Inhibition of cell division by blue light

1973 ◽  
Vol 79 (2) ◽  
pp. 318-326 ◽  
Author(s):  
H. Ninnemann ◽  
B. Epel
Keyword(s):  
Cell Division ◽  
Blue Light

scholarly journals Light-powered Escherichia coli cell division for chemical production

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qiang Ding ◽  
Danlei Ma ◽  
Gao-Qiang Liu ◽  
Yang Li ◽  
Liang Guo ◽  
...  
Keyword(s):  
Cell Division ◽  
Blue Light ◽  
Ribonucleotide Reductase ◽  
Near Infrared ◽  
Infrared Light ◽  
Light Activation ◽  
Chemical Production ◽  
E Coli ◽  
Microbial Cell Factories ◽  
Cell Factories

Abstract Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm−1 and acetoin titer to 67.2 g·L−1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L−1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

scholarly journals Coordinating Carbon Metabolism and Cell Cycle of Chlamydomonasreinhardtii with Light Strategies under Nitrogen Recovery

2021 ◽  
Vol 9 (12) ◽  
pp. 2480
Author(s):  
Yuanyuan Ren ◽  
Han Sun ◽  
Jinquan Deng ◽  
Yue Zhang ◽  
Yuelian Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Photosystem Ii ◽  
Blue Light ◽  
Carbon Metabolism ◽  
Cell Cycle Progression ◽  
Red Light ◽  
Nutrient Starvation ◽  
N Recovery ◽  
Cycle Progression

Nutrient supplementation is common in microalgae cultivation to enhance the accumulation of biomass and biofunctional products, while the recovery mechanism from nutrient starvation is less investigated. In this study, the influence of remodeled carbon metabolism on cell cycle progression was explored by using different light wavelengths under N-repletion and N-recovery. The results suggested that blue light enhanced cell enlargement and red light promoted cell division under N-repletion. On the contrary, blue light promoted cell division by stimulating cell cycle progression under N-recovery. This interesting phenomenon was ascribed to different carbon metabolisms under N-repletion and N-recovery. Blue light promoted the recovery of photosystem II and redirected carbon skeletons into proteins under N-recovery, which potentially accelerated cell recovery and cell cycle progression. Although red light also facilitated the recovery of photosystem II, it mitigated the degradation of polysaccharide and then arrested almost all the cells in the G1 phase. By converting light wavelengths at the 12 h of N-recovery with blue light, red and white lights were proved to increase biomass concentration better than continuous blue light. These results revealed different mechanisms of cell metabolism of Chlamydomonas reinhardtii during N-recovery and could be applied to enhance cell vitality of microalgae from nutrient starvation and boost biomass production.

Die lichtabhängige Ausbildung der Zellkerne in den Vorkeimen von Dryopteris filix-mas bei Hemmung der Proteinsynthese

1964 ◽  
Vol 19 (12) ◽  
pp. 1142-1146 ◽  
Author(s):  
Rainer Bergfeld
Keyword(s):  
Cell Division ◽  
Cell Differentiation ◽  
Mitotic Activity ◽  
Blue Light ◽  
Normal Cell ◽  
Cell Metabolism ◽  
Red Light ◽  
Two Dimensional

It has been shown in a number of papers that the young gametophytes of ferns, e. g. Dryopteris filix-mas, will grow differently under red and under blue light. In red light these sporelings grow as filaments whereas under blue light they form two-dimensional normal prothallia. - The development of the sporelings can be inhibited by the application of methyl-tryptophane. The process of cell division is blocked by this treatment. The blue grown sporelings are much more sensitive than the red grown one.Under the influence of the antimetabolite methyl-tryptophane the nuclei will swell in both types of sporelings to about the same extent. The application of tryptophane reverses the effects of the antimetabolite on nuclear swelling, cell division and development in general. In the case of the blue grown sporelings much higher concentrations of tryptophane were needed to reverse the inhibition mediated by methyl-tryptophane.It is assumed that the application of methyl-tryptophane leads to a disintegration of protein and cell metabolism. The transport of substances from the nucleus to the cytoplasm is blocked under these conditions. The result is an accumulation of material in the nucleus which leads to swelling and finally to an inactivation of the nucleus as far as cell division and cell differentiation are concerned. The application of tryptophane gradually leads to a reconstitution of the normal cell metabolism. The swelling of the nucleus is reversed. Apparently the flow of material from the nucleus to the cytoplasm is taken up again which results in normal cell differentiation and mitotic activity.

scholarly journals Long-term Blue Light Effects on the Histology of Lettuce and Soybean Leaves and Stems

2004 ◽  
Vol 129 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Tracy A.O. Dougher ◽  
Bruce Bugbee
Keyword(s):  
Cell Division ◽  
Leaf Area ◽  
Blue Light ◽  
Cell Expansion ◽  
Light Exposure ◽  
Stem Elongation ◽  
Fold Increase ◽  
Light Fraction ◽  
Short Term

Blue light (320 to 496 nm) alters hypocotyl and stem elongation and leaf expansion in short-term, cell-level experiments, but histological effects of blue light in long-term studies of whole plants have not been described. We measured cell size and number in stems of soybean (Glycine max L.) and leaves of soybean and lettuce (Lactuca sativa L.), at two blue light fractions. Short-term studies have shown that cell expansion in stems is rapidly inhibited when etiolated tissue is exposed to blue light. However, under long-term light exposure, an increase in the blue light fraction from <0.1% to 26% decreased internode length, specifically by inhibiting soybean cell division in stems. In contrast, an increase in blue light fraction from 6% to 26% reduced soybean leaf area by decreasing cell expansion. Surprisingly, lettuce leaf area increased with increasing blue light fraction (0% to 6%), which was attributed to a 3.1-fold increase in cell expansion and a 1.6-fold increase in cell division.

Blue Light Delays Commitment to Cell Division in Chlamydomonas reinhardtii

Plant Biology ◽  
2004 ◽  
Vol 6 (6) ◽  
pp. 689-695 ◽  
Author(s):  
H. Oldenhof ◽  
V. Zachleder ◽  
H. Ende
Keyword(s):  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Commitment To Cell Division
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