721: Combined non-invasive bioluminiscence and magnetic resonance imaging improves detection after pulmonary gene transfer in a fetal mouse model

Abstract Magnetic resonance imaging (MRI) is a promising diagnostic method for evaluation of bone marrow myelofibrosis for offering non-invasive, wider-area coverage over conventional histology. Although the most striking feature in histology of myelofibrotic bone marrow is the proliferation of reticulin fibers, the spectrum of findings involves abnormalities in cell number and composition, vessel proliferation, as well as possible pre-fibrotic abnormalities in matrix such as inflammation/edema. Correlation of MRI observations with such histological findings to accurately define the pathogenic phases of myelofibrosis has been challenging. Here, we present the first MRI analysis of myelofibrosis in a mouse model (Gata-1low mice) in an attempt to correlate MRI signal observations with histological findings. T2-weighted MRI images of femur bone marrow of Gata-1low animals at different ages (12 to 46 weeks) showed high MR signal intensity, absent in age-matched wild-type controls, that is detected as early as 12 weeks of age, before onset of histologically-defined fibrosis in this mouse model. MRI acquisitions employing fat suppression, a technique that suppresses signals from lipids, together with the absence of chemical shift artifacts in the images, excluded the possibility of lipids as the source of the prominent MR signal. To determine the source of this prominent non-lipid signal in Gata-1low bone marrow, cellular composition, vascularization and vascular permeability were tested. Our findings demonstrate a multi-source nature of MRI signals in bone marrow, which evolves from young (12 weeks) to old (40 weeks) Gata-1low bone marrow consistent with the pathologic progression of myelofibrosis. Data support the importance of an integrative approach to further the development of this non-invasive modality for following the dynamics and progression of this multifactorial pathology in humans. Disclosures No relevant conflicts of interest to declare.

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A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI)

Clinical Immunology ◽

10.1016/j.clim.2016.07.013 ◽

2016 ◽

Vol 172 ◽

pp. 52-60 ◽

Cited By ~ 4

Author(s):

Francesca Valdora ◽

Giovanna Cutrona ◽

Serena Matis ◽

Fortunato Morabito ◽

Carlotta Massucco ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Iron Oxide ◽

Magnetic Resonance ◽

Chronic Lymphocytic Leukemia ◽

Mouse Model ◽

Superparamagnetic Iron Oxide ◽

Lymphocytic Leukemia ◽

Resonance Imaging ◽

Invasive Approach ◽

Non Invasive

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Safety and Practicality of Magnetic Resonance Imaging (MRI) for Non-invasive Assessment of Coronary Stents

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(97)85365-x ◽

1998 ◽

Vol 31 (2) ◽

pp. 382A

Author(s):

S Rosario

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Coronary Stents ◽

Resonance Imaging ◽

Non Invasive ◽

Magnetic Resonance Imaging Mri

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Faculty Opinions recommendation of Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1020421.232636 ◽

2004 ◽

Author(s):

Angela Vincent

Keyword(s):

Magnetic Resonance Imaging ◽

Multiple Sclerosis ◽

T Cells ◽

Magnetic Resonance ◽

Mouse Model ◽

Resonance Imaging

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Non-invasive diagnostics in fossils - Magnetic Resonance Imaging of pathological belemnites

Biogeosciences ◽

10.5194/bg-2-133-2005 ◽

2005 ◽

Vol 2 (2) ◽

pp. 133-140 ◽

Cited By ~ 18

Author(s):

D. Mietchen ◽

H. Keupp ◽

B. Manz ◽

F. Volke

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Soft Tissue ◽

Clinical Diagnostics ◽

Resonance Imaging ◽

Non Invasive ◽

Physiological Processes ◽

Magnetic Resonance Imaging Mri ◽

Invasive Diagnostics

Abstract. For more than a decade, Magnetic Resonance Imaging (MRI) has been routinely employed in clinical diagnostics because it allows non-invasive studies of anatomical structures and physiological processes in vivo and to differentiate between healthy and pathological states, particularly of soft tissue. Here, we demonstrate that MRI can likewise be applied to fossilized biological samples and help in elucidating paleopathological and paleoecological questions: Five anomalous guards of Jurassic and Cretaceous belemnites are presented along with putative paleopathological diagnoses directly derived from 3D MR images with microscopic resolution. Syn vivo deformities of both the mineralized internal rostrum and the surrounding former soft tissue can be traced back in part to traumatic events of predator-prey-interactions, and partly to parasitism. Besides, evidence is presented that the frequently observed anomalous apical collar might be indicative of an inflammatory disease. These findings highlight the potential of Magnetic Resonance techniques for further paleontological applications.

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Multi-modal magnetic resonance imaging in a mouse model of concussion

Scientific Data ◽

10.1038/s41597-021-00985-w ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Xuan Vinh To ◽

Fatima A. Nasrallah

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Data Collection ◽

Mouse Model ◽

Meta Analysis ◽

Data Repository ◽

Resonance Imaging ◽

Loss Of Righting Reflex ◽

Post Impact ◽

Magnetic Resonance Imaging Mri

AbstractThis data collection contains Magnetic Resonance Imaging (MRI) data, including structural, diffusion, stimulus-evoked, and resting-state functional MRI and behavioural assessment results, including acute post-impact Loss-of-Righting Reflex time and acute, subacute, and longer-term Neural Severity Score, and Open Field Behaviour obtained from a mouse model of concussion. Four cohorts with 43 3–4 months old male mice in total were used: Sham (n = 14, n = 6 day 2, n = 3 day 7, n = 5 day 14), concussion day 2 (CON 2; n = 9), concussion day 7 (CON 7; n = 10), concussion day 14 (CON 14; n = 10). The data collection contains the aforementioned MRI data in compressed NIFTI format, data sheets on animal’s backgrounds and behavioural outcomes and is made publicly available from a data repository. The available data are intended to facility cross-study comparisons, meta-analysis, and science reproducibility.

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Demyelination and remyelination detected in an alternative cuprizone mouse model of multiple sclerosis with 7.0 T multiparameter magnetic resonance imaging

Scientific Reports ◽

10.1038/s41598-021-90597-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shuang Ding ◽

Yu Guo ◽

Xiaoya Chen ◽

Silin Du ◽

Yongliang Han ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Multiple Sclerosis ◽

Magnetic Resonance ◽

Mouse Model ◽

Diffusion Tensor ◽

Mean Diffusivity ◽

Blue Staining ◽

Resonance Imaging ◽

The Mean ◽

Underlying Mechanisms

AbstractThe aim of this study was to investigate the mechanisms underlying demyelination and remyelination with 7.0 T multiparameter magnetic resonance imaging (MRI) in an alternative cuprizone (CPZ) mouse model of multiple sclerosis (MS). Sixty mice were divided into six groups (n = 10, each), and these groups were imaged with 7.0 T multiparameter MRI and treated with an alternative CPZ administration schedule. T2-weighted imaging (T2WI), susceptibility-weighted imaging (SWI), and diffusion tensor imaging (DTI) were used to compare the splenium of the corpus callosum (sCC) among the groups. Prussian blue and Luxol fast blue staining were performed to assess pathology. The correlations of the mean grayscale value (mGSV) of the pathology results and the MRI metrics were analyzed to evaluate the multiparameter MRI results. One-way ANOVA and post hoc comparison showed that the normalized T2WI (T2-nor), fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) values were significantly different among the six groups, while the mean phase (Φ) value of SWI was not significantly different among the groups. Correlation analysis showed that the correlation between the T2-nor and mGSV was higher than that among the other values. The correlations among the FA, RD, MD, and mGSV remained instructive. In conclusion, ultrahigh-field multiparameter MRI can reflect the pathological changes associated with and the underlying mechanisms of demyelination and remyelination in MS after the successful establishment of an acute CPZ-induced model.

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