Adaptive Laboratory Evolution for algal strain improvement: methodologies and applications

2020 ◽  
pp. 102122
Author(s):  
Alaina J. LaPanse ◽  
Anagha Krishnan ◽  
Matthew C. Posewitz
Keyword(s):  
Strain Improvement ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Algal Strain ◽  
Improvement Methodologies

scholarly journals Application of Microalgal Stress Responses in Industrial Microalgal Production Systems

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 30
Author(s):  
Jia Wang ◽  
Yuxin Wang ◽  
Yijian Wu ◽  
Yuwei Fan ◽  
Changliang Zhu ◽  
...  
Keyword(s):  
Stress Responses ◽  
Production Systems ◽  
Strain Improvement ◽  
Value Added ◽  
Product Yield ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Theoretical Support ◽  
Further Development ◽  
Value Added Products

Adaptive laboratory evolution (ALE) has been widely utilized as a tool for developing new biological and phenotypic functions to explore strain improvement for microalgal production. Specifically, ALE has been utilized to evolve strains to better adapt to defined conditions. It has become a new solution to improve the performance of strains in microalgae biotechnology. This review mainly summarizes the key results from recent microalgal ALE studies in industrial production. ALE designed for improving cell growth rate, product yield, environmental tolerance and wastewater treatment is discussed to exploit microalgae in various applications. Further development of ALE is proposed, to provide theoretical support for producing the high value-added products from microalgal production.

Strain improvement of Chlorella sp. for phenol biodegradation by adaptive laboratory evolution

2016 ◽  
Vol 205 ◽  
pp. 264-268 ◽  
Author(s):  
Libo Wang ◽  
Chuizhao Xue ◽  
Liang Wang ◽  
Quanyu Zhao ◽  
Wei Wei ◽  
...  
Keyword(s):  
Strain Improvement ◽  
Adaptive Laboratory Evolution ◽  
Phenol Biodegradation ◽  
Laboratory Evolution ◽  
Chlorella Sp

scholarly journals Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

2020 ◽  
Vol 12 (12) ◽  
pp. 5125
Author(s):  
Neha Arora ◽  
Hong-Wei Yen ◽  
George P. Philippidis
Keyword(s):  
Genetic Engineering ◽  
Lipid Production ◽  
Random Mutagenesis ◽  
Strain Improvement ◽  
Production Costs ◽  
Screening Methods ◽  
Scale Production ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Oleaginous Microalgae

Oleaginous microalgae and yeasts represent promising candidates for large-scale production of lipids, which can be utilized for production of drop-in biofuels, nutraceuticals, pigments, and cosmetics. However, low lipid productivity and costly downstream processing continue to hamper the commercial deployment of oleaginous microorganisms. Strain improvement can play an essential role in the development of such industrial microorganisms by increasing lipid production and hence reducing production costs. The main means of strain improvement are random mutagenesis, adaptive laboratory evolution (ALE), and rational genetic engineering. Among these, random mutagenesis and ALE are straight forward, low-cost, and do not require thorough knowledge of the microorganism’s genetic composition. This paper reviews available mutagenesis and ALE techniques and screening methods to effectively select for oleaginous microalgae and yeasts with enhanced lipid yield and understand the alterations caused to metabolic pathways, which could subsequently serve as the basis for further targeted genetic engineering.

scholarly journals Adaptive laboratory evolution of β-caryophyllene producing Saccharomyces cerevisiae

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Avinash Godara ◽  
Katy C. Kao
Keyword(s):  
Oxidative Stress ◽  
Yeast Strain ◽  
Strain Improvement ◽  
Genomic Analysis ◽  
Pathway Engineering ◽  
Selection Strategy ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Fourfold Increase ◽  
Extracellular Product

Abstract Background β-Caryophyllene is a plant terpenoid with therapeutic and biofuel properties. Production of terpenoids through microbial cells is a potentially sustainable alternative for production. Adaptive laboratory evolution is a complementary technique to metabolic engineering for strain improvement, if the product-of-interest is coupled with growth. Here we use a combination of pathway engineering and adaptive laboratory evolution to improve the production of β-caryophyllene, an extracellular product, by leveraging the antioxidant potential of the compound. Results Using oxidative stress as selective pressure, we developed an adaptive laboratory evolution that worked to evolve an engineered β-caryophyllene producing yeast strain for improved production within a few generations. This strategy resulted in fourfold increase in production in isolated mutants. Further increasing the flux to β-caryophyllene in the best evolved mutant achieved a titer of 104.7 ± 6.2 mg/L product. Genomic analysis revealed a gain-of-function mutation in the a-factor exporter STE6 was identified to be involved in significantly increased production, likely as a result of increased product export. Conclusion An optimized selection strategy based on oxidative stress was developed to improve the production of the extracellular product β-caryophyllene in an engineered yeast strain. Application of the selection strategy in adaptive laboratory evolution resulted in mutants with significantly increased production and identification of novel responsible mutations.

scholarly journals Engineering improved ethylene production: Leveraging systems Biology and adaptive laboratory evolution

2021 ◽  
Author(s):  
Sophie Vaud ◽  
Nicole Pearcy ◽  
Marko Hanževački ◽  
Alexander M.W. Van Hagen ◽  
Salah Abdelrazig ◽  
...  
Keyword(s):  
Systems Biology ◽  
Ethylene Production ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution

scholarly journals Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol

2019 ◽  
Vol 20 (22) ◽  
pp. 5737 ◽  
Author(s):  
Miriam González-Villanueva ◽  
Hemanshi Galaiya ◽  
Paul Staniland ◽  
Jessica Staniland ◽  
Ian Savill ◽  
...  
Keyword(s):  
Type Strain ◽  
Wild Type Strain ◽  
Carbon Sources ◽  
Strain Engineering ◽  
Cupriavidus Necator ◽  
Superior Performance ◽  
Wild Type ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Cupriavidus Necator H16

Cupriavidus necator H16 is a non-pathogenic Gram-negative betaproteobacterium that can utilize a broad range of renewable heterotrophic resources to produce chemicals ranging from polyhydroxybutyrate (biopolymer) to alcohols, alkanes, and alkenes. However, C. necator H16 utilizes carbon sources to different efficiency, for example its growth in glycerol is 11.4 times slower than a favorable substrate like gluconate. This work used adaptive laboratory evolution to enhance the glycerol assimilation in C. necator H16 and identified a variant (v6C6) that can co-utilize gluconate and glycerol. The v6C6 variant has a specific growth rate in glycerol 9.5 times faster than the wild-type strain and grows faster in mixed gluconate–glycerol carbon sources compared to gluconate alone. It also accumulated more PHB when cultivated in glycerol medium compared to gluconate medium while the inverse is true for the wild-type strain. Through genome sequencing and expression studies, glycerol kinase was identified as the key enzyme for its improved glycerol utilization. The superior performance of v6C6 in assimilating pure glycerol was extended to crude glycerol (sweetwater) from an industrial fat splitting process. These results highlight the robustness of adaptive laboratory evolution for strain engineering and the versatility and potential of C. necator H16 for industrial waste glycerol valorization.

scholarly journals ALEdb 1.0: a database of mutations from adaptive laboratory evolution experimentation

2018 ◽  
Vol 47 (D1) ◽  
pp. D1164-D1171 ◽  
Author(s):  
Patrick V Phaneuf ◽  
Dennis Gosting ◽  
Bernhard O Palsson ◽  
Adam M Feist
Keyword(s):  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution
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