Ethyl acetate fraction of Pteris vittata L. alleviates 2‐acetylaminofluorene induced hepatic alterations in male Wistar rats

2017 ◽  
Vol 88 ◽  
pp. 1080-1089 ◽  
Author(s):  
Paramjeet Kaur ◽  
Manish Kumar ◽  
Amrit Pal Singh ◽  
Satwinderjeet Kaur
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethyl Acetate Fraction ◽  
Pteris Vittata ◽  
Male Wistar Rats ◽  
Pteris Vittata L

scholarly journals ANTIOBESITY OF FRACTIONS WATER EXTRACT OF TAMARIND (TAMARINDUS INDICA L.) IN MALE WISTAR RATS INDUCED HIGH CARBOHYDRATE DIET

2018 ◽  
Vol 11 (13) ◽  
pp. 200
Author(s):  
Nurul Hidayah ◽  
Ketut Adnyana I ◽  
Ketut Adnyana I ◽  
Neng Fisheri ◽  
Neng Fisheri ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Wistar Rats ◽  
Water Extract ◽  
Ethyl Acetate Fraction ◽  
High Carbohydrate Diet ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Male Wistar Rats ◽  
Weight Decrease

Objective: The prevalence of obesity increases each year globally. Multifactorial etiology of obesity requires therapy management including changing of diet and medicines. Some of obesity drugs have shown ineffectiveness and safety. The previous study showed that water extract of tamarind could reduce body weight (bw). This study aimed to test the activity fraction of water extract tamarind as antiobesity using high carbohydrate diet.Method: The preventive research of antiobesity had done by given water fraction and ethyl acetate fraction of water extract tamarind following with induced high carbohydrate diet during 6th weeks in male Wistar rats. The parameters had observed including consumption of food, body weight, weight of feces, volume of urine, total cholesterols, triglycerides, blood glucose, index of organs, and accumulation of body fat.Result: The ethyl acetate fraction at doses 4.5 mg/kg bw has shown significantly effect to decrease of total cholesterols level and decrease of triglycerides level at weeks 6 (p<0.05). All the tests of fraction have shown activity inhibition of increased body weight, decrease of appetite, total cholesterols, triglycerides, and blood glucose. Meanwhile, mechanism action of antiobesity as increase defecation, urination, and decrease index of organs and accumulation of body fat have not shown by all these test fractions.Conclusion: The ethyl acetate fraction at doses of 4.5 mg/kg bw can inhibit raising of body weight, decrease of total cholesterols, and triglycerides level greater than the other test groups, where increasing of these levels of blood biochemistry was closely related to the pathology of obesity.

scholarly journals DIURETIC ACTIVITY OF MATOA LEAVES EXTRACTS (Pometiapinnata) AND ITS INFLUENCE ON POTASSIUM AND SODIUM LEVELS

2017 ◽  
Vol 10 (14) ◽  
pp. 31
Author(s):  
Ika Purwidyaningrum ◽  
Elin Yulinah Sukandar ◽  
Irda Fidrianny
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Urine Volume ◽  
Ethyl Acetate Fraction ◽  
Control Group ◽  
Diuretic Activity ◽  
Observation Study ◽  
Aqueous Fraction ◽  
Male Wistar Rats ◽  
Sodium And Potassium

Objective: The purpose of this study was to determine diuretic activity of matoa leaves (Pometiapinnata) extracts and fraction and its influence on potassium and sodium levels.Methods: Matoa leaves were extracted by reflux method followed by evaporation using rotary evaporator. The subjects were male Wistar rats that were divided into 11 group furosemide (3.6 mg/kg bw), control group CMC 0.5%, matoa leaves extracts with doses of 50 mg/kg bw, 100 mg/kg bw, 150 mg/kg bw, matoa leaves aqueous fraction with dose of 10.94 mg/kg bw, 21.88 mg/kg bw, 32.82 mg/kg bw, matoa leaves ethyl acetate fraction with dose of 4.35 mg/kg bw, 8.71 mg/kg bw, 13.06 mg/kg bw. Rats were placed in metabolic cagesduring observation study. Urine volume was measured for 5 to 24 hours. Potassium and sodium levels in urine were determined by using Atomic Absorption Spectrophotometry.Results: The effective dose of matoa leaves extract and fractions for diuretic activity was matoaleaves ethyl acetate fraction 8.71 mg/kg bw which could increase the excretion of sodium and potassium in the urine of the male Wistar rats.Conclusion:Matoa leaves extract and fractions could increase the excretion of sodium and potassium in the urine of the male Wistar rats.  

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Anti-Diabetic Effects of the Ethyl-Acetate Fraction of Trichilia catigua in Streptozo-tocin-Induced Type 1 Diabetic Rats

2017 ◽  
Vol 42 (3) ◽  
pp. 1087-1097 ◽  
Author(s):  
Rodrigo Mello Gomes ◽  
Luis Fernando de Paulo ◽  
Cynthia Priscilla do Nascimento Bonato Panizzon ◽  
Camila Quaglio Neves ◽  
Bruna Colombo Cordeiro ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Kidney Tissue ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Male Wistar Rats ◽  
Food And Water Intake ◽  
Pancreas Morphology ◽  
Group D

Background/Aims: Trichilia catigua A. Juss., known as “catuaba” in Brazil, has been popularly used as a tonic for fatigue, impotence and memory deficits. Previously, our group demonstrated that the ethyl-acetate fraction (EAF) of T. catigua has antioxidant and anti-inflammatory effects. The present study evaluated the anti-diabetic activity of EAF in type 1 diabetic rats. Methods: Male Wistar rats were divided into four groups (N: non-diabetic group, D: type 1 diabetic group, NC: non-diabetic + EAF group and DC: type 1 diabetic + EAF group). The latter two groups were treated with 200 mg/kg EAF. Type 1 diabetes was induced by intravenous streptozotocin (STZ) injection (35 mg/kg). Starting two days after STZ injection, EAF was administered daily by gavage for 8 weeks. Results: EAF attenuated body mass loss and reduced food and water intake. EAF improved hyperglycaemia and other biochemical parameters, such as alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Furthermore, the number of pancreatic β-cells and the size of the islets had increased by β-cell proliferation in the DC group. EAF promoted reduction in kidney tissue damage in STZ-induced diabetic rats by reduction of renal fibrosis. Conclusion: The present study showed that EAF improves glucose homeostasis and endocrine pancreas morphology and inhibits the development of diabetic nephropathy in STZ-induced diabetic rats.

scholarly journals In vivo bioactivity-guided isolation of antifertility fraction of Waltheria indica Linn. root in male Wistar rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Basiru Afisu ◽  
Aremu Abdulfatah ◽  
Azeez Oyebisi Mistura ◽  
Soetan Kehinde Olugboyega ◽  
Olayemi Funsho Olakitike
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Soluble Fraction ◽  
Standard Procedure ◽  
Sperm Concentration ◽  
Ethanol Extract ◽  
Reproductive Hormones ◽  
Sperm Parameters ◽  
Male Wistar Rats

Abstract Background Waltheria indica is a multipurpose medicinal plant with abundance of phytochemical compounds. Antifertility effect of Waltheria indica Linn. root and leaves have been reported. However, the fraction responsible for this antifertility effect needs to be isolated for possible male contraceptive purpose. Therefore, this research was designed to isolate the antifertility fraction of Waltheria indica Linn. root (WILR) in an in vivo model using male Wistar rats. Crude ethanol extract of WILR was sequentially dissolved in hexane, dichloromethane, and ethyl acetate. Rats (n = 5) were administered with 200, 500, or 1000 μg/kg of hexane, dichloromethane, and ethyl acetate soluble extracts of WILR, while control received distilled water, daily for 15 days to determine the soluble extract with most antifertility effect. Thereafter, fractions were separated from dichloromethane soluble WILR extract by column and thin-layer chromatography. Rats (7 groups, n = 5) were administered with each of the fractions (DF1 to DF7; at 1000 μg/kg) to determine the fraction with the highest antifertility. Rats were thereafter sacrificed, and sperm parameters, reproductive hormones, testicular cholesterol, and protein were determined according to standard procedure. Histology of the testis was also done. Data were analyzed using ANOVA at p ≤ 0.05. Results Dichloromethane soluble fraction (500 μg/kg) significantly decreased sperm concentration (137.00 ± 9.85 to 107.00 ± 13.08 × 106 cells/mL), levels of testosterone (2.90 ± 0.65 to 1.50 ± 0.37 ng/mL), and FSH (0.08 ± 0.08 to 0.99 ± 0.08 IU/L). The dichloromethane soluble fraction also caused the loss of testicular interstitium and spermatogenic cells. DF5 significantly reduced sperm motility (92.00 ± 2.74 to 76.00 ± 5.48%) and LH (2.86 ± 0.52 to 1.47 ± 0.18 IU/L). DF5 also significantly increased levels of prolactin (1.22 ± 0.10 to 1.88 ± 0.48 ng/mL), testicular total protein (7.36 ± 0.35 to 8.54 ± 1.06 g/dL), and testicular cholesterol (34.17 ± 3.65 to 55.76 ± 6.08 mg/mL). Conclusion The results indicate that the DF5 is the bioactive fraction of WILR responsible for its antifertility effect. The possible antifertility mechanisms are through the reduction in sperm parameters, reproductive hormones, and histological changes in the testis.

scholarly journals Ethyl acetate fraction of Psorospermum febrifugum Spach aqueous extract did not exhibit acute or sub-chronic toxicity. Experimental study on Wistar rats

2019 ◽  
Vol 11 (4) ◽  
pp. 123
Author(s):  
F Agbogba ◽  
M Sènou ◽  
AP Tchogou ◽  
JE Lokonon ◽  
TI Sacramento ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Oral Toxicity ◽  
Function Tests

Psorospermum febrifugum Spach (Clusiaceae) was a tropical plant whose root bark was used to treat anemia. This work aimed to evaluate the safety of the ethyl acetate fraction of the aqueous extract of this bark. Methods: The ethyl acetate fraction of the extract was administered to Wistar rats in a single dose of 2000 mg / Kg body weight for acute oral toxicity test or daily doses of 200 mg / Kg of body weight during 28 days for sub-chronic oral toxicity test, as recommended by the OECD. At day 0, then at day 14 for the acute phase and day 28 for the sub-chronic phase, the rats were weighed and their blood collected for tests. The activity of transaminases AST and ALT were measured in the liver function tests, blood urea and creatinine were measured for renal function tests and blood leukocytes were counted for the immune balance. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. Results: In acute and sub-chronic oral toxicity tests, rat&rsquo;s weight, liver, kidney and immune balances as well as these organs histology were not affected, suggesting the safety of the extract fraction. Conclusion: The ethyl acetate fraction of the aqueous extract of the root bark of Psorospermum febrifugum did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids which have cytoprotective effects. The study of biological tolerance deserves to be continued by the chronic toxicity test and appropriate clinical trials.

scholarly journals ACTIVITY OF ETHYL ACETATE EXTRACT FROM Chrysophyllum cainito L. LEAVES IN DECREASING BLOOD SUGAR LEVEL IN MALE WISTAR RATS

2018 ◽  
Vol 3 (1) ◽  
pp. 31 ◽  
Author(s):  
Imam Malikul Hadi Arrijal ◽  
Burhan Ma'arif ◽  
Arief Suryadinata
Keyword(s):  
Ethyl Acetate ◽  
Blood Sugar ◽  
Blood Level ◽  
Blood Sugar Level ◽  
Wistar Rats ◽  
Ethyl Acetate Extract ◽  
Negative Control ◽  
Treatment Groups ◽  
Male Wistar Rats ◽  
Chrysophyllum Cainito

<p><em>The high morbidity or mortality due to diabetes mellitus becomes the world health problem. That fact encourages the study about antidiabetes drugs from natural material especially plants. This study aims to analyze the effect of </em><em>ethyl acetate extract from </em><em>Chrysophyllum cainito L. leaves </em><em>in</em><em> decreasing of blood sugar level of alloxan-induced male male wistar rats. This research is considered as experimental research with pre and post test randomized controlled group design. The experimental animals in this study were 25 male male wistar rats divided into 5 treatment groups, treatment with CMC-Na (negative control), metformin with the dose of 9 mg/200gBW (positive control), and leaves extract C.</em><em> </em><em>cainito dose 25; 50; 75 mg/kgBW. All treatment groups had significant decrease of blood sugar levels at 7, 10 and 14 day observations with p &lt;0.05. While in the day 3 had insignificant decrease of blood sugar level with p &gt;0.05. Every C.</em><em> </em><em>cainito leaves extract doses group have the ability to decrease sugar blood level better than negative control, but still below </em><em>positif control</em><em>. The optimum dose in decreasing sugar blood level in this study is 75 mg/kg</em><em> </em><em>BW.</em></p>

scholarly journals EFEKTIVITAS FRAKSI AKTIF EKSTRAK ETANOL LABU SIAM (Sechium edule (Jack) Sw) SEBAGAI ANTIHIPERTENSI PADA TIKUS HIPERTENSI YANG DIINDUKSI MONOSODIUM GLUTAMAT

2021 ◽  
Vol 18 (01) ◽  
pp. 1
Author(s):  
Yance Anas ◽  
Ika Nilam Cahyani ◽  
Ulum Firnanda Sukma
Keyword(s):  
Ethyl Acetate ◽  
Antihypertensive Effect ◽  
Monosodium Glutamate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antihypertensive Activity ◽  
Hypertensive Rats ◽  
Sechium Edule ◽  
Induced Hypertension ◽  
Male Wistar Rats

ABSTRACT Previous studies accomplished that the chayote ethanol extract (Sechium edule (Jack) Sw) had an antihypertensive effect. To get and identify the active compound, we fractionated the extract and evaluated its antihypertensive activity. This study aims to investigate the antihypertensive effect of the n-hexane fraction (HF-CEE) and ethyl acetate fraction of chayote ethanol extract (EAF-CEE) in monosodium glutamate (MSG)-induced hypertensive rats. We made the chayote extract using the maceration method by soaking the chayote simplicia in ethanol 70%. HF-CEE and EAF-CEE were obtained by stratified fractionation using n-hexane and ethyl acetate. An antihypertensive effect of the fraction measured on MSG-induced hypertension male Wistar rats after MSG 100 mg/kg BW/day (p.o) treatment for 14 days. The study concluded that HF-CEE and EAF-CEE had an antihypertensive effect in MSG-induced hypertensive rats. The EAF-CEE antihypertensive effect is higher than HF-CEE. Therefore, the FEAF-CEE active compounds need to be isolated, identified, developed, and their potential as an antihypertensive candidate. Keywords: Antihypertensive, chayote, ethyl-acetate fraction, Sechium edule, monosodium glutamate

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