Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way

2010 ◽  
Vol 55 (3) ◽  
pp. 185-194 ◽  
Author(s):  
David M. Dohan Ehrenfest ◽  
Pierre Doglioli ◽  
Giuseppe M. de Peppo ◽  
Marco Del Corso ◽  
Jean-Baptiste Charrier
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
In Vitro Proliferation ◽  
Platelet Rich Fibrin ◽  
Proliferation And Differentiation ◽  
Dose Dependent

Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell In Vitro by E-Cadherin-Fc as a New Smart Biological Materials

2012 ◽  
Vol 554-556 ◽  
pp. 1785-1788
Author(s):  
Nan Jiang ◽  
Jian Bin Xu ◽  
Jun Yang
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Bioactive Materials ◽  
Proliferation And Differentiation ◽  
Coated Surfaces ◽  
Proliferation In Vitro ◽  
E Cadherin

There are many research shows that the umbilical cord was a “store house” of stem cells. Human umbilical cord mesenchymal stem cell(HUMSC) was considered to be an idea seed cell for cell therapy and cell models construction used in drug development. But the regulation of HUMSC proliferation and differentiation in vitro is facing challenges. This research aimed to construct a biomimic microenvironment using bioactive materials including the fusion protein of E-cadherin-Fc and promote HUMSC proliferation. The adhersion and proliferation of HUMSC measured by MTT assay, were obviously enhanced on E-cadherin-Fc coated surfaces, comparing with these on the gelatin coated surfaces and conventional conditions. Meanwhile, the expression of CD105 assayed by flow cytometry, showing that the pluripotency of HUMSC cultured on the E-cadherin-Fc coated surfaces were higher than that in the tissue treated plate. From the above, the establishment of E-cadherin-Fc microenvionment is beneficial for HUMSC proliferation in vitro.

scholarly journals THE EFFECT OF PLATELET RICH PLASMA ON MESENCHYMAL STEM CELLs (MSCs) DIFFERENTIATION INTO CHONDROBLAST

2019 ◽  
Vol 6 (2) ◽  
pp. 80
Author(s):  
Dwikora Novembri Utomo ◽  
I Gde Adi Widiastana
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Growth Media ◽  
Mesenchymal Stem Cell Culture ◽  
The Third ◽  
Proliferation And Differentiation ◽  
Differentiation And Proliferation

The addition of platelet rich plasma to mesenchymal stem cell culture on growth  media and  chondrogenic  media  had  any effect  on stem cell’s proliferation and differentiation into chondroblast has not been determined. This research is to find  out  the  effect  of  platelet  rich  plasma  on  mesenchymal  stem  cell’s differentiation and proliferation into chondroblast on in vitro media. Randomized control group posttest only design. Blood was taken from the rabbit’s vein to be processed into platelet rich plasma (PRP). Mesenchymal Stem Cell (MSC) was harvested from the bone marrow of the rabbit to be cultured. The MSC’s culture were divided into three groups of modification. The first group was combination of MSC added with Complete Culture Medium (CCM) and Chondrogenic Diferentation Medium (CDM) without PRP as control group. The second group had the same combination as the first group with extra 5% PRP. The third group had the same combination as the first group with extra 10% PRP. The results were evaluated in the following 21 days. The group that received extra 5% PRP had significant increase of chondroblast count compared to the group without PRP addition (p=0,033). The same result also occured on the groups that received extra 10% PRP compared to the group without PRP addition (p=0,028). There were no significant diferences between both the second and the third groupchondroblast count (p=0,203). There was a significant effect of platelet rich plasma on mesenchymal stem cell’s diferentiation and proliferation into chondroblast on invitro media.

Bone mesenchymal stem cell-derived exosomes-loaded injectable hydrogel for minimally invasive treatment of spinal cord injury

Nanomedicine ◽  
2021 ◽  
Author(s):  
Jiyun Cheng ◽  
Zheng Chen ◽  
Can Liu ◽  
Mei Zhong ◽  
Shihuan Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Therapeutic Effects ◽  
Invasive Treatment ◽  
Repair Materials ◽  
Cord Injury ◽  
Proliferation And Differentiation

Aim: Bone mesenchymal stem cell-derived exosomes (Exos) have been shown to exert therapeutic effects in spinal cord injury (SCI). In this study, we aimed to apply bioengineering approaches to promote Exo retention and their sustained release for SCI repair. Materials & methods: 3D gelatin methacrylate hydrogel (GelMA) was used as a transplanted Exo delivery system (GelMA-Exos). The viability, proliferation, and differentiation of neural stem cells cultured on hydrogel were assessed. Further, GelMA-Exos was injected into the damaged lesions to assess its repair potential. Results: GelMA hydrogel enhanced the retention of Exos, which promoted the neuronal differentiation and extension in vitro. Furthermore, GelMA-Exos promoted neurogenesis and attenuated glial scars in the damaged lesions. Conclusion: The injectable Exo-loaded 3D hydrogel induced neurological functional recovery post SCI.

In Vitro Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Regenerating Template

2015 ◽  
Vol 05 (999) ◽  
pp. 1-1
Author(s):  
Abu Bakar Mohd Hilmi ◽  
Mohd Noor Norhayati ◽  
Ahmad Sukari Halim ◽  
Chin Keong Lim ◽  
Zulkifli Mustafa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Human Hair ◽  
Human Hair Follicle ◽  
In Vitro Proliferation ◽  
Proliferation And Differentiation ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Uptake and Distribution of Administered Bone Marrow Mesenchymal Stem Cell Extracellular Vesicles in Retina

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 730
Author(s):  
Biji Mathew ◽  
Leianne A. Torres ◽  
Lorea Gamboa Gamboa Acha ◽  
Sophie Tran ◽  
Alice Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Extracellular Vesicles ◽  
Time Course ◽  
Ganglion Cells ◽  
Ex Vivo ◽  
Dependent Manner ◽  
Paracrine Effects

Cell replacement therapy using mesenchymal (MSC) and other stem cells has been evaluated for diabetic retinopathy and glaucoma. This approach has significant limitations, including few cells integrated, aberrant growth, and surgical complications. Mesenchymal Stem Cell Exosomes/Extracellular Vesicles (MSC EVs), which include exosomes and microvesicles, are an emerging alternative, promoting immunomodulation, repair, and regeneration by mediating MSC’s paracrine effects. For the clinical translation of EV therapy, it is important to determine the cellular destination and time course of EV uptake in the retina following administration. Here, we tested the cellular fate of EVs using in vivo rat retinas, ex vivo retinal explant, and primary retinal cells. Intravitreally administered fluorescent EVs were rapidly cleared from the vitreous. Retinal ganglion cells (RGCs) had maximal EV fluorescence at 14 days post administration, and microglia at 7 days. Both in vivo and in the explant model, most EVs were no deeper than the inner nuclear layer. Retinal astrocytes, microglia, and mixed neurons in vitro endocytosed EVs in a dose-dependent manner. Thus, our results indicate that intravitreal EVs are suited for the treatment of retinal diseases affecting the inner retina. Modification of the EV surface should be considered for maintaining EVs in the vitreous for prolonged delivery.

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