scholarly journals Synergistic reduction of toluylene blue induced by acetaldehyde and menadione in yeast cell suspension: Application to determination of yeast cell activity

2017 ◽  
Vol 9 ◽  
pp. 335-340 ◽  
Author(s):  
Shiro Yamashoji
Keyword(s):  
Yeast Cell ◽  
Cell Suspension ◽  
Cell Activity

scholarly journals Establishment and Fractionation of Metastatic Axillary Lymph Node Cell Suspension for Determination of Protein Expression Levels of Nuclear cFOS and Cytosolic TGFβ1 from Breast Cancer Patients

2021 ◽  
Author(s):  
Vesna Ivanović ◽  
Nasta Dedović-Tanić ◽  
Zorka Milovanović ◽  
Bratislav Stojiljković ◽  
Miroslav Demajo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Protein Expression ◽  
Cell Suspension ◽  
Axillary Lymph Node ◽  
Transforming Growth Factor ◽  
Axillary Lymph ◽  
Expression Levels ◽  
Nodal Cell

Abstract Background. Metastatic Axillary Lymph Node (mALN) status is currently the most important prognostic factor in the management of primary breast cancer (BC). Thus, development of specimens which enable identification of new mALN markers, involved in the progression of the disease, are of considerable interest. The specific aim of this work was to describe the method of establishment of Metastatic Axillary Nodal Cell Suspension and its fractionation, termed Fractionated Nodal Cell Suspension (FNCS), into nuclear and cytosolic extracts to enable determination of protein expression levels of nuclear cFOS and cytosolic Transforming Growth Factor β1 (TGFβ1) in BC patients. Results. To standardize the procedure, HeLa cells were successfully fractionated into nuclear/cytosolic extracts with confirmed presence of nuclear cFOS and cytosolic TGFβ1 proteins. Subsequently, the ALN Cell Suspension specimens were obtained and further fractionated from a pilot sample of six ALN tissue pairs, mALN versus autologous normal ALN (nALN), dissected from invasive BC patients. The mALN results revealed statistically significant overexpression in nuclear cFOS and cytosolic TGFβ1 protein levels, which was proportional to the respective values of mALN diameter of tumor deposits. Conclusions. Detailed protocol for establishment and fractionation of mALN cell suspension specimens, termed FNCS, into nuclear and cytosolic extracts is here described for the first time. This approach might be a convenient ex vivo model for simultaneous analysis of protein, RNA and DNA biomarkers from nuclear/cytosolic extracts of the same mALN tissue sample. It might have potential to enable, in the age of genomics and personalized medicine, an identification of novel mALN biomarkers and thus improve the screening, diagnosis and prognosis of invasive BC.

A reliable method for spectrophotometric determination of glycine betaine in cell suspension and other systems

2016 ◽  
Vol 498 ◽  
pp. 47-52 ◽  
Author(s):  
Ma Guadalupe Valadez-Bustos ◽  
Gerardo Armando Aguado-Santacruz ◽  
Axel Tiessen-Favier ◽  
Alejandrina Robledo-Paz ◽  
Abel Muñoz-Orozco ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Spectrophotometric Determination ◽  
Glycine Betaine ◽  
Reliable Method

scholarly journals Demonstration of lactase activity in culture medium of melon cells

2011 ◽  
Vol 31 (No. 4) ◽  
pp. 132-135
Author(s):  
J. Stano ◽  
K. Mičieta ◽  
E. Tokhtaeva ◽  
M. Valšíková ◽  
M. Koreňová ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Culture Medium ◽  
Agar Plate ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Lactase Activity ◽  
Seedling Roots ◽  
Intracellular Activities

Lactase activity was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple, rapid and reproducible procedure for identification of extracellular lactase is described using callus cultures of seedlings from the tested plant, hairy roots of 2.5 days old seedlings of watermelon germinating on agar plates as well as cell suspension cultures derived from callus cultures. For the determination of intracellular activities of lactase, 6-bromo-2-naphthyl-β-D-galactopyranoside and p-nitrophenyl-β-D-galactopyranoside were used as synthetic substrates. The extracellular lactase activity was determined by evaluating the day-zone in agar medium. The enzyme from watermelon callus cultures and seedling roots, cultivated on agar plates supplemented with 6-bromo-2-naphthyl-2-bromo-β-D-galactopyranoside, hydrolyzed this substrate releasing 6-bromo-naphthyl. By simultaneous coupling with hexazonium p-rosaniline or Fast Blue BB the corresponding azo dye was formed. The parallel extracellular and intracellular activities were determined in cell suspension cultures derived from callus cultures. The results show a 43.8% intracellular and 54.2% extracellular distribution of lactase activity. The described agar plate method enables a rapid, simple and specific detection of plant processes of extracellular lactase.  

scholarly journals Effect of Formulated Yeast in Suppressing the Liberation of Botrytis cinerea Conidia

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1265-1270 ◽  
Author(s):  
Darryl W. M. Cook
Keyword(s):  
Biological Control ◽  
Yeast Cell ◽  
Cell Suspension ◽  
Botrytis Cinerea ◽  
Fine Powder ◽  
Actinidia Deliciosa ◽  
Management Tool ◽  
Dry Powder ◽  
Epidemic Development ◽  
Leaf Disks

Eight yeast isolates that bound directly to Botrytis cinerea germlings were assessed for the ability to suppress spore liberation of conidia from B. cinerea. After the yeast cell suspension from each isolate was mixed with cellulose and dried, the product was milled into a fine powder. This yeast-cellulose formulation was applied as a dry powder to sporulating B. cinerea colonies on kiwifruit (Actinidia deliciosa) leaf disks, where the particles from the formulation attached to conidiophores and conidia. Some of these formulations significantly suppressed the liberation of conidia from treated colonies. Suppression of conidial liberation could provide another management tool for the biological control of sporulating B. cinerea with applications during late epidemic development. Using α-cellulose prepared with Candida pulcherrima in the conditions imposed in the present study, there was an approximately 50% reduction in the number of conidia released with the treatment of the B. cinerea lesions. The suppression of disease through a reduction in the population of liberated conidia is discussed.

scholarly journals Identification and quantitative determination of pinoresinol in Taxus ×media Rehder needles, cell suspension and shoot cultures

2015 ◽  
Vol 84 (1) ◽  
pp. 125-132 ◽  
Author(s):  
Paulina Mistrzak ◽  
Hanna Celejewska-Marciniak ◽  
Wojciech J. Szypuła ◽  
Olga Olszowska ◽  
Anna K. Kiss
Keyword(s):  
Cell Culture ◽  
Suspension Culture ◽  
Quantitative Determination ◽  
Cell Suspension ◽  
Dry Weight ◽  
Shoot Cultures ◽  
Taxus Media ◽  
In Vitro Shoot Cultures

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of <em>Taxus</em> ×<em>media</em>. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of <em>T.</em> ×<em>media</em>. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

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