Arsenic-induced uterine apoptotic damage is protected by ethyl acetate fraction of Camellia sinensis (green tea) via Bcl-2-BAX through NF-κB regulations in Wistar rats

2021 ◽  
Author(s):  
Arindam Dey ◽  
Hasina Perveen ◽  
Arjun L Khandare ◽  
Amrita Banerjee ◽  
Smarajit Maiti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Ethyl Acetate Fraction ◽  
Apoptotic Damage

scholarly journals Evaluation of antidiabetic and antioxidative efficacy of ethyl acetate fraction of methanolic extract of Camellia sinensis (green tea) leaves in streptozotocin induced diabetic albino rat

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Baisakhi Biswas ◽  
Barnali Das ◽  
Kishalay Jana ◽  
Debidas Ghosh
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rat ◽  
Hepatic Tissue ◽  
Tea Leaves ◽  
Green Tea Leaves

<p><strong>Objective:</strong> To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves in streptozotocin induced diabetic rat. <strong>Methods:</strong> Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic <em>Hexokinase-I</em>, pro-apoptotic <em>Bax</em> and anti-apoptotic <em>Bcl-2</em> gene expression patterns were noted by qRT-PCR technique. <strong>Results:</strong> After treatment with ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p &lt; 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p &lt; 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of <em>Hexokinase-I</em>, anti-apoptotic <em>Bcl-2</em> and high level of expression of pro-apoptotic <em>Bax</em> gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. <strong>Conclusion: </strong>From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of <em>C. sinensis</em> has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.</p>

Healing mechanisms of the hydroalcoholic extract and ethyl acetate fraction of green tea (Camellia sinensis (L.) Kuntze) on chronic gastric ulcers

2015 ◽  
Vol 389 (3) ◽  
pp. 259-268 ◽  
Author(s):  
Débora Gasparin Borato ◽  
Camila Toledo Scoparo ◽  
Daniele Maria-Ferreira ◽  
Luísa Mota da Silva ◽  
Lauro Mera de Souza ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethyl Acetate Fraction ◽  
Gastric Ulcers ◽  
Hydroalcoholic Extract

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Effect of green tea extract (Camellia sinensis) on the spermatic parameters of Wistar rats submitted or not to testicular heat shock

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Joane Isis Travassos Vieira ◽  
Taylane Alves da Silva ◽  
Williane Maria Pereira Barbosa ◽  
Gabriela Lima de Azevêdo ◽  
Lúcia Cristina Pereira Arruda ◽  
...  
Keyword(s):  
Heat Shock ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Wistar Rats ◽  
Green Tea Extract ◽  
Tea Extract

scholarly journals Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

2017 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Naniek Widyaningrum ◽  
Badie'ah Badie'ah ◽  
Sri Lestari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Staphylococcus Epidermidis ◽  
Economic Value ◽  
Diffusion Method ◽  
Tea Leaves ◽  
Green Tea Leaves

The dregs of green tea leaves have not been used until today, it is still considered as a waste that is not useful. It is known that the dregs of the green tea leaves contain Epigallocatechin-3-gallate (EGCG), which is quite high. EGCG has benefits as an antibacterial. The long-term goal of this research is the application of cosmetic preparation products from the dregs of green tea leaves that have effectiveness, stability and high economic value. The specific target of this research is to get the dose and concentration of the dregs of green tea leaves in a stable and effective way as anti-acne against Staphylococcus epidermidis (S. epidermidis). The search of the dregs of green tea leaves is by techniques of infundation of liquid fractions with ethyl acetate, identification of EGCG compound by using HPLC, antibiotics test against S. epidermidis was conducted by diffusion method. The results showed that the content of EGCG of the dregs of green tea leaves was 21.325% w/w, and the concentration of 1% of the dregs of green tea leaves can inhibit the growth of S. epidermidis well.

scholarly journals ANTIOBESITY OF FRACTIONS WATER EXTRACT OF TAMARIND (TAMARINDUS INDICA L.) IN MALE WISTAR RATS INDUCED HIGH CARBOHYDRATE DIET

2018 ◽  
Vol 11 (13) ◽  
pp. 200
Author(s):  
Nurul Hidayah ◽  
Ketut Adnyana I ◽  
Ketut Adnyana I ◽  
Neng Fisheri ◽  
Neng Fisheri ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Wistar Rats ◽  
Water Extract ◽  
Ethyl Acetate Fraction ◽  
High Carbohydrate Diet ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Male Wistar Rats ◽  
Weight Decrease

Objective: The prevalence of obesity increases each year globally. Multifactorial etiology of obesity requires therapy management including changing of diet and medicines. Some of obesity drugs have shown ineffectiveness and safety. The previous study showed that water extract of tamarind could reduce body weight (bw). This study aimed to test the activity fraction of water extract tamarind as antiobesity using high carbohydrate diet.Method: The preventive research of antiobesity had done by given water fraction and ethyl acetate fraction of water extract tamarind following with induced high carbohydrate diet during 6th weeks in male Wistar rats. The parameters had observed including consumption of food, body weight, weight of feces, volume of urine, total cholesterols, triglycerides, blood glucose, index of organs, and accumulation of body fat.Result: The ethyl acetate fraction at doses 4.5 mg/kg bw has shown significantly effect to decrease of total cholesterols level and decrease of triglycerides level at weeks 6 (p<0.05). All the tests of fraction have shown activity inhibition of increased body weight, decrease of appetite, total cholesterols, triglycerides, and blood glucose. Meanwhile, mechanism action of antiobesity as increase defecation, urination, and decrease index of organs and accumulation of body fat have not shown by all these test fractions.Conclusion: The ethyl acetate fraction at doses of 4.5 mg/kg bw can inhibit raising of body weight, decrease of total cholesterols, and triglycerides level greater than the other test groups, where increasing of these levels of blood biochemistry was closely related to the pathology of obesity.

scholarly journals Ethyl acetate fraction of Psorospermum febrifugum Spach aqueous extract did not exhibit acute or sub-chronic toxicity. Experimental study on Wistar rats

2019 ◽  
Vol 11 (4) ◽  
pp. 123
Author(s):  
F Agbogba ◽  
M Sènou ◽  
AP Tchogou ◽  
JE Lokonon ◽  
TI Sacramento ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Oral Toxicity ◽  
Function Tests

Psorospermum febrifugum Spach (Clusiaceae) was a tropical plant whose root bark was used to treat anemia. This work aimed to evaluate the safety of the ethyl acetate fraction of the aqueous extract of this bark. Methods: The ethyl acetate fraction of the extract was administered to Wistar rats in a single dose of 2000 mg / Kg body weight for acute oral toxicity test or daily doses of 200 mg / Kg of body weight during 28 days for sub-chronic oral toxicity test, as recommended by the OECD. At day 0, then at day 14 for the acute phase and day 28 for the sub-chronic phase, the rats were weighed and their blood collected for tests. The activity of transaminases AST and ALT were measured in the liver function tests, blood urea and creatinine were measured for renal function tests and blood leukocytes were counted for the immune balance. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. Results: In acute and sub-chronic oral toxicity tests, rat&rsquo;s weight, liver, kidney and immune balances as well as these organs histology were not affected, suggesting the safety of the extract fraction. Conclusion: The ethyl acetate fraction of the aqueous extract of the root bark of Psorospermum febrifugum did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids which have cytoprotective effects. The study of biological tolerance deserves to be continued by the chronic toxicity test and appropriate clinical trials.

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