Synthesis and sensing integration: A novel enzymatic reaction modulated Nanoclusters Beacon (NCB) “Illumination” strategy for label-free biosensing and logic gate operation

2016 ◽  
Vol 86 ◽  
pp. 588-594 ◽  
Author(s):  
Lu Hong ◽  
Fu Zhou ◽  
Guangfeng Wang ◽  
Xiaojun Zhang
Keyword(s):  
Logic Gate ◽  
Enzymatic Reaction ◽  
Label Free ◽  
Gate Operation

scholarly journals Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Han Yan ◽  
Zhen Zhang ◽  
Ting Weng ◽  
Libo Zhu ◽  
Pang Zhang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Detection Method ◽  
Logic Gate ◽  
Detection Methods ◽  
Label Free ◽  
Recognition Method ◽  
Operation Pattern ◽  
Simple Detection ◽  
Dna Tetrahedron ◽  
Marker Molecule

Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.

Redox-induced target-dependent ratiometric fluorescence sensing strategy and logic gate operation for detection of α-glucosidase activity and its inhibitor

2021 ◽  
Author(s):  
Xucan Yuan ◽  
Yi Sun ◽  
Pengfei Zhao ◽  
Longshan Zhao ◽  
Zhili Xiong
Keyword(s):  
Redox Reaction ◽  
Logic Gate ◽  
Sensitive Detection ◽  
Ratiometric Fluorescence ◽  
Fluorescence Sensing ◽  
Gate Operation ◽  
Glucosidase Activity ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Ratiometric Fluorescence Sensing

A target-dependent ratiometric fluorescence sensing strategy was designed and fabricated based on redox reaction for highly sensitive detection of α-glucosidase (α-Glu) activity and its inhibitor. In this study, silicon quantum...

scholarly journals Establishing MALDI-TOF as Versatile Drug Discovery Readout to Dissect the PTP1B Enzymatic Reaction

2018 ◽  
Vol 23 (6) ◽  
pp. 561-573 ◽  
Author(s):  
Martin Winter ◽  
Tom Bretschneider ◽  
Carola Kleiner ◽  
Robert Ries ◽  
Jörg P. Hehn ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput Screening ◽  
Tyrosine Phosphatase ◽  
Enzymatic Reaction ◽  
Enzymatic Reactions ◽  
Label Free ◽  
Ionization Time ◽  
Maldi Tof ◽  
Ptp1b Inhibitors ◽  
Promising Perspective

Label-free, mass spectrometric (MS) detection is an emerging technology in the field of drug discovery. Unbiased deciphering of enzymatic reactions is a proficient advantage over conventional label-based readouts suffering from compound interference and intricate generation of tailored signal mediators. Significant evolvements of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS, as well as associated liquid handling instrumentation, triggered extensive efforts in the drug discovery community to integrate the comprehensive MS readout into the high-throughput screening (HTS) portfolio. Providing speed, sensitivity, and accuracy comparable to those of conventional, label-based readouts, combined with merits of MS-based technologies, such as label-free parallelized measurement of multiple physiological components, emphasizes the advantages of MALDI-TOF for HTS approaches. Here we describe the assay development for the identification of protein tyrosine phosphatase 1B (PTP1B) inhibitors. In the context of this precious drug target, MALDI-TOF was integrated into the HTS environment and cross-compared with the well-established AlphaScreen technology. We demonstrate robust and accurate IC50 determination with high accordance to data generated by AlphaScreen. Additionally, a tailored MALDI-TOF assay was developed to monitor compound-dependent, irreversible modification of the active cysteine of PTP1B. Overall, the presented data proves the promising perspective for the integration of MALDI-TOF into drug discovery campaigns.

A Label-Free Fluorescent AND Logic Gate Aptasensor for Carbohydrate Antigen 15-3 Detection Based on Graphene Oxide

2021 ◽  
Vol 24 ◽  
Author(s):  
Wenxiao Hu ◽  
Yafei Dong ◽  
Luhui Wang ◽  
Yue Wang ◽  
Mengyao Qian ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Logic Gate ◽  
High Sensitivity ◽  
Carbohydrate Antigen ◽  
Fluorescence Signal ◽  
Label Free ◽  
Molecular Logic ◽  
Long Stem ◽  
Middle Ring ◽  
Fluorescent Aptasensor

Background: Molecular logic gate always used fluorescent dyes to realize fluorescence signal. The labeling of the fluorophore is relatively expensive, low yield and singly labeled impuritiesaffects the affinity between the target and the aptamer. Label-free fluorescent aptamer biosensor strategy has attracted widespread interest due to lower cost and simple. Objective: Herein, we have designed a AND logic gate fluorescent aptasensor for detecting carbohydrate antigen 15-3(CA15-3) based on label-free fluorescence signal output. Materials and Methods: A hairpin DNA probe consists of CA15-3 aptamer and partly anti-CA15-3 aptamer sequences as a long stem and G-rich sequences of the middle ring as a quadruplex-forming oligomer. G-rich sequences can fold into a quadruplex by K+, and then G-quadruplex interacts specifically with N-methylmesoporphyrin IX(NMM), leading to a dramatic increase in fluorescence of NMM. With CA15-3 and NMM as the two inputs, the fluorescence intensity of the NMM is the output signal. Lacking of CA15-3 or NMM, there is no significant fluorescence enhancing, and the output of the signal is “0”. The fluorescence signal was dramatically increasing and the output of the signal is “1” only when CA15-3 protein and NMM were added at the same time. Results: This biosensor strategy possessed selectivity, high sensitivity for detecting CA15-3 protein from 10 to 500 U mL-1 and the detection limit was 10 U mL-1, and also showed good reproducibility in spiked human serum. Conclusion: In summary, the proposed AND logic gate fluorescent aptasensor could specifically detect CA15-3.

Realizing an N-two-qubit quantum logic gate in a cavity QED with nearest qubit--qubit interaction

2016 ◽  
Vol 16 (5&6) ◽  
pp. 465-482
Author(s):  
Taoufik Said ◽  
Abdelhaq Chouikh ◽  
Karima Essammouni ◽  
Mohamed Bennai
Keyword(s):  
Quantum Logic ◽  
Cavity Qed ◽  
Logic Gate ◽  
Operation Time ◽  
Logic Gates ◽  
Initial State ◽  
Gate Operation ◽  
Quantum Logic Gates ◽  
Current State ◽  
Phase Gate

We propose an effective way for realizing a three quantum logic gates (NTCP gate, NTCP-NOT gate and NTQ-NOT gate) of one qubit simultaneously controlling N target qubits based on the qubit-qubit interaction. We use the superconducting qubits in a cavity QED driven by a strong microwave field. In our scheme, the operation time of these gates is independent of the number N of qubits involved in the gate operation. These gates are insensitive to the initial state of the cavity QED and can be used to produce an analogous CNOT gate simultaneously acting on N qubits. The quantum phase gate can be realized in a time (nanosecond-scale) much smaller than decoherence time and dephasing time (microsecond-scale) in cavity QED. Numerical simulation under the influence of the gate operations shows that the scheme could be achieved efficiently within current state-of-the-art technology.

Logical Chaotic Resonance in a Bistable System

2020 ◽  
Vol 30 (13) ◽  
pp. 2050196 ◽  
Author(s):  
Yuangen Yao ◽  
Jun Ma
Keyword(s):  
Signal Intensity ◽  
First Passage Time ◽  
Logic Gate ◽  
Response Speed ◽  
Chaotic Signal ◽  
Passage Time ◽  
Bistable System ◽  
Gate Operation ◽  
Intuitive Interpretation

In this work, we demonstrate a new chaotic signal-induced phenomenon that the output of a chaotic signal-driven bistable system can be consistently mapped to specific logic gate operation in an optimal window of chaotic signal intensity. We term this phenomenon logical chaotic resonance (LCR). Then, an intuitive interpretation for LCR phenomenon is given based on potential well map and mean first-passage time. Through LCR mechanism, the chaotic signal with proper intensity is used to obtain reliable logical gate in the bistable system. Besides, appropriately increasing the chaotic signal intensity can effectively improve the response speed of the bistable system to the change of input signal. Finally, the role of chaotic signal in enhancing the capacity of resisting disturbance of parameters is demonstrated.

Carbon nanodots prepared for dopamine and Al3+ sensing, cellular imaging and logic gate operation

2016 ◽  
Vol 68 ◽  
pp. 732-738 ◽  
Author(s):  
Fanyong Yan ◽  
Depeng Kong ◽  
Yunmei Luo ◽  
Qianghua Ye ◽  
Yinyin Wang ◽  
...  
Keyword(s):  
Logic Gate ◽  
Cellular Imaging ◽  
Carbon Nanodots ◽  
Gate Operation

An “on–off–on” fluorescent nanoprobe for recognition of Cu2+ and GSH based on nitrogen co-doped carbon quantum dots, and its logic gate operation

2019 ◽  
Vol 11 (20) ◽  
pp. 2650-2657 ◽  
Author(s):  
Yifang Gao ◽  
Huilin Zhang ◽  
Shaomin Shuang ◽  
Hui Han ◽  
Chuan Dong
Keyword(s):  
Quantum Dots ◽  
Carbon Dots ◽  
Logic Gate ◽  
Carbon Quantum Dots ◽  
Gate Operation ◽  
Fluorescent Nanoprobe ◽  
Highly Sensitive ◽  
Doped Carbon Dots ◽  
Co Doped

Novel nitrogen co-doped carbon dots (NCDs) were synthesized as a fluorescent “on–off–on” switch for the highly sensitive and selective sensing of Cu2+ and glutathione (GSH) by a straightforward pyrolysis route.

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