Apelin and apelin receptor at different stages of corpus luteum development and effect of apelin on progesterone secretion and 3β-hydroxysteroid dehydrogenase (3β-HSD) in pigs

2018 ◽  
Vol 192 ◽  
pp. 251-260 ◽  
Author(s):  
Marta Różycka ◽  
Patrycja Kurowska ◽  
Małgorzata Grzesiak ◽  
Małgorzata Kotula-Balak ◽  
Wacław Tworzydło ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Hydroxysteroid Dehydrogenase ◽  
Progesterone Secretion ◽  
Apelin Receptor

scholarly journals Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro

2001 ◽  
Vol 49 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Grażyna Miszkiel ◽  
J. Kotwica
Keyword(s):  
Corpus Luteum ◽  
Mechanism Of Action ◽  
Hydroxysteroid Dehydrogenase ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis ◽  
Luteal Tissue ◽  
Prostaglandin F

The present studies were conducted: (1) to determine which β-adrenoceptor subtypes are involved in progesterone and oxytocin (OT) secretion, (2) to examine whether noradrenaline (NA) acts directly on the cytochrome P-450scc and 3β-hydroxysteroid dehydrogenase (3β-HSD), and (3) to study the effect of prostaglandin F2α, (PGF2α) on NA-stimulated steroidogenesis in luteal cells. The effect of NA on progesterone secretion from luteal slices of heifers on days 8–12 of the oestrous cycle was blocked by both atenolol (β1-antagonist) and ICI 118.551 hydrochloride (β2-antagonist). OT secretion was blocked only after treatment with ICI 118.551 hydrochloride (P < 0.05). Dobutamine (10−4−10−6), a selective β1 agonist and salbutamol (10−4−10−6), a selective β2 agonist, both increased progesterone production (P < 0.01) with an efficiency comparable to that produced by NA (P < 0.01). The increase of OT content in luteal slices was observed only after treatment with salbutamol at the dose of 10−5M (P < 0.01). Dobutamine had no effect on OT production at any dose. A stimulatory effect of NA on cytochrome P-450scc activity (P < 0.05) was demonstrated using 25-hydroxycholesterol as substrate. 3β-HSD activity also increased following NA (P < 0.01) or pregnenolone (P < 0.05) and in tissue treated with pregnenolone together with NA (P < 0.01). PGF decreased progesterone synthesis (P < 0.05) and 3β-HSD activity (P < 0.01) in tissue treated with NA. We conclude that NA stimulates progesterone secretion by luteal β1- and β2-adrenoceptors, while OT secretion is probably mediated only via the β2-receptor. NA also increases cytochrome P-450scc and 3β-HSD activity. PGF inhibits the luteotropic effect of NA on the luteal tissue.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Local progesterone secretion by the marmoset corpus luteum after perfusion with regulating hormones

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S111-S112 ◽  
Author(s):  
G.E. WEBLEY ◽  
J.P. HEARN ◽  
M.R. LUCK
Keyword(s):  
Corpus Luteum ◽  
Progesterone Secretion

Pulsatile secretion of progesterone from the human corpus luteum: poor correlation with bioactive LH pulses

1986 ◽  
Vol 111 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Inese Z. Beitins ◽  
Maria L. Dufau
Keyword(s):  
Corpus Luteum ◽  
Temporal Relationship ◽  
Pulse Amplitude ◽  
Pulse Frequency ◽  
Biologically Active ◽  
Poor Correlation ◽  
Serum Progesterone ◽  
Progesterone Secretion ◽  
Wide Range ◽  
Human Corpus Luteum

Abstract. Having previously established that biologically active luteinizing hormone (LH) is secreted in episodic pulsations that vary in relation to the menstrual cycle, we investigated the possibility that a temporal relationship could exist between the bioactive LH pulses and progesterone secretion from the late corpus luteum. In 4 young women blood was withdrawn every 15 min for 8 h. Serum progesterone concentrations fluctuated at a mean frequency of 0.9 h with a wide range of amplitudes (13.8 to 1.7 ng/ml). Serum bioactive LH pulse frequency in contrast was 0.25 pulses/h in all subjects. The pulse amplitude was 18.2 to 12.4 mIU/ml (2nd IRP-hMG). These data reveal that within the 8 h-period studied, progesterone secretory pulses occurred four times more frequently as those for bioactive LH. Therefore it is unlikely that a temporal relationship exists between individual bioactive LH and pulses of progesterone secreted by the late corpus luteum.

scholarly journals Hubungan Kadar Progesteron pada Fase Awal Luteal dengan Kematian Embrio pada Sapi Aceh

2019 ◽  
Vol 19 (2) ◽  
pp. 107-112
Author(s):  
Budianto Panjaitan ◽  
Citra Chyntia Helwana ◽  
Nellita Meutia ◽  
Yusmadi Yusmadi ◽  
Tongku Nizwan Siregar ◽  
...  
Keyword(s):  
Body Weight ◽  
Corpus Luteum ◽  
Adult Female ◽  
Artificial Insemination ◽  
Luteal Phase ◽  
Embryonic Mortality ◽  
Progesterone Concentration ◽  
Double Injection ◽  
Progesterone Secretion ◽  
Embryonic Death

ABSTRAK.  Progesteron merupakan hormon yang berperan penting dalam proses pemeliharaan kebuntingan dan dihasilkan oleh corpus luteum. Penelitian ini bertujuan mengetahui hubungan antara kadar hormon progesteron pada fase awal luteal dengan kematian embrio pada sapi Aceh. Dalam penelitian ini digunakan empat ekor sapi betina dewasa berumur 3-5 tahun, bobot badan 150-250 kg, sehat secara klinis, dan memiliki reproduksi normal. Sapi disinkronisasi menggunakan 5 ml prostaglandin F2 alfa (PGF2α) dengan pola penyuntikan ganda berinterval 11 hari. Koleksi sampel darah untuk pengukuran konsentrasi progesteron dilakukan pada hari ke-5, 6, dan 7 pasca inseminasi. Pengukuran konsentrasi progesteron dilakukan menggunakan metode enzymelinked-immunoassay (ELISA), pemeriksaan kebuntingan dan kematian embrio menggunakan metode transrektal ultrasonografi pada hari ke-25 pasca inseminasi. Pemeriksaan diulang setiap 10 hari sampai hari ke-55 pasca inseminasi. Puncak sekresi progesteron pada sapi bunting dengan embrio yang bertahan hidup terdapat pada hari ke-7 (2,082 ng/ml), pada sapi Late Embryonic Mortality (LEM) di hari ke-5 (8,209 ng/ml) dan pada sapi tidak bunting di hari ke-7 (3,051±1,157 ng/ml). Sekresi progesteron sapi LEM pada hari ke-5 sampai dengan ke-7 cenderung menurun sedangkan pada sapi yang bertahan hidup cenderung meningkat.  (Correlation between progesterone levels in early luteal phase and embryonic death  in Aceh cattle) ABSTRACT. Progesterone is an important hormone that functions to maintain pregnancy and is produced by the corpus luteum. The aim of this study was to see a correlation between progesterone and the incidence of embryonic death in Aceh cattle. This study used four adult female cows, 3-5 years old, 150-250 kg body weight, clinically healthy, and have a normal reproduction. The synchronized with 5 ml prostaglandin F2 alfa hormone, and double injection pattern with 11-day intervals. The blood was collected for progesterone measurements on 5th, 6th, 7th day post artificial insemination. Measurement of progesterone concentration was carried out using an enzymelinked-immunoassay (ELISA), while pregnancy and embryo mortality was performed using the trans-rectal ultrasonography method on the 25th day after insemination. The examination was repeated every 10 days until day 55th after insemination. Progesterone secretion peaks in pregnant cows were on day 7th (2.082 ng/ml), in cattle Late Embryonic Mortality (LEM) on day 5th (8.209 ng/ml) and in cattle not pregnant on day 7th (3.051±1.157 ng/ml). The pattern of LEM progesterone secretion on days 5th to 7th tends to decrease while those that survive tend to increase.

CYCLISCHE SCHWANKUNGEN DER AKTIVITÄTEN VON HYDROXYSTEROID-DEHYDROGENASEN IM CORPUS LUTEUM DES RINDES

1972 ◽  
Vol 69 (2) ◽  
pp. 369-384
Author(s):  
H. Brandau ◽  
L. Brandau ◽  
G. Mutzke
Keyword(s):  
Enzyme Activity ◽  
Corpus Luteum ◽  
Enzyme Activities ◽  
Optical Method ◽  
Activity Patterns ◽  
Hydroxysteroid Dehydrogenase ◽  
Corpora Lutea ◽  
Bovine Corpus Luteum ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT In the bovine corpora lutea periodical activities of the Δ53β-, 3β-, 17β-and 20β-hydroxysteroid dehydrogenase (OHSDH) as well as activities of glyceraldehyde-3-phosphate- and glucose-6-phosphate dehydrogenase were measured quantitatively and the alterations throughout the different stages of the cycle were studied. After homogenization of the tissue and fractionate centrifugation the enzyme activities were determined by a standardized optical method. The activities of the Δ53β-, and 3β- and 17β-OHSDH increase slowly during the first 7 days of the cycle, the maximum is reached abruptly on the 12th to 13th day of the cycle. After a striking reduction the activities decline continually to the 19th to 21st day reaching the values detected at the beginning of the cycle. The 20β-OHSDH increases slowly to the maximum on the 15th day of the cycle. Activities of the 3α-OHSDH were obtained only inconsistently. The behaviour of the activities of G6PDH was nearly identical with that of the 3β-OHSDH, while the GAPDH shows only little fluctuations of its activities. The obtained enzyme activity patterns of the maturating and high functional corpus luteum correspond to the well-known data of the biosynthetic function of the bovine corpus luteum. The changes of the amounts of progesterone and 20β-progesterol agree with the course of the activities of the 3β- resp. 20β-OHSDH.

Sources of progesterone during gestation in Barbary sheep (Ammotragus lervia)

1983 ◽  
Vol 98 (2) ◽  
pp. 283-NP ◽  
Author(s):  
A. P. F. Flint ◽  
R. D. Burton ◽  
R. B. Heap
Keyword(s):  
Chromosome Number ◽  
Corpus Luteum ◽  
Late Pregnancy ◽  
Hydroxysteroid Dehydrogenase ◽  
Point Of View ◽  
Diploid Chromosome Number ◽  
Domestic Sheep ◽  
Ammotragus Lervia ◽  
Barbary Sheep ◽  
Venous Plasma

Concentrations of progesterone in arterial and ovarian, uterine and jugular venous plasma were determined in four Barbary sheep at various stages of pregnancy. The results, together with ovarian histology, show that the corpus luteum regresses before term in Barbary sheep, as in most breeds of domestic ewes. Uterine synthesis of progesterone was demonstrated in late pregnancy in two animals in which uterine venous levels of progesterone were increased two- to fourfold above arterial concentrations. The placenta contained 3β-hydroxysteroid dehydrogenase. Barbary sheep (diploid chromosome number, 2N = 58) therefore resemble the domestic sheep (2N = 54) rather than the goat (2N = 60) from the point of view of the source of the progesterone required for maintenance of pregnancy.

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