Bacterial endophytes of sweet potato tuberous roots affected by the plant genotype and growth stage

2015 ◽  
Vol 96 ◽  
pp. 273-281 ◽  
Author(s):  
Joana Montezano Marques ◽  
Thais Freitas da Silva ◽  
Renata Estebanez Vollú ◽  
Jackeline Rossetti Mateus de Lacerda ◽  
Arie Fitzgerald Blank ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Growth Stage ◽  
Bacterial Endophytes ◽  
Plant Genotype ◽  
Tuberous Roots

MYB44 competitively inhibits the formation of the MYB340-bHLH2-NAC56 complex to regulate anthocyanin biosynthesis in purple-fleshed sweet potato

2020 ◽  
Author(s):  
Zeng-Zheng Wei ◽  
Kang-Di Hu ◽  
Dong-Lan Zhao ◽  
Jun Tang ◽  
Zhong-Qin Huang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Regulatory Network ◽  
Anthocyanin Biosynthesis ◽  
Expression System ◽  
Firefly Luciferase ◽  
Anthocyanin Accumulation ◽  
Tuberous Roots ◽  
Regulatory Complex ◽  
Transcriptional Complex ◽  
Insight Into

Abstract Background: Anthocyanins, which have important biological functions and have a beneficial effect on human health, notably account for pigmentation in purple-fleshed sweet potato tuberous roots. Individual regulatory factors of anthocyanin biosynthesis have been identified; however, the regulatory network of anthocyanin biosynthesis in purple-fleshed sweet potato is unclear. Results: We functionally determined that IbMYB340 cotransformed with IbbHLH2 in tobacco and strawberry receptacles induced anthocyanin accumulation, and the addition of IbNAC56a or IbNAC56b caused increased pigmentation. Furthermore, we confirmed the interaction of IbMYB340 with IbbHLH2 and IbNAC56a or IbNAC56b via yeast two-hybrid and firefly luciferase complementation assays; these proteins could form a MYB340-bHLH2-NAC56a or MYB340-bHLH2-NAC56b transcriptional complex to regulate anthocyanin biosynthesis by binding to the IbANS promoter rather than the IbUFGT promoter. Furthermore, it was found by a transient expression system in tobacco leaves that IbMYB44 could decrease anthocyanin accumulation. Moreover, the interaction of IbMYB44 with IbMYB340 and IbNAC56a or IbNAC56b was verified. This result suggested that IbMYB44 acts as a repressor of anthocyanin in sweet potato.Conclusions: The repressor IbMYB44 affected anthocyanin biosynthesis by competitively inhibiting the IbMYB340-IbbHLH2-IbNAC56a or IbMYB340-IbbHLH2-IbNAC56b regulatory complex formation. Overall, the present study proposed a novel regulatory network whereby several vital TFs play key roles in regulating anthocyanin biosynthesis, and it provides strong insight into the potential mechanism underlying anthocyanin biosynthesis in sweet potato tuberous roots with purple color.

scholarly journals The Content of Phenolic Acids and Flavonols in the Leaves of Nine Varieties of Sweet Potatoes (Ipomoea batatas L.) Depending on Their Development, Grown in Central Europe

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3473 ◽  
Author(s):  
Barbara Krochmal-Marczak ◽  
Tomasz Cebulak ◽  
Ireneusz Kapusta ◽  
Jan Oszmiański ◽  
Joanna Kaszuba ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Phenolic Acids ◽  
Leaf Development ◽  
Growth Stage ◽  
Polyphenolic Compounds ◽  
Raw Material ◽  
Growth Stages ◽  
Potato Leaf ◽  
Antioxidant Power ◽  
Sweet Potato Leaves

The aim of the study was the qualitative and quantitative analysis of the bioactive components present in the leaves of 9 sweet potato cultivars grown in the moderate climate in Poland, which were harvested at different growth stages according to the BBCH (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) scale (14, 51, 89). It was found that sweet potato leaves contained 7 polyphenolic compounds, including 5 chlorogenic acids—neochlorogenic acid (5-CQA), chlorogenic acid (3-CQA), 4-cryptochlorogenic acid (4-CQA), 34-di-O-caffeoylqunic acid (3,4-CQA), 3,5-di-O-caffeoylqunic acid (3,5-CQA)—and 2 flavonoids, quercetin-3-O-galactoside (Q-3-GA) and quercetin-3-O-glucoside (Q-3-GL). Their content depended on the genotype of the examined cultivars and on the stage of leaf development. The mean content of the identified polyphenolic compounds in the examined cultivars ranged from 148.2 to 14.038.6 mg/100 g−1 DM for the leaves harvested at growth stage 14 according to the BBCH scale. In the case of leaves harvested at BBCH stage 51, the concentration of polyphenolic compounds ranged from 144.76 to 5026.8 mg/100 g−1 DM and at BBCH stage 89 from 4078.1 to 11.183.5 mg/100 g−1 DM. The leaves of the Carmen Rubin cultivar collected at stage 14 contained the highest amount of polyphenolic compounds, while Okinava leaves had the highest amount of these compounds at stage 51. The highest content of polyphenolic compounds in leaves at BBCH growth stage 89 was found in the Radiosa variety. The highest concentration levels were found for 3-CQA at all stages of leaf development. Significant correlations between polyphenol content and antioxidant activity measured by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing/antioxidant power (FRAP) were found. The results of this experiment revealed that the growth stages and genetic properties of cultivars have a very significant influence on the content of phenolic acids and flavonols in sweet potato leaves. The results are innovative and can have a practical application, as the knowledge of the content of the substances under study makes it possible to determine the optimal management practice of sweet potato leaf harvest in order to obtain more top-quality raw material.

scholarly journals Potential of Pantoea dispersa as an effective biocontrol agent for black rot in sweet potato

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lingmin Jiang ◽  
Jae Chul Jeong ◽  
Jung-Sook Lee ◽  
Jeong Mee Park ◽  
Jung-Wook Yang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Vascular Tissue ◽  
Potato Cultivar ◽  
Biocontrol Agents ◽  
Blue Staining ◽  
Black Rot ◽  
Promising Alternative ◽  
Post Harvest ◽  
Potato Plants ◽  
Tuberous Roots

Abstract Biocontrol offers a promising alternative to synthetic fungicides for the control of a variety of pre- and post-harvest diseases of crops. Black rot, which is caused by the pathogenic fungus Ceratocytis fimbriata, is the most destructive post-harvest disease of sweet potato, but little is currently known about potential biocontrol agents for this fungus. Here, we isolated several microorganisms from the tuberous roots and shoots of field-grown sweet potato plants, and analyzed their ribosomal RNA gene sequences. The microorganisms belonging to the genus Pantoea made up a major portion of the microbes residing within the sweet potato plants, and fluorescence microscopy showed these microbes colonized the intercellular spaces of the vascular tissue in the sweet potato stems. Four P. dispersa strains strongly inhibited C. fimbriata mycelium growth and spore germination, and altered the morphology of the fungal hyphae. The detection of dead C. fimbriata cells using Evans blue staining suggested that these P. dispersa strains have fungicidal rather than fungistatic activity. Furthermore, P. dispersa strains significantly inhibited C. fimbriata growth on the leaves and tuberous roots of a susceptible sweet potato cultivar (“Yulmi”). These findings suggest that P. dispersa strains could inhibit black rot in sweet potato plants, highlighting their potential as biocontrol agents.

Characterization of bacterial endophytes of sweet potato plants

2009 ◽  
Vol 322 (1-2) ◽  
pp. 197-207 ◽  
Author(s):  
Zareen Khan ◽  
Sharon L. Doty
Keyword(s):  
Sweet Potato ◽  
Bacterial Endophytes ◽  
Potato Plants

scholarly journals Relationship between bud number in seed branches and yield aspects of sweet potato

2021 ◽  
Vol 39 (4) ◽  
pp. 451-457
Author(s):  
Amanda C Perrud ◽  
Lorrayne G Bavaresco ◽  
André R Zeist ◽  
Murilo HS Leal ◽  
André D Silva Júnior ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Block Design ◽  
Ground Level ◽  
Growth And Yield ◽  
Root Number ◽  
Consumer Market ◽  
Market Values ◽  
Tuberous Roots ◽  
Randomized Complete Block Design ◽  
Below Ground

ABSTRACT Planting sweet potato branches with the appropriate bud number and disposition, below and above ground, can favor vegetative growth and yield that better fit the marketable standards. This study aimed to explore the influence of the number of buds and their distribution ratio, above and below ground level, on the agronomic and marketable components of sweet potato tuberous roots. The experiment was carried out in a randomized complete block design with three replications. The treatments were arranged in a factorial scheme (3 x 5), with 2, 4, and 8 above-ground buds combined with 2, 4, 6, 8, and 10 below-ground buds. Branches from the UZBD 06 accession (Canadense standard) were used. Vegetative, productive, and marketable traits of roots were evaluated. The use of branches with a greater number of buds above and below ground increased shoot dry biomass. Planting seed branches with 8 buds above and 8 buds below ground provided a greater number and production of marketable roots. The use of 10 buried buds increased root number and yield in the 150-450 g marketable classes, which the consumer market values the most.

Response of Sweet Potato Storability with Different Pre-storage Treatments at Ambient Temperature

2021 ◽  
Vol 19 (1) ◽  
pp. 10-17
Author(s):  
M.S Afolabi ◽  
W.A Lamidi ◽  
M.O Akoroda
Keyword(s):  
Weight Loss ◽  
Sweet Potato ◽  
Alkaline Solution ◽  
Climatic Conditions ◽  
Ambient Condition ◽  
Chromolaena Odorata ◽  
Storage Performance ◽  
Tuberous Roots ◽  
Storage Treatment ◽  
Pre Treatment

The storage of sweet potato tuberous roots in tropical countries is a major challenge to farmers and retailers due to unfavourable climatic conditions. In this study, the storage performance of sweet potato roots in an ambient condition was investigated over a 12-week duration. The three different pre-storage treatments used were ash, alkaline solution (1.5 mol dm-3) and Siam weed (Chromolaena odorata) extract – to evaluate their effects on weight loss, shrinkage, weevil damage and sprouting. The control comprised tubers with no pre-treatment. It was observed that weight loss increased linearly from the inception of storage while shrinkage became apparent only after two (2) weeks. The percentage shrinkage of the tuber pre-treated with Siam weed increased at decreasing rate from 8 WAP. Treatment of tuber with Siam weed extract provided significantly higher protection against weevil damage (p > 0.05). After this, sprouting was initiated. By the 12th week, the Siam weed (Chromolaena odorata) extract treatment yielded better results. The Siam weed (Chromolaena odorata) treatment also recorded the lowest weight loss of 28% and lowest shrinkage of 2.8%. It also sustained less weevil damage at 33.5% compared with the other treatments. Sprouting was higher in the tuber without pre-storage treatment. The alkaline solution and ash treatments had varied degree of performance for all the indicators. Keywords: Sweet potato storability, Ambient condition, Pre-storage treatment, Chromolaena odorata

Histochemical observations and gene expression changes related to internal browning in tuberous roots of sweet potato (Ipomea batatas)

Plant Science ◽  
2018 ◽  
Vol 274 ◽  
pp. 476-484 ◽  
Author(s):  
Nobuyuki Fukuoka ◽  
Masahiro Miyata ◽  
Tatsuro Hamada ◽  
Eishin Takeshita
Keyword(s):  
Gene Expression ◽  
Sweet Potato ◽  
Tuberous Roots ◽  
Ipomea Batatas ◽  
Internal Browning

Effect of Shoot Removal on Shoot and Root Yields of Sweet Potato

1985 ◽  
Vol 21 (2) ◽  
pp. 183-186 ◽  
Author(s):  
M. T. Dahniya ◽  
S. K. Hahn ◽  
C. O. Oputa
Keyword(s):  
Sweet Potato ◽  
Shoot Tips ◽  
Root Yield ◽  
Varietal Differences ◽  
Root Crop ◽  
Green Vegetable ◽  
Tuberous Roots ◽  
Shoot Removal

SUMMARYExperiments on harvesting sweet potato as a green vegetable and as a root crop are described. Whole shoots yielded 62% more than shoot tips. Similar total shoot yields were harvested whether tip removal was at two, three or four week intervals. Root yield was decreased by 31 to 48% by removing shoot tips, while removing whole shoots led to root yield decreases of 48 to 62%. Harvesting shoots at two week intervals gave 72% reduction in root yield, compared with 50% with four week intervals. There were fewer and smaller tubers as the frequency of shoot harvests increased. There were varietal differences in response to shoot removal For reasonable yields of both shoot tips and tuberous roots harvesting shoot tips at four week intervals is recommended.

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