Arquitectura y anatomía foliar de Chrysophyllum cainito L. y comparación con otras especies de la familia sapotaceae

Chrysophyllum cainito es un árbol frutal originario de Panamá, que a pesar de su importancia alimenticia y medicinal, existen pocos trabajos sobre su anatomía foliar. Se realizó una colecta de hojas en Cárdenas, Tabasco, México, y en el laboratorio se aplicaron las técnicas histológicas tradicionales para estudiar la arquitectura y anatomía foliar. En las muestras se identificó el patrón de venación y se determinó el grosor de la epidermis abaxial y adaxial, mesófilo, tipo y tamaño de los estomas, frecuencia estomática y se identificaron los tricomas, así como los tejidos de la nervadura central. La arquitectura foliar de esta especie es similar en algunas características a la de C. rufum y Manilkara spp. en cuanto a que son broquidódromas como la primera y que presentan venas intersecundarias y terciarias dispuestas al azar como las segundas. Se encontraron coincidencias en la forma rectangular de las células epidérmicas de este estudio con C. cainito nigeriano y C. rufum. Los estomas paracíticos son peculiares sólo en las hojas de C. cainito analizadas y en la especie tailandesa. La nervadura principal de C. cainito se considera bicolateral en un arco cerrado análogo con C. cainito tailandés. Así mismo, en este trabajo se identificaron coincidencias con las especies de la familia Sapotaceae a la que pertenece, como son la presencia de una capa epidérmica uniestratificada, hojas hipoestomáticas, cristales de oxalato de calcio, y tricomas tectores y ramificados (en “T”) ubicados en la superficie abaxial. Se aportaron nuevos atributos tales como el espesor de la epidermis, tamaño de los estomas, frecuencia estomática y detalles de la nervadura central.

Molecular docking analysis of CDK-1 inhibitors from Chrysophyllum cainito leaves

It is of interest to document the molecular docking analysis of Cyclin-dependent kinase 1 (CDK-1) inhibitors from Chrysophyllum cainito leaves towards the treatment of tumors using the known structure of PDB ID: 5HQ0. Data shows that molecules such as 8- (Dimethylamino)-7-(3-(4-ethylphenoxy)-2d, ethyl 6-oxo-5-propylheptanoate, 2,3-dihydro-3, 5-dihydroxy-6-methyl-4h-pyran-4-one, 1,2,3- benzenetriol and 1,4-benzenediol 2,5-bis (1,1-dimethylethyl) identified in methanolic extract of C. cainito have binding features with CDK1 for further consideration.

Anastrepha serpentina (sapodilla fruit fly).

A. serpentina is a pest of various cultivated species of Sapotaceae, especially caimito [Chrysophyllum cainito], sapodilla [Manilkara zapota], and sapote [Capparis angulata], and it occasionally attacks a variety of other hosts, including Citrus spp. It is a widespread species in the American tropics, occurring from Mexico to Argentina. It has been intercepted and trapped in the USA (Florida, California) and other countries outside its range indicating its potential for spread via infested fruits.

Pengaruh Gel Ekstrak Daun Kenitu (Chrysophyllum cainito L.) terhadap Reepitelisasi pada Penyembuhan Luka Bakar Mukosa Bukal Tikus Wistar

Burn therapy involves elimination of causative factors and symptomatic therapy. Kenitu leaves (Chrysophyllum cainito L.) are known to contain β-amylin acetate, Gallic acid, ursolic acid, quercetin, β-sitosterol, and other substances that have the potential to be antibacterial, antioxidant, and anti-inflammatory. The use of kenitu leaves (Chrysophyllum cainito L.) as an alternative to wound healing has low side effects and is easy to obtain, especially in Jember Regency. The aim of this study was to examine the effect of kenitu leaves (Chrysophyllum cainito L.) leaf extract gel on re-epithelialization in buccal mucosal burns of wistar rats. This type of research is experimental laboratories. This research used 32 samples which divided into four control groups and four treatment groups. Burns are made on the left buccal mucosa. In the control group the CMC placebo gel was given and the treatment group was given 2% leaf extract gel. Euthanasia was performed on days 4, 7, 10, and 13 then histological preparations were made. Measurement using software optilab viewer 2.2 and image raster 3.0. The average yield of epithelial thickness was lowest in the control group on day 4 and highest in the treatment group on day 13. The results of the One Way Anova statistical test showed a significant difference in epithelial thickness between the control group and the treatment group. This study proved that the 2% leaf extract can accelerate the process of reepithelialization in the healing of wistar mucosal mucosal burns.

Aqueous extract from Chrysophyllum cainito bark exhibits embryonic toxicity in Danio rerio and negligible acute toxicity in adult wistar rats

Chrysophyllum cainito has been used as a traditional medicine to treat a wide range of diseases, but the toxicity profile of this plant remains unknown. This study aimed to evaluate the acute toxicity of the aqueous extract of C. cainito (CE) bark based on OECD guidelines in two different in vivo experimental models: acute single-dose oral toxicity in adult Wistar rats and the zebrafish embryo acute toxicity test. All concentrations of CE (500-4000 mg/kg) tested during a 14-day period in both male and female rats showed no effect on behavior, body weight, organ weights, biochemical and hematological parameters. In contrast, CE significantly delayed zebrafish embryo hatching and decreased embryo survival rates in a dose-dependent manner. Hatched larvae were notably sensitive to CE-induced toxicity compared to unhatched fish embryos. Acridine orange staining showed that CE induced apoptosis in the yolk sac region that is responsible for supplying nutrients to support larval growth and development. According to OECD guidelines, CE was identified as GHS category 5, a substance with low to no acute toxicity. However, as embryotoxicity was observed in zebrafish, CE use during pregnancy should be exercised with caution until further examination of its safety.