Trends in 3D bioprinting for esophageal tissue repair and reconstruction

Chitosan is a unique natural resourced polysaccharide derived from chitin with special biocompatibility, biodegradability, and antimicrobial activity. During the past three decades, chitosan has gradually become an excellent candidate for various biomedical applications with prominent characteristics. Chitosan molecules can be chemically modified, adapting to all kinds of cells in the body, and endowed with specific biochemical and physiological functions. In this review, the intrinsic/extrinsic properties of chitosan molecules in skin, bone, cartilage, liver tissue repair, and organ three-dimensional (3D) bioprinting have been outlined. Several successful models for large scale-up vascularized and innervated organ 3D bioprinting have been demonstrated. Challenges and perspectives in future complex organ 3D bioprinting areas have been analyzed.

Download Full-text

Decellularized small intestine submucosa/polylactic-co-glycolic acid composite scaffold for potential application in hypopharyngeal and cervical esophageal tissue repair

Regenerative Biomaterials ◽

10.1093/rb/rbaa061 ◽

2021 ◽

Vol 8 (2) ◽

Author(s):

Shijie Qiu ◽

Lijin Liang ◽

Peng Zou ◽

Qi Chen

Keyword(s):

Small Intestine ◽

Tissue Repair ◽

Glycolic Acid ◽

Composite Scaffold ◽

Tensile Modulus ◽

In Vivo Studies ◽

Small Intestine Submucosa ◽

Cervical Esophageal Cancer ◽

Esophageal Tissue

Abstract There has been an increase in the incidence of hypopharyngeal and cervical esophageal cancer worldwide, and hence growing needs for hypopharyngeal and cervical esophageal tissue repair. This work produced a bi-layer composite scaffold with decellularized small intestine submucosa and polylactic-co-glycolic acid, which resembled the layered architectures of its intended tissues. The decellularized small intestine submucosa contained minimal residual DNA (52.5 ± 1.2 ng/mg) and the composite scaffold exhibited satisfactory mechanical properties (a tensile modulus of 21.1 ± 4.8 MPa, an ultimate tensile strength of 14.0 ± 2.9 MPa and a failure strain of 26.9 ± 5.1%). The interactions between cells and the respective layers of the scaffold were characterized by CCK-8 assays, immunostaining and Western blotting. Desirable cell proliferation and phenotypic behaviors were observed. These results have provided an important basis for the next-step in vivo studies of the scaffold, and bode well for its future clinical applications.

Download Full-text

3D Bioprinting of Bone Marrow Mesenchymal Stem Cell-Laden Silk Fibroin Double Network Scaffolds for Cartilage Tissue Repair

Bioconjugate Chemistry ◽

10.1021/acs.bioconjchem.0c00298 ◽

2020 ◽

Vol 31 (8) ◽

pp. 1938-1947 ◽

Cited By ~ 2

Author(s):

Tianyu Ni ◽

Min Liu ◽

Yajie Zhang ◽

Yi Cao ◽

Renjun Pei

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Silk Fibroin ◽

Tissue Repair ◽

Cartilage Tissue ◽

3D Bioprinting ◽

Double Network

Download Full-text

Stimuli-responsive supramolecules for bone tissue engineering

Biointerface Research in Applied Chemistry ◽

10.33263/briac102.122127 ◽

2020 ◽

Vol 10 (2) ◽

pp. 5122-5127

Keyword(s):

Tissue Engineering ◽

Ionic Strength ◽

Regenerative Medicine ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Repair ◽

3D Bioprinting ◽

Stimuli Responsive ◽

Hydrogel Scaffolds ◽

Physical Stimuli

In the recent scenario Stimuli responsive supramolecules are used for bone tissue engineering. Stimuli responsive Supramolecules are responding towards the desired stimuli. This has a property to change their dynamics and undergo impulsive and continual assembly or disassembly processes under specific conditions. These supramolecules respond towards chemical and physical stimuli which include: pH, temperature, light, ionic strength, magnetic and electric field sensitive. Stimuli responsive supramolecules are used to various preparations such as hydrogels, scaffolds, hydrogel scaffolds, 3D bioprinting, 4D bioprinting, nanogels and microgels used for the bone tissue repair and regenerative medicine. Manuscript deals with various approaches used to prepare stimuli responsive supramolecules for bone engineering applications.

Download Full-text

Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.746564 ◽

2021 ◽

Vol 9 ◽

Author(s):

Xue Han ◽

Shuai Chang ◽

Mingming Zhang ◽

Xiangbing Bian ◽

Chunlin Li ◽

...

Keyword(s):

Tissue Engineering ◽

Tissue Repair ◽

Cartilage Tissue Engineering ◽

Three Dimensional ◽

Synthetic Polymers ◽

Cartilage Tissue ◽

3D Bioprinting ◽

Natural Polymers ◽

Fabrication Condition ◽

Biological Performance

Bioprinting has gained immense attention and achieved the revolutionized progress for application in the multifunctional tissue regeneration. On account of the precise structural fabrication and mimicking complexity, hydrogel-based bio-inks are widely adopted for cartilage tissue engineering. Although more and more researchers have reported a number of literatures in this field, many challenges that should be addressed for the development of three-dimensional (3D) bioprinting constructs still exist. Herein, this review is mainly focused on the introduction of various natural polymers and synthetic polymers in hydrogel-based bioprinted scaffolds, which are systematically discussed via emphasizing on the fabrication condition, mechanical property, biocompatibility, biodegradability, and biological performance for cartilage tissue repair. Further, this review describes the opportunities and challenges of this 3D bioprinting technique to construct complex bio-inks with adjustable mechanical and biological integrity, and meanwhile, the current possible solutions are also conducted for providing some suggestive ideas on developing more advanced bioprinting products from the bench to the clinic.

Download Full-text

A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation

Regenerative Biomaterials ◽

10.1093/rb/rbab026 ◽

2021 ◽

Vol 8 (3) ◽

Author(s):

Hongqing Chen ◽

Fei Fei ◽

Xinda Li ◽

Zhenguo Nie ◽

Dezhi Zhou ◽

...

Keyword(s):

Soft Tissue ◽

Cell Viability ◽

Tissue Repair ◽

Three Dimensional ◽

Water Retention Capacity ◽

3D Bioprinting ◽

Soft Tissue Repair ◽

Retention Capacity

Abstract Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

Download Full-text