Poly(L-Lactic Acid-Co-Aspartic Acid): Interactive Polymers for Tissue Engineering

1995 ◽  
Vol 394 ◽  
Author(s):  
Jeffrey S. Hrkach ◽  
Jean Ou ◽  
Noah Lotan ◽  
Robert Langer
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Cell Growth ◽  
Aspartic Acid ◽  
Biodegradable Polymers ◽  
Cell Attachment ◽  
Biologically Active ◽  
Functional Sites ◽  
Support Cell ◽  
Enhance Cell Attachment

AbstractOne of the challenges in the field of tissue engineering is the development of optimal materials for use as scaffolds to support cell growth and tissue development. For this purpose, we are developing synthetic, biodegradable polymers with functional sites that provide the opportunity to covalently attach biologically active molecules to the polymers, so they can predictably interact with cells in a favorable manner to enhance cell attachment and growth. The preparation of poly(L-lactic acid-co-aspartic acid) comb-like graft copolymers from poly(L-lactic acid-co-β-benzyl-L-aspartate), and the casting of polymer films by solvent evaporation were carried out.

scholarly journals HTCC-Modified Nanoclay for Tissue Engineering Applications: A Synergistic Cell Growth and Antibacterial Efficiency

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Majid Aliabadi ◽  
Roya Dastjerdi ◽  
Kourosh Kabiri
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Cell Attachment ◽  
Exchange Process ◽  
Electrospun Nanofibers ◽  
Chronic Infections ◽  
Engineering Applications ◽  
Antibacterial Efficiency ◽  
Modified Clay ◽  
Modified Montmorillonite

This paper deals with the synthesis of a biocompatible chitosan ammonium salt N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) and using it in montmorillonite ion-exchange process. HTCC-modified montmorillonite (Mt) with different chemical ratios was successfully synthesized, and their characteristics have been verified by XRD and FTIR analyses. Produced samples have been evaluated in terms of antibacterial efficiency and biocompatibility (cell culture test). Antibacterial efficiency of synthesized HTCC/Mt samples has been confirmed against both gram negative bacteria (Escherichia coli) and gram positive bacteria (Staphylococcus aureus). The results disclosed that the antibacterial efficiency of HTCC-modified montmorillonite was unexpectedly even more than HTCC. This excellent synergistic effect has been referred to entrapping bacteria between the intercalated structures of HTCC-modified montmorillonite. Then HTCC on clay layers can seriously attack and damage the entrapped bacteria. An extraordinary biocompatibility, cell attachment, and cell growth even more than tissue culture polystyrene (TCPS) have been recorded in the case of this novel kind of modified clay. Due to existing concerns about serious and chronic infections after implant placement, this natural-based bioactive and antibacterial modified clay can be used in electrospun nanofibers and other polymeric implants with promising mechanical properties for tissue engineering applications.

Electrospun Scaffolds Composed of Poly(L-lactic acid) and Hydroxyapatite

2011 ◽  
Vol 493-494 ◽  
pp. 872-877 ◽  
Author(s):  
G.N.P Rodriguez ◽  
L.R. Rodrigues ◽  
C.G.B.T. Dias ◽  
M.A. d´Ávila ◽  
C.A.C. Zavaglia
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Cell Attachment ◽  
Polymeric Materials ◽  
Porous Membranes ◽  
Electrospun Scaffolds ◽  
Bioactive Ceramic ◽  
Biomedical Field ◽  
Fibrous Membranes ◽  
Artificial Extracellular Matrix

Tissue engineering is an important emerging area for creating biological alternatives for harvested tissues, implants, and prostheses. Biocompatible and biodegradable polymeric materials are considered an important class of materials that can be used as scaffolds in tissue engineering applications. In this work, the system studied was nanocomposites of hydroxyapatite (HA) dispersed in a matrix of PLLA. Scaffolds have to present similar structure and also function as an artificial extracellular matrix for cell attachment and growth. Hydroxyapatite is a bioactive ceramic and has been used in applications of repairing bone tissue due to its biocompatibility and osteoconductivity. Poly(L- lactic acid) is a biodegradable and biocompatible polymer and has been used in different applications in the biomedical field. In this work, polymer solutions were prepared with different percentages of hydroxyapatite and porous membranes consisting of non-woven nanostructured fibers were obtained by electrospinning. The process parameters were: voltage of 13kV, flow rate of 0.5 ml/h and distance from the tip of the needle to the collector of 12 cm. By using these process parameter, fibrous membranes were obtained with different concentrations of HA (1.96, 4.76, 9 [wt %]). The morphology of the samples was observed by SEM and the characteristic physic-chemical were analyzed by XRF, XRD, DSC and FTIR.

Surface modification of poly(l-lactic acid) affects initial cell attachment, cell morphology, and cell growth

2005 ◽  
Vol 7 (4) ◽  
pp. 187-193 ◽  
Author(s):  
Manae Yamaguchi ◽  
Toshio Shinbo ◽  
Toshiyuki Kanamori ◽  
Pi-chao Wang ◽  
Motohiro Niwa ◽  
...  
Keyword(s):  
Surface Modification ◽  
Lactic Acid ◽  
Cell Growth ◽  
Cell Morphology ◽  
Cell Attachment ◽  
Initial Cell

Surface Engineering of Nano-fibrous Biodegradable Poly(L-lactic acid) Scaffolds for Tissue Engineering

2004 ◽  
Vol 823 ◽  
Author(s):  
Xiaohua Liu ◽  
Youngjun Won ◽  
Peter X. Ma
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Surface Engineering ◽  
Surface Composition ◽  
Cell Attachment ◽  
Pore Wall ◽  
Porous Scaffolds ◽  
Cell Numbers ◽  
Fibrous Scaffolds ◽  
Surface Modified

AbstractThe architectural design and surface properties of scaffolds are important aspects in tissue engineering. The porous scaffolds accommodate cells and guide their growth, while the surface nature of the scaffolds can directly affect cell attachment, proliferation, and ultimately neo tissue regeneration. In this work, a highly porous poly(L-lactic acid) (PLLA) scaffold with nano-fibrous pore wall architecture has been fabricated by mimicking the structure of natural collagen using a novel thermally induced phase separation method developed in our group. A universally effective surface modification method was developed, and gelatin was successfully grafted onto the surface of nano-fibrous PLLA scaffolds by entrapment procedure. The surface composition, morphology, and properties were examined using ATR-FTIR, XPS and SEM. The surface coverage of gelatin on the PLLA surface was as high as 39.4%. MC3T3-E1 osteoprogenitor cells were cultured for 6 weeks in solid-walled PLLA scaffolds, nano-fibrous PLLA scaffolds, and surface-modified nano-fibrous PLLA scaffolds, respectively. The osteoblasts proliferated in all three types of scaffolds, but the cell numbers were always significantly higher in the surface-modified nano-fibrous scaffolds than in the other two types of scaffolds, and the cell numbers in nano-fibrous scaffolds were higher than that in the solid-walled scaffolds. These results demonstrate that the surface-modified nano-fibrous architecture could serve as a superior scaffold for tissue engineering.

scholarly journals Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

2015 ◽  
Vol 12 (108) ◽  
pp. 20150254 ◽  
Author(s):  
Marwa Tallawi ◽  
Elisabetta Rosellini ◽  
Niccoletta Barbani ◽  
Maria Grazia Cascone ◽  
Ranjana Rai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Cell Attachment ◽  
Polyglycolic Acid ◽  
Synthetic Polymers ◽  
Cardiac Tissue Engineering ◽  
Biomaterial Surface ◽  
Enhance Cell Attachment ◽  
Cardiac Patch ◽  
Material Interaction

The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers (polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly- l -lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

scholarly journals The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications

2021 ◽  
Vol 22 (6) ◽  
pp. 3086
Author(s):  
Stefanos Kikionis ◽  
Efstathia Ioannou ◽  
Eleni Aggelidou ◽  
Leto-Aikaterini Tziveleka ◽  
Efterpi Demiri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Hybrid Composites ◽  
Sulfated Polysaccharide ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Natural Polymers ◽  
The Impact

Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering. Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties. Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized. The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells. The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.

Development of Cartilage and Bone During Formation of Phalanges by Tissue Engineering

1999 ◽  
Vol 5 (S2) ◽  
pp. 378-379
Author(s):  
W. Landis ◽  
N. Isogai ◽  
J. Vacanti
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Congenital Malformations ◽  
Flat Surface ◽  
Bone Cells ◽  
Cell Attachment ◽  
Organ Function ◽  
Tendon Cells ◽  
Specific Shape ◽  
Different Sources

Treatment of human congenital malformations and for loss of tissue or organ function is a serious clinical concern, frequently supported by harvesting autogenous tissue for reconstructive surgery. This paper summarizes an alternate approach through novel tissue engineering methods and describes cell-polymer composites that could be fundamental in developing advances for digit and joint replacement. The work utilizes cells from different sources transplanted on biodegradable polymer matrices to form structures with defined shape, morphological features and composition resembling those of human phalanges and intact joints.Polyglycolic acids (PGA) fibers (Albany International, Mansfield, MA) 15 μm in diameter were used as a mesh support for cell attachment and matrix formation. For cartilage and tendon cells, PGA alone served as a flat surface without specific shape. For bone cells, PGA was copolymerized with poly-L-lactic acid (PLLA) (Polysciences, Warrington, PA) and shaped after a human phalanx molded from cadaveric distal and middle phalangeal bones. Polymers were sterilized with ethylene oxide before use.

Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

2007 ◽  
Vol 1025 ◽  
Author(s):  
Juana Mendenhall ◽  
Dapeng Li ◽  
Margaret Frey ◽  
Juan Hinestroza ◽  
Omotunde Babalola ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Surface Topography ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Coverage Area ◽  
Surface Initiated Polymerization ◽  
Natural Tissue

AbstractThree dimensional scaffolds (3D) are promising for future nanoscale materials and tissue engineering applications being that they have architecture and mechanical properties similar to natural tissue. In this work, poly(lactic acid) fibers were prepared via electrospinnig with average diameters of 2580 nm. Using Enzymatic Surface-Initiated polymerization (ESIP), poly(3-hydroxybutyrate) were coated on poly(lactic acid) fibers. This provides an alternative method to enzymatic surface modification of fibers. ESIP of PHB produces a granular film providing surface topography and increases mechanical properties of PLA fibers alone. When using covalent approaches, PHB granules provide surface topography of 200-500 nm with a polydisperse coverage area. Compressive modulus measurements of PLA and PHB/PLA scaffolds were 25 kPa and 73 kPa, respectively. The percent crystallinity of PLA and PHB/PLA scaffolds was 17% and 30%, respectively. This rough topography, in addition to the crystallinity of the scaffold, facilitates Soas-2 osteoblast cell attachment. We have observed attachment of the osteoblast cells along the length of the oriented PLA and PHB/PLA composite scaffolds with different morphologies, rounded and stretched, throughout a depth of 90 µm.

scholarly journals Investigation on the printability of bioink based on alginate-gelatin hydrogel and liquid crystals

2020 ◽  
Vol 9 (4) ◽  
pp. 1718-1725
Author(s):  
Alyaa Idrees Abdulmaged ◽  
Chin Fhong Soon ◽  
Balkis A. Talip ◽  
Sheril Amira Othman ◽  
Gim Pao Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liquid Crystals ◽  
Flow Rate ◽  
Potential Application ◽  
Cell Attachment ◽  
3D Bioprinting ◽  
Minimum Width ◽  
Gelatin Hydrogel ◽  
Support Cell ◽  
Gelatin Concentration

Bioinks of 3D bioprinting have significant potential application in the field of tissue engineering to support cell attachment and proliferation. In this work, the alginate-gelatin-CELC (AGLC) bioink based on different compositions of alginate-gelatin (AG) hydrogel and cholesteryl ester liquid crystals (CELC) was prepared. Primarily, the alginate-gelatin hydrogel with certain concentration of Gelatin (10-50%w/v) was investigated. The printability of the hydrogel reached a minimum width of 1.8 mm at a flow rate of 1 mL/min when the Gelatin concentration was increased to 50 % w/v (AG1050). Subsequently, the respective polymers with 10% w/v Alginate and50% w/v Gelatin blended with 1%, 5%, 10%, 20%, 40%, and 60% w/v of CELC in the preparation of the alginate-gelatin-CELC bioink was further investigated. The printability of the bioink was examined by micro-extrusion based 3D bioprinter. The printability of the bioink enhanced by 27.8% as compared to AG1050 and reached a minimum width of 1.3 mm at a flow rate of 1 mL/min when the CELC concentration was increased to 40% and 60%. The tested properties of the bioink show that the CELC improve shear-thinning and lipid moieties properties to the composite bioink and hence, enhances its printability.

Efficient in situ gene delivery via PEG diacrylate matrices

2018 ◽  
Vol 6 (12) ◽  
pp. 3241-3250 ◽  
Author(s):  
Paresh Shrimali ◽  
Mathew Peter ◽  
Archana Singh ◽  
Neha Dalal ◽  
Suresh Dakave ◽  
...  
Keyword(s):  
Cell Growth ◽  
Gene Delivery ◽  
Cell Attachment ◽  
Support Cell ◽  
Virus Retention

Cryogels of PEGDA support cell growth, and those of PEGDA–GELMA allow only cell attachment but no virus retention while those of PEGDA–PLL enhance transduction via the colocalization of cells and viruses.

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