Towards a reliable technology for antioxidant capacity and oxidative damage evaluation: Electrochemical (bio)sensors

There are close links among hyperglycaemia, oxidative stress and diabetic complications. Glutamine (GLN) is an amino acid with immunomodulatory properties. The present study investigated the effect of dietary GLN on oxidative stress-relative gene expressions and tissue oxidative damage in diabetes. There were one normal control (NC) and two diabetic groups in the present study. Diabetes was induced by an intraperitoneal injection of nicotinamide followed by streptozotocin (STZ). Rats in the NC group were fed a regular chow diet. In the two diabetic groups, one group (diabetes mellitus, DM) was fed a common semi-purified diet while the other group received a diet in which part of the casein was replaced by GLN (DM-GLN). GLN provided 25 % of total amino acid N. The experimental groups were fed the respective diets for 8 weeks, and then the rats were killed for further analysis. The results showed that blood thioredoxin-interacting protein (Txnip) mRNA expression in the diabetic groups was higher than that in the NC group. Compared with the DM group, the DM-GLN group had lower glutamine fructose-6-phosphate transaminase 1, a receptor of advanced glycation end products, and Txnip gene expressions in blood mononuclear cells. The total antioxidant capacity was lower and antioxidant enzyme activities were altered by the diabetic condition. GLN supplementation increased antioxidant capacity and normalised antioxidant enzyme activities. Also, the renal nitrotyrosine level and Txnip mRNA expression were lower when GLN was administered. These results suggest that dietary GLN supplementation decreases oxidative stress-related gene expression, increases the antioxidant potential and may consequently attenuate renal oxidative damage in rats with STZ-induced diabetes.

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Exogenous hesperidin and chlorogenic acid alleviate oxidative damage induced by arsenic toxicity in Zea mays by regulating the water status, antioxidant capacity, redox balance and fatty acid composition

Environmental Pollution ◽

10.1016/j.envpol.2021.118389 ◽

2021 ◽

pp. 118389

Author(s):

Busra Arikan ◽

Ceyda Ozfidan-Konakci ◽

Evren Yildiztugay ◽

Gokhan Zengin ◽

Fatma Nur Alp ◽

...

Keyword(s):

Zea Mays ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Chlorogenic Acid ◽

Acid Composition ◽

Water Status ◽

Redox Balance ◽

Arsenic Toxicity

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Effect of different metal ions on the oxidative damage and antioxidant capacity of hyaluronic acid

Archives of Biochemistry and Biophysics ◽

10.1016/s0003-9861(02)00661-6 ◽

2003 ◽

Vol 410 (1) ◽

pp. 76-82 ◽

Cited By ~ 35

Author(s):

György T. Balogh ◽

János Illés ◽

Zsuzsanna Székely ◽

Erika Forrai ◽

Anikó Gere

Keyword(s):

Hyaluronic Acid ◽

Oxidative Damage ◽

Metal Ions ◽

Antioxidant Capacity

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Vitamin E and protection of DNA, proteins and lipids from oxidative damage: evaluation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006

EFSA Journal ◽

10.2903/j.efsa.2016.4588 ◽

2016 ◽

Vol 14 (10) ◽

pp. e04588

Author(s):

◽

Dominique Turck ◽

Jean-Louis Bresson ◽

Barbara Burlingame ◽

Tara Dean ◽

...

Keyword(s):

Vitamin E ◽

Oxidative Damage ◽

Damage Evaluation ◽

Health Claim

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Discussion of “Effect of exercise-induced dehydration on circulatory markers of oxidative damage and antioxidant capacity”

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2018-0031 ◽

2018 ◽

Vol 43 (5) ◽

pp. 522-522

Author(s):

Abolfazl Zahedi ◽

Ali Amani-Beni

Keyword(s):

Oxidative Damage ◽

Antioxidant Capacity ◽

Exercise Induced

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Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity

Antioxidants ◽

10.3390/antiox9090794 ◽

2020 ◽

Vol 9 (9) ◽

pp. 794 ◽

Cited By ~ 1

Author(s):

Celeste Santos-Rosendo ◽

Fernando Bugatto ◽

Alvaro González-Domínguez ◽

Alfonso M. Lechuga-Sancho ◽

Rosa Maria Mateos ◽

...

Keyword(s):

Nitric Oxide ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Nitrosative Stress ◽

Antioxidant Response ◽

Placental Function ◽

Total Antioxidant ◽

Metabolically Healthy ◽

Inducible Nitric Oxide

Pregnancy-related disorders, including preeclampsia and gestational diabetes, are characterized by the presence of an adverse intrauterine milieu that may ultimately result in oxidative and nitrosative stress. This scenario may trigger uncontrolled production of reactive oxygen species (ROS) such as superoxide anion (O●−) and reactive nitrogen species (RNS) such as nitric oxide (NO), along with an inactivation of antioxidant systems, which are associated with the occurrence of relevant changes in placental function through recognized redox post-translational modifications in key proteins. The general objective of this study was to assess the impact of a maternal obesogenic enviroment on the regulation of the placental nitroso-redox balance at the end of pregnancy. We measured oxidative damage markers—thiobarbituric acid-reacting substances (TBARS) and carbonyl groups (C=O) levels; nitrosative stress markers—inducible nitric oxide synthase, nitrosothiol groups, and nitrotyrosine residues levels; and the antioxidant biomarkers—catalase and superoxide dismutase (SOD) activity and expression, and total antioxidant capacity (TAC), in full-term placental villous from both pre-pregnancy normal weight and obese women, and with absence of metabolic complications throughout gestation. The results showed a decrease in C=O and TBARS levels in obese pregnancies. Although total SOD and catalase concentrations were shown to be increased, both activities were significantly downregulated in obese pregnancies, along with total antioxidant capacity. Inducible nitric oxide sintase levels were increased in the obese group compared to the lean group, accompanied by an increase in nitrotyrosine residues levels and lower levels of nitrosothiol groups in proteins such as ERK1/2. These findings reveal a reduction in oxidative damage, accompanied by a decline in antioxidant response, and an increase via NO-mediated nitrative stress in placental tissue from metabolically healthy pregnancies with obesity. All this plausibly points to a placental adaptation of the affected antioxidant response towards a NO-induced alternative pathway, through changes in the ROS/RNS balance, in order to reduce oxidative damage and preserve placental function in pregnancy.

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Self-Reported Exposure to ETS (Environmental Tobacco Smoke), Urinary Cotinine, and Oxidative Stress Parameters in Pregnant Women—The Pilot Study

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16091656 ◽

2019 ◽

Vol 16 (9) ◽

pp. 1656 ◽

Cited By ~ 3

Author(s):

Lubica Argalasova ◽

Ingrid Zitnanova ◽

Diana Vondrova ◽

Monika Dvorakova ◽

Lucia Laubertova ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Antioxidant Capacity ◽

Pregnant Women ◽

Environmental Tobacco Smoke ◽

Tobacco Smoke ◽

Passive Smoking ◽

Oxidative Stress Parameters ◽

And Oxidative Stress ◽

Stress Parameters

Background: Exposure to ETS (environmental tobacco smoke) is one of the most toxic environmental exposures. Objective: To investigate the association of ETS with physiological, biochemical, and psychological indicators, as well as with urine antioxidant capacity (AC) and oxidative damage to lipids in a pilot sample of healthy pregnant women. Methods: Exposure to ETS was investigated via a validated questionnaire, and urine cotinine and the marker of oxidative damage to lipids via 8-isoprostane concentrations using an ELISA kit. Urine AC was determined by the spectrophotometric Trolox-equivalent antioxidant capacity (TEAC) method. From a sample of pregnant women (n = 319, average age 30.84 ± 5.09 years) in 80, the levels of cotinine and oxidative stress markers were analyzed. Results: Among the 80 pregnant women, 5% (7.4% confirmed by cotinine) reported being current smokers and 25% reported passive smoking in the household (18.8% confirmed by cotinine). The Kappa was 0.78 for smokers and 0.22 for ETS-exposed nonsmokers. Pregnant women in the ETS-exposed group had significantly reduced AC compared to both the nonsmoker (ETS−) and the smoker groups (p < 0.05). Nonsmokers had significantly lower levels of 8-isoprostane than smokers (p < 0.01) and ETS-exposed nonsmokers (p < 0.05). Correlations between urine levels of cotinine and AC were positive in ETS-exposed nonsmokers. Conclusion: A harmful association of active and passive smoking and oxidative stress parameters among pregnant women has been indicated.

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Chronic quercetin ingestion and exercise-induced oxidative damage and inflammation

Applied Physiology Nutrition and Metabolism ◽

10.1139/h07-177 ◽

2008 ◽

Vol 33 (2) ◽

pp. 254-262 ◽

Cited By ~ 49

Author(s):

Steven R. McAnulty ◽

Lisa S. McAnulty ◽

David C. Nieman ◽

John C. Quindry ◽

Peter A. Hosick ◽

...

Keyword(s):

Oxidative Damage ◽

Antioxidant Capacity ◽

Flavonoid Compound ◽

Trolox Equivalent Antioxidant Capacity ◽

C Reactive Protein ◽

Reactive Protein ◽

Reducing Ability ◽

Trolox Equivalent ◽

Exercise Induced

Quercetin is a flavonoid compound that has been demonstrated to be a potent antioxidant in vitro. The objective of this study was to evaluate if quercetin ingestion would increase plasma antioxidant measures and attenuate increases in exercise-induced oxidative damage. Forty athletes were recruited and randomized to quercetin or placebo. Subjects consumed 1000 mg quercetin or placebo each day for 6 weeks before and during 3 d of cycling at 57% work maximum for 3 h. Blood was collected before and immediately after exercise each day, and analyzed for F2-isoprostanes, nitrite, ferric-reducing ability of plasma, trolox equivalent antioxidant capacity, and C-reactive protein. Statistical analyses involved a 2 (treatment) × 6 (times) repeated measures analysis of variance to test main effects. F2-isoprostanes, nitrite, ferric-reducing ability of plasma, trolox equivalent antioxidant capacity, and C-reactive protein were significantly elevated as a result of exercise, but no group effects were found. Despite previous data demonstrating potent antioxidant actions of quercetin in vitro, this study indicates that this effect is absent in vivo and that chronic quercetin ingestion does not exert protection from exercise-induced oxidative stress and inflammation.

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