3D spiral channels combined with flexible micro-sieve for high-throughput rare tumor cell enrichment and assay from clinical pleural effusion samples

2022 ◽  
Author(s):  
Jie Cheng ◽  
Lina Zhang ◽  
Yiran Zhang ◽  
Yifei Ye ◽  
Wenjie Zhao ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Tumor Cell ◽  
High Throughput ◽  
Rare Tumor ◽  
Cell Enrichment

A high-throughput liquid biopsy for rapid rare cell separation from large-volume samples

Lab on a Chip ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 68-78 ◽  
Author(s):  
Yaoping Liu ◽  
Tingyu Li ◽  
Mingxin Xu ◽  
Wei Zhang ◽  
Yan Xiong ◽  
...  
Keyword(s):  
Tumor Cell ◽  
High Throughput ◽  
Cancer Detection ◽  
Large Volume ◽  
Liquid Biopsy ◽  
Cell Separation ◽  
Body Fluids ◽  
Rare Tumor

The developed high-throughput liquid biopsy platform for rare tumor cell separation from body fluids has shown enormous promise in cancer detection and prognosis monitoring.

A single‐view field filter device for rare tumor cell filtration and enumeration

2020 ◽  
Vol 41 (23) ◽  
pp. 2000-2006
Author(s):  
Yunlin Quan ◽  
Ke Chen ◽  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cell ◽  
Rare Tumor ◽  
Cell Filtration ◽  
Single View

scholarly journals High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients

2017 ◽  
Vol 114 (10) ◽  
pp. 2544-2549 ◽  
Author(s):  
Yin Tang ◽  
Zhuo Wang ◽  
Ziming Li ◽  
Jungwoo Kim ◽  
Yuliang Deng ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Pleural Effusion ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Pleural Fluid ◽  
High Throughput ◽  
Targeted Therapies ◽  
Peripheral Blood ◽  
Detection Scheme ◽  
Lung Cancer Patients

Malignant pleural effusion (MPE), the presence of malignant cells in pleural fluid, is often the first sign of many cancers and occurs in patients with metastatic malignancies. Accurate detection of tumor cells in pleural fluid is crucial because the presence of MPE denotes an advanced stage of disease and directs a switch in clinical managements. Cytology, as a traditional diagnostic tool, has limited sensitivity especially when tumor cells are not abundant, and may be confounded by reactive mesothelial cells in the pleural fluid. We describe a highly sensitive approach for rapid detection of metabolically active tumor cells in MPE via exploiting the altered glucose metabolism of tumor cells relative to benign cells. Metabolically active tumor cells with high glucose uptake, as evaluated by a fluorescent glucose analog (2-NBDG), are identified by high-throughput fluorescence screening within a chip containing 200,000 addressable microwells and collected for malignancy confirmation via single-cell sequencing. We demonstrate the utility of this approach through analyzing MPE from a cohort of lung cancer patients. Most candidate tumor cells identified are confirmed to harbor the same driver oncogenes as their primary lesions. In some patients, emergence of secondary mutations that mediate acquired resistance to ongoing targeted therapies is also detected before resistance is manifested in the clinical imaging. The detection scheme can be extended to analyze peripheral blood samples. Our approach may serve as a valuable complement to cytology in MPE diagnosis, helping identify the driver oncogenes and resistance-leading mutations for targeted therapies.

ClearCell FX: A microfluidic system for label-free circulating tumor cell enrichment.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e22023-e22023 ◽  
Author(s):  
Andrew Wu ◽  
Ali Asgar Bhagat ◽  
Man Chun Leong ◽  
Chwee Teck Lim
Keyword(s):  
Tumor Cell ◽  
Circulating Tumor Cell ◽  
Microfluidic System ◽  
Label Free ◽  
Cell Enrichment

High-throughput live-cell imaging reveals differential inhibition of tumor cell proliferation by human fibroblasts

2010 ◽  
Vol 128 (12) ◽  
pp. 2793-2802 ◽  
Author(s):  
Emilie Flaberg ◽  
Laszlo Markasz ◽  
Gabor Petranyi ◽  
Gyorgy Stuber ◽  
Ferenc Dicső ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
High Throughput ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Fibroblasts ◽  
Live Cell ◽  
Tumor Cell Proliferation ◽  
Differential Inhibition

scholarly journals A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering

2017 ◽  
Vol 27 ◽  
pp. 177-178 ◽  
Author(s):  
Gürhan Özkayar ◽  
Yağmur Demircan Yalçın ◽  
Ebru Özgür ◽  
Ufuk Gündüz ◽  
Haluk Külah
Keyword(s):  
High Throughput ◽  
Cell Enrichment

scholarly journals Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation

2020 ◽  
Author(s):  
Suraj Makhija ◽  
David Brown ◽  
Struan Bourke ◽  
Yina Wang ◽  
Shuqin Zhou ◽  
...  
Keyword(s):  
Protein Expression ◽  
High Throughput ◽  
Protein Interaction ◽  
Genome Engineering ◽  
Small Peptide ◽  
Protein Protein Interaction ◽  
Recent Advances ◽  
Cell Enrichment ◽  
Peptide Tags ◽  
Endogenous Proteins

AbstractRecent advances in genome engineering have expanded our capabilities to study proteins in their natural states. In particular, the ease and scalability of knocking-in small peptide tags has enabled high throughput tagging and analysis of endogenous proteins. To improve enrichment capacities and expand the functionality of knock-ins using short tags, we developed the tag-assisted split enzyme complementation (TASEC) approach, which uses two orthogonal small peptide tags and their cognate binders to conditionally drive complementation of a split enzyme upon labeled protein expression. Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited cells through multiplexing.

Sign in / Sign up

Export Citation Format

Share Document