scholarly journals Forsterite-hydroxyapatite composite scaffolds with photothermal antibacterial activity for bone repair

2021 ◽  
Vol 10 (5) ◽  
pp. 1095-1106
Author(s):  
Weiye Liu ◽  
Rongtai Zuo ◽  
Tanglong Zhu ◽  
Min Zhu ◽  
Shichang Zhao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Hydrothermal Treatment ◽  
Bone Repair ◽  
Ph Value ◽  
Bone Defects ◽  
Photothermal Effect ◽  
Bone Mesenchymal Stem Cells ◽  
Hydrothermal Temperature ◽  
Antibacterial Ability ◽  
Ha Coating

AbstractBone engineering scaffolds with antibacterial activity satisfy the repair of bacterial infected bone defects, which is an expected issue in clinical. In this work, 3D-printed polymer-derived forsterite scaffolds were proposed to be deposited with hydroxyapatite (HA) coating via a hydrothermal treatment, achieving the functions of photothermal-induced antibacterial ability and bioactivity. The results showed that polymer-derived forsterite scaffolds possessed the photothermal antibacterial ability to inhibit Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in vitro, owing to the photothermal effect of free carbon embedded in the scaffolds. The morphology of HA coating on forsterite scaffolds could be controlled through changing the hydrothermal temperature and the pH value of the reaction solution during hydrothermal treatment. Furthermore, HA coating did not influence the mechanical strength and photothermal effect of the scaffolds, but facilitated the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) on scaffolds. Hence, the HA-deposited forsterite scaffolds would be greatly promising for repairing bacterial infected bone defects.

Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

2019 ◽  
Vol 70 (6) ◽  
pp. 1983-1987
Author(s):  
Cristian Trambitas ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Dorin Constantin Dorobantu ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Specific Protocol ◽  
Repair Mechanisms ◽  
Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

scholarly journals Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2302
Author(s):  
Qingwen Yu ◽  
Zhiyuan Meng ◽  
Yichao Liu ◽  
Zehao Li ◽  
Xing Sun ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Regeneration ◽  
Water Uptake ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Ph Value ◽  
Chitosan Nanoparticles ◽  
Vitro Assay ◽  
Long Term Treatment

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

scholarly journals Hydrothermal Synthesis of Various Shape-Controlled Europium Hydroxides

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 529
Author(s):  
Hongjuan Zheng ◽  
Kongjun Zhu ◽  
Ayumu Onda ◽  
Kazumichi Yanagisawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ph Value ◽  
Hydrothermal Temperature ◽  
X Ray ◽  
Hydrothermal Reactions ◽  
Starting Solution ◽  
Initial Ph ◽  
Shape Controlled ◽  
Scanning Electron

Eu(OH)3 with various shape-controlled morphologies and size, such as plate, rod, tube, prism and nanoparticles was successfully synthesized through simple hydrothermal reactions. The products were characterized by XRD (X-Ray Powder Diffraction), FE-SEM (Field Emission- Scanning Electron Microscopy) and TG (Thermogravimetry). The influence of the initial pH value of the starting solution and reaction temperature on the crystalline phase and morphology of the hydrothermal products was investigated. A possible formation process to control morphologies and size of europium products by changing the hydrothermal temperature and initial pH value of the starting solution was proposed.

scholarly journals Biomaterial design strategies to address obstacles in craniomaxillofacial bone repair

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17809-17827
Author(s):  
Marley J. Dewey ◽  
Brendan A. C. Harley
Keyword(s):  
Bone Repair ◽  
Bone Defects ◽  
Design Strategies

There exist many challenges in the process of regenerating craniomaxillofacial bone defects, thus biomaterials must be designed to overcome these.

Hydrothermal Growth of Nano-Hydroxyapatite Coating Formed on Fluorinated Magnesium Alloy and Its Corrosion Behaviour

2021 ◽  
Vol 13 (1) ◽  
pp. 10-19
Author(s):  
Chun-Yan Zhang ◽  
Hao-Lan Fang ◽  
Xin-Peng Liu ◽  
Fan-Cheng Meng ◽  
Zhong-Qing Tian ◽  
...  
Keyword(s):  
Magnesium Alloy ◽  
Calcium Phosphate ◽  
Hydrothermal Treatment ◽  
Treatment Time ◽  
Electrochemical Impedance ◽  
Hydrothermal Growth ◽  
Crystal Phase ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Ha Coating

In order to explore the hydrothermal growth mechanism of hydroxyapatite (HA) coating on fluorinated magnesium alloy, the changes of morphology, composition and crystal phase of the calcium phosphate coating during the hydrothermal treatment were studied. And the change of electrochemical impedance spectroscopy (EIS) of the coating specimen of different hydrothermal treatment time was discussed to further understanding the change of the coating structure. The results demonstrated that calcium phosphate could rapidly nucleate on fluorinated AZ31 magnesium alloy. The crystal phase of calcium phosphate coating was mainly octacalcium phosphate (OCP) at the early stage of hydrothermal treatment. Then the content of OCP decreased and the content of HA increased with hydrothermal time. The coating consisted of only HA after hydrothermal treatment for about 4h. The HA coating composed of rod-like crystals exhibited an obvious double layer structure. The rod-like crystals of inner layer arranged into dense bundles and the rod-like crystals of outer layer arranged into loose chrysanthemum-like clusters. Fluoride conversion layer acted as an intermediate transition layer to connect magnesium alloy and HA coating into a whole. The results of immersion test in simulated body fluid demonstrated that HA crystals dissolved slowly. No peeling occurred of HA coating during the 12 days' immersion. Pitting corrosion was still the mainly corrosion mode of magnesium alloy substrate due to the electrolyte infiltration during the immersion.

scholarly journals Evaluation of the pH, calcium release and antibacterial activity of MTA Fillapex

2013 ◽  
Vol 42 (5) ◽  
pp. 330-335 ◽  
Author(s):  
Milton Carlos Kuga ◽  
Gisele Faria ◽  
Paulo Henrique Weckwerth ◽  
Marco Antonio Hungaro Duarte ◽  
Edson Alves De Campos ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Calcium Release ◽  
Ph Value ◽  
Diffusion Test ◽  
Evaluation Period ◽  
Ph Values ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
And Staphylococcus Aureus

OBJECTIVE: This study evaluated, in several analysis periods, pH and calcium release and antibacterial activity provided by MTA Fillapex sealer compared to Sealapex and AH Plus sealers. MATERIAL AND METHOD: Polyethylene tubes were filled with a sealer and immersed in distilled water. After 24 hours, 14 and 28 days, pH and calcium release by endodontic sealers were evaluated directly in water which the tubes were stored. Sealers antibacterial activity was evaluated against Enterococcus faecalis and Staphylococcus aureus by means of agar diffusion test. All data were submitted to ANOVA and Tukey tests (α=0.05). RESULT: In all periods evaluated, Sealapex had the highest pH value (p<0.05) in comparison to other sealers and MTA Fillapex provided higher pH values than AH Plus (p<0.05). In 14-days period, MTA Fillapex had greater calcium release value than Sealapex (p<0.05). In 28-days period, Sealapex provided higher calcium release than MTA Fillapex (p<0.05). In all periods, AH Plus provided lower calcium release than other sealers (p<0.05). In relation to E. faecalis, there were no differences among the sealers, in relation to antibacterial activity (p>0.05). In relation to S. aureus, Sealapex presented better antibacterial effectiveness than the MTA Fillapex and AH Plus (p<0.05), which were similar each other (p>0.05). CONCLUSION: In final evaluation period, pH values and calcium release provided by MTA Fillapex were lower than provided by Sealapex and higher than provided by AH Plus. The MTA Fillapex antimicrobial action was similar to other endodontic sealers.

Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

2017 ◽  
Vol 204 (3-4) ◽  
pp. 137-149 ◽  
Author(s):  
Vanessa B. Andrade ◽  
Marcos A. Sá ◽  
Renato M. Mendes ◽  
Paulo A. Martins-Júnior ◽  
Gerluza A.B. Silva ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Repair ◽  
Bone Matrix ◽  
Sodium Hyaluronate ◽  
Repair Process ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Bone Trabeculae ◽  
Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
SAMSUL RIZAL ◽  
Suharyono Suharyono ◽  
Fibra Nuariny ◽  
Julfi Restu Amelia
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Low Temperature ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Ph Value ◽  
Low Temperature Storage ◽  
The Stability ◽  
Temperature Storage

Abstract. Rizal S, Suharyono, Nurainy F, Amela JR. 2020. The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks. Biodiversitas 21: 3826-3831. Synbiotic drinks from green grass jelly have shown antibacterial activity against pathogenic bacteria. These are usually stored at low temperatures to maintain their characteristics. The aim of this study was to determine the effect of storage at low temperature of 10°C on the viability of lactic acid bacteria (Lactobacillus casei) and the stability of the antibacterial activity in synbiotic drinks made of green grass jelly. Antibacterial activity of green grass jelly synbiotic drink was conducted against pathogenic bacteria (Staphylococcus aureus, Salmonella sp., Bacillus cereus, and Escherichia coli). The products were stored for 28 days at 10°C temperature. Observations on the antibacterial activity, pH value, total acid, and total lactic acid bacteria were carried out every 7 days. Antibacterial activity was evaluated using agar well diffusion method. The results showed that storage at low temperature (10 ± 2°C) for 28 days decreased the antibacterial activity and pH value but sharply increased total lactic acid bacteria (at 0 to 7 days of storage) in green grass jelly synbiotic drinks. Salmonella sp. showed the highest inhibition caused by the antibacterial agents in green grass jelly synbiotic drinks while the lowest inhibition was found on Staphylococcus aureus. During storage at low temperature, green grass jelly synbiotic drinks had a total of lactic acid bacteria that ranged from 9.51 to 10.10 (Log CFU/mL) or equal to 3.24x109-1.26x1010 CFU/mL; a total of lactic acid that ranged from 0.48% to 0.87%; and pH values that ranged from 3.78 to 4.08.

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