Prospects for Use of Biological Control of Insect and Mites for the Food Industry in North America

There are a number of strategies used to mitigate and control insect infestations in stored products and stored product facilities in North America and globally. Fumigation remains one of the main techniques used, particularly in bulk grain. Other techniques are also utilized effectively, such as the use of extreme temperatures and the use of biological control agents, but are mainly restricted to organic products and to Europe, respectively. Here, we review the past research conducted in the field of biological control for pests of stored products in North America and in Europe, its past and present successes in Europe, its challenges, and what we can learn from them to develop biological control as a viable option to problems of insect pests of stored products in North America.

Clay Nanotube Immobilization on Animal Hair for Sustained Anti-Lice Protection

Topical administration of drugs is required for the treatment of parasitic diseases and insect infestations; therefore, fabrication of nanoscale drug carriers for effective insecticide topical delivery is needed. Here we report the enhanced immobilization of halloysite tubule nanoclay onto semiaquatic capybaras which have hydrophobic hair surfaces as compared to their close relatives, land-dwelling guinea pigs, and other agricultural livestock. The hair surface of mammals varies in hydrophobicity having a cortex surrounded by cuticles. Spontaneous 1–2 µm thick halloysite hair coverages on the semi-aquatic rodent capybara, non-aquatic rodent guinea pig, and farm goats were compared. The best coating was found for capybara due to the elevated 5 wt% wax content. As a result, we suggest hair pretreatment with diluted wax for enhanced nanoclay adsorption. The formation of a stable goat hair coverage with a 2–3 µm halloysite layer loaded with permethrin insecticide allowed for long-lasting anti-parasitic protection, enduring multiple rain wettings and washings. We expect that our technology will find applications in animal parasitosis protection and may be extended to prolonged human anti-lice treatment.

Climate Change and Internet of Things Technologies—Sustainable Premises of Extending the Culture of the Amurg Cultivar in Transylvania—A Use Case for Târnave Vineyard

Known for its dry and semi-dry white wine, the Târnave vineyard located in central Transylvania is challenged by the current climate change, which has resulted in an increase of the period of active vegetation by approximately 15–20 days, the average annual temperature by 1–1.5 °C and also the amount of useful temperatures (useful thermal balance for the grapevine). Furthermore, the frost periods have been reduced. Transylvania is an important Romanian region for grapevine cultivation. In this context, one can use the climatic changes to expand their wine assortment by cultivating an autochthonous grapevine variety called Amurg. Amurg is a red grape cultivar homologated at SCDVV Blaj, which also homologated 7 cultivars and 11 clones. Because viticulture depends on the stability of meteorological and hydrological parameters of the growing area, its foundations are challenged by climate change. Grapevine production is a long time investment, taking at least five years before the freshly planted vines produce the desired quality berries. We propose the implementation of a climate change-based precision viticulture turn-key solution for environmental monitoring in the Târnave vineyard. This solution aims to evaluate the grapevine’s micro-climate to extend the sustainable cultivation of the Amurg red grapes cultivar in Transylvania with the final goal of obtaining Protected Designation of Origin (PDO) rosé and red wines from this region. Worldwide, the changing conditions from the existing climate (a 30-year average), used in the past hundred years to dictate local standards, such as new and erratic trends of temperature and humidity regimes, late spring freezes, early fall frosts, storms, heatwaves, droughts, area wildfires, and insect infestations, would create dynamic problems for all farmers to thrive. These conditions will make it challenging to predict shifts in each of the components of seasonal weather conditions. Our proposed system also aims to give a solution that can be adapted to other vineyards as well.

Yield and Quality of Ratoon Sugarcane Are Improved by Applying Potassium under Irrigation to Potassium Deficient Soils

The current study was carried out at the experimental farm of Rana Sugars Ltd., Buttar Seviyan, Amritsar, Punjab, India, to identify methods to improve the yield and quality of ratoon sugarcane in potassium-deficient soils. The treatments comprised two levels of irrigation, resulting in plants which either received sufficient water (I1) or were water-stressed (I2), and four rates of potassium (K) application: 0 (K1), 40 (K2), 80 (K3) and 120 (K4) kg K2O ha−1. The results showed that the irrigation levels did not influence crop parameters significantly, although all parameters presented higher values for I1-treated plots. Compared to the K1 (i.e., 0 kg ha−1 K fertiliser applied) treatment, the K2, K3 and K4 treatments yielded 11.16, 37.9 and 40.7%, respectively, higher millable canes and 1.25, 5.62 and 13.13% more nodes per plant, respectively. At 280 days after harvest of the first (plant) crop, the I1 treatment provided ratoons which were up to 15.58% higher than those obtained with the I2 treatment, with cane girths up to 7.69% wider and yields up to 7.29% higher than those observed with the I2 treatment. While the number of nodes per plant did not differ significantly between treatments, there were significant differences in other parameters. Quality parameters (with the exception of extraction percentage) were significantly enhanced by the K3 treatment. The benefit-to-cost ratio (B/C) was higher for the I1 treatment than for the I2, due to a reduced productivity associated with the I2 treatment. At both irrigation levels, the K3 treatment resulted in the highest quality parameters. K1-, K2- and K4-treated plots presented more instances of insect infestations than plots receiving the K3 treatment. Relative to the K3 plots, infestation by the early shoot borer (Chilo infuscatellus) was 18.2, 6.0 and 12.2% higher, respectively, in plots that underwent the K1, K2 and K4 treatments, while infestation by the top borer (Scirpophaga excerptalis) was 21.2, 9.21 and 14.0% higher, and that by the stalk borer (Chilo auricilius) was 10.7, 0 and 8.10% higher. Not all infestation differences between treatments were significant. Our research demonstrates that growing sugarcane in potassium-deficient soils with applications of 80 kg K2O ha−1 under irrigation should be recommended to increase yield and quality while minimising insect infestation and to implement sustainable ratoon sugarcane production.

Insect infestations in mealworm farming: the case of the pyralid moths

During the last decade the insect sector has witnessed a tremendous growth. A lot of scientific knowledge has been accumulated regarding insect production, processing and industrialisation. However, an issue that has been often overlooked is the management of insect pest infestations in insect production plants. Pyralid moths appear to be the most prevalent insect pest-species in mealworm farms. Although not directly affecting the mealworm larvae, these species can cause severe problems infesting the feed. Their presence in the production facility can lead to the contamination of the mealworm protein with moth protein and the disruption of sieving and processing due to the larval webbing. The management of moth infestations in insect rearing units is rather challenging, as most commonly applied insecticide-based control measures cannot be implemented due to their negative impact on insect production. Therefore, moth control should be based on the integration of several alternative strategies in a holistic approach that combines improved sanitation and hygiene with non-chemical control measures. The application of extreme temperatures, biological control, mating disruption and other control tools for the management of insect infestations in insect producing facilities is being discussed within this review.

Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)

Background Stored grain products suffer a considerable economic loss due to insect infestations. The resistance of pests to insecticide, and residues on crops are produced by the application of insecticide. Nowadays, incremental necessity has been occurred to find out alternatives to chemicals. As biological control agents, entomopathogenic nematodes (EPNs) attract attention increasingly in the research area recently. Results The aim of the study was to evaluate the pathogenicity of Pakistani isolates Steinernema bifurcatum and S. affine against the stored grain pests, Tribolium castaneum and Lasioderma serricorne, under laboratory conditions. Suspensions of nematodes were applied at 4 different concentrations 0, 50, 100, and 200 IJs/ml and 3 variable temperatures 15, 30, and 45°C. Pathogenicity rate was recorded after 48 h of application. The larval stage is found more susceptible than the adult. At 200 IJs, S. bifurcatum showed high mortality of L. serricorne larva (92%) and T. castaneum larva (93%), whereas S. affine showed 90 and 95% mortality of L. serricorne and T. castaneum larva, respectively, at 200 IJs. The results revealed that both species of EPN were able to control and reproduce on L. serricorne and T. castaneum. Maximum mortality was recorded at 45 °C from S. bifurcatum whereas S. affine at 15 °C. Conclusions Therefore, S. bifurcatum and S. affine could be suggested as a biological control agent for hot and cold climatic zones, respectively.

Pheromones and 25 years of pheromonotherapy: what are they and how do they work?

The capacity of animals to communicate via pheromones is long established and, for generations, pheromones have been unwittingly used by man to manage the behaviour of animals in agriculture — using the chemicals produced by an individual member of a species to alter the behaviour of another member of that species. More recently, insect pheromones have been used in managing insect infestations of crops. However, approximately 25 years ago the French veterinary surgeon, Patrick Pageat, began to investigate the production of pheromones in both farm and companion animals and how synthetic analogues of pheromones could be used to improve animal welfare, creating a new field in veterinary medicine — pheromonotherapy. This article aims to summarise the main developments in pheromonotherapy over the last 25 years.

Dieback and leaf spot in box elder (Acer negundo) caused by Exserohilum rostratum

Leaf spot and dieback were observed on box elder (Acer negundo) grown in a nursery in Tai'an city, Shandong Province, China, in 2019, with a disease incidence of 86%. The incidence of Exserohilum rostratum isolation was 75% from the shoots and 66.6% from the leaves of field-infected plants. Isolates were identified at the species level on the basis of morphological characteristics and through phylogenetic analysis of concatenated partial sequences of the internal transcribed spacer (ITS) region and cam, gapdh, tef1, rpb2, tub2, and his genes from the Exserohilum isolates. The effects of temperature on the mycelial growth of the E. rostratum isolates were also characterized. In greenhouse tests, seedlings inoculated with the pathogen exhibited systemic symptoms similar to those observed in the field. In pathogenicity experiments on shoots, wounded seedlings were observed to be blighted, suggesting that leaf spot and dieback may develop into more severe blight or dieback when high winds, sudden temperature drops, or insect infestations occur. To our knowledge, this is the first report of dieback and leaf spot caused by E. rostratum on a species of A. negundo.

Fungal entomopathogens: a systematic review

Background Apprehensions about the safety and the environment regarding the insecticidal application against insect infestations have directed our attention toward advancement of biological mediators so that they are assimilated into the concept of integrated pest management stratagems to develop a more practical approach for the management of insect pests. Management of insect pests by making use of biological approaches (such as fungal entomopathogens (EPF) or others which are antagonistic to insect population) provides a substitute approach which reduces the continuous use of chemical amalgams against insect pests. Main body The present review provides a framework of the present status of information on EPF as it identifies with their current use as biological control of pest infestations. To utilize a variety of biological control methodologies against insect hosts, it is essential to improve our comprehension of the ecology of EPF and also their role in nature. This article may assist us with understanding the virulence and the virulence factors related with EPF and present the latest developments and accomplishments in the significant field. We focus on recent instances of studies that show the overall patterns in interactions among insect pests and EPF prompting the advancement of epizootics. Also, we sum up the topical discoveries on current status of mycoinsecticides and propose future research needs. Conclusions As the current mechanism of fungal pathogenesis on insects is moderately slow and needs improvement, there is likewise the requirement for additional comprehension of the interactions among entomopathogens and insect pests so as to grow soundly planned procedures by identifying potential targets and via the improvement of fungal strains for improving the adequacy of these organisms in field applications.

Rock and Roll: RFID Tracking of Fluvial Bedload Transport and Interaction with Large Wood

<p>Bedload transport is a fundamental process by which coarse sediment is transferred through landscapes by river networks and is characterized by cyclic sequences of particle motion and rest. Bedload transport has many complex physical controls but may be well described stochastically by distributions of grain step length and rest time obtained through tracer studies. To date, none of these published tracer studies have specifically investigated the influence of large wood on distributions of step length or rest time, limiting the applicability of stochastic sediment transport models in these settings. Large wood is a major component of many forested rivers and is increasing because of disturbances such as wildfire and insect infestations as well as its use in rivers as part of ‘Natural Food Management’ (NFM) practice. This study aims to investigate and model the influence of large wood on grain-scale bedload transport. </p><p>St Louis Creek, an alpine stream in the Fraser Experimental Forest, Colorado, is experiencing increased wood loading resulting from the infestation of the mountain pine beetle in the past decades. We inserted 957 Passive Integrative Transponders (PIT) tagged cobbles in 2016 upstream of a wood loaded reach and measured and tagged > 20 pieces of large wood in the channel. We resurveyed the cobbles and wood on an annual basis after snowmelt, building distributions of rock-step lengths as well as observing any changes and transport of large wood. Additionally, a novel modelling approach based on linear mixed modelling (LMM) statistical approaches is implemented to establish the significance of wood and other factors on probability of particle entrainment, deposition and step length.</p><p>Tracer sediments accumulated both up and downstream of large wood pieces, with LMM analysis confirming a reduction in the probability of entrainment of tracers closer to wood. In addition, when tracers were remobilised, their subsequent step lengths were shorter the closer they were deposited to large wood. In 2019, large wood significantly reduced the step lengths of tracer particles, forcing premature deposition of tracers. This study demonstrates the role of large wood in influencing bedload transport in alpine stream environments, with implications for both natural and anthropogenic addition of wood debris in fluvial environments.</p>