Biomarker System for Genetic Analysis of Stored Grain Pests Callosobruchus maculatus and Callosobruchus chinensis

Stored grain pests are discovered in food as immature stages, which further complicates the identification process. A DNA barcode dataset of some important pests that can be used for easy and confirm identification in stages of life is constructed. COI genes of three stored grain insect pests i.e,, Sitophilus oryzae, Callosobruchus chinensis and Oryzaephilus surinamensis were sequenced. The sequenced genes were submitted to NCBI GenBank and obtained accession numbers MG967331.1, MG967332.1, MG967333.1 and MK041216.1. BLAST analysis showed 99 to 100% homology with existing GenBank sequences. The nucleotide composition analysis revealed that the value of A+T (64.8%) is greater than G+C (35.2%). Genetic distance among four sequences of three store pests were ranged from 0.00293-0.32807. Phylogenetic analysis showed that these three species are originated from different clades. Haplotype analysis of mitochondrial COI gene of the stored grain insect pests showed high genetic diversity among them. C. chinensis, O. surinamensis and S. oryzae were separated from their common ancestor by 80, 73 and 64 mutational steps. These information may be helpful for attempting any successful control measures against the pest species. In conclusion, present author established the first DNA barcode dataset of three store grain pests and confirmed its efficiency for identifying these pests. Bangladesh J. Zool. 47(1): 1-11, 2019

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Evaluation of Some Botanicals against Callosobruchus chinensis L. Infesting Stored Chickpea Seeds and Bio-Chemical Analysis of Used Botanicals

International Journal of Bio-resource and Stress Management ◽

10.23910/1.2021.2416 ◽

2021 ◽

Vol 12 (6) ◽

pp. 679-686

Author(s):

Tumma Mounika ◽

◽

S. K. Sahoo ◽

D. Chakraborty ◽

◽

...

Keyword(s):

Management Strategies ◽

Datura Stramonium ◽

Adult Mortality ◽

Egg Laying ◽

Ocimum Sanctum ◽

Melia Azedarach ◽

Callosobruchus Chinensis ◽

Stored Grain ◽

Leaf Powder ◽

Stored Grain Pests

A study was carried out to evaluate the bio-efficacy of some botanicals against Callosobruchus chinensis L. in stored chickpea (Variety: Anuradha) in the year 2018–20. Different botanicals like Neem (Azadirachta indica), Melia (Melia azedarach), Datura (Datura stramonium) and Tulsi (Ocimum sanctum) were used. Among all the botanicals Neem leaf powder @ 6% performed better with minimum egg laying (64 eggs 5 females-1) compared to the other botanicals except the standard check with Deltamethrin @ 0.04%. Thereafter, 6% Melia leaf powder and 8% Datura leaf powder recorded 87.50 eggs 5 females-1 and 91.25 eggs 5 females-1, respectively. With regard to adult mortality, the best result was also obtained from the treatment Neem leaf powder @6% with 96.67% mortality in 5th day. Following the same trend, 6% Melia leaf powder and 8% Datura leaf powder exhibited 90% and 83.33% adult mortality, respectively, in 5th day. The estimation of total phenols and total antioxidants (IC-50) contents in Neem leaf powder (345.69 mg g-1 and 207.77 μg ml-1), Melia leaf powder (273.40 mg g-1 and 383.68 μg ml-1) and Datura Leaf Powder (213.62 mg g-1 and 405.77 μg ml-1) also confirms the findings of the bio-efficacy trial of the botanicals. Tulsi leaf powder @5% was least efficacious both in terms of egg laying by the females as well as adult mortality. These botanicals are locally available, economic, bio-degradable and safe to the environment. Therefore, they may be fitted in the Integrated Pest Management strategies against stored grain pests as seed protectants.

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Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

Journal of Scientific Research ◽

10.3329/jsr.v9i4.32589 ◽

2017 ◽

Vol 9 (4) ◽

pp. 359-366

Author(s):

Z. R. Sabuj ◽

A. Khatun ◽

M. Abdullah ◽

D. M. Badruddoza ◽

H. Ali ◽

...

Keyword(s):

Petroleum Ether ◽

Tribolium Castaneum ◽

Sitophilus Oryzae ◽

Stem Bark ◽

Callosobruchus Chinensis ◽

Ether Extract ◽

Stored Grain ◽

Chcl3 Extract ◽

Stored Grain Pests ◽

White Mulberry

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didnt show mortality.

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Toxicity of the essential oil of basil cultivars and hybrids and its repellent effect on stored grain pests

Bioscience Journal ◽

10.14393/bj-v36n2a2020-42266 ◽

2020 ◽

Vol 36 (2) ◽

Author(s):

Andréa Santos da Costa ◽

Maria Fátima de Arrigoni-BlanK ◽

Leandro Bacci ◽

Arie Fitzgerald Blank ◽

Péricles Barreto Alves ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Callosobruchus Maculatus ◽

Repellent Effect ◽

Stored Grain ◽

Methyl Cinnamate ◽

Stored Grain Pests

Essential oils have emerged as an alternative to synthetic insecticides in the control of stored grain pests. The toxicity and repellency of the essential oils of four basil cultivars and three basil hybrids and the monoterpenes linalool, citral, and (E)-methyl cinnamate were evaluated in the stored grain pests Callosobruchus maculatus and Sitophillus zeamais. The essential oils of the cultivar Genovese and the hybrid 'Genovese' x 'Maria Bonita' were more toxic to C. maculatus. Conversely, the essential oils of the cultivar Sweet Dani and the hybrid 'Cinnamom' x 'Maria Bonita' were more toxic to S. zeamais. Among the monoterpenes, (E)-methyl cinnamate was the most toxic to both pests, taking 0.14 and 0.34 µL.mL-1 to kill 50% of the C. maculatus and S. zeamais populations, respectively. All essential oils from cultivars, hybrids, and monoterpenes were repellent to S. zeamais, except for (E)-methyl cinnamate. For C. maculatus, this effect was lower, being citral the most repellent compound. Results demonstrate the insecticidal potential of the essential oil of O. basilicum and its monoterpenes in the control of stored grain pests.

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Genetic analysis of seed resistance to Callosobruchus chinensis and Callosobruchus maculatus in cowpea

Journal of Stored Products Research ◽

10.1016/j.jspr.2021.101783 ◽

2021 ◽

Vol 92 ◽

pp. 101783

Author(s):

Khin Thandar ◽

Kularb Laosatit ◽

Tarika Yimram ◽

Prakit Somta

Keyword(s):

Genetic Analysis ◽

Callosobruchus Maculatus ◽

Callosobruchus Chinensis ◽

Seed Resistance

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Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00418-1 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Tabassum Ara Khanum ◽

Salma Javed

Keyword(s):

Biological Control ◽

Tribolium Castaneum ◽

Biological Control Agent ◽

Economic Loss ◽

Research Area ◽

Control Agent ◽

Lasioderma Serricorne ◽

Stored Grain ◽

Insect Infestations ◽

Stored Grain Pests

Abstract Background Stored grain products suffer a considerable economic loss due to insect infestations. The resistance of pests to insecticide, and residues on crops are produced by the application of insecticide. Nowadays, incremental necessity has been occurred to find out alternatives to chemicals. As biological control agents, entomopathogenic nematodes (EPNs) attract attention increasingly in the research area recently. Results The aim of the study was to evaluate the pathogenicity of Pakistani isolates Steinernema bifurcatum and S. affine against the stored grain pests, Tribolium castaneum and Lasioderma serricorne, under laboratory conditions. Suspensions of nematodes were applied at 4 different concentrations 0, 50, 100, and 200 IJs/ml and 3 variable temperatures 15, 30, and 45°C. Pathogenicity rate was recorded after 48 h of application. The larval stage is found more susceptible than the adult. At 200 IJs, S. bifurcatum showed high mortality of L. serricorne larva (92%) and T. castaneum larva (93%), whereas S. affine showed 90 and 95% mortality of L. serricorne and T. castaneum larva, respectively, at 200 IJs. The results revealed that both species of EPN were able to control and reproduce on L. serricorne and T. castaneum. Maximum mortality was recorded at 45 °C from S. bifurcatum whereas S. affine at 15 °C. Conclusions Therefore, S. bifurcatum and S. affine could be suggested as a biological control agent for hot and cold climatic zones, respectively.

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