MTT colorimetric assay for testing macrophage cytotoxic activity in vitro

AbstractInfectious bursal disease (IBD) caused by non-enveloped double stranded RNA virus is an acute and contagious poultry disease. Outbreak of IBD could result in 10–75% mortality of the birds; hence it has gained socio-economic importance worldwide. Medicinal plants have shown broad spectrum anti-viral activities against RNA and DNA viruses. Moringa oleifera Lam (MOL), Phyllanthus emblicus Linn (PEL), Glycyrrhiza glabra Linn (GGL), and Eugenia jambolana Lam (EJL) are commonly available medicinal plants of the sub-continent and exhibited anti-viral potential against different viruses. Ethanolic extracts of the leaves of MOL and EJL, roots of GGL and dried fruit of PEL were investigated for their cytotoxic and anti-viral potential against IBD virus using MTT colorimetric assay and anti-viral assay. Significant anti-viral potential (P<0.001) was demonstrated at concentrations 12.5, 25, 50 and 100 µg ml−1 of GGL, PEL, MOL and EJL, respectively, with no cytotoxicity. Data also spotlighted that all tested plant extracts possess significant anti-viral potential and this trend was higher in GGL followed by PEL, MOL, and EJL. The data undoubtedly conclude that these medicinal plants contain several health beneficial phyto-chemicals which got significant anti-viral potential and effectively be utilized against IBD virus. Moreover, the outcomes of this study provide a platform on the way to discover novel anti-viral agents against IBD virus and other viruses from plant origin.

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Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

Brazilian Dental Journal ◽

10.1590/0103-6440201902542 ◽

2019 ◽

Vol 30 (3) ◽

pp. 201-207 ◽

Cited By ~ 2

Author(s):

Francine Lorencetti-Silva ◽

Priscilla Aparecida Tartari Pereira ◽

Alyne Fávero Galvão Meirelles ◽

Lúcia Helena Faccioli ◽

Francisco Wanderley Garcia Paula-Silva

Keyword(s):

Stem Cells ◽

Prostaglandin E2 ◽

Dental Pulp ◽

Colorimetric Assay ◽

Dental Pulp Stem Cells ◽

Lipid Mediator ◽

Water Emulsion ◽

Mtt Colorimetric Assay ◽

Oil In Water Emulsion

Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells.

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Cytotoxic Activity of Lomatiol and 7-(3′-Hydroxymethyl-3′-methylallyloxy)coumarin

Natural Product Communications ◽

10.1177/1934578x1601100322 ◽

2016 ◽

Vol 11 (3) ◽

pp. 1934578X1601100

Author(s):

Serena Fiorito ◽

Salvatore Genovese ◽

Francesco Epifano ◽

Veronique Mathieu ◽

Robert Kiss ◽

...

Keyword(s):

Structural Feature ◽

Colorimetric Assay ◽

Parent Compound ◽

Side Chain ◽

In Vitro Growth ◽

Mtt Colorimetric Assay ◽

Growth Inhibitory ◽

Inhibitory Activities ◽

Comparison Data

7-(3′-Hydroxymethyl-3′-methylallyloxy)coumarin, bearing a 3′-hydroxy-3′-methylallyl group as the O-side chain, and lomatiol, a lapachol derivative sharing the same structural feature, were tested for their in vitro growth inhibitory activities on six cancer cell lines using the MTT colorimetric assay, along with the respective 3′,3′-dimethylallyl derivatives and unprenylated products used for comparison. Data revealed that lomatiol displayed the strongest growth inhibitory activities in vitro although not as efficient as the parent compound lapachol. The oxidized O-prenylcoumarin recorded better growth inhibitory capacities than the prenylated and unprenylated parent products.

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In Vitro Assessment of Antibacterial Activity and Cytotoxicity of Silver Contained Antibacterial HA Coating Material

Materials Science Forum ◽

10.4028/www.scientific.net/msf.620-622.307 ◽

2009 ◽

Vol 620-622 ◽

pp. 307-310 ◽

Cited By ~ 6

Author(s):

Zi Yuan Zhu ◽

Fu Qiang Zhang ◽

You Tao Xie ◽

Yi Kai Chen ◽

Xue Bin Zheng

Keyword(s):

Antibacterial Activity ◽

Zirconium Phosphate ◽

Pathogenic Bacteria ◽

Colorimetric Assay ◽

Vacuum Plasma Spraying ◽

Vacuum Plasma ◽

Mtt Colorimetric Assay ◽

Ha Coating ◽

Covering Method

Failure of dental implant is often caused by specific pathogenic bacteria. In this paper, antibacterial HA coating was prepared by vacuum plasma spraying with silver loaded zirconium phosphate containing HA as feedstock. The antibacterial activity against Aa，Fn and Pg was evaluated using a film covering method. MTT colorimetric assay was used to measure cytotoxic effect of the antibacterial HA coatings. Results show that the HA coatings displayed significant antibacterial activity against Aa，Fn, Pg and no cytotoxicity to L929 murine cell lines when the content of silver-loaded zirconium phosphate antimicrobial is between 5wt% to 10wt%. The sensitivity of the bacteria to the antibacterial HA coating is in sequence of Pg >Fn >Aa.

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In vitro trypanocidal activity of solamargine and extracts from Solanum palinacanthum and Solanum lycocarpum of brazilian cerrado

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652013000300006 ◽

2013 ◽

Vol 85 (3) ◽

pp. 903-907 ◽

Cited By ~ 5

Author(s):

RAQUEL R.D. MOREIRA ◽

GILMARCIO Z. MARTINS ◽

NATHALIA O. MAGALHAES ◽

ADELIA E. ALMEIDA ◽

ROSEMEIRE C.L.R. PIETRO ◽

...

Keyword(s):

Chagas Disease ◽

Colorimetric Assay ◽

Ethanolic Extract ◽

Brazilian Cerrado ◽

Strong Activity ◽

Trypanocidal Activity ◽

Mtt Colorimetric Assay ◽

Ethanol Extracts ◽

First Time

The present investigation was to evaluate the potential trypanocidal activity of crude ethanolic extract of the fruits of Solanum palinacanthum, Solanum lycocarpum and the glycoalcaloid, solamargine. S. palinacanthum and S. lycocarpum fruit powders were submitted to exhaustively extraction with 96% ethanol and solamargine were isolated from the extract of S. palinacanthum. Both extracts and solamargine were analysed for trypanocidal activity by using MTT colorimetric assay. Extracts of S. palinacanthum showed to be more active (IC50 = 175.9 µg.ml–1) than S. lycocarpum (IC50 = 194.7 µg.ml–1). Solamargine presented a strong activity (IC50 = 15.3 µg.ml–1), which can explain the better activity of the both extracts. Benznidazol (IC50 = 9.0 µg.ml–1) is the only drug used to treat Chagas' disease. These findings demonstrate for the first time that ethanol extracts obtained from both fruits of S. palinacanthum and S. lycocarpum and also solamargine have a potential anti-trypanosomal activity.

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A New Xanthone from the Pericarp of Garcinia mangostana

Natural Product Communications ◽

10.1177/1934578x1300801219 ◽

2013 ◽

Vol 8 (12) ◽

pp. 1934578X1300801 ◽

Cited By ~ 2

Author(s):

Manqin Fu ◽

Samuel X. Qiu ◽

Yujuan Xu ◽

Jijun Wu ◽

Yulong Chen ◽

...

Keyword(s):

Colorimetric Assay ◽

Human Cancer ◽

Ethanol Extract ◽

2D Nmr ◽

Hep G2 ◽

Garcinia Mangostana ◽

Human Cancer Cell Lines ◽

Mtt Colorimetric Assay ◽

Mcf 7

A new prenylxanthone, garcimangostanol (1), was isolated from the EtOAc-soluble partition of the ethanol extract of the pericarp of Garcinia mangostana L., along with three known compounds, namely 8-deoxygartanin (2), 1-isomangostin (3), and garcinone C (4). The structure of compound 1 was elucidated on the basis of its 1D, 2D NMR and MS data. Compounds 1–4 exhibited either significant or moderate cytotoxicity against MCF-7, A549, Hep-G2 and CNE human cancer cell lines in vitro with IC50 values from 4.0 ± 0.3 to 23.6 ± 1.5 μM by MTT colorimetric assay.

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In Vitro Anti-proliferative Effect of Naturally Occurring Oxyprenylated Chalcones

Natural Product Communications ◽

10.1177/1934578x1300800814 ◽

2013 ◽

Vol 8 (8) ◽

pp. 1934578X1300800 ◽

Cited By ~ 3

Author(s):

Serena Fiorito ◽

Francesco Epifano ◽

Celine Bruyère ◽

Robert Kiss ◽

Salvatore Genovese

Keyword(s):

Natural Products ◽

Secondary Metabolites ◽

Cell Lines ◽

Mechanism Of Action ◽

Colorimetric Assay ◽

Appreciable Effect ◽

Proliferative Effect ◽

Mtt Colorimetric Assay ◽

Naturally Occurring

As a continuation of our ongoing studies aimed to depict the effects and mechanism of action of naturally occurring oxyprenylated phenylpropanoids and polyketides, in this paper we describe the synthesis and in vitro anti-proliferative effects of selected compounds belonging to the above cited classes of secondary metabolites on six cancer cell lines using the MTT colorimetric assay. Our study revealed that among the natural products tested, only oxyprenylated chalcones exhibited an appreciable effect (mean IC50 = 32 - 64 μM), while substituted alcohols, phenylpropenes, naphthoquinones, and aminoacid derivatives were by far less active or inactive.

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In Vitro Evaluation of the Cytotoxicity of Some New Titanium Alloys

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.587.303 ◽

2013 ◽

Vol 587 ◽

pp. 303-308 ◽

Cited By ~ 2

Author(s):

Andreea Carmen Bărbînţă ◽

Kamel Earar ◽

Carmen Iulia Crimu ◽

Lucia Anişoara Drăgan ◽

Corneliu Munteanu

Keyword(s):

Titanium Alloys ◽

Biomedical Applications ◽

Colorimetric Assay ◽

Osteosarcoma Cell ◽

Human Osteosarcoma Cell Line ◽

Cytotoxic Effects ◽

Human Osteosarcoma ◽

Mtt Colorimetric Assay ◽

Human Osteosarcoma Cell

Titanium alloys are widely used in medical applications, due to their good mechanical properties, high corrosion resistance and biocompatibility. The aim of this paper was to investigate the cytotoxicity of novel titanium alloys: Ti21Nb6Zr15Ta, Ti25Nb10Zr8Ta, Ti17Nb5Zr5Al, Ti7Nb7Zr2Al with fibroblast-like cells derived from human osteosarcoma cell line (HOS). The results were compared with that of conventional biomedical alloys, like Ti6Al7Nb and Ti6Al4V. In vitro citotoxicity of titanium alloys was evaluated by fluorescence microscopy and MTT colorimetric assay. The results showed that the materials analyzed had no cytotoxic effects on HOS fibroblast-like cells, permitting their attachment and proliferation. Also the new titanium alloys present a higher cell viability than that of the conventional alloys. As a consequence, the TiNbZrTa and TiNbZrAl alloys can be considered as potential candidates for biomedical applications.

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SYNTHESIS OF SOME 2,3-DIHYDRO-1,3,4-OXADIAZOLES AND 4,5-DIHYDRO-1,2,4-TRIAZOLES AS ANTICANCER AGENTS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i8.36508 ◽

2020 ◽

pp. 92-99

Author(s):

TAWFEEK A. YAHYA ◽

JALAL H. ABDULLAH

Keyword(s):

Cytotoxic Activity ◽

Anticancer Agents ◽

Colorimetric Assay ◽

Human Cancer ◽

Cancer Cell Line ◽

Cytotoxic Activities ◽

Standard Drug ◽

Mass Spectral ◽

Oxadiazole Derivatives

Objective: The main objective of this work was to synthesize and evaluate the novel 2,3-dihydro-1,3,4-oxadiazole and 4,5-dihydro-1,2,4-triazole derivatives for cytotoxic activities. Methods: The 2,3-dihydro-1,3,4-oxadiazole derivatives 4a-h were synthesized by cyclization of N'-(substituted-benzylidene) isonicotinohydrazide 3a-e in refluxing acetic anhydride. The 2,3-dihydro-1,3,4-oxadiazole derivatives 4a-h were converted into the corresponding 4,5-dihydro-1,2,4-triazoles 5a-h using ammonia. All the synthesized compounds were identified, depending on the physical and spectral data. Title compounds were assessed for their cytotoxic activity against human cancer cell line (MCF-7) by using Sulforhodamine B (SRB) colorimetric assay. Results: All the synthesized compounds showed characteristic peaks in FTIR, 1HNMR and Mass spectral analysis. The results of the in vitro cytotoxic activity revealed that the compound 4c exhibited equipotent cytotoxic activity with an IC50 value of 8.04 µM when compared with that of standard drug doxorubicin (IC50= 8.02 µM). The reminder compounds have shown good to moderate cytotoxic activities when compared with that of a reference standard. Conclusion: We synthesized a series of title compounds in quantitative yields. Most derivatives showed moderate to good cytotoxic activity.

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Antiviral Activity of Total Flavonoid Extracts fromSelaginella moellendorffiiHieron against Coxsackie Virus B3In VitroandIn Vivo

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/950817 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Dan Yin ◽

Juan Li ◽

Xiang Lei ◽

Yimei Liu ◽

Zhanqiu Yang ◽

...

Keyword(s):

Antiviral Activity ◽

Inhibitory Concentration ◽

Colorimetric Assay ◽

Total Flavonoid ◽

Coxsackie Virus ◽

Mtt Colorimetric Assay ◽

Diphenyl Tetrazolium Bromide ◽

Selaginella Moellendorffii

The antiviral activity of total flavonoid extracts fromSelaginella moellendorffiiHieron and its main constituents amentoflavone were investigated against coxsackie virus B3 (CVB3). When added during or after viral infection, the extracts and amentoflavone prevented the cytopathic effect (CPE) of CVB3, as demonstrated in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay, with a 50% inhibitory concentration (IC50) from19±1.6to41±1.2 μg/mL and25±1.2to52±0.8 μg/mL, respectively. KM mice were used as animal models to test the extracts' activityin vivo. Oral administration of the total flavonoid extracts at 300 mg/kg/day significantly reduced mean viral titers in the heart and kidneys as well as mortality after infection for 15 days. The experimental results demonstrate thatin vitroandin vivothe model mice infected with CVB3 can be effectively treated by the total flavonoid extracts fromSelaginella moellendorffiiHieron.

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