Effects of tonic immobility (TI) and corticosterone (CORT) on energy status and protein metabolism in pectoralis major muscle of broiler chickens

2014 ◽  
Vol 169 ◽  
pp. 90-95 ◽  
Author(s):  
Yujing Duan ◽  
Wenyan Fu ◽  
Song Wang ◽  
Yingdong Ni ◽  
Ruqian Zhao
Keyword(s):  
Protein Metabolism ◽  
Broiler Chickens ◽  
Tonic Immobility ◽  
Pectoralis Major Muscle ◽  
Pectoralis Major ◽  
Energy Status

Effects of tonic immobility and corticosterone on mitochondria metabolism in pectoralis major muscle of broiler chickens

2018 ◽  
Vol 58 (12) ◽  
pp. 2192
Author(s):  
Yujing Duan ◽  
Lili Sun ◽  
Jie Liu ◽  
Wenyan Fu ◽  
Song Wang ◽  
...  
Keyword(s):  
Protein Level ◽  
Copy Number ◽  
Broiler Chickens ◽  
Energy Homeostasis ◽  
Tonic Immobility ◽  
Pectoralis Major Muscle ◽  
Pectoralis Major ◽  
Mtdna Copy Number ◽  
Muscle Weight ◽  
Significant Difference

Tonic immobility (TI), which can be divided into short (STI) or long (LTI) duration, is a trait related to fear and stress response. In a previous study, we found that in broilers that LTI phenotype and chronic corticosterone (CORT) administration caused retarded growth and lower muscle weight compared with their control counterparts. The aim of this study is to determine whether the mitochondrial DNA (mtDNA) copy number and mitochondrial oxidative phosphorylation (OXPHOS), the vital factors involved in regulating energy homeostasis, have been changed by LTI or CORT treatment. The results showed that STI broilers had higher mtDNA copy number and cytochrome c oxidase (COX) enzyme activity compared with LTI broilers. Analysis of mtDNA-encoded OXPHOS genes revealed that the mRNA expression of the COX subunit 1, 2, NADH dehydrogenase (ND) subunits 1, 3 and 6, were also increased in STI broilers compared with LTI broilers. Regarding the transcriptional regulation of mtDNA-encoded OXPHOS genes, no difference was found in the methylation of the mitochondria control region between the TI phenotypes or the CORT treatments. The PGC-1α protein level was higher in STI broilers, but the av uncoupling proteins, did not show significant difference at the protein level between TI phenotypes. These results suggest that the mitochondrial function in pectoralis major muscle of STI broilers is better than that of LTI counterparts. However, chronic CORT administration did not affect the mitochondrial metabolism, indicating the mitochondrial insensitivity to CORT treatment in pectoralis major muscle.

scholarly journals Nucleotides in broiler chicken diet: effect on breast muscles quality

2011 ◽  
Vol 29 (No. 4) ◽  
pp. 308-317 ◽  
Author(s):  
B. Chiofalo ◽  
V. Lo Presti ◽  
G. Samoini ◽  
D. Alessandro E ◽  
V. Chiofalo ◽  
...  
Keyword(s):  
Shear Force ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Pectoralis Major Muscle ◽  
Dietary Supplementation ◽  
Pectoralis Major ◽  
Atherogenic Index ◽  
Homogeneous Groups ◽  
Lower Shear ◽  
Nutritional Characteristics

The study evaluated the effects of nucleotide dietary supplementation on the physical and nutritional characteristics of the Pectoralis major muscle of male broiler chickens (n = 60 000), divided into two homogeneous groups: Control (C) and Nucleotides (N). The animals of the two groups, from the birth (24 h of age) to the slaughtering age (52 days), received the same diet, supplemented (N) or not (C) with 0.1% of a Nucleotide pool. At the slaughtering, on a sample of 130 animals per group, randomly selected, the physical and nutritional characteristics of Pectoralis major muscle were determined. The meat of the N group showed significantly higher redness and Hue values, lower shear force values, higher lipid and ash percentages and iron content. Moreover, nucleotides significantly increased monounsaturated acids and linolenic acid and decreased eicosapentanoic and docosahexanoic acids. The unsaturation degree was higher in the Nucleotides group and Atherogenic index was positively influenced by the nucleotide supplementation. Nucleotide dietary supplementation improved the physical and nutritional characteristics of the Pectoralis major muscle of broiler chickens.

scholarly journals Ectopic Lipid Accumulation is Key Feature in Early Stages of Wooden Breast

2019 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Expression Analysis ◽  
Lipid Accumulation ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from a subset of 101 birds randomly selected from a total of 302 birds at 14 days of age, after which all birds were raised to 7 weeks of age for scoring of wooden breast. RNA sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

scholarly journals Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 746 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Lipid Metabolism ◽  
Expression Analysis ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from 101 birds at 14 days of age. Birds were subsequently raised to 7 weeks of age to allow sample selection based on the wooden breast phenotype at market age. RNA-sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p-value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

scholarly journals Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

2019 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Lipid Metabolism ◽  
Expression Analysis ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from 101 birds at 14 days of age. Birds were subsequently raised to 7 weeks of age to allow sample selection based on the wooden breast phenotype at market age. RNA sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

Performance, diameter of muscle fibers, and gene expression of mechanistic target of rapamycin in pectoralis major muscle of broilers supplemented with leucine and valine

2019 ◽  
Vol 99 (1) ◽  
pp. 168-178 ◽  
Author(s):  
Iván Camilo Ospina-Rojas ◽  
Alice Eiko Murakami ◽  
Cristiane Regina do Amaral Duarte ◽  
Paulo Cesar Pozza ◽  
Robson Marcelo Rossi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Muscle Fiber ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Pectoralis Major ◽  
Target Of Rapamycin ◽  
Leucine Supplementation ◽  
Mechanistic Target Of Rapamycin ◽  
Protein Diets

Two experiments were performed to study the interaction between the standardized ileal digestible (SID) leucine (Leu) and valine (Val) levels on the mRNA expression of genes involved in the mechanistic target of rapamycin (mTOR) pathway (experiment I) and determine the requirement of these amino acids in low-protein diets, and their effects on performance, serum parameters and muscle fiber diameters of broilers (experiment II) from day 1 to day 21 post hatch. Broiler chickens were distributed in a completely randomized design in a 3 × 3 and 5 × 5 factorial arrangement for a total of 9 and 25 treatments in experiments I and II, respectively. There was no (P > 0.05) interaction between the SID Leu and Val levels on mRNA expression of mTOR, S6 kinase 1 (S6K1), 4E-binding protein-1 (4EBP1), eukaryotic elongation factor 2 (eEF2), and insulin-like growth factor-1 (IGF-1) genes in pectoralis major muscle. Leucine supplementation increased (P < 0.05) mRNA expression of mTOR and S6K1 genes in muscle tissue, whereas Val supplementation did not affect (P > 0.05) mRNA expression of the genes investigated. Interaction was observed (P < 0.05) between dietary Leu and Val levels on feed intake and gain:feed. Leucine supplementation may stimulate mRNA expression of mTOR and S6K1 genes in pectoralis major muscle of broilers from day 1 to day 21 post hatch. The SID Leu and Val levels required for the optimization of feed intake, weight gain, and gain:feed in low-crude protein diets for broiler chickens from day 1 to 21 post hatch were estimated at 1.29% and 0.96%, 1.28% and 0.92%, and 1.27% and 0.91%, respectively; however, these requirements may be greater to maximize muscle fiber growth.

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