Effects of specific pathogen-free porcine platelet-rich plasma activated by the novel activator ectoine on cell proliferation and biological function

Although the cases of endometrial carcinoma (EC) is gradually increasing across the world, its etiology and pathogenesis remain unknown. The present study is the first to define the role and biological function of circRNA hsa_circ_0075960 in the development and progression of EC. We first determined that hsa_circ_0075960 is aberrantly expressed in EC cells. Then, we uncovered that the downregulation of hsa_circ_0075960 suppressed cell proliferation and promoted cell apoptosis of EC cells, suggesting that hsa_circ_0075960 could inhibit the progression of EC in vitro. In addition, we identified that miR-361-3p was the direct target of hsa_circ_0075960. Further analysis revealed that hsa_circ_0075960 affected the development of EC via sponging miR-361-3p. Interestingly, we verified that the level of SH2B1 was controlled by the downregulation of hsa_circ_0075960 and that the negative effect caused by hsa_circ_0075960 could be reversed via miR-361-3p inhibition. Our cumulative results revealed that the novel tumor regulator hsa_circ_0075960 functioned as a sponge for miR-361-3p/SH2B1 in EC cells and regulated the progression of EC through the modulation of miR-361-3p.

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Feline Panleukopenia: II. The Relationship of Intestinal Mucosal Cell Proliferation Rates to Viral Infection and Development of Lesions

Veterinary Pathology ◽

10.1177/030098587701400209 ◽

1977 ◽

Vol 14 (2) ◽

pp. 173-181 ◽

Cited By ~ 14

Author(s):

J. H. Carlson ◽

F. W. Scott

Keyword(s):

Cell Proliferation ◽

Unit Length ◽

Mucosal Cell ◽

Proximal Jejunum ◽

Specific Pathogen Free ◽

Mucosal Cells ◽

Specific Pathogen ◽

Infected Cells ◽

Relationship Of ◽

The Relationship

Proliferation rates of small intestinal mucosal cells of noninfected germfree and specific pathogen-free kittens were compared to the incidence of infected cells and microscopic lesions in kittens experimentally infected with panleukopenia virus. Mucosal crypt length, cells per crypt, mitotic index and villous length were greater in specific pathogen-free kittens than in germfree kittens. Crypt cells per unit length and villous length per crypt length ratio were greater in germfree kittens. The cryptal cell proliferation rate of specific pathogen-free kittens was 2.24 times that of germfree kittens. Mucosal crypt length, cell per crypt and villous length were greater in the proximal jejunum than in the midjejunum of kittens within groups. Cell proliferation rates per crypt did not differ between areas of the intestine in kittens within groups. There were more virus-infected cells and lesions in specific pathogen-free kittens than in germfree kittens. The incidence of virus-infected cells and lesions was greater in the proximal jejunum and decreased along the small intestine.

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Industrial-scale processing of activated platelet-rich plasma from specific pathogen-free pigs and its effect on promoting human hair follicle dermal papilla cell cultivation

Journal of the Taiwan Institute of Chemical Engineers ◽

10.1016/j.jtice.2016.12.016 ◽

2017 ◽

Vol 71 ◽

pp. 28-37 ◽

Cited By ~ 3

Author(s):

Yung-Kai Lin ◽

Fendi Sugiri ◽

Hsu Ma ◽

Yen-Hao Chiu ◽

Chao-Ling Yao

Keyword(s):

Hair Follicle ◽

Human Hair ◽

Platelet Rich Plasma ◽

Dermal Papilla ◽

Industrial Scale ◽

Cell Cultivation ◽

Specific Pathogen Free ◽

Human Hair Follicle ◽

Dermal Papilla Cell ◽

Specific Pathogen

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In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium

Journal of the Taiwan Institute of Chemical Engineers ◽

10.1016/j.jtice.2017.06.006 ◽

2017 ◽

Vol 78 ◽

pp. 65-74 ◽

Cited By ~ 4

Author(s):

Yung-Kai Lin ◽

Ruchi Sharma ◽

Hsu Ma ◽

Wen-Shyan Chen ◽

Chao-Ling Yao

Keyword(s):

Corneal Endothelium ◽

Platelet Rich Plasma ◽

Specific Pathogen Free ◽

Specific Pathogen

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Laboratory Animal Research Centre (Larc) “A Specific Pathogen Free Rodent Facility” - Covid – 19 Pandemic Response Plan

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0285 ◽

2020 ◽

Author(s):

Muralitharan Shanmugakonar ◽

Vijay Kanth Govindharajan ◽

Kavitha Varadharajan ◽

Hamda Al-Naemi

Keyword(s):

Laboratory Animal ◽

Animal Research ◽

Research Centre ◽

Functional Requirement ◽

Specific Pathogen Free ◽

Specific Pathogen ◽

Response Plan ◽

Pandemic Response ◽

Operational Plan

Laboratory Animal Research Centre (LARC) has developed an early emergency operational plan for COVID-19 pandemic situation. Biosafety and biosecurity measures were planned and implemented ahead of time to check the functional requirement to prevent the infection. Identified necessary support for IT, transport, procurement, finance, admin and research to make the operations remotely and successfully.

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Magnesium-dependent Phosphatase (MDP) 1 is a Potential Suppressor of Gastric Cancer

Current Cancer Drug Targets ◽

10.2174/1568009619666190620112546 ◽

2019 ◽

Vol 19 (10) ◽

pp. 817-827

Author(s):

Jianbo Zhu ◽

Lijuan Deng ◽

Baozhen Chen ◽

Wenqing Huang ◽

Xiandong Lin ◽

...

Keyword(s):

Gastric Cancer ◽

Dna Methylation ◽

Cell Proliferation ◽

Protein C ◽

Biological Function ◽

Prognostic Markers ◽

Biological Effects ◽

Signal Transducer ◽

Gastric Cancer Cells ◽

Transcription 3

Background:Recurrence is the leading cause of treatment failure and death in patients with gastric cancer (GC). However, the mechanism underlying GC recurrence remains unclear, and prognostic markers are still lacking.Methods:We analyzed DNA methylation profiles in gastric cancer cases with shorter survival (<1 year) or longer survival (> 3 years), and identified candidate genes associated with GC recurrence. Then, the biological effects of these genes on gastric cancer were studied.Results:A novel gene, magnesium-dependent phosphatase 1 (mdp1), was identified as a candidate gene whose DNA methylation was higher in GC samples from patients with shorter survival and lower in patients with longer survival. MDP1 protein was highly expressed in GC tissues with longer survival time, and also had a tendency to be expressed in highly differentiated GC samples. Forced expression of MDP1 in GC cell line BGC-823 inhibited cell proliferation, whereas the knockdown of MDP1 protein promoted cell growth. Overexpression of MDP1 in BGC-823 cells also enhanced cell senescence and apoptosis. Cytoplasmic kinase protein c-Jun N-terminal kinase (JNK) and signal transducer and activator of transcription 3 (Stat3) were found to mediate the biological function of MDP1.Conclusion:These results suggest that MDP1 protein suppresses the survival of gastric cancer cells and loss of MDP expression may benefit the recurrence of gastric cancer.

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Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

Scientific Reports ◽

10.1038/s41598-021-90679-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Victor M. Petrone-Garcia ◽

Raquel Lopez-Arellano ◽

Gabriela Rodríguez Patiño ◽

Miriam Aide Castillo Rodríguez ◽

Daniel Hernandez-Patlan ◽

...

Keyword(s):

Pilot Study ◽

Broiler Chickens ◽

Solid Phase ◽

Oxidation Products ◽

Specific Pathogen Free ◽

Post Challenge ◽

Eimeria Maxima ◽

Lipid Oxidation Products ◽

Challenge Control ◽

Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

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