scholarly journals Assessment of Chlorella vulgaris, Scenedesmus obliquus, and Oocystis minuta for removal of sulfate, nitrate, and phosphate in wastewater

2020 ◽  
Vol 11 (3) ◽  
pp. 311-326 ◽  
Author(s):  
Sheriff Olalekan Ajala ◽  
Matthew L. Alexander
Keyword(s):  
Chlorella Vulgaris ◽  
Scenedesmus Obliquus ◽  
Biomass Productivity ◽  
Growth Conditions ◽  
Phosphate Removal ◽  
Biofuel Production ◽  
Protein Accumulation ◽  
Contaminant Removal ◽  
Biochemical Component ◽  
Sulfate Removal

AbstractApplication of wastewater for algal biomass production can not only lead to production of thousands of tons of biomass for subsequent biofuel production, but also can provide for significant removal of contaminants in wastewater. The aim of the present study is to evaluate the growth, contaminant removal, and biochemical component (lipid, carbohydrate, and protein) accumulation potential of Chlorella vulgaris, Scenedesmus obliquus, and Oocystis minuta cells in wastewater supplemented with different concentrations of sulfate, nitrate, and phosphate. The results show maximum biomass productivity of 33, 19, and 98 mg dw/L/d for C. vulgaris, S. obliquus, and O. minuta, respectively. Phosphate removal (more than 90%) was highest in the culture with O. minuta; about 93% nitrate was removed by C. vulgaris, and the highest sulfate removal of 36% was observed in the culture with S. obliquus. The biochemical composition of the microalgae cells is in the ranges of 22–65% carbohydrate, 19–38% protein, and 8–17% lipid. This indicates that carbohydrate and protein are preferentially accumulated as compared to lipids under the growth conditions investigated for each of the microalgae strains.

scholarly journals RETRACTED ARTICLE: Increased biomass productivity in green algae by tuning non-photochemical quenching

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Silvia Berteotti ◽  
Matteo Ballottari ◽  
Roberto Bassi
Keyword(s):  
Heat Dissipation ◽  
Biomass Productivity ◽  
Growth Conditions ◽  
Complex Stress ◽  
Biofuel Production ◽  
Photochemical Quenching ◽  
Protein Accumulation ◽  
Potential Productivity ◽  
Wide Range ◽  
Non Photochemical Quenching

Abstract Photosynthetic microalgae have a high potential for the production of biofuels and highly valued metabolites. However, their current industrial exploitation is limited by a productivity in photobioreactors that is low compared to potential productivity. The high cell density and pigment content of the surface layers of photosynthetic microalgae result in absorption of excess photons and energy dissipation through non-photochemical quenching (NPQ). NPQ prevents photoinhibition, but its activation reduces the efficiency of photosynthetic energy conversion. In Chlamydomonas reinhardtii, NPQ is catalyzed by protein subunits encoded by three lhcsr (light harvesting complex stress related) genes. Here, we show that heat dissipation and biomass productivity depends on LHCSR protein accumulation. Indeed, algal strains lacking two lhcsr genes can grow in a wide range of light growth conditions without suffering from photoinhibition and are more productive than wild-type. Thus, the down-regulation of NPQ appears to be a suitable strategy for improving light use efficiency for biomass and biofuel production in microalgae.

scholarly journals Optimizing Chlorella Vulgaris And Anabaena Variabilis Growth Conditions For Use As Biofuel Feedstock

2016 ◽  
Vol 42 (2) ◽  
pp. 191-200
Author(s):  
NJ Tarin ◽  
NM Ali ◽  
AS Chamon ◽  
MN Mondol ◽  
MM Rahman ◽  
...  
Keyword(s):  
Light Intensity ◽  
Chlorella Vulgaris ◽  
Vitamin B1 ◽  
Growth Conditions ◽  
Anabaena Variabilis ◽  
Biofuel Production ◽  
Optimum Growth ◽  
Biofuel Feedstock ◽  
Isolation And Characterization ◽  
Physical And Chemical

Isolation and characterization of Chlorella vulgaris (green alga) and Anabaena variabilis (cyanobacterium) were made from natural and artificial water bodies of Dhaka University and Khulna, Bangladesh from March through December 2014 using modified Chu-10D medium to determine their potential as feedstock for biofuel production. Optimum growth measured as total chlorophyll and optical density under varying physical and chemical environments was determined. The optimum growth for C. vulgaris was obtained at pH 6.5 under light intensity of 110 μE m-2 s-1 and one and a half times the concentration of the Chu-10D. Compared to this, the optimum growth for A. variabilis was obtained at 7.0 pH, 90 μE m-2 s-1 light intensity and normal Chu 10D. Both organisms were grown at 25o C temperature. Aeration of medium showed a significant positive growth for both the isolates. Supplementation of medium with vitamin B1, B6, B7 and B12 would yield higher biomass of C. vulgaris as biofuel feedstock. Vitamins were not required for growing A. variabilis. Asiat. Soc. Bangladesh, Sci. 42(2): 191-200, December 2016

scholarly journals Improvement of lab-scale production of microalgal carbohydrates for biofuel production

2012 ◽  
Vol 5 (1) ◽  
pp. 103-116 ◽  
Author(s):  
Silvia-Juliana Jerez-Mogollón ◽  
Laura-Viviana Rueda-Quiñonez ◽  
Laura-Yulexi Alfonso-Velazco ◽  
Andrés-Fernando Barajas-Solano ◽  
Crisóstomo Barajas-Ferreira ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Biomass Productivity ◽  
Biofuel Production ◽  
Scale Production ◽  
Initial Concentration ◽  
The Third ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
Do So

This work studied the improvement of biomass and carbohydrate (glucose and xylose) lab–scale productivity in Chlorella vulgaris UTEX 1803 through the use of the carbon/nitrogen ratio. In order to do so, mixotrophic cultures were made by the modification of initial concentration of CH3COONa (5, 10 and 20 mM) and NaNO3 (0.97, 1.94 and 2.94 mM). All treatments were maintained at 23 ± 1ºC, with light/dark cycles of 12h : 12h for 5 days.It was found that in addition to the carbon/nitrogen ratio, time also influences the concentration of biomass and carbohydrates. The treatment containing 10 mM acetate: 1.94 mM nitrate, reached a concentration of 0.79 g/L of biomass, 76.9 μg/mL of xylose and 73.7 μg/mL of glucose in the fifth day. However, the treatmentcontaining 20 mM acetate: 0.97 mM nitrate produced 1.04 g/L of biomass, 78.9 μg/mL of xylose and 77.2 μg/mL of glucose in the third day, while in the same day the treatment containing 0 mM acetate: 2.94 mM nitrate, produced 0.55 g/L of biomass, 40.2 μg/mL of xylose and 31.3 μg/mL of glucose.The use of carbon/nitrogen ratios improved biomass productivity (from 0.55 to 1.04 g/L) as well as xylose (from 40.2 to 78.9 μg/mL) and glucose (from 31.3 to 77.2 μg/mL) concentration, representing an improvement of up to two times the production of both biomass and carbohydrates in only 3 days of culture.

scholarly journals Growth and biochemical composition of Chlorella vulgaris in different growth media

2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Physiological Response ◽  
Low Cost ◽  
Cost Effective ◽  
Growth Conditions ◽  
Growth Media ◽  
Continuous Cultures ◽  
The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

Influence of mixing and shear stress on Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii

2012 ◽  
Vol 25 (2) ◽  
pp. 485-495 ◽  
Author(s):  
Marco Leupold ◽  
Stefan Hindersin ◽  
Giselher Gust ◽  
Martin Kerner ◽  
Dieter Hanelt
Keyword(s):  
Shear Stress ◽  
Chlamydomonas Reinhardtii ◽  
Chlorella Vulgaris ◽  
Scenedesmus Obliquus

scholarly journals The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 463 ◽  
Author(s):  
Ali Nawaz Kumbhar ◽  
Meilin He ◽  
Abdul Razzaque Rajper ◽  
Khalil Ahmed Memon ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Lipid Content ◽  
Fossil Fuels ◽  
Fossil Fuel ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Biofuel Production ◽  
Promising Strategy ◽  
Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

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