scholarly journals Grafting collagen on poly (lactic acid) by a simple route to produce electrospun scaffolds, and their cell adhesion evaluation

2016 ◽  
Vol 13 (4) ◽  
pp. 375-387 ◽  
Author(s):  
Alida Ospina-Orejarena ◽  
Ricardo Vera-Graziano ◽  
Maria Monica Castillo-Ortega ◽  
Juan Paulo Hinestroza ◽  
Mabel Rodriguez-Gonzalez ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Poly Lactic Acid ◽  
Simple Route ◽  
Electrospun Scaffolds

scholarly journals Effect of nano-hydroxyapatite on protein adsorption and cell adhesion of poly(lactic acid)/nano-hydroxyapatite composite microspheres

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
H. M. C. Suboda Wijerathne ◽  
Dong Yan ◽  
Bin Zeng ◽  
Yanping Xie ◽  
Hongchao Hu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Protein Adsorption ◽  
Poly Lactic Acid ◽  
Composite Microspheres ◽  
Hydroxyapatite Composite

scholarly journals Efficient differentiation and polarization of primary cultured neurons on poly(lactic acid) scaffolds with microgrooved structures

2019 ◽  
Author(s):  
Asako Otomo ◽  
Mahoko Takahashi Ueda ◽  
Toshinori Fujie ◽  
Arihiro Hasebe ◽  
Yosuke Okamura ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Cortical Neurons ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Structural Features ◽  
Poly Lactic Acid ◽  
Adhesion Properties ◽  
Expression Of Genes ◽  
Cultured Cortical Neurons

AbstractSynthetic biodegradable polymers including poly(lactic acid) (PLA) are attractive cell culture substrates because their surfaces can be micropatterned to support cell adhesion. The cell adhesion properties of a scaffold mainly depend on its surface chemical and structural features; however, it remains unclear how these characteristics affect the growth and differentiation of cultured cells or their gene expression. In this study, we fabricated two differently structured PLA nanosheets: flat and microgrooved. We assessed the growth and differentiation of mouse primary cultured cortical neurons on these two types of nanosheets after pre-coating with poly-D-lysine and vitronectin. Interestingly, prominent neurite bundles were formed along the grooves on the microgrooved nanosheets, whereas thin and randomly extended neurites were only observed on the flat nanosheets. Comparative RNA sequencing analyses revealed that the expression of genes related to postsynaptic density, dendritic shafts, and asymmetric synapses was significantly and consistently up-regulated in cells cultured on the microgrooved nanosheets when compared with those cultured on the flat nanosheets. These results indicate that microgrooved PLA nanosheets can provide a powerful means of establishing a culture system for the efficient and reproducible differentiation of neurons, which will facilitate future investigations of the molecular mechanisms underlying the pathogenesis of neurological disorders.

Diamond nanoparticles into poly (lactic acid) electrospun fibers: Cytocompatible and bioactive scaffolds with enhanced wettability and cell adhesion

2016 ◽  
Vol 183 ◽  
pp. 420-424 ◽  
Author(s):  
F.A.S. Pereira ◽  
G.N. Salles ◽  
B.V.M. Rodrigues ◽  
F.R. Marciano ◽  
C. Pacheco-Soares ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Poly Lactic Acid ◽  
Electrospun Fibers ◽  
Diamond Nanoparticles ◽  
Bioactive Scaffolds

Cell Adhesion and Morphology in Porous Scaffold Based on Enantiomeric Poly(lactic acid) Graft-type Phospholipid Polymers

2002 ◽  
Vol 3 (6) ◽  
pp. 1375-1383 ◽  
Author(s):  
Junji Watanabe ◽  
Takahisa Eriguchi ◽  
Kazuhiko Ishihara
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Porous Scaffold ◽  
Poly Lactic Acid

scholarly journals Design of Functional Electrospun Scaffolds Based on Poly(glycerol sebacate) Elastomer and Poly(lactic acid) for Cardiac Tissue Engineering

2020 ◽  
Vol 6 (4) ◽  
pp. 2388-2400 ◽  
Author(s):  
Florence Flaig ◽  
Hélène Ragot ◽  
Alexandre Simon ◽  
Gaëlle Revet ◽  
Maria Kitsara ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Poly Lactic Acid ◽  
Electrospun Scaffolds

scholarly journals Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Michele Savaris ◽  
Gustavo L. Braga ◽  
Venina dos Santos ◽  
Glaucio A. Carvalho ◽  
Asdrubal Falavigna ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Adhesion ◽  
Ethylene Oxide ◽  
Wistar Rats ◽  
Histological Study ◽  
Surrounding Tissue ◽  
Poly Lactic Acid ◽  
Cytotoxicity Test

Biomaterials must meet certain fundamental requirements for their usage in living beings, such as biocompatibility, bifunctionality, and sterilizability, without having chemical and structural changes. The biocompatibility of poly(lactic acid) (PLA) films, shaped by compression, was evaluated after sterilization by ethylene oxide by a histological in vivo test with Wistar rats and cytotoxicity in cell adhesion in vitro. The cytotoxicity test was performed by the reduction of tetrazolium salt (MTT). Thermal and chemical changes in PLA films concerning the proposed sterilization process and characteristics were not observed to evidence polymer degradation due to sterilization. The analysis of the cytotoxicity by the MTT method has shown that the sterilized PLA films are not cytotoxic. The adhesion and proliferation of fibroblasts on PLA films were homogeneously distributed over the evaluation period, showing an elongated appearance with unnumbered cytoplasmic extensions and cell-cell interactions. By examining the biocompatibility in a histological study, a mild tissue inflammation was observed with the presence of fibrosis in the samples that had been exposed for 21 days in the rats’ bodies. PLA films sterilized with ethylene oxide did not exhibit cell adhesion in vitro and toxicity to the surrounding tissue in vivo and they may be used in future in vivo testing, according to histological findings in Wistar rats in the present study.

scholarly journals Thermal and dynamic mechanical behavior of poly(lactic acid) (PLA)‐based electrospun scaffolds for tissue engineering

2021 ◽  
pp. 51313
Author(s):  
Nicola Ciarfaglia ◽  
Antonio Laezza ◽  
Louise Lods ◽  
Antoine Lonjon ◽  
Jany Dandurand ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Mechanical Behavior ◽  
Poly Lactic Acid ◽  
Dynamic Mechanical ◽  
Electrospun Scaffolds ◽  
Dynamic Mechanical Behavior

Electrical Properties of Heat-Treated Poly-Lactic Acid

2011 ◽  
Vol 131 (5) ◽  
pp. 395-400 ◽  
Author(s):  
Toru Oi ◽  
Katsuyoshi Shinyama ◽  
Shigetaka Fujita
Keyword(s):  
Lactic Acid ◽  
Electrical Properties ◽  
Poly Lactic Acid ◽  
Heat Treated

Dielectric Breakdown Properties of Poly-Lactic Acid with Spherulites Growth

2014 ◽  
Vol 134 (4) ◽  
pp. 237-242
Author(s):  
Naru Matsugasaki ◽  
Katsuyoshi Shinyama ◽  
Shigetaka Fujita
Keyword(s):  
Lactic Acid ◽  
Dielectric Breakdown ◽  
Poly Lactic Acid
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