In Vivo uptake of 57Co, 54Mn and 65Zn by peripheral lymphocytes, tumor and various organs of rats bearing Walker 256 carcinosarcoma

1979 ◽  
Vol 15 (11) ◽  
pp. 1365-1370 ◽  
Author(s):  
J. Schuhmacher ◽  
J. Mattern ◽  
N. Volm ◽  
K. Wayss
Keyword(s):  
Walker 256 Carcinosarcoma ◽  
Peripheral Lymphocytes ◽  
Walker 256 ◽  
In Vivo Uptake

Synthese, in vivo- und in vitro-Untersuchungen zur tumorhemmenden Wirkung von cis-Dichloro-dipeptidester-platin(II)-Komplexen / Synthesis, in vivo and in vitro Studies on the Antineoplastic Effect of cis-Dichloro-dipeptide Ester Platinum(II) Complexes

1976 ◽  
Vol 31 (6) ◽  
pp. 832-845 ◽  
Author(s):  
Wolfgang Beck ◽  
Bernhard Purucker ◽  
Michael Girnth ◽  
Helmut Schönenberger ◽  
Horst Seidenberger ◽  
...  
Keyword(s):  
Protein Biosynthesis ◽  
Sarcoma 180 ◽  
Protecting Group ◽  
Yoshida Sarcoma ◽  
Walker 256 Carcinosarcoma ◽  
E Coli ◽  
Antineoplastic Effect ◽  
Walker 256

cis-Dichlorodipeptide esterplatinum complexesCl2Pt(MetGlyOEt),Cl2Pt(EthionylGlyOEt), Cl2Pt(GlyGlyOEt)2 and Cl2Pt(GlySerOEt)2 are prepared from the α-amino acid complexes by peptide synthesis using platinum as an amino protecting group. cis-Cl2Pt(GlyGlyOEt)2 and cis-Cl2Pt(GlySerOEt)2 have been prepared also directly from K2PtCl4 and the dipeptidesters. cis-Cl2Pt(GlyGlyOEt)2 (2 a) and cis-Cl2Pt(NH3)2 (5) lead to a prefered inhibition of the DNA-synthesis of sarcoma 180, Yoshida-sarcoma and Walker-256-carcinosarcoma in vitro; RNA- and protein biosynthesis are influenced to a much lower degree. 2a and 5 cause filamentous growth in Escherichia coli B. The DNA polymerase deficient strain of E. coli, p 3478 pol A-, is more inhibited by 2 a and 5 than the non deficient strain W 3110 pol A+. Tumor growth of di-2-chloro-ethylmethylamine (HN2) resistant sarcoma 180 and of Yoshida sarcoma is weakly inhibited, whereas Walker-256-carcinosarcoma is markedly inhibited; however 2a and 5 show similar inhibition of the same tumor.

scholarly journals MORPHOLOGICAL FEATURES OF DOXORUBICIN-RESISTANT WALKER 256 CARCINOSARCOMA AND RESPONSE OF MAST CELLS

2018 ◽  
Vol 40 (1) ◽  
pp. 42-47
Author(s):  
N V Boroday ◽  
V F Chekhun
Keyword(s):  
Drug Resistance ◽  
Mast Cells ◽  
Staining Intensity ◽  
Morphological Features ◽  
Histamine Content ◽  
Original Strain ◽  
Walker 256 Carcinosarcoma ◽  
Development Of Resistance ◽  
Walker 256

Background: The mechanisms of drug resistance of cancer have not been yet elucidated in details. Recently, the role of mast cells (MCs) in the development of drug resistance has been brought in the limelight. The aim of the study was to examine the morphological features of doxorubicin (DOX)-resistant Walker 256 carcinosarcoma and to assess the response of MCs and histamine content in these cells in relation to the development of resistance to DOX as well as in DOX-resistant tumors. Materials and Methods: The DOX resistance was induced by serial passages of Walker 256 carcinosarcoma in rats in the setting of DOX treatment in vivo. MCs in tumors were detected in the sections by staining with Toluidine Blue O. Histamine content in MCs stained with solution of Water Blue-Orcein was assessed by Astaldi semiquantitative method taking into account different staining intensity. Results: Formation of DOX resistance in the course of serial passages of Walker 256 carcinosarcoma was accompanied by the increase in the number of MCs in tumors and histamine content. Nevertheless, in tumors with phenotype of complete DOX resistance the number of histamine-containing MCs decreased to the same level as in tumors of the original strain that are DOX-sensitive. Conclusion: MCs are involved in formation of DOX resistance in Walker 256 carcinosarcoma.

scholarly journals Effect of release-active anti-interferon-gamma antibodies on the course of the tumor process in in vivo experimental models

2018 ◽  
pp. 132-134
Author(s):  
Т.Г. Разина ◽  
И.А. Эртузун ◽  
Е.Н. Амосова ◽  
С.Г. Крылова ◽  
Е.П. Зуева ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Interferon Gamma ◽  
Conformational Changes ◽  
Hematogenous Metastasis ◽  
Walker 256 Carcinosarcoma ◽  
Melanoma B16 ◽  
Enhanced Production ◽  
Walker 256 ◽  
Stimulation Of

Интерферон-гамма (ИФН) играет важную роль в иммунных механизмах сдерживания опухолевого процесса, однако в определенных условиях может оказывать проопухолевое действие. Релиз-активные антитела (РА АТ) к ИФН изменяют конформацию молекулы этого цитокина, облегчают его связывание с рецептором и усиливают продукцию эндогенного ИФН. Цель настоящего исследования состояла в изучении влияния РА АТ к ИФН на имеющийся опухолевый процесс на моделях меланомы и карциносаркомы. Методика. Было изучено влияние препарата на течение опухолевого процесса в моделях меланомы В-16 у мышей и карциносаркомы Уокера 256 у крыс. Результаты. У мышей с меланомой В-16, чувствительной к ИФН, тестируемый препарат не стимулировал рост и метастазирование опухоли. В модели карциносаркомы Уокера 256 у крыс РА АТ к ИФН также не влияли на размер опухоли, однако значительно ингибировали гематогенное метастазирование. Заключение. В настоящем исследовании не было выявлено стимулирования опухолевого процесса и метастазирования препаратом РА АТ к ИФН. Interferon-gamma (IFN) plays an important role in antitumor immunity; however, in some circumstances, it may also favor tumor immune evasion. Released-active (RA) anti-IFN antibodies (Abs) are able to induce conformational changes in the IFN molecule and to facilitate its receptor binding, which results in enhanced production of this cytokine. The aim of the present study was to evaluate the effect of RA anti-IFN Abs on the tumor growth in models of melanoma and carcinosarcoma. Methods. The ability of anti-IFN RA Abs to influence the tumor growth was evaluated in the models of melanoma B16 in mice and Walker 256 carcinosarcoma in rats. Results. The exposure of mice with IFN-sensitive melanoma B16 to the tested drug did not result in stimulation of tumor growth and metastasis. RA anti-IFN Abs also did not affect the tumor size in the rat model of Walker 256 carcinosarcoma but significantly inhibited the hematogenous metastasis. Conclusion. In the present study, no stimulation of the tumor process and metastasis by RA anti-IFN Abs were found.

In vivo uptake and retention of fluoride after a brief application of TiF4 to dentin

1989 ◽  
Vol 42 (2) ◽  
pp. 65-68 ◽  
Author(s):  
Liv Skartveit ◽  
Anne Bjørrg Tveit ◽  
Björn Klinge ◽  
Bård Tørtdal ◽  
Knut Selvig
Keyword(s):  
In Vivo Uptake

scholarly journals In Vivo Uptake of [3H]Nimodipine into Brain during Cortical Spreading Depression

1997 ◽  
Vol 17 (5) ◽  
pp. 586-590 ◽  
Author(s):  
Sachiko Osuga ◽  
Antoine M. Hakim ◽  
Hitoshi Osuga ◽  
Matthew J. Hogan
Keyword(s):  
Rat Brain ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Control Group ◽  
Similar Measurement ◽  
Contralateral Hemisphere ◽  
Radiotracer Uptake ◽  
Uptake Studies ◽  
In Vivo Uptake

We report autoradiographic measurements of the in vivo uptake of [3H]nimodipine during the nonischemic depolarization of cortical spreading depression (CSD) in rat brain. [3H]Nimodipine uptake in brain was determined regionally in rats undergoing CSD (n = 8) and was significantly increased in cortex (14 ± 7%) and hippocampus (10 ± 6%) on the stimulated side relative to the contralateral hemisphere when compared with the same measurements in a control group (n = 8). A similar measurement using the physiologically inert radiotracer [14C]iodoantipyrine to control for potential effects of CSD on radioligand distribution showed a minimal increase (2.4 ± 0.7%) of radiotracer uptake in cortex after CSD. This increase was significantly less than that observed in the [3H]nimodipine uptake studies. We hypothesize that increased in vivo [3H]nimodipine uptake in CSD identifies regions of depolarization and thus infers activation of the L-type voltage sensitive calcium channels.

scholarly journals In vivo uptake of [14C]leucine by skeletal muscle ribosomes after injury in rats fed two levels of protein

1969 ◽  
Vol 23 (2) ◽  
pp. 271-280 ◽  
Author(s):  
V. R. Young ◽  
P. C. Huang
Keyword(s):  
Skeletal Muscle ◽  
Specific Activity ◽  
Male Rats ◽  
Thigh Muscle ◽  
Left Femur ◽  
The Right ◽  
And Control ◽  
The Relationship ◽  
In Vivo Uptake

1. After 14 days on a diet containing 5 or 25% casein male rats received a fracture of the left femur. Four hours before they were killed the injured and control rats were injected with [1-14C]leucine; the incorporation of radioactivity into an isolated fraction of skeletal muscle ribosomes was studied 6, 12, 24, 48, 72, 96 and 228 h after injury.2. The incorporation of [14C]leucine into the ribosome fraction in right thigh muscles dropped to 40% of control values 72 h after fracture in well-nourished rats and after 96 h with diets containing 5 or 25%, casein.3. The specific activity of the trichloroacetic acid-soluble fraction of muscle from injured rats was equal to or higher than that of the controls during the first 72 h but lower at 96 h.4. These results suggest that a reduced incorporation of amino acids by ribosomes from the right thigh muscle occurred on day 3 after fracture in the group receiving 25% casein but not in the group receiving 5% casein.5. Muscle RNA and DNA concentrations were not affected by the injury.6. The relationship between these findings and the loss of muscle N after injury is discussed.

The in vivo uptake of [3H]isoprenaline by the chick embryonic heart and liver

1980 ◽  
Vol 58 (2) ◽  
pp. 141-146 ◽  
Author(s):  
B. Oštádal ◽  
A. Babický ◽  
J. Kopoldová
Keyword(s):  
Liver Tissue ◽  
Peak Concentration ◽  
Time Course ◽  
Total Radioactivity ◽  
Toxic Metabolites ◽  
Labelled Compound ◽  
Hepatic Lesions ◽  
Second Peak ◽  
In Vivo Uptake

Seven-day-old embryos received 3.7 × 105 Bq (10 μCi) of [3H]isoprenaline (IPRO) hydrochloride intraamnially. The capacity of myocardial and liver tissue for taking up administered catecholamine increased rapidly during the first 10 min after administration. The peak concentration (disintegrations per minute per milligram) in the heart, however, was significantly lower as compared with the liver. Thereafter the uptake in both organs markedly decreased and reached its lowest values between 15 and 30 min. From then on, the concentration of the tritium-labelled compound increased again and 6 h after administration it attained the second peak in both organs. This value was more than five times higher in the liver as compared with the myocardium.Radiometric evaluation of chromatograms from myocardial and liver extracts has revealed that IPRO is rapidly metabolized to 3-O-methyl IPRO. The proportion of this fraction in both organs represents approximately 40% of total radioactivity as early as 5 min after administration.The time course of IPRO uptake indicates that the following factors may participate in the development of cardiac and hepatic lesions: (a) IPRO immediately after administration, and (b) subsequently its toxic metabolites.

Intestinal permeability in allergic rats: nerve involvement in antigen-induced changes

1993 ◽  
Vol 264 (4) ◽  
pp. G617-G623 ◽  
Author(s):  
S. E. Crowe ◽  
K. Soda ◽  
A. M. Stanisz ◽  
M. H. Perdue
Keyword(s):  
Intestinal Permeability ◽  
Fold Increase ◽  
Serum Levels ◽  
Baseline Period ◽  
Antigen Challenge ◽  
51Cr Edta ◽  
Neural Factors ◽  
Induced Changes ◽  
In Vivo Uptake

In vivo uptake of the probe 51Cr-labeled EDTA from the jejunum of egg albumin (EA)-sensitized rats was compared with controls at baseline and after intraluminal antigen challenge. Probe recovery in blood was 60-80% greater in sensitized animals during the baseline period, suggesting that sensitization resulted in increased intestinal permeability. Sensitized, but not control, rats demonstrated a 15-fold increase in 51Cr-EDTA uptake after intraluminal antigen; no change occurred with an unrelated protein. Macromolecular recovery was also enhanced in sensitized animals, since serum levels of immunoreactive EA were elevated 14-fold compared with controls. Antigen challenge was accompanied by biochemical (protease release) and morphological (reduced numbers) evidence of mast cell degranulation in sensitized rats. The neurotoxin tetrodotoxin (applied directly to ligated jejunal segments) inhibited EA-induced uptake of 51Cr-EDTA and antigen. In isolated jejunum from sensitized rats, tetrodotoxin reduced secretory responses to luminal, but not serosal, antigen. These results indicate that neural factors may influence the uptake of molecules from the gut lumen during intestinal anaphylaxis.

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