In vitro protective effects of resveratrol against oxidative damage in human erythrocytes

Strawberry fruits are highly appreciated by consumers worldwide due to their bright red color, typical aroma, and juicy texture. While the biological activity of the complete fruit has been widely studied, the potential beneficial effects of the achenes (commonly named seeds) remain unknown. In addition, when raw fruit and achenes are consumed, the digestion process could alter the release and absorption of their phytochemical compounds, compromising their bioactivity. In the present work, we evaluated the protective effects against oxidative damage of nondigested and digested extracts from strawberry fruit and achenes in human hepatocellular carcinoma (HepG2) cells. For that purpose, cells were treated with different concentration of the extracts prior to incubation with the stressor agent, AAPH (2,2′-azobis(2-amidinopropane) dihydrochloride). Subsequently, intracellular accumulation of reactive oxygen species (ROS) and the percentage of live, dead, and apoptotic cells were determined. Our results demonstrated that all the evaluated fractions were able to counteract the AAPH-induced damage, suggesting that the achenes also present biological activity. The positive effects of both the raw fruit and achenes were maintained after the in vitro digestion process.

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Assessing the phenolic profile, antioxidant, antidiabetic and protective effects against oxidative damage in human erythrocytes of peaches from Fundão

Journal of Functional Foods ◽

10.1016/j.jff.2018.02.018 ◽

2018 ◽

Vol 43 ◽

pp. 224-233 ◽

Cited By ~ 7

Author(s):

Catarina Bento ◽

Ana C. Gonçalves ◽

Branca Silva ◽

Luís R. Silva

Keyword(s):

Oxidative Damage ◽

Human Erythrocytes ◽

Protective Effects ◽

Phenolic Profile

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A.265 In vitro flumazenil inhibits the bupivacaine protective effects on human erythrocytes haemolysis

British Journal of Anaesthesia ◽

10.1016/s0007-0912(18)31120-6 ◽

1996 ◽

Vol 76 ◽

pp. 84

Author(s):

F. Lenfant ◽

F. Volot ◽

M. Freysz ◽

L. Rochette

Keyword(s):

Human Erythrocytes ◽

Protective Effects

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The protective effects of Achillea L. species native in Turkey against H2O2-induced oxidative damage in human erythrocytes and leucocytes

Journal of Ethnopharmacology ◽

10.1016/j.jep.2005.06.018 ◽

2005 ◽

Vol 102 (2) ◽

pp. 221-227 ◽

Cited By ~ 54

Author(s):

Sibel Konyalioglu ◽

Canan Karamenderes

Keyword(s):

Oxidative Damage ◽

Human Erythrocytes ◽

Protective Effects

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Pyridoxine Decreases Oxidative Stress on Human Erythrocyte Membrane Protein in vitro

The Open Biochemistry Journal ◽

10.2174/1874091x01913010037 ◽

2019 ◽

Vol 13 (1) ◽

pp. 37-44 ◽

Cited By ~ 1

Author(s):

Margarita Velásquez ◽

Darío Méndez ◽

Carlos Moneriz

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Membrane Protein ◽

Oxidative Damage ◽

Erythrocyte Membrane ◽

Cumene Hydroperoxide ◽

Protein Carbonylation ◽

Human Erythrocytes ◽

Red Cell Membrane

Background: Pyridoxine has reduction and prevention against the levels of reactive oxygen species in in vitro studies. However, the biochemical mechanism that explains this behavior has not yet been fully clarified. Objective: To evaluate the effect of pyridoxine against oxidative damage on the membrane of human erythrocytes. Methods: Cumene hydroperoxide was used to induce oxidative stress in protein and lipid. Human erythrocytes were incubated with pyridoxine and cumene hydroperoxide, either alone or together for 8 h. Oxidative damage was determined by measuring lipid peroxidation and membrane protein carbonylation. Results: The results indicate that the malondialdehyde concentration decreased with increasing concentration of pyridoxine. The membrane protein content also decreased with increasing concentration of vitamin B6, which was confirmed by the decreased signal intensity in the western blot when compared to control without pyridoxine. Results demonstrate that pyridoxine can significantly decrease lipid peroxidation and protein carbonylation in red cell membrane exposed to high concentrations of oxidant agent. Conclusion: Pyridoxine showed a protective effect against the oxidative stress in human erythrocytes in vitro, inhibiting the carbonylation and the oxidative damage of erythrocyte membrane proteins. To date, such an effect has not yet been reported in terms of protein oxidation.

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After In Vitro Digestion, Jackfruit Flake Affords Protection against Acrylamide-Induced Oxidative Damage

Molecules ◽

10.3390/molecules24183322 ◽

2019 ◽

Vol 24 (18) ◽

pp. 3322 ◽

Cited By ~ 1

Author(s):

Daofeng Qu ◽

Chu Liu ◽

Mengxue Jiang ◽

Lifang Feng ◽

Yuewen Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

High Performance ◽

In Vitro Digestion ◽

Protective Effects ◽

Physical Damage ◽

Before And After ◽

High Content Analysis ◽

High Level

Some studies have demonstrated that acrylamide (AA) was correlated with oxidative stress, resulting in physical damage. The jackfruit flake was an immature pulp that contained a high level of antioxidant activity. This study aimed to assess the defensive efficacy of jackfruit flake in AA-induced oxidative stress before and after simulated gastrointestinal digestion. Our results indicate that the total polyphenol content of Jackfruit flake digest (Digestive products of jackfruit flake after gastrointestinal, JFG) was diminished; however, JFG had raised the relative antioxidant capacity compared to Jackfruit flake extract (JFE). Additionally, the results of High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) implied that a proportion of compounds were degraded/converted into other unknown and/or undetected metabolites. Further, by high content analysis (HCA) techniques, JFG markedly reduced cytotoxicity and excessive production of reactive oxygen species (ROS) in cells, thereby alleviating mitochondrial disorders. In this study, it may be converted active compounds after digestion that had preferable protective effects against AA-induced oxidative damage.

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