scholarly journals THE EFFECT OF PLATELET RICH PLASMA ON MESENCHYMAL STEM CELLs (MSCs) DIFFERENTIATION INTO CHONDROBLAST

2019 ◽  
Vol 6 (2) ◽  
pp. 80
Author(s):  
Dwikora Novembri Utomo ◽  
I Gde Adi Widiastana
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Growth Media ◽  
Mesenchymal Stem Cell Culture ◽  
The Third ◽  
Proliferation And Differentiation ◽  
Differentiation And Proliferation

The addition of platelet rich plasma to mesenchymal stem cell culture on growth  media and  chondrogenic  media  had  any effect  on stem cell’s proliferation and differentiation into chondroblast has not been determined. This research is to find  out  the  effect  of  platelet  rich  plasma  on  mesenchymal  stem  cell’s differentiation and proliferation into chondroblast on in vitro media. Randomized control group posttest only design. Blood was taken from the rabbit’s vein to be processed into platelet rich plasma (PRP). Mesenchymal Stem Cell (MSC) was harvested from the bone marrow of the rabbit to be cultured. The MSC’s culture were divided into three groups of modification. The first group was combination of MSC added with Complete Culture Medium (CCM) and Chondrogenic Diferentation Medium (CDM) without PRP as control group. The second group had the same combination as the first group with extra 5% PRP. The third group had the same combination as the first group with extra 10% PRP. The results were evaluated in the following 21 days. The group that received extra 5% PRP had significant increase of chondroblast count compared to the group without PRP addition (p=0,033). The same result also occured on the groups that received extra 10% PRP compared to the group without PRP addition (p=0,028). There were no significant diferences between both the second and the third groupchondroblast count (p=0,203). There was a significant effect of platelet rich plasma on mesenchymal stem cell’s diferentiation and proliferation into chondroblast on invitro media.

Promoting the Expansion of Human Umbilical Cord Mesenchymal Stem Cell In Vitro by E-Cadherin-Fc as a New Smart Biological Materials

2012 ◽  
Vol 554-556 ◽  
pp. 1785-1788
Author(s):  
Nan Jiang ◽  
Jian Bin Xu ◽  
Jun Yang
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Bioactive Materials ◽  
Proliferation And Differentiation ◽  
Coated Surfaces ◽  
Proliferation In Vitro ◽  
E Cadherin

There are many research shows that the umbilical cord was a “store house” of stem cells. Human umbilical cord mesenchymal stem cell(HUMSC) was considered to be an idea seed cell for cell therapy and cell models construction used in drug development. But the regulation of HUMSC proliferation and differentiation in vitro is facing challenges. This research aimed to construct a biomimic microenvironment using bioactive materials including the fusion protein of E-cadherin-Fc and promote HUMSC proliferation. The adhersion and proliferation of HUMSC measured by MTT assay, were obviously enhanced on E-cadherin-Fc coated surfaces, comparing with these on the gelatin coated surfaces and conventional conditions. Meanwhile, the expression of CD105 assayed by flow cytometry, showing that the pluripotency of HUMSC cultured on the E-cadherin-Fc coated surfaces were higher than that in the tissue treated plate. From the above, the establishment of E-cadherin-Fc microenvionment is beneficial for HUMSC proliferation in vitro.

Bone mesenchymal stem cell-derived exosomes-loaded injectable hydrogel for minimally invasive treatment of spinal cord injury

Nanomedicine ◽  
2021 ◽  
Author(s):  
Jiyun Cheng ◽  
Zheng Chen ◽  
Can Liu ◽  
Mei Zhong ◽  
Shihuan Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Therapeutic Effects ◽  
Invasive Treatment ◽  
Repair Materials ◽  
Cord Injury ◽  
Proliferation And Differentiation

Aim: Bone mesenchymal stem cell-derived exosomes (Exos) have been shown to exert therapeutic effects in spinal cord injury (SCI). In this study, we aimed to apply bioengineering approaches to promote Exo retention and their sustained release for SCI repair. Materials & methods: 3D gelatin methacrylate hydrogel (GelMA) was used as a transplanted Exo delivery system (GelMA-Exos). The viability, proliferation, and differentiation of neural stem cells cultured on hydrogel were assessed. Further, GelMA-Exos was injected into the damaged lesions to assess its repair potential. Results: GelMA hydrogel enhanced the retention of Exos, which promoted the neuronal differentiation and extension in vitro. Furthermore, GelMA-Exos promoted neurogenesis and attenuated glial scars in the damaged lesions. Conclusion: The injectable Exo-loaded 3D hydrogel induced neurological functional recovery post SCI.

scholarly journals The Analysis of the Relationship between RELA Gene Expression and MMP-13 Gene Expression in Synoviocyte Cells after Mesenchymal Stem Cell Wharton Jelly

2019 ◽  
Vol 7 (4) ◽  
pp. 543-548 ◽  
Author(s):  
Vivi Sofia ◽  
Ellyza Nasrul ◽  
Menkher Manjas ◽  
Gusti Revilla
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cartilage Damage ◽  
Cartilage Degradation ◽  
Cell Group ◽  
Control Group ◽  
Group I ◽  
The Relationship

BACKGROUND: Therapy that can cure osteoarthritis with satisfactory results has not been found to date. In the pathogenesis of osteoarthritis, the genes involved in cartilage degradation include the RELA gene which plays an important role in modulating the occurrence of cartilage damage, which involves activation of pro-inflammatory cytokines. One of the cytokines involved in the cartilage degradation process is Matrix Metalloproteinase (MMP) -13 which is also modulated by NFĸβ. AIM: This study aims to look at the expression of the RELA gene and expression of the MMP-13 gene and analyse the relationship of RELA gene expression with MMP-13 gene expression after administration of Mesenchymal Stem Cell Wharton Jelly in synoviocytes in vitro. MATERIAL AND METHODS: This research is pure experimental research. The samples used derived from synovial tissue in osteoarthritis patients who underwent surgery for Total Knee Replacement (TKR). This study was divided into 6 treatment groups with 4 replications. Group I was the synoviocyte OA cell control group which was incubated 24 hours, group II was control of synoviocyte OA cell which was incubated 48 hours, group III was a group of Mesenchymal Stem Cell Wharton Jelly (MSC-WJ) which was incubated 24 hours, group IV was a Mesenchymal Stem Cell Wharton Jelly (MSC-WJ) cell group incubated 48 hours, group V was the co-culture group of synoviocyte-MSC-WJ cells incubated 24 hours and group VI was the co-culture of synoviocyte-MSC-WJ cells which were incubated 48 hours. Observation of MMP-13 gene expression and RELA gene in each group was carried out using qPCR. RESULT: The results showed that the analysis of the relationship between RELA gene expression and MMP-13 gene expression in osteoarthritis synoviocytes cells after Mesenchymal Stem Cell Wharton Jelly as big as (r = 0.662). CONCLUSION: The conclusion of this study is there was a strong correlation between RELA gene expression and MMP-13 gene expression in osteoarthritis synoviocytes after Mesenchymal Stem Cell Wharton Jelly (r = 0.662).

scholarly journals Platelet-Rich Plasma Promotes Migration, Proliferation, and the Gene Expression of Scleraxis and Vascular Endothelial Growth Factor in Paratenon-Derived Cells In Vitro

2018 ◽  
Vol 11 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Sosuke Imai ◽  
Ken Kumagai ◽  
Yasuteru Yamaguchi ◽  
Kazuma Miyatake ◽  
Tomoyuki Saito
Keyword(s):  
Gene Expression ◽  
Platelet Rich Plasma ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Fold Increase ◽  
Control Group ◽  
Proliferation And Differentiation ◽  
Fetal Bovine ◽  
Endothelial Growth Factor

Background: Platelet-rich plasma (PRP) is a treatment option for tendon injury because of its effective tendon-healing properties. At the early stage of tendon repair, paratenon-derived cells (PDCs) are thought to play a more important role than tendon proper–derived cells (TDCs). However, there has been no study investigating the effects of PRP on PDCs. Hypothesis: PRP promotes the migration, proliferation, and differentiation of PDCs in vitro. Study Design: Controlled laboratory study. Methods: TDCs and PDCs were isolated from the tendon proper and paratenon of rat Achilles tendons and were cultured to the third passage. PRP was prepared from the rats using the double-spin method. Third-passage TDCs and PDCs were cultured in Dulbecco’s modified Eagle medium with 2% fetal bovine serum (control group) or 2% fetal bovine serum plus 5% PRP (PRP group), and cell migration, proliferation, and differentiation were evaluated. The relative mRNA expression levels of scleraxis (Scx), tenomodulin (Tnmd), collagen type I alpha 1 (Col1a1), collagen type III alpha 1 (Col3a1), and vascular endothelial growth factor A (VEGF) were examined by quantitative real-time reverse transcription polymerase chain reaction. Results: The cell migration rate was significantly higher in the PDCs of the PRP group than in the control group (1.4-fold increase; P = 0.02). Cell proliferation was significantly higher in the PDCs of the PRP group (2.2-fold increase; P < 0.01). In the PDCs, the gene expression levels of Scx, Col1a1, and VEGF were significantly increased by PRP (Scx: 2.0-fold increase, P = 0.01; Col1a1: 5.3-fold increase, P = 0.01; VEGF: 7.8-fold increase, P = 0.01), but the gene expression level of Tnmd, a factor for tendon maturation, was significantly reduced by PRP (0.11-fold decrease; P = 0.02). Conclusion: In vitro PRP promoted migration, proliferation, and tenogenic differentiation with the upregulation of Scx in PDCs. PRP also upregulated the expression of the angiogenic marker VEGF. Clinical Relevance: Our results suggest that PRP treatment in vitro may enhance the tendon-healing properties of PDCs at the initial stage of tendon repair.

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