Self-assembled supramolecular polymers with tailorable properties that enhance cell attachment and proliferation

AbstractHuman keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.

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Poly(L-Lactic Acid-Co-Aspartic Acid): Interactive Polymers for Tissue Engineering

MRS Proceedings ◽

10.1557/proc-394-77 ◽

1995 ◽

Vol 394 ◽

Cited By ~ 3

Author(s):

Jeffrey S. Hrkach ◽

Jean Ou ◽

Noah Lotan ◽

Robert Langer

Keyword(s):

Tissue Engineering ◽

Lactic Acid ◽

Cell Growth ◽

Aspartic Acid ◽

Biodegradable Polymers ◽

Cell Attachment ◽

Biologically Active ◽

Functional Sites ◽

Support Cell ◽

Enhance Cell Attachment

AbstractOne of the challenges in the field of tissue engineering is the development of optimal materials for use as scaffolds to support cell growth and tissue development. For this purpose, we are developing synthetic, biodegradable polymers with functional sites that provide the opportunity to covalently attach biologically active molecules to the polymers, so they can predictably interact with cells in a favorable manner to enhance cell attachment and growth. The preparation of poly(L-lactic acid-co-aspartic acid) comb-like graft copolymers from poly(L-lactic acid-co-β-benzyl-L-aspartate), and the casting of polymer films by solvent evaporation were carried out.

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Special Issue: Novel Advances and Approaches in Biomedical Materials Based on Calcium Phosphates

Materials ◽

10.3390/ma12030405 ◽

2019 ◽

Vol 12 (3) ◽

pp. 405 ◽

Cited By ~ 4

Author(s):

Michael Mucalo

Keyword(s):

Calcium Phosphate ◽

Cell Attachment ◽

Calcium Phosphates ◽

Wide Spectrum ◽

Special Issue ◽

Dolomitic Marble ◽

Biomedical Materials ◽

Surface Contaminants ◽

Innovative Solutions ◽

Enhance Cell Attachment

Research on calcium phosphate use in the development and clinical application of biomedical materials is a diverse activity and is genuinely interdisciplinary, with much work leading to innovative solutions for improvement of health outcomes. This Special Issue aimed to summarize current advances in this area. The nine papers published cover a wide spectrum of topical areas, such as (1) remineralisation pastes for decalcified teeth, (2) use of statins to enhance bone formation, (3) how dolomitic marble and seashells can be processed into bioceramic materials, (4) relationships between the roughness of calcium phosphate surfaces and surface charge with the effect on human MRC osteogenic differentiation and maturation being investigated, (5) rheological and mechanical properties of a novel injectable bone substitute, (6) improving strength of bone cements by incorporating reinforcing chemically modified fibres, (7) using adipose stem cells to stimulate osteogenesis, osteoinduction, and angiogenesis on calcium phosphates, (8) using glow discharge treatments to remove surface contaminants from biomedical materials to enhance cell attachment and improve bone generation, and (9) a review on how classically brittle hydroxyapatite based scaffolds can be improved by making fibre-hydroxyapatite composites, with detailed analysis of ceramic crack propagation mechanisms and its prevention via fibre incorporation in the hydroxyapatite.

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Selective fluorescence sensing of H2PO4− by the anion induced formation of self-assembled supramolecular polymers

Organic & Biomolecular Chemistry ◽

10.1039/d0ob00258e ◽

2020 ◽

Vol 18 (20) ◽

pp. 3858-3866 ◽

Cited By ~ 4

Author(s):

Paula Sabater ◽

Fabiola Zapata ◽

Adolfo Bastida ◽

Antonio Caballero

Keyword(s):

Halogen Bonding ◽

Supramolecular Polymers ◽

Supramolecular Polymer ◽

Fluorescence Sensing ◽

Tripodal Receptor ◽

Self Assembled

H2PO4− anions induced the formation of a fluorescent supramolecular polymer by halogen bonding interactions in a bromoimidazolium based tripodal receptor.

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Deciphering the potentiometric properties of (porphinato)zinc(ii)-derived supramolecular polymers and related superstructures

Journal of Materials Chemistry C ◽

10.1039/c8tc02979b ◽

2018 ◽

Vol 6 (44) ◽

pp. 11980-11991 ◽

Cited By ~ 7

Author(s):

Chuan Liu ◽

Kaixuan Liu ◽

Jared Klutke ◽

Adam Ashcraft ◽

Samantha Steefel ◽

...

Keyword(s):

Structure Function ◽

Electrochemical Measurement ◽

Measurement Methods ◽

Supramolecular Polymers ◽

Self Assembled

Using electrochemical measurement methods, potentiometric properties of porphyrin-based supramolecular polymers have been investigated. Structure-function relationships of these self-assembled materials have been elucidated.

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Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein

Medicina ◽

10.3390/medicina55100682 ◽

2019 ◽

Vol 55 (10) ◽

pp. 682 ◽

Cited By ~ 1

Author(s):

Sena ◽

Sumeyra ◽

Ulkugul ◽

Sema ◽

Betul ◽

...

Keyword(s):

Cell Attachment ◽

Metformin Hydrochloride ◽

Core Shell ◽

Order Kinetic ◽

Drug Release Profile ◽

Scanning Calorimetry ◽

Electrospinning Technique ◽

Sarcoplasmic Protein ◽

Enhance Cell Attachment ◽

Diabetic Wound Healing

Background and Objectives: A coaxial electrospinning technique was used to produce core/shell nanofibers of a polylactic acid (PLA) as a shell and a polyvinyl alcohol (PVA) containing metformin hydrochloride (MH) as a core. Materials and Methods: Fish sarcoplasmic protein (FSP) was extracted from fresh bonito and incorporated into nanofiber at various concentrations to investigate the influence on properties of the coaxial nanofibers. The morphology, chemical structure and thermal properties of the nanofibers were studied. Results: The results show that uniform and bead-free structured nanofibers with diameters ranging from 621 nm to 681 nm were obtained. A differential scanning calorimetry (DSC) analysis shows that FSP had a reducing effect on the crystallinity of the nanofibers. Furthermore, the drug release profile of electrospun fibers was analyzed using the spectrophotometric method. Conclusions: The nanofibers showed prolonged and sustained release and the first order kinetic seems to be more suitable to describe the release. MTT assay suggests that the produced drug and protein loaded coaxial nanofibers are non-toxic and enhance cell attachment. Thus, these results demonstrate that the produced nanofibers had the potential to be used for diabetic wound healing applications.

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Recent Progress of Synergistic Supramolecular Polymers: Preparation, Properties and Applications

Chemical Communications ◽

10.1039/d0cc07247h ◽

2020 ◽

Author(s):

Yufeng Huo ◽

Zhenfeng He ◽

Chao Wang ◽

Lei Zhang ◽

Qianyu Xuan ◽

...

Keyword(s):

Hydrogen Bonding ◽

Recent Progress ◽

Noncovalent Interactions ◽

The Self ◽

Metal Coordination ◽

Supramolecular Polymers ◽

Π Stacking ◽

Self Assembled

Supramolecular polymers combine the properties of traditional polymers and supramolecules. They are normally formed by the self-assembled polymerization driven via noncovalent interactions (such as hydrogen bonding, π-π stacking, metal coordination,...

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