Amaranthuscontrol in cotton can be difficult with the loss of glyphosate efficacy, especially in conservation-tillage cropping systems. Research was conduction from 2006 to 2008 at EV Smith Research Center, Shorter, AL, to determine the level of glyphosate-susceptibleAmaranthuscontrol provided by four initial tillage and herbicide treatments, including 1) moldboard plowing followed by a single-pass disking and field cultivation plus pendimethalin at 1.2 kg ai ha−1preplant incorporation (PPI), 2) two-pass disking followed by field cultivation plus pendimethalin at 1.2 kg ha−1PPI, 3) no tillage including an application of pendimethalin at 1.2 kg ha−1PRE, or 4) no tillage without pendimethalin in 2006. No further tillage practices or pendimethalin applications were utilized after study initiation. Initial tillage operations, including inversion with disking or disking twice, resulted inAmaranthusdensity of ≤ 4 plants m−2and 47 to 82% control, whereas no-tillage treatments had ≥ 4 plants m−2and 14 to 62% control. Subsequent applications of PRE herbicides included fluometuron at 1.68 kg ai ha−1or prometryn at 1.12 kg ai ha−1and provided 53 to 98% and 55 to 93% control, respectively, and reducedAmaranthusdensity compared to no PRE herbicide to < 2 plants m−2, regardless of tillage treatment. A POST application of glyphosate at 1.0 kg ae ha−1improved control in conjunction with almost all treatments in each year. Results indicate that a one-time tillage operation followed by a return to reduced tillage may aid in the reduction ofAmaranthusdensity when used with PRE-applied herbicides; however, this system will likely not provide adequate control when high population densities of glyphosate-resistantAmaranthusare present, thus highlighting the need for a highly efficacious POST herbicide system.