<br />bioactive compounds in extracts of Dicranopteris linearis (Burm.f.) Underw. collected from Western Ghats of Kanyakumari district.<br />Methods: The qualitative, quantitative phytochemical, and in vitro antioxidant analysis were performed using standard procedures. The bioactive<br />compounds were analyzed using gas chromatography-mass spectrometry (GC-MS) instrument.<br />Results: The qualitative phytochemical analysis studied in aqueous, acetone, chloroform, ethanol, and petroleum ether solvent extract showed acetone<br />had strong positivity to express the 12 phytoconstituents studied except anthocyanin when compared to other solvent extracts. The quantitative<br />phytochemistry revealed considerable amount of terpenoids (97.0±1.15 mg/g), tannins (30.8±0.44 mg tannic acid equivalents/gram), phenols<br />(28.6±0.33 mg gallic acid equivalents/gram), and flavonoids (8.50±0.29 mg quercetin equivalent/g) in decreasing order of concentrations. The in<br />vitro antioxidant activity of aqueous, ethanol, acetone, chloroform, and petroleum ether suggested that the extract of DL has prominent antioxidant<br />prospective against various free radicals such as 2,2-diphenyl-1-picrylhydrazyl while butylated hydroxy toluene being the standard antioxidant used.<br />The GC-MS analysis displayed the presence of 11 bioactive compounds each belonging to various categories of phytochemicals such as terpenoids,<br />flavonoids, phenols, and fatty acid derivatives.<br />Conclusion: The results indicate that D. linearis (Burm.f.) Underw. present in the Western Ghats of Kanyakumari is an effective scavenger of free<br />radicals and has the potential to be used as a natural antioxidant which is attributed to the rich presence of secondary metabolites.<br />Keywords: Dicranopteris linearis (Burm.f.) Underw., Phytochemistry, Antioxidant activity, Gas chromatography-mass Spectrometry.</p>

scholarly journals Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography–mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kajsa Roslund ◽  
Markku Lehto ◽  
Pirkko Pussinen ◽  
Kari Hartonen ◽  
Per-Henrik Groop ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Analysis ◽  
Bacterial Volatiles

AbstractWe have measured the volatile fingerprints of four pathogenic oral bacteria connected to periodontal disease and dental abscess: Porphyromonas gingivalis (three separate strains), Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia. Volatile fingerprints were measured in vitro from the headspace gas of the bacteria cultured on agar. Concrete identification of new and previously reported bacterial volatiles were performed by a combination of solid phase microextraction (SPME) and offline gas chromatography–mass spectrometry (GC–MS). We also studied the effect of the reduced electric field strength (E/N) on the fragmentation patterns of bacterial volatiles in online proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). We aimed to discover possible new biomarkers for the studied oral bacteria, as well as to validate the combination of GC–MS and PTR-MS for volatile analysis. Some of the most promising compounds produced include: 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ), indole, and a cascade of sulphur compounds, such as methanethiol, dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). We also found that several compounds, especially alcohols, aldehydes and esters, fragment significantly with the PTR-MS method, when high E/N values are used. We conclude that the studied oral bacteria can be separated by their volatile fingerprints in vitro, which could have importance in clinical and laboratory environments. In addition, using softer ionization conditions can improve the performance of the PTR-MS method in the volatile analysis of certain compounds.

scholarly journals Phytochemical Analysis and Potential Biological Activities of Essential Oil from Rice Leaf

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 546 ◽  
Author(s):  
Truong Minh ◽  
Tran Xuan ◽  
Truong Van ◽  
Yusuf Andriana ◽  
Tran Viet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Atmospheric Pressure Chemical Ionization ◽  
Biological Activities ◽  
Methyl Ricinoleate ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Chemical Compositions ◽  
Rice Leaf ◽  
Rice Leaves

Although many investigations on phytochemicals in rice plant parts and root exudates have been conducted, information on the chemical profile of essential oil (EO) and potent biological activities has been limited. In this study, chemical compositions of rice leaf EO and in vitro biological activities were investigated. From 1.5 kg of fresh rice leaves, an amount of 20 mg EO was obtained by distillation and analyzed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI) to reveal the presence of twelve volatile constituents, of which methyl ricinoleate (27.86%) was the principal compound, followed by palmitic acid (17.34%), and linolenic acid (11.16%), while 2-pentadecanone was the least (2.13%). Two phytoalexin momilactones A and B were first time identified in EO using ultra-performance liquid chromatography coupled with electrospray mass spectrometry (UPLC/ESI-MS) (9.80 and 4.93 ng/g fresh weight, respectively), which accounted for 7.35% and 3.70% of the EO, respectively. The assays of DPPH (IC50 = 73.1 µg/mL), ABTS (IC50 = 198.3 µg/mL), FRAP (IC50 = 700.8 µg/mL) and β-carotene oxidation (LPI = 79%) revealed that EO possessed an excellent antioxidant activity. The xanthine oxidase assay indicated that the anti-hyperuricemia potential was in a moderate level (IC50 = 526 µg/mL) as compared with the standard allopurinol. The EO exerted potent inhibition on growth of Raphanus sativus, Lactuca sativa, and two noxious weeds Echinochloa crus-galli, and Bidens pilosa, but in contrast, the growth of rice seedlings was promoted. Among the examined plants, the growth of the E. crus-galli root was the most inhibited, proposing that constituents found in EO may have potential for the control of the problematic paddy weed E. crus-galli. It was found that the EO of rice leaves contained rich phytochemicals, which were potent in antioxidants and gout treatment, as well as weed management. Findings of this study highlighted the potential value of rice leaves, which may provide extra benefits for rice farmers.

Gas chromatography-mass spectrometry fingerprint and in vitro cytotoxic studies of Rubus steudneri leaf fractions against michigan cancer foundation-7 breast cancer cell line

2021 ◽  
Vol 17 (5) ◽  
pp. 54
Author(s):  
RaghavendraLakshmana Shetty Hallur ◽  
ChaitanyaV. N L. Motamarri ◽  
PrashithKekuda T. Ramamoorthy ◽  
ChetanD Murthy ◽  
RavikumarPatil H. Siddappa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Studies

scholarly journals In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

2018 ◽  
Vol 7 (9) ◽  
pp. 283 ◽  
Author(s):  
Sherif Hassan ◽  
Kateřina Berchová-Bímová ◽  
Miroslava Šudomová ◽  
Milan Malaník ◽  
Karel Šmejkal ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Clinical Practice ◽  
Essential Oil ◽  
Biological Activities ◽  
Structural Parameters ◽  
Antihypertensive Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Ic50 Values ◽  
Main Component

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 µg/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 µg/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 µg/mL compared with that of cisplatin (IC50 = 10.08 µg/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 µg/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 µg/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 µg/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

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