scholarly journals Leukocytospermia in late adolescents: possible clinical interpretations

2020 ◽  
Author(s):  
S. La Vignera ◽  
R. Cannarella ◽  
A. Aversa ◽  
R. Rago ◽  
R. A. Condorelli ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Cross Sectional Study ◽  
Cell Number ◽  
Late Adolescents ◽  
Cross Sectional ◽  
Unprotected Sexual Intercourse ◽  
Sperm Analysis ◽  
Male Adolescents ◽  
Risky Lifestyles

Abstract Background No data are currently available on the implication of amicrobial leukocytospermia in male adolescents. Therefore, the primary aim of this study was to evaluate the prevalence of amicrobial leukocytospermia among non-smoker late adolescents who were exposed to other risky lifestyles for the andrological health. The main andrological clinical features of adolescents with leukocytospermia were also reported. Methods This is a cross-sectional study carried out in 80 boys. Each adolescent underwent a physical examination, and to the assessment of sperm conventional parameters, seminal leukocytes concentration and immature germ cell evaluation. A possible correlation between seminal leukocytes and immature germ cells and testicular volume (TV) was tested. Results The adolescents enrolled in this study had 18.0 ± 0.4 (range 18.1–18.9) years. Unprotected sexual intercourse was referred by 38% of them. Sexual dysfunctions were found in 25% and isolated hypoactive sexual desire in 12.5% of boys. Low TV and penile length in flaccidity were found in 44% and 30% of them, respectively. Only 41% had normozoospermia at the sperm analysis, whereas 19% had isolated oligozoospermia, 15% oligo-asthenozoospermia, and 25% oligo-astheno-teratozoospermia. Leukocytospermia occurred in 25% (20 out of 80) of adolescents. No seminal infection was detected in 19% (15 out of 80) of them. Adolescents with leukocytospermia had lower progressive sperm motility, percentage of normal forms, TV, and a higher percentage of immature germ cells compared to those without leukocytospermia. Semen leukocyte concentration correlated negatively with TV and positively with the percentage of immature germ cells in the ejaculate. Conclusion Leukocytospermia, increased immature germ cell number, and low TV identify a distinct phenotype suggestive of testicular tubulopathy. Primary prevention of male infertility and the counselling for andrological risky lifestyles is mandatory and should be started as early as possible.

scholarly journals The causes of infertility in women presenting to gynaecology clinics in Harare, Zimbabwe; a cross sectional study

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mugove G. Madziyire ◽  
Thulani L. Magwali ◽  
Vasco Chikwasha ◽  
Tinovimba Mhlanga
Keyword(s):  
Assisted Reproduction ◽  
Cross Sectional Study ◽  
Male Factor ◽  
Cross Sectional ◽  
Unprotected Sexual Intercourse ◽  
Assisted Reproduction Techniques ◽  
Public Facilities ◽  
Tubal Blockage ◽  
Private And Public

Abstract Background Infertility affects 48.5 million couples globally. It is defined clinically as failure to conceive after 12 months or more of regular unprotected sexual intercourse. The contribution of various aetiological factors to infertility differs per population. The causes of infertility have not been assessed in Zimbabwe. Our objectives were to determine the reproductive characteristics, causes and outcomes of women presenting for infertility care. Methods A retrospective and prospective study of women who had not conceived within a year of having unprotected intercourse presenting in private and public facilities in Harare was done. A diagnosis was made based on the history, examination and results whenever these were deemed sufficient. Data was analysed using STATA SE/15. A total of 216 women were recruited. Results Of the 216 women recruited, two thirds (144) of them had primary infertility. The overall period of infertility ranged from 1 to 21 years with an average of 5.6 ± 4.7 years whilst 98 (45.4%) of the couples had experienced 2–4 years of infertility and 94 (43.5%) had experience 5 or more years of infertility. About 1 in 5 of the women had irregular menstrual cycles with 10 of them having experienced amenorrhoea of at least 1 year. Almost half of the participants (49%) were overweight or obese. The most common cause for infertility was ‘unexplained’ in 22% of the women followed by tubal blockage in 20%, male factor in 19% and anovulation in 16%. Of the 49 (22.7%) women who conceived 21(9.7%) had a live birth while 23 (10.7%) had an ongoing pregnancy at the end of follow up. Thirty-seven (17.1%) had Assisted Reproduction Techniques (ART) in the form of Invitro-fertilisation/Intracytoplasmic Sperm Injection (IVF/ICSI) or Intra-Uterine Insemination (IUI). Assisted Reproduction was significantly associated with conception. Conclusion Most women present when chances of natural spontaneous conception are considerably reduced. This study shows an almost equal contribution between tubal blockage, male factor and unexplained infertility. Almost half of the causes are female factors constituted by tubal blockage, anovulation and a mixture of the two. Improved access to ART will result in improved pregnancy rates. Programs should target comprehensive assessment of both partners and offer ART.

scholarly journals Müllerian Inhibiting Substance Is Required for Germ Cell Proliferation during Early Gonadal Differentiation in Medaka (Oryzias latipes)

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
Eri Shiraishi ◽  
Norifumi Yoshinaga ◽  
Takeshi Miura ◽  
Hayato Yokoi ◽  
Yuko Wakamatsu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Sex Differentiation ◽  
Somatic Cells ◽  
Oryzias Latipes ◽  
Cell Number ◽  
Gonadal Differentiation ◽  
Loss Of Function ◽  
Germ Cell Proliferation

Müllerian inhibiting substance (MIS) is a glycoprotein belonging to the TGF-β superfamily. In mammals, MIS is responsible for the regression of Müllerian ducts in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fish, which have no Müllerian ducts, has yet to be clarified. In the present study, we examined the expression pattern of mis and mis type 2 receptor (misr2) mRNAs and the function of MIS signaling in early gonadal differentiation in medaka (teleost, Oryzias latipes). In situ hybridization showed that both mis and misr2 mRNAs were expressed in the somatic cells surrounding the germ cells of both sexes during early sex differentiation. Loss-of-function of either MIS or MIS type II receptor (MISRII) in medaka resulted in suppression of germ cell proliferation during sex differentiation. These results were supported by cell proliferation assay using 5-bromo-2′-deoxyuridine labeling analysis. Treatment of tissue fragments containing germ cells with recombinant eel MIS significantly induced germ cell proliferation in both sexes compared with the untreated control. On the other hand, culture of tissue fragments from the MIS- or MISRII-defective embryos inhibited proliferation of germ cells in both sexes. Moreover, treatment with recombinant eel MIS in the MIS-defective embryos dose-dependently increased germ cell number in both sexes, whereas in the MISRII-defective embryos, it did not permit proliferation of germ cells. These results suggest that in medaka, MIS indirectly stimulates germ cell proliferation through MISRII, expressed in the somatic cells immediately after they reach the gonadal primordium.

GENDER AND RURAL–URBAN DIFFERENCES IN THE NUTRITIONAL STATUS OF IN-SCHOOL ADOLESCENTS IN SOUTH-WESTERN NIGERIA

2010 ◽  
Vol 42 (5) ◽  
pp. 653-676 ◽  
Author(s):  
OLAYINKA O. OMIGBODUN ◽  
KOFOWOROLA I. ADEDIRAN ◽  
JOSHUA O. AKINYEMI ◽  
AKINYINKA O. OMIGBODUN ◽  
BABATUNDE O. ADEDOKUN ◽  
...  
Keyword(s):  
Private Schools ◽  
Rural Schools ◽  
Private School ◽  
Urban Areas ◽  
Cross Sectional Study ◽  
School Type ◽  
Cross Sectional ◽  
Male Adolescents ◽  
Demographic Correlates ◽  
Low Bmi

SummaryThis study assessed gender and rural/urban differences in height and weight, and the prevalence of stunting, underweight and overweight of school-going adolescents in south-west Nigeria, using 2007 WHO reference values for comparison. The influence of sexual maturity and the socio-demographic correlates of growth performance were also examined. In this cross-sectional study, 924 male (51.4%) and 875 female (48.6%) students (1799 in total) aged 10–19 years from eighteen schools in Ibadan (five rural, nine urban public and four urban private) were interviewed and examined. Although males were significantly taller than females (p<0.05), stunting was more pronounced for males, who were 7.5 cm shorter than the 2007 WHO reference, compared with females who were 3.5 cm shorter. Body mass index (BMI) for girls was also greater than for boys (p<0.05). Rural adolescents had lower heights and BMIs compared with those in urban areas. The mean height of male adolescents in rural schools fell below 2 SDs of the 2007 WHO reference between 14 and 17 years, while heights of males and females in private schools were similar to the median 2007 WHO standard. Low height-for-age was observed in 282 adolescents (15.7%), which, after multivariate analysis, was significantly associated with school type, gender, number of mother's children and puberty onset. Adolescents in rural schools were much more likely to be stunted than those in urban private schools (AOR 13.1; 95% CI 5.2–33.2) and males were three times more likely to be stunted compared with females (AOR 3.3; 95% CI 2.4–1.4). Low BMI-for-age was observed in 240 adolescents (18.9%), with correlates similar to stunting. Adolescents at the pre-puberty stage were twice as likely to have low BMI-for-age (OR 2.0; 95% CI 1.6–2.5) than those with signs of puberty. There were 2.3% overweight adolescents, who were significantly more likely to be female, in private school and post-pubertal. Innovative interventions for Nigerian adolescents, especially rural inhabitants and males, are needed to reduce the prevalence of stunting and underweight.

scholarly journals Knowledge and attitude towards emergency contraception in females of urban area of Lucknow, Uttar Pradesh, India

2016 ◽  
Vol 6 (1) ◽  
pp. 210 ◽  
Author(s):  
Malvika Mishra ◽  
Shiv Shanker Tripathi ◽  
Manish Kumar Verma
Keyword(s):  
Health Education ◽  
Emergency Contraception ◽  
Unplanned Pregnancy ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Health Awareness ◽  
Cross Sectional ◽  
Negative Attitudes ◽  
Unprotected Sexual Intercourse ◽  
Knowledge And Attitude

Background: Emergency contraception are birth control measures that, if taken after unprotected sexual intercourse, contraceptive failure, incorrect use of contraceptives or in cases of sexual assault may prevent pregnancy. The aim of the study was to assess the knowledge and attitude of female living in urban part of Lucknow district from September 2015 to August 2016.Methods: This was a descriptive, cross-sectional study conducted at RML Hospital, Lucknow from September 2015 to August 2016. A cross sectional study design was conducted and Participants were selected using simple random sampling method. Data was collected by using self-administered questionnaires. Data processing and analysis: The collected data was entered into a computer and analyzed using statistical package of social science version 20.0.Results: In this study it was found that, Out of 385 subjects 54% (209) new the correct time interval for EC to be effective and 91% (350) new that this is for prevention of unplanned pregnancy. Out of 385 subjects 77% (345) reported that they had heard the word EC from different sources, however 23% (90) reported that they never heard about that it means that still there is a scope of health awareness activities and health education to improve the knowledge of EC. Different attitudes of females towards emergency contraception some different pair of questions was asked. Out of 385 subjects 85% (329) were reported that they have positive attitudes in terms of benefits of EC to reduce the risk of unplanned pregnancy. A few number of subjects i.e. 12% (48) reported negative attitudes towards EC where as 3 % (8) did not respond. The more than half subjects i.e. 68% (260) reported that they use EC even if their partners disagreed with them where as 11% (41). Cultural belief was found as an barrier in having negative attitudes towards EC. The Religion, Occupation and educational status affect knowledge and attitudes towards EC.Conclusions: The results show that there is a scope of health awareness activities and health education to improve the knowledge and attitude towards EC.

scholarly journals Prevalence of nutritional deficiencies among school going adolescents of Vadodara, Gujarat, India: a cross sectional study

2019 ◽  
Vol 6 (6) ◽  
pp. 1767
Author(s):  
Ashish Shamjibhai Bhalsod ◽  
Nisarg N. Dave ◽  
Nilesh Thakor
Keyword(s):  
Vitamin A Deficiency ◽  
Protein Energy Malnutrition ◽  
Cross Sectional Study ◽  
Nutritional Deficiencies ◽  
Growth Spurt ◽  
Cross Sectional ◽  
Male Adolescents ◽  
Protein Energy ◽  
Complex Deficiency ◽  
Fatty Acid Deficiency

Background: Adolescent constitutes over 23% of the population in India. Critical development occurs during adolescence period. Growth spurt and increase in physical activity during adolescent period increases the nutrition and health needs of the adolescent. Objective was to study prevalence of nutritional deficiencies among school going adolescents of Vadodara city, Gujarat.Methods: The study was carried during period from September 2018 to August 2019. After taking the permission of principals of 3 schools and consent of the parents of adolescents, 511 adolescents from 3 schools of Vadodara city were examined for signs of various nutritional deficiencies. The data was collected by predesign, pretested proforma and analyzed using SPSS 17.0 (Trial Version).Results: Out of total 511 adolescents 253(49.5%) were female. Mean age of the study adolescents was 15.6±1.81 years. Maximum numbers of the adolescents were in the age group of 10-14 years (60.3%). Mean age of female and male adolescents was 15.2±1.71 years and 15.6±2.01 years respectively. The study revealed that vitamin A deficiency was present in 38(7.4%) adolescents. Vitamin B complex deficiency signs were seen in 112(21.9%) adolescents. Vitamin C deficiency signs were seen in 43(8.4%) adolescents. Protein Energy Malnutrition was observed in 52(10.1%) adolescents. Essential fatty acid deficiency was observed in 56(10.9%) adolescents.Conclusions: High prevalence of nutritional deficiencies among these adolescents needs great attention and health education.

scholarly journals A cross-sectional study on extensive gaming in adolescents

2021 ◽  
Author(s):  
Frida André ◽  
Emma Claesdotter-Knutsson ◽  
Maria Fridh ◽  
Carl Delfin ◽  
Anders Håkansson ◽  
...  
Keyword(s):  
School Health ◽  
Subjective Health ◽  
Cross Sectional Study ◽  
Physical Symptoms ◽  
Child And Adolescent Psychiatry ◽  
Poor Sleep ◽  
Cross Sectional ◽  
Male And Female ◽  
Male Adolescents ◽  
9Th Grade

Background: Extensive gaming and the consequences thereof is frequently reported from child and adolescent psychiatry and school health care. The behavior is associated with compulsion, psychiatric and physical symptoms, impaired cognitive development and poorer school performance. This phenomenon has been described as an emergent health issue for men and little is known about its potential gender-specific characteristics. The aim of this study was to explore extensive gaming among male and female adolescents and to investigate whether the frequency of often feeling low, often feeling anxious, self-reported ADHD, self-reported ASD, being satisfied with one’s own general health, poor sleep, loneliness, and having tried smoking, alcohol, and/or other substances differed among those with and without extensive gaming.Design and Methods: This study was based on data collected through a public health survey distributed in 2016 to pupils in 9th grade of primary school and in second grade of secondary school, including a total of 13498 participants. The association between extensive gaming and different factors was estimated among male and female respondents separately.Results: Roughly 30% of the male and 5% of the female respondents were categorized as extensive gamers. Extensive gaming was associated with a higher prevalence of poor sleep and a lower prevalence of being satisfied with one’s own health among boys and (to a higher degree) among girls.Conclusions: Altogether, our results contribute to the impression that extensive gaming is more heavily related to subjective health complaints among female than male adolescents.

scholarly journals Prevalence and Knowledge on Obesity Among School Going Adolescents of Kaski, Nepal

2019 ◽  
Vol 38 (2) ◽  
pp. 63-68
Author(s):  
Apsara Pandey ◽  
Sony Sapkota
Keyword(s):  
Preventive Measure ◽  
Cross Sectional Study ◽  
Calorie Intake ◽  
Chi Square ◽  
Cross Sectional ◽  
Significance Level ◽  
Male Adolescents ◽  
Adolescent Students ◽  
Inadequate Knowledge ◽  
Prevalence Of Obesity

Introduction: Childhood obesity has now been recognized as a global health problem because of its devastating consequences and prevalence at uncontrollable rate worldwide. The objective of this study was to determine prevalence of obesity and find out the knowledge on obesity among school going adolescents. Material and Methods: This descriptive cross-sectional study was conducted among adolescents of two private schools of Kaski district. Altogether 120 adolescent students were selected using census method. Height and weight were measured with standard scale and BMI was calculated. Self-administered structured questionnaire was used to assess knowledge. Descriptive statistics and Chi-square test at 0.05 significance level was used to analyze data. Results: The mean age of adolescents was 15.1 years and 50% of them were male. The prevalence of obesity among adolescents was 3.3%. Seventy five percent of the adolescents had knowledge regarding meaning of obesity and 59.2% had mentioned high calorie intake as a risk factor of obesity. Likewise, 52.5% adolescents stated Diabetes mellitus as consequence of obesity. Ninety eight percent mentioned regular exercise as a preventive measure of obesity. More than three fourth (78.3%) of the adolescents had inadequate knowledge regarding obesity. Male adolescents had significantly higher knowledge than female adolescents (p=0.00). Conclusion: Even though very few adolescents had obesity, there were adolescents with overweight which were at more risk for getting obesity. Adolescents have inadequate knowledge regarding obesity.

scholarly journals Germ cell fate and seminiferous tubule development in bovine testis xenografts

Reproduction ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 923-929 ◽  
Author(s):  
Rahul Rathi ◽  
Ali Honaramooz ◽  
Wenxian Zeng ◽  
Stefan Schlatt ◽  
Ina Dobrinski
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Germ Cells ◽  
Cell Number ◽  
Sperm Production ◽  
Continuous Increase ◽  
Control Tissue ◽  
Low Efficiency ◽  
Pachytene Spermatocytes ◽  
Testis Tissue

Spermatogenesis can occur in testis tissue from immature bulls ectopically grafted into mouse hosts; however, efficiency of sperm production is lower than in other donor species. To elucidate a possible mechanism for the impaired spermatogenesis in bovine testis xenografts, germ cell fate and xenograft development were investigated at different time points and compared with testis tissue from age-matched calves as controls. Histologically, an initial decrease in germ cell number was noticed in xenografts recovered up to 2 months post-grafting without an increase in germ cell apoptosis. From 2 months onward, the number of germ cells increased. In contrast, a continuous increase in germ cell number was seen in control tissue. Pachytene spermatocytes were observed in some grafts before 4 months, whereas in the control tissue they were not present until 5 months of age. Beyond 4 months post-grafting spermatogenesis appeared to be arrested at the pachytene spermatocyte stage in most grafts. Elongated spermatids were observed between 6 and 8 months post-grafting, similar to the controls, albeit in much lower numbers. Lumen formation started earlier in grafts compared with controls and by 6 months post-grafting tubules with extensively dilated lumen were observed. A donor effect on efficiency of spermatogenesis was also observed. These results indicate that the low efficiency of sperm production in bovine xenografts is due to an initial deficit of germ cells and impaired meiotic and post-meiotic differentiation. The characterization of spermatogenic efficiency will provide the basis to understand the control of spermatogenesis in testis grafts.

scholarly journals A novel pattern of germ cell divisions in the production of hymenopteran insect eggs

2020 ◽  
Vol 16 (5) ◽  
pp. 20200137
Author(s):  
Katherine J. Eastin ◽  
Austin P. Huang ◽  
Patrick M. Ferree
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Radial Direction ◽  
Fruit Fly ◽  
Cell Number ◽  
Nurse Cells ◽  
Insect Eggs ◽  
Ring Canals ◽  
Cytoplasmic Bridges ◽  
Higher Eukaryotes

Egg development is a defining process of reproduction in higher eukaryotes. In the fruit fly, Drosophila melanogaster , this process begins with four mitotic divisions starting from a single germ cell, producing a cyst of 16 cystocytes; one of these cells will become the oocyte and the others supporting nurse cells. These mitotic divisions are exceptional because cytokinesis is incomplete, resulting in the formation of cytoplasmic bridges known as ring canals that interconnect the cystocytes. This organization allows all cystocytes to divide synchronously during each mitotic round, resulting in a final, power-of-2 number of germ cells. Given that numerous insects obey this power-of-2 rule, we investigated if strict cell doubling is a universal, underlying cause. Using confocal microscopy, we found striking departures from this paradigm in three different power-of-2 insects belonging to the Apocrita suborder (ants, bees and wasps). In these insects, the earliest-formed cystocytes cease to divide during the latter mitotic cycles while their descendants undergo further division, thereby producing a ‘radial’ direction of division activity. Such cystocyte division patterns that depart from strict cell doubling may be ‘fine-tuned’ in order to maintain a final, power-of-2 germ cell number.

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