Simultaneous targeted and untargeted UHPLC-ESI-MS/MS method with data-independent acquisition for quantification and profiling of (oxidized) fatty acids released upon platelet activation by thrombin

(1) Background: this study describes bioactive compounds in the following halophytes: Sarcocornia (S. alpini, S. pruinosa, and S. perennis) and Arthrocnemum (A. macrostachyum). The material comes from: coastal marshes in Tinto River, Guadiana River, and some interior provinces from the Iberian Peninsula. (2) Methods: the techniques used were Folin–Ciocalteu, GC-MS, and ESI-MS/MS. (3) Results: Five phenolic acids were found in Sarcocornia: trans-cinnamic, salicylic, veratric, coumaric, and caffeic acids. In addition, in Arthronemum, ferulic acid was also detected. The obtained flavonoids were cyanidin-3-O-arabinoside, luteolin-7-glucoside, dihydroquercetin, and p-coumaroyl-glucoside. They also presented fatty acids, such as palmitic, linoleic, and oleic acids in Sarcocornia, while palmitic, linolenic, and stearic acids were the main fatty acids in A. macrostachyum. (4) Conclusions: the high diversity of the compounds identified confirms the relation between nutritional interest and salt tolerance in halophytes.

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Chemical Profiles of Cultivated Agarwood Induced by Different Techniques

Molecules ◽

10.3390/molecules24101990 ◽

2019 ◽

Vol 24 (10) ◽

pp. 1990 ◽

Cited By ~ 1

Author(s):

Tingting Yan ◽

Sheng Yang ◽

Yuan Chen ◽

Qian Wang ◽

Gaiyun Li

Keyword(s):

Fatty Acids ◽

Aromatic Compounds ◽

Chemical Constituents ◽

Aquilaria Sinensis ◽

Chemical Inducers ◽

Esi Ms ◽

Study Results ◽

Chemical Profiles

Agarwood is the resinous wood produced in some Aquilaria species and is highly valued for wide usages in medicine, incense, and perfume. To protect the threatened Aquilaria species, the cultivation of Aquilaria sinensis and artificial agarwood induction techniques have been effectively established in China. To evaluate the quality of agarwood induced by different techniques, patterns of chemical constituents in artificial agarwood by four methods (wounding using an axe, burning-chisel-drilling, chemical inducer, and biological inoculation) were analyzed and compared by UPLC-ESI-MS/MS and GC-EI-MS in this study. Results of GC-MS gave a panorama of chemical constituents in agarwood, including aromatic compounds, steroids, fatty acids, sesquiterpenoids, and 2-(2-phenlyethyl)-chromones (PECs). Sesquiterpenoids were dominant in agarwood induced by wounding using an axe. PEC comprised over 60% of components in agarwood produced by biological inoculation and chemical inducers. PECs were identified by UPLC-ESI-MS/MS in all artificial agarwood and the relative contents varied in different groups. Tetrahydro-2-(2-phenylethyl)-chromones (THPECs) in wounding by axes induced agarwood were lower while 2-(2-phenylethyl)-chromones (FPECs) were higher than other groups. The results showed that methods used for inducing agarwood formation in Aquilaria sinensis affect the chemical constituents of agarwood.

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Evaluation of possible fraud in avocado oil-based products from the composition of fatty acids by GC-FID and lipid profile by ESI-MS

Chemical Papers ◽

10.1007/s11696-020-01119-z ◽

2020 ◽

Vol 74 (9) ◽

pp. 2799-2812

Author(s):

Adriela A. Rydlewski ◽

Jessica S. Pizzo ◽

Luciana P. Manin ◽

Marília B. Galuch ◽

Patrícia D. S. Santos ◽

...

Keyword(s):

Fatty Acids ◽

Lipid Profile ◽

Esi Ms ◽

Avocado Oil

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Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production

International Journal of Molecular Sciences ◽

10.3390/ijms21228772 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8772

Author(s):

Eugene A. Osae ◽

Tiffany Bullock ◽

Madhavi Chintapalati ◽

Susanne Brodesser ◽

Samuel Hanlon ◽

...

Keyword(s):

Fatty Acids ◽

High Fat Diet ◽

Saturated Fatty Acids ◽

Principal Component ◽

Meibomian Gland ◽

Epididymal Adipose Tissue ◽

Esi Ms ◽

Diet Induced Obesity ◽

Tear Production ◽

Lipid Species

Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity where mice develop dyslipidemia. Methods: Male C57/BL6 mice (8/group, age = 6 wks) were fed a normal (ND; 15% kcal fat) or an obesogenic high-fat diet (HFD; 42% kcal fat) for 10 wks. Tear production was measured and meibography was performed. Body and epididymal adipose tissue (eAT) weights were determined. Nano-ESI-MS/MS and LC-ESI-MS/MS were used to detect CE, TG, Cer and SM species. Data were analyzed by principal component analysis, Pearson’s correlation and unpaired t-tests adjusted for multiple comparisons; significance set at p ≤ 0.05. Results: Compared to ND mice, HFD mice gained more weight and showed heavier eAT and dyslipidemia with higher levels of plasma CE, TG, Cer and SM. HFD mice had hypertrophic meibomian glands, increased levels of lipid species acylated by saturated fatty acids in plasma and meibum and excessive tear production. Conclusions: The majority of meibum lipid species with saturated fatty acids increased with HFD feeding with evidence of meibomian gland hypertrophy and excessive tearing. The dyslipidemia is associated with altered meibum composition, a key feature of MGD.

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Studies on the nature of the taste of lipids. Part VII. for Part VI, see ref. 1. Bitter taste of oxidized fatty acids.

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.44.2477 ◽

1980 ◽

Vol 44 (10) ◽

pp. 2477-2481 ◽

Cited By ~ 2

Author(s):

Riichiro USUKI ◽

Takashi KANEDA

Keyword(s):

Fatty Acids ◽

Bitter Taste ◽

Oxidized Fatty Acids

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The insectivorous bat Pipistrellus nathusii uses a mixed-fuel strategy to power autumn migration

Proceedings of The Royal Society B Biological Sciences ◽

10.1098/rspb.2012.0902 ◽

2012 ◽

Vol 279 (1743) ◽

pp. 3772-3778 ◽

Cited By ~ 32

Author(s):

Christian C. Voigt ◽

Karin Sörgel ◽

Jurģis Šuba ◽

Oskars Keišs ◽

Gunārs Pētersons

Keyword(s):

Fatty Acids ◽

Stable Carbon Isotope ◽

Carbon Isotope Ratio ◽

Dietary Fatty Acids ◽

Autumn Migration ◽

Dietary Proteins ◽

Mixed Fuel ◽

Feeding Experiments ◽

Body Reserves ◽

Oxidized Fatty Acids

In contrast to birds, bats are possibly limited in their capacity to use body fat as an energy source for long migrations. Here, we studied the fuel choice of migratory Pipistrellus nathusii (approximate weight: 8 g) by analysing the stable carbon isotope ratio ( δ 13 C V-PDB ) of breath and potential energy sources. Breath δ 13 C V-PDB was intermediate between δ 13 C V-PDB of insect prey and adipocyte triacylglycerols, suggesting a mixed-fuel use of P. nathusii during autumn migration. To clarify the origin of oxidized fatty acids, we performed feeding experiments with captive P. nathusii . After an insect diet, bat breath was enriched in 13 C relative to the bulk and fat portion of insects, but not deviating from the non-fat portion of insects, suggesting that bats oxidized exogenous proteins and carbohydrates, but not exogenous fatty acids. A feeding experiment with 13 C-labelled substrates confirmed these findings. In conclusion, migratory P. nathusii oxidized dietary proteins directly from insects captured en route in combination with endogenous fatty acids from adipocytes, and replenished their body reserves by routing dietary fatty acids to their body reserves.

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