Dual-excitation-wavelength fluorescence spectra and elastic scattering for differentiation of single airborne pollen and fungal particles

2011 ◽  
Vol 45 (8) ◽  
pp. 1555-1563 ◽  
Author(s):  
Yong-Le Pan ◽  
Steven C. Hill ◽  
Ronald G. Pinnick ◽  
James M. House ◽  
Richard C. Flagan ◽  
...  
Keyword(s):  
Elastic Scattering ◽  
Fluorescence Spectra ◽  
Airborne Pollen ◽  
Excitation Wavelength ◽  
Fungal Particles

scholarly journals Remote Laser Induced Fluorescence of Soils and Rocks

Photonics ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 411
Author(s):  
Vasily N. Lednev ◽  
Alexey F. Bunkin ◽  
Sergey M. Pershin ◽  
Mikhail Ya. Grishin ◽  
Diana G. Artemova ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Spectra ◽  
Space Exploration ◽  
Laser Induced Fluorescence ◽  
Excitation Wavelength ◽  
Extraterrestrial Life ◽  
Future Space ◽  
Lidar Sensing ◽  
First Time ◽  
Different Soils

The laser induced fluorescence spectroscopy was systematically utilized for remote sensing of different soils and rocks for the first time, to the best of our knowledge. Laser induced fluorescence spectroscopy measurements were carried out by the developed nanosecond LIDAR instrument with variable excitation wavelength (355, 532 and 1064 nm). LIDAR sensing of different Brazil soil samples have been carried out in order to construct a spectral database. The laser induced fluorescence spectra interpretation for different samples has been discussed in detail. The perspectives of LIDAR sensing of organic samples deposited at soils and rock have been discussed including future space exploration missions in the search for extraterrestrial life.

Study on Three-Dimensional Fluorescence Spectra of Oil Concentration in Water

2011 ◽  
Vol 322 ◽  
pp. 144-147
Author(s):  
Yu Tian Wang ◽  
Li Li Yang ◽  
Xin Qiong Lu
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Three Dimensional ◽  
Measurement Model ◽  
Excitation Wavelength ◽  
Experimental Basis ◽  
Oil In Water ◽  
Oil Concentration ◽  
Fluorescence Spectrometer

According to the Lambert-Beer laws, the fluorescence spectra of oil in water were investigated by measuring excitation-emission matrixes with FS920 fluorescence spectrometer. On the basis of the three-dimensional fluorescence spectra of oil in water, the excitation wavelength of 290nm and emission wavelength of 324nm were chosen for the quantitative analysis of oil in water. The results show that the concentration of oil in water is linearly proportional to the fluorescence intensity in the range of 1ppm-100ppm, and the measurement model of concentration is obtained with the linear correlation coefficient of 0.9993. The artificial samples has also been tested and the results are satisfactory. The recovery is in the range of 92%-106%. The research provides experimental basis for the quantitative analysis of multi-component oil in water.

scholarly journals Determination of adulterants in adulterant-fruit spirit blends using excitation-emission matrix fluorescence spectroscopy

2015 ◽  
Vol 8 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Michaela Jakubíková ◽  
Jana Sádecká ◽  
Pavel Májek
Keyword(s):  
Wavelength Range ◽  
Fluorescence Spectra ◽  
Reliable Method ◽  
Excitation Wavelength ◽  
Mean Square ◽  
Excitation Emission Matrix ◽  
Ethanol Level ◽  
Parallel Factor ◽  
Eem Fluorescence

Abstract This study introduces a reliable method to detect adulteration of spirit drinks. Excitation-emission matrix (EEM) fluorescence in combination with parallel factor analysis (PARAFAC) and partial least squares (PLS) regression was used to determine the content of water and ethanol in adulterated fruit spirit samples. EEM fluorescence spectra recorded in the emission wavelength range of 315–450 nm and in the excitation wavelength range of 240–305 nm were used for PARAFAC. The model created using PARAFAC-PLS was able to predict the water and ethanol level in adulterated apple spirit with the root mean square error of prediction (RMSEP) values of 1.9 % and 1.8 %, respectively. Regarding adulterated plum spirit, the RMSEP values of 0.7 % and 3.5 % were obtained for water and ethanol, respectively. The aim of this work was to determine whether EEM-PARAFAC can be used to distinguish between plum and apple spirit. Better results were obtained for apple spirit and the method is useful also for water-apple spirit blends.

Fluorescence spectroscopy for chromophore studies on bleached kraft pulps

Holzforschung ◽  
2007 ◽  
Vol 61 (5) ◽  
pp. 509-515 ◽  
Author(s):  
Sirje Liukko ◽  
Ville Tasapuro ◽  
Tiina Liitiä
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Spectra ◽  
Lignin Content ◽  
Excitation Wavelength ◽  
Kraft Pulps ◽  
Carbonyl Groups ◽  
Residual Lignin ◽  
Pulp Bleaching ◽  
Peroxide Bleaching ◽  
Brightness Reversion

Abstract Fluorescence techniques are highly sensitive and, since aromatic lignin is the most likely source of natural pulp fluorescence, they are suitable for detailed investigation of residual lignin in bleached pulps. Such investigations are important to our understanding of the bleachability and brightness reversion of pulps. In this study, the effect of bleaching on pulps was evaluated using fluorescence spectra of two softwood kraft pulps bleached in six elemental chlorine-free and totally chlorine-free sequences. Fluorescence spectra were recorded in each bleaching stage using four different instrument settings. In general, pulp fluorescence at excitation wavelengths of 270 and 350 nm increased as bleaching proceeded. Below a certain lignin content, however, pulp fluorescence measured at an excitation wavelength of 430 nm decreased with the residual lignin content. Carbonyl groups have a quenching effect on fluorescence, and the formation of carbonyl groups after oxygen and ozone delignification induced a decrease in pulp fluorescence. The increase in pulp fluorescence after peroxide bleaching stages was due to the removal of carbonyl groups. Reactions of chromophores during brightness reversion also had an effect on pulp fluorescence. These findings demonstrate that fluorescence spectroscopy provides an interesting tool for pulp bleaching studies.

scholarly journals THE EFFECTIVE OF DIFFERENT EXCITATION WAVELENGTHS ON THE IDENTIFICATION OF PLANT SPECIES BASED ON FLUORESCENCE LIDAR

2016 ◽  
Vol XLI-B1 ◽  
pp. 147-150
Author(s):  
Jian Yang ◽  
Wei Gong ◽  
Shuo Shi ◽  
Lin Du ◽  
Jia Sun ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Plant Species ◽  
Fluorescence Spectra ◽  
Principal Component ◽  
Laser Induced Fluorescence ◽  
Support Vector ◽  
Excitation Wavelength ◽  
Lidar System ◽  
Excitation Light

Laser-induced fluorescence (LIF) served as an active technology has been widely used in many field, and it is closely related to excitation wavelength (EW). The objective of this investigation is to discuss the performance of different EWs of LIF LiDAR in identifying plant species. In this study, the 355, 460 and 556 nm lasers were utilized to excite the leaf fluorescence and the fluorescence spectra were measured by using the LIF LiDAR system built in the laboratory. Subsequently, the principal component analysis (PCA) with the help of support vector machine (SVM) was utilized to analyse fluorescence spectra. For the three EWs, the overall identification rates of the six plant species were 80 %, 83.3 % and 90 %. Experimental results demonstrated that 556 nm excitation light source is superior to 355 and 460 nm for the classification of the plant species for the same genus in this study. Thus, an appropriate excitation wavelength should be considered when the LIF LiDAR was utilized in the field of remote sensing based on the LIF technology.

scholarly journals The Preparation of Green Fluorescence-Emissioned Carbon Dots/Poly(N-Isopropylacrylamide) Temperature-Sensitive Hydrogels and Research on Their Properties

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1171 ◽  
Author(s):  
Dan Zhao ◽  
Wenting Ma ◽  
Rong Wang ◽  
Xinzhou Yang ◽  
Jun Li ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fluorescence Spectra ◽  
Cell Imaging ◽  
Fluorescence Probes ◽  
Excitation Wavelength ◽  
Temperature Sensitive ◽  
Green Fluorescence ◽  
Microscope Imaging ◽  
One Step ◽  
Good Biocompatibility

Fluorescence/temperature-sensitive hydrogels, thanks to their properties in fluorescence and temperature sensitivity, have shown a promising outlook in the fields of drug delivery, cell imaging, etc., and thus become the focus of present research. This paper reports the preparation of green-fluorescence/temperature-sensitive hydrogels through one-step radical polymerization with green fluorescence-emissioned carbon dots as fluorescence probes and N-isopropylacrylamide as a monomer. UV-vis spectra, fluorescence spectra, and fluorescence microscope imaging have been used to characterize the prepared hydrogel, and to study their optical and temperature-sensitive properties. It was discovered that the emission of prepared hydrogel is excitation wavelength-dependent, pH responding, and excellent temperature-sensitive, as well as having good biocompatibility. The prepared hydrogel can also be applied as fluorescence ink in the fields of anti-counterfeit identification and appraisal.

Determination of Gentamicin Residual in Duck Meat Using Fluorescence Analysis Method

2014 ◽  
Vol 1033-1034 ◽  
pp. 638-642
Author(s):  
Xiao Wang ◽  
Jiang Xu ◽  
Mu Hua Liu ◽  
Jin Hui Zhao ◽  
Qian Hong
Keyword(s):  
Correlation Coefficient ◽  
Fluorescence Spectra ◽  
Limit Of Detection ◽  
Fluorescence Analysis ◽  
Excitation Wavelength ◽  
Analysis Method ◽  
Standard Samples ◽  
Meat Extract ◽  
Coefficient Of Regression

Gentamicin is a kind of aminoglycoside antibiotics and widely used in the prevention and treatment of the duck diseases. A prediction model was established for the rapid detection of Gentamicin residue in duck meat using fluorescence analysis method according to the strong fluorescent characteristic of the generated derivative for Gentamicin and o-phthaldialdehyde (OPA) in the presence of emulsifier OP-10 and mercaptoethanol.The fluorescence spectra of the duck meat containing Gentamicin were analyzed, the optimum excitation wavelength of the material was 340 nm and the optimum emission wavelength was 442 nm. Fluorescence intensity and the concentration of the standard samples presented the good linear relationship, the linear correlation coefficient was 0.9963 and the limit of detection was 0.47 μg/mL in the dynamic rang of 0.5 ~6.5μg/mL. The correlation coefficient of regression equation was 0.9968 for the samples of duck meat extract. The experimental results showed that the fluorescence analysis method had a good performance and accuracy in detecting the Gentamicin residue in duck meat.

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