Combinatorial screening of cell proliferation on poly(l-lactic acid)/poly(d,l-lactic acid) blends

Biomaterials ◽  
2005 ◽  
Vol 26 (34) ◽  
pp. 6906-6915 ◽  
Author(s):  
Carl G. Simon ◽  
Naomi Eidelman ◽  
Scott B. Kennedy ◽  
Amit Sehgal ◽  
Chetan A. Khatri ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lactic Acid ◽  
Combinatorial Screening

Structure–property relation of porous poly (l-lactic acid) scaffolds fabricated using organic solvent mixtures and controlled cooling rates and its bio-compatibility with human adipose stem cells

2018 ◽  
Vol 33 (4) ◽  
pp. 397-415 ◽  
Author(s):  
Harish Chinnasami ◽  
Jeff Gimble ◽  
Ram V Devireddy
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Phase Separation ◽  
Mechanical Strength ◽  
Separation Method ◽  
Thermally Induced Phase Separation ◽  
Cooling Rates ◽  
Thermally Induced ◽  
Pore Sizes

Thermally induced phase separation method was used to make porous three-dimensional poly (l-lactic acid) scaffolds. The effect of imposed thermal profile during freezing of the poly (l-lactic acid) in dioxane solution on the scaffold was characterized by their micro-structure, porosity (%), pore sizes’ distribution, and mechanical strength. The porosity (%) decreased considerably with increasing concentrations of poly (l-lactic acid) in the solution, while a decreasing trend was observed with increasing cooling rates. The mechanical strength increases with increase in poly (l-lactic acid) concentration and also with increase in the cooling rate for both types of solvents. Therefore, mechanical strength was increased by higher cooling rates while the porosity (%) remained relatively consistent. Scaffolds made using higher concentrations of poly (l-lactic acid; 7% and 10% w/v) in solvent showed better mechanical strength which improved relatively with increasing cooling rates (1°C–40°C/min). This phenomenon of enhanced structural integrity with increasing cooling rates was more prominent in scaffolds made from higher initial poly (l-lactic acid) concentrations. Human adipose–derived stem cells were cultured on these scaffold (7% and 10% w/v) prepared by thermally induced phase separation at all cooling rates to measure the cell proliferation efficiency as a function of their micro-structural properties. Mean pore sizes played a crucial role in cell proliferation than percent porosity since all scaffolds were >88% porous. The viability percent of human adipose tissue–derived adult stem cells increased consistently with longer periods of culture. Thus, poly (l-lactic acid) scaffolds prepared by thermally controlled thermally induced phase separation method could be a prime candidate for making ex vivo tissue-engineered grafts for surgical implantation.

scholarly journals Influence of Poly-L-Lactic Acid Scaffold's Pore Size on the Proliferation and Differentiation of Dental Pulp Stem Cells

2015 ◽  
Vol 26 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Cristian Muniz Conde ◽  
Flávio Fernando Demarco ◽  
Luciano Casagrande ◽  
José Carlos Alcazar ◽  
Jacques Eduardo Nör ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Pore Size ◽  
Dental Pulp ◽  
Control Cell ◽  
Dental Pulp Stem Cells ◽  
Rt Pcr ◽  
Proliferation And Differentiation ◽  
Salt Crystals

The aim of this study was to evaluate the influence of the poly-L-lactic acid (PLLA)-based scaffold's pore size on the proliferation and differentiation of dental pulp stem cells (DPSCs). The scaffolds were prepared in pulp chambers of 1-mm-thick tooth slices from third molars using salt crystals (150-250 µm or 251-450 µm) as porogen. DPSC (1x105 cells) were seeded in the scaffolds with different pore sizes, and cultured in 24-well plates. The cell proliferation was evaluated using the WST-1 assay after 3-21 days. Furthermore, RT-PCR was used to assess the differentiation of the DPSCs into odontoblasts, using markers of odontoblastic differentiation (DSPP, DSP-1 and MEPE). RNA from human odontoblasts was used as control. Cell proliferation rate was similar in both scaffolds except at the 14th day period, in which the cells seeded in the scaffolds with larger pores showed higher proliferation (p<0.05). After 21 days DPSCs seeded in both evaluated scaffolds were able of expressing odontoblastic markers DMP-1, DSPP and MEPE. In summary, both scaffolds tested in this study allowed the proliferation and differentiation of DPSCs into odontoblast-like cells.

scholarly journals Osteogenic Differentiation of Pre-Conditioned Bone Marrow Mesenchymal Stem Cells with Nisin on the Modified Poly-L-Lactic-Acid Nanofibers

2021 ◽  
Author(s):  
Fariba Sadraei ◽  
Marzieh Ghollasi ◽  
Fatemeh Khakpai ◽  
Raheleh Halabian
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Lactic Acid ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Real Time Pcr ◽  
Alizarin Red ◽  
Marrow Mesenchymal Stem Cells

Abstract Background: Human bone marrow-derived mesenchymal stem (MSCs) cells are undifferentiated cells with the self-renewing ability and multi-lineage differentiation beneficial for regenerative medicine. Nano scaffolds are novel materials employed in bone repair and regeneration. Nisin is a prebiotic that can increase stem cells’ life span and proliferation. This study attempted to provide a proper strategy for bone marrow mesenchymal stem cells differentiation into the Osteocytes on a Poly‐L‐lactic‐acid scaffold (PLLA) after pretreating with probiotic Nisin. Methods: MSC osteogenic differentiation was evaluated by measuring Calcium, Alkaline phosphatase, and quantitative tests such as Real-Time PCR, Acridine Orange, Alizarin Red, Von Kossa, and others. Results: The result of the MTT test showed that the optimal dose of Nisin probiotic for the MSCs’ preconditioning was 200 IU/mL on the 1st, 3rd, and 5th days of culture. Real-time PCR data indicated that the expression rate of ALP, Osteonectin, Osteocalcin, and Collagen I have increased in the presence of Nisin, while the RUNX-2 gene expression has decreased. Furthermore, the results of Alizarin Red and Von Kossa tests, as well as Scanning electron microscopy (SEM), revealed that the cell proliferation in the preconditioned samples with Nisin increased significantly. Conclusions: The study concluded that the cell proliferation and differentiation increased in samples pretreated with Nisin on the PLLA Nano scaffolds.

scholarly journals 1,2,3,4,6-Penta-O-galloylglucose within Galla Chinensis Inhibits Human LDH-A and Attenuates Cell Proliferation in MDA-MB-231 Breast Cancer Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Shihab Deiab ◽  
Elizabeth Mazzio ◽  
Suresh Eyunni ◽  
Oshlii McTier ◽  
Nelly Mateeva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Cancer Cells ◽  
High Throughput Screening ◽  
Breast Cancer Cells ◽  
Lactic Acid Production ◽  
Dry Weight ◽  
Docking Studies ◽  
Human Breast

A characteristic feature of aggressive malignancy is the overexpression of lactic acid dehydrogenase- (LDH-) A, concomitant to pericellular accumulation of lactate. In a recent high-throughput screening, we identifiedRhus chinensis(Mill.) gallnut (RCG) (also known as Galla Chinensis) extract as a potent (IC50< 1 µg/mL) inhibitor of human LDH-A (hLDH-A). In this study, through bioactivity guided fractionation of the crude extract, the data demonstrate that penta-1,2,3,4,6-O-galloyl-β-D-glucose (PGG) was a primary constituent responsible forhLDH-A inhibition, present at ~9.95 ± 0.34% dry weight. Theoretical molecular docking studies ofhLDH-A indicate that PGG acts through competitive binding at the NADH cofactor site, effects confirmed by functional enzyme studies where the IC50= 27.32 nM was reversed with increasing concentration of NADH. Moreover, we confirm protein expression ofhLDH-A in MDA-231 human breast carcinoma cells and show that PGG was toxic (LC50= 94.18 µM), parallel to attenuated lactic acid production (IC50= 97.81 µM). In a 72-hour cell proliferation assay, PGG was found to be a potent cytostatic agent with ability to halt cell division (IC50= 1.2 µM) relative to paclitaxel (IC50< 100 nM). In summary, these findings demonstrate that PGG is a potenthLDH-A inhibitor with significant capacity to halt proliferation of human breast cancer cells.

Discovery of Novel Cell Proliferation-Enhancing Gene by Random siRNA Library Based Combinatorial Screening

2010 ◽  
Vol 13 (9) ◽  
pp. 798-806 ◽  
Author(s):  
Xiahui Xiong ◽  
Yabin Lu ◽  
Lishu Zhang ◽  
Bo Wang ◽  
Yingtao Zhao ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Combinatorial Screening ◽  
Sirna Library

Polypyrrole-coated poly(l-lactic acid-co-ε-caprolactone)/silk fibroin nanofibrous membranes promoting neural cell proliferation and differentiation with electrical stimulation

2016 ◽  
Vol 4 (41) ◽  
pp. 6670-6679 ◽  
Author(s):  
Binbin Sun ◽  
Tong Wu ◽  
Juan Wang ◽  
Dawei Li ◽  
Jing Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Electrical Stimulation ◽  
Lactic Acid ◽  
Silk Fibroin ◽  
Oxidative Polymerization ◽  
Neural Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Nanofiber Membranes

Conductive nanofiber membranes were developed by coating Ppy on PLCL/SF nanofibers via in situ oxidative polymerization.

scholarly journals P1.58 Lactic acid exerts anti-chlamydia trachomatisactivity on the epithelium by reducing host cell proliferation

2017 ◽  
Author(s):  
Vonetta Edwards ◽  
Elias Mccomb ◽  
Steven Smith ◽  
Patrik Bavoil ◽  
Jacques Ravel
Keyword(s):  
Cell Proliferation ◽  
Lactic Acid ◽  
Host Cell

Preparation of Poly(L-lactic Acid) Hybrid Membrane with Silicon-Ion-Releasing Ability

2007 ◽  
Vol 330-332 ◽  
pp. 1305-1308 ◽  
Author(s):  
Hirotaka Maeda ◽  
Emile Hideki Ishida ◽  
Toshihiro Kasuga
Keyword(s):  
Heat Treatment ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Body Fluid ◽  
Cellular Proliferation ◽  
Hybrid Membrane ◽  
Poly Lactic Acid ◽  
Calcium Carbonates ◽  
Before And After ◽  
Proliferation Ability

A novel poly(lactic acid) (PLA)/calcium carbonates hybrid membrane containing siloxane was prepared using aminopropyltriethoxysilane (APTES) for biodegradable bone guided regeneration. The PLLA in the membrane was an amorphous phase. By heating the membrane at 100 °C for 1 h, the PLLA in the membrane crystallized. Numerous pores of 0.5-1 ,m in diameter were newly formed at the surface. After soaking the membranes before and after heat-treatment in simulated body fluid, the amount of silicon species in SBF released from the membrane after heat-treatment was higher than that before heat-treatment. A test of osteoblast-like cellular proliferation on the membrane showed the membrane after heat-treatment has much higher cell-proliferation ability than that before heat-treatment.

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