Tail-cuff method for measuring systolic blood pressure in squirrel monkeys

1. The non-invasive measurement of tail cuff systolic blood pressure in conscious rats is routinely used in long-term cardiovascular studies. There are a number of commercially available tail cuff systems, however, these apparatus are generally expensive and are dedicated for single-task operations. In the present study, a simple method for measuring systolic blood pressure, which requires only minor modifications to the existing hardware found in most cardiovascular laboratories, is described. 2. Systolic blood pressure measurements were made in the conventional manner by determining the systolic blood pressure which coincided with the restoration of the caudal artery pulse. This was achieved by using an inexpensive piezo-electric pulse transducer to detect the pulse, and this was coupled to a standard data-aquisition system (MacLab®, ADInstruments) normally set up to record blood pressure. This method was compared with another established tail cuff method, as well as with direct intra-arterial recordings. 3. It was found that the results obtained using both tail cuff systems were in good agreement when systolic blood pressure was measured in Wistar—Kyoto rats and spontaneously hypertensive rats. In addition, systolic blood pressure was measured over 4 weeks in 2K1C rats and sham-operated rats, with both tail cuff methods producing similar results, which were not significantly different from direct intra-arterial recordings in the same animals. 4. Thus, in the present study, with only minor modifications, the same equipment was used for both direct and indirect determinations of systolic blood pressure. This situation differs from other conventional tail cuff systems since these items are designed for a single purpose. Therefore, the current method using piezo-electric sensor/MacLab®-technology should be viewed as a relatively simple, flexible and cheap alternative method to measure tail cuff systolic blood pressure in conscious rats.

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Hypertension and Impaired Glycine Handling in Mice Lacking the Orphan Transporter XT2

Molecular and Cellular Biology ◽

10.1128/mcb.24.10.4166-4173.2004 ◽

2004 ◽

Vol 24 (10) ◽

pp. 4166-4173 ◽

Cited By ~ 41

Author(s):

Hui Quan ◽

Krairerk Athirakul ◽

William C. Wetsel ◽

Gonzalo E. Torres ◽

Robert Stevens ◽

...

Keyword(s):

Mass Spectrometry ◽

Blood Pressure ◽

High Pressure ◽

Liquid Chromatography ◽

Systolic Blood Pressure ◽

Knockout Mice ◽

Wild Type ◽

Low Salt ◽

Organic Solute ◽

Tail Cuff

ABSTRACT A family of orphan transporters has been discovered that are structurally related to the Na+-Cl−-dependent neurotransmitter transporters, including the dopamine transporter. One member of this family, the mouse XT2 gene, is predominantly expressed in the kidney and has 95% homology to rat ROSIT (renal osmotic stress-induced Na+-Cl− organic solute cotransporter). To study the physiological functions of this transporter, we generated XT2-knockout mice by gene targeting. XT2−/− mice develop and survive normally with no apparent abnormalities. To attempt to identify potential substrates for XT2, we screened urine from XT2-knockout mice by high-pressure liquid chromatography and mass spectrometry and found significantly elevated concentrations of glycine. To study glycine handling, XT2+/+ and XT2−/− mice were injected with radiolabeled glycine, and urine samples were collected to monitor glycine excretion. After 2 h, XT2−/− mice were found to excrete almost twice as much glycine as the XT2+/+ controls (P = 0.03). To determine whether the absence of the XT2 transporter affected sodium and fluid homeostasis, we measured systolic blood pressure by computerized tail-cuff manometry. Systolic blood pressure was significantly higher in XT2−/− mice (127 ± 3 mmHg) than in wild-type controls (114 ± 2 mmHg; P < 0.001). This difference in systolic blood pressure was maintained on high and low salt feeding. To examine whether the alteration in blood pressure and the defect in glycine handling were related, we measured systolic blood pressure in the XT2−/− mice during dietary glycine supplementation. Glycine loading caused systolic blood pressure to fall in the XT2−/− mice from 127 ± 3 to 115 ± 3 mmHg (P < 0.001), a level virtually identical to that of the wild-type controls. These data suggest that the XT2 orphan transporter is involved in glycine reabsorption and that the absence of this transporter is sufficient to cause hypertension.

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Serial analysis of gene expression in mouse kidney following angiotensin II administration

Physiological Genomics ◽

10.1152/physiolgenomics.00108.2003 ◽

2003 ◽

Vol 16 (1) ◽

pp. 90-98 ◽

Cited By ~ 7

Author(s):

Faina Schwartz ◽

Arvi Duka ◽

Elena Triantafyllidi ◽

Conrado Johns ◽

Irena Duka ◽

...

Keyword(s):

Gene Expression ◽

Blood Pressure ◽

Angiotensin Ii ◽

Systolic Blood Pressure ◽

Molecular Mechanisms ◽

Mouse Kidney ◽

Ang Ii ◽

Disease States ◽

Genome Wide ◽

Tail Cuff

As a new line of inquiry into the molecular mechanisms underlying pathophysiological processes associated with angiotensin (ANG II)-dependent hypertension, we applied the method of serial analysis of gene expression (SAGE) to examine genome-wide transcription changes in the kidneys of mice that developed hypertension in response to chronic ANG II administration. Mice were infused subcutaneously via osmotic minipumps with ANG II for 7 days, and systolic blood pressure was measured by tail-cuff plethysmography. Subsequently, mice were euthanized, and the total RNA isolated from the kidneys was used to construct SAGE libraries. Comparison of 11,447 SAGE tags from the hypertensive kidneys, representing 5,740 unique transcripts, and 11,273 tags from the control kidneys, corresponding to 5,619 different transcripts, identified genes that are significantly ( P < 0.05) down- or upregulated in the hypertensive kidney. Our assessment of the genome-wide influence of ANG II resulted in the detection of several novel genes and in a recognition of potential new roles for the previously characterized genes, thus providing new probes with which to further explore the ANG II effects in normal and disease states.

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Is a systolic blood pressure target <140mmHg indicated in all hypertensives? Subgroup analyses of findings from the randomized FEVER trial

Yearbook of Cardiology ◽

10.1016/j.ycar.2012.01.084 ◽

2012 ◽

Vol 2012 ◽

pp. 96-98

Author(s):

W.J. Elliott

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Blood Pressure Target ◽

Subgroup Analyses

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P3301 Rise in systolic blood pressure during exercise test and the risk of acute coronary events in middle-aged men?

European Heart Journal ◽

10.1016/s0195-668x(03)95927-6 ◽

2003 ◽

Vol 24 (5) ◽

pp. 628

Author(s):

J LAUKKANEN

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Exercise Test ◽

Middle Aged ◽

Coronary Events

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Paradoxical Hypotension during Dobutamine Infusion for Myocardial Perfusion Scintigraphy

Nuklearmedizin ◽

10.1055/s-0038-1632347 ◽

1998 ◽

Vol 37 (08) ◽

pp. 268-271

Author(s):

B. Caner ◽

E. Atalar ◽

A. Karanfil ◽

L. Tokgözoğlu ◽

E. L. Ergün

Keyword(s):

Blood Pressure ◽

Myocardial Perfusion ◽

Systolic Blood Pressure ◽

Myocardial Perfusion Scintigraphy ◽

Stress Test ◽

Dobutamine Infusion ◽

Perfusion Scintigraphy ◽

Tilt Table ◽

Study Results ◽

Group A

Summary Aim: Dobutamine as a predominant beta-1 agonist increases heart rate and myocardial contractility and at sufficient high doses, it also increases systolic blood pressure. This study was undertaken to describe instances of paradoxical hypotension during dobutamine infusion for TI-201 myocardial perfusion SPECT study and the relationship between scintigraphic findings and hypotension occurred during dobutamine infusion. Methods: In 201 consecutive patients unable to perform adequate exercise, dobutamine TI-201 myocardial SPECT was performed. Dobutamine was infused starting from 10 μg/kg/min increasing to 40 μg/kg/min. Paradoxical hypotension was defined as a decrease in systolic blood pressure ≥ 20 mmHg compared with baseline study. Results: Paradoxical hypotension was observed in 40 patients (Group A) out of 201 (19.9%) while no significant change in systolic blood pressure was detected in the remaining 161 patients (Group B). Mean maximum fall in systolic blood pressure was 39 ± 18 mmHg (range: 20-90). In 33 of 40 patients (83%) with paradoxical hypotension, scintigraphy was normal compared to 131 (81%) of the remaining 161 patients. In patients of Group A, angiography, echocardiography and tilt table tests were performed in 13, 11 and 6 patients respectively. Nine of 13 angiographic evaluations (69%), 10 of 11 echocardiographic evaluations (91%), all of the tilt table tests were normal. Additionally, all of the patients of Group A were clinically followed up at least 6 months after the myocardial perfusion scintigraphy. None of the patients had a cardiac event except one patient during the follow-up period. Conclusion: Paradoxical hypotension during dobutamine infusion for myocardial scintigraphy is not an uncommon finding and up to 19.9% patients may develop such hypotension. To maximize test safety, precautions should be taken during dobutamine myocardial stress test, since remarkable decrease in systolic blood pressure may occur. Unlike hypotension occurring with exercise test, hypotension response to dobutamine is not always a marker for coronary artery disease.

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INTERARM SYSTOLIC BLOOD PRESSURE DIFFERENCE IS ASSOCIATED WITH A HIGH PREVALENCE OF CARDIO VASCULAR DISEASES- A CROSS-SECTIONAL STUDY IN POLICE WORKER

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i11.1524 ◽

2020 ◽

Vol 4 (11) ◽

Author(s):

Somesh Raju ◽

Rina Kumari ◽

Sunita Tiwari ◽

NS Verma

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Blood Sugar ◽

Pressure Difference ◽

Systolic Pressure ◽

Working Environment ◽

Vascular Events ◽

Cross Sectional ◽

Blood Pressure Difference ◽

High Prevalence

Background: Interarm systolic blood pressure difference more than 10 mm of Hg is predictor of cardiovascular and metabolic risk. Despite of sufficient physical activity there is high prevalence of obesity in police personal because of stressful working environment. No studies have addressed the significance of interarm pressure difference among them. Therefore, the present study conducted to access the relation of interarm blood pressure difference with obesity in police personnel. Aims and Objective: To estimate the interarm pressure difference in police personal to see its association with their obesity. Material and Method: This cross-sectional observational study done on 245 police workers in PAC, Sitapur, India. Subjects having more than ten years of working experience were included in study. Anthropometric measurements of subject recorded by following standard protocol. Measurement of systolic pressure in both arm recorded simultaneously by mercury sphygmomanometer. Available data analyze and expressed in percentage, mean with SD and chi square test to see the significance of association. Results: According to anthropometric results of subjects 77.14 prcent subjects were having generalised obesity and 82.04 percent of subjects having abdominal obesity. 34.29 percent of subjects showed abnormally high (?10 mmHg) inter-arm systolic blood pressure difference. Both type of obesity showed positive association with blood sugar level but no association with interarm pressure difference. Conclusion: Interarm blood pressure difference is greater in individual having obesity or prolong duration of service. Such subjects are more susceptible to develop coronary artery disease or peripheral arterial disease in future. Early screening can help to detect the vascular events likely to occur in the future Keywords: Body mass index, Waist circumference, Interarm pressure difference, Random blood sugar

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EFEKTIVITAS PEMBERIAN HYDROTHERAPI RENDAM KAKI PADA PENDERITA HIPERTENSI DI BANJAR SRI MANDALA, KELURAHAN DAUHWARU KABUPATEN JEMBRANA

JURNAL WIDYA BIOLOGI ◽

10.32795/widyabiologi.v10i01.235 ◽

2019 ◽

Vol 10 (01) ◽

pp. 33-44

Author(s):

N.L.G. Sudaryati ◽

I P. Sudiartawan ◽

Dwi Mertha Adnyana

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Diastolic Blood Pressure ◽

Pressure Measurement ◽

Blood Pressure Measurement ◽

Reduce Blood Pressure ◽

Second Grade ◽

Control Group ◽

Hypertensive Patients ◽

First Category

The aim of the study was to determine the effectiveness of giving hydrotherapi foot soak in hypertensive patients. The study was conducted with one group pretestposttest design without a control group by measuring blood pressure (pretest) before being given an intervention in the form of foot soak hydrotherm against 15 people with hypertension in Banjar Sri Mandala, Dauhwaru Village, Jembrana Subregency. Then do the blood pressure measurement again (posttest) after finishing the intervention. After the study was completed, the results showed that before the hydrotherapi foot bath intervention, there were 0% of patients classified as normal, 13.32% in prehypertension category, 60.08% in hypertension category I and 26.60% in hypertension category II. After the intervention was given, there were 13.32% of the patients classified as normal, 66.68% in the prehypertension category, 20.00% in the first category of hypertension and no patients belonging to the second grade hypertension category. There is a decrease of 20-30 mmHg for systolic blood pressure and 0-10 mmHg for diastolic blood pressure after intervention. Based on the results of the study it can be concluded that the hydrotherapi foot bath is effectively used to reduce blood pressure in hypertensive patients in the Banjar Sri Mandala, Dauhwaru Village, Jembrana District.

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