Characterization of six small HSP genes from Chironomus riparius (Diptera, Chironomidae): Differential expression under conditions of normal growth and heat-induced stress

2015 ◽  
Vol 188 ◽  
pp. 76-86 ◽  
Author(s):  
Raquel Martín-Folgar ◽  
Mercedes de la Fuente ◽  
Gloria Morcillo ◽  
José-Luis Martínez-Guitarte
Keyword(s):  
Differential Expression ◽  
Normal Growth ◽  
Chironomus Riparius ◽  
Induced Stress ◽  
Small Hsp ◽  
Hsp Genes

scholarly journals Genome-Wide Identification and Characterization of Hsf and Hsp Gene Families and Gene Expression Analysis under Heat Stress in Eggplant (Solanum melongema L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. 149
Author(s):  
Chao Gong ◽  
Qiangqiang Pang ◽  
Zhiliang Li ◽  
Zhenxing Li ◽  
Riyuan Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
Gene Families ◽  
High Temperature Stress ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Genome Wide ◽  
Motif Composition ◽  
Hsp Genes ◽  
Identification And Characterization

Under high temperature stress, a large number of proteins in plant cells will be denatured and inactivated. Meanwhile Hsfs and Hsps will be quickly induced to remove denatured proteins, so as to avoid programmed cell death, thus enhancing the thermotolerance of plants. Here, a comprehensive identification and analysis of the Hsf and Hsp gene families in eggplant under heat stress was performed. A total of 24 Hsf-like genes and 117 Hsp-like genes were identified from the eggplant genome using the interolog from Arabidopsis. The gene structure and motif composition of Hsf and Hsp genes were relatively conserved in each subfamily in eggplant. RNA-seq data and qRT-PCR analysis showed that the expressions of most eggplant Hsf and Hsp genes were increased upon exposure to heat stress, especially in thermotolerant line. The comprehensive analysis indicated that different sets of SmHsps genes were involved downstream of particular SmHsfs genes. These results provided a basis for revealing the roles of SmHsps and SmHsp for thermotolerance in eggplant, which may potentially be useful for understanding the thermotolerance mechanism involving SmHsps and SmHsp in eggplant.

Characterization of Hsp70 gene in Chironomus riparius: Expression in response to endocrine disrupting pollutants as a marker of ecotoxicological stress

2011 ◽  
Vol 153 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Mónica Morales ◽  
Rosario Planelló ◽  
Pedro Martínez-Paz ◽  
Oscar Herrero ◽  
Estrella Cortés ◽  
...  
Keyword(s):  
Chironomus Riparius ◽  
Hsp70 Gene ◽  
Endocrine Disrupting

scholarly journals Identification and Characterization of Chalcone Isomerase Genes Involved in Flavonoid Production in Dracaena cambodiana

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiahong Zhu ◽  
Wan Zhao ◽  
Rongshuang Li ◽  
Dong Guo ◽  
Huiliang Li ◽  
...  
Keyword(s):  
Transcriptional Profiling ◽  
Normal Growth ◽  
Growth Conditions ◽  
Ultraviolet B ◽  
Chalcone Isomerase ◽  
Enzyme Activity Assay ◽  
Dragon’S Blood ◽  
Dracaena Cambodiana ◽  
Key Enzyme

Dragon’s blood is a traditional medicine in which flavonoids are the main bioactive compounds; however, the underlying formation mechanism of dragon’s blood remains largely poorly understood. Chalcone isomerase (CHI) is the key enzyme in the flavonoid biosynthesis pathway. However, CHI family genes are not well understood in Dracaena cambodiana Pierre ex Gagnep, an important source plant of dragon’s blood. In this study, 11 CHI family genes were identified from D. cambodiana, and they were classified into three types. Evolutionary and transcriptional profiling analysis revealed that DcCHI1 and DcCHI4 might be involved in flavonoid production. Both DcCHI1 and DcCHI4 displayed low expression levels in stem under normal growth conditions and were induced by methyl jasmonate (MeJA), 6-benzyl aminopurine (6-BA, synthetic cytokinin), ultraviolet-B (UV-B), and wounding. The recombinant proteins DcCHI1 and DcCHI4 were expressed in Escherichia coli and purified by His-Bind resin chromatography. Enzyme activity assay indicated that DcCHI1 catalyzed the formation of naringenin from naringenin chalcone, while DcCHI4 lacked this catalytic activity. Overexpression of DcCHI1 or DcCHI4 enhanced the flavonoid production in D. cambodiana and tobacco. These findings implied that DcCHI1 and DcCHI4 play important roles in flavonoid production. Thus, our study will not only contribute to better understand the function and expression regulation of CHI family genes involved in flavonoid production in D. cambodiana but also lay the foundation for developing the effective inducer of dragon’s blood.

Characterization of Thermally Induced Stress in IC Packages Using PiFETs Over a Temperature Range of −180°C to 80°C

2014 ◽  
Vol 2014 (1) ◽  
pp. 000500-000504 ◽  
Author(s):  
Francy J. Akkara ◽  
Uday S. Goteti ◽  
Richard C. Jaeger ◽  
Michael C. Hamilton ◽  
Michael J. Palmer ◽  
...  
Keyword(s):  
Shear Stress ◽  
Stress Component ◽  
Shear Stress Component ◽  
Thermally Induced ◽  
Temperature Changes ◽  
Temperature Range ◽  
Induced Stress ◽  
Highly Sensitive ◽  
Stress Components

In certain applications, IC packages may be exposed to extreme temperatures and knowledge of thermally induced stress aids the prediction of performance degradation or failure of the IC. In the devices that are used in extreme conditions, the stress is caused mainly by the mismatch in expansion of various materials triggered by the different coefficients of thermal expansion. This work performed in this study is conducted using NMOS current mirror circuits that are cycled through a wide temperature range of −180°C to 80°C. These circuits are highly sensitive to stress and provide well-localized measurements of shear stress. The sensors are fabricated in such a way that the effects of certain stress components are isolated. These sensors are also temperature compensated so that only the effect of mechanical stress components is observed and changes in device performance due to temperature changes are minimal. Current readings obtained from the sensors are used to extract the shear stress component. Finite element simulations, using expected materials performance parameter information were also performed for similar packages and these results are compared to the measured results.

Characterization of a 70Z/3 pre-B cell derived macrophage clone. Differential expression of Hox family genes

1990 ◽  
Vol 2 (8) ◽  
pp. 691-696 ◽  
Author(s):  
Hindeo Hara ◽  
Mehran Sam ◽  
Richard A. Maki ◽  
Gillian E. Wu ◽  
Christopher J. Paige
Keyword(s):  
B Cell ◽  
Differential Expression

Characterization of Deformation and Wear Mechanisms During Indentation Scratching on Pure Zinc

2019 ◽  
Vol 142 (1) ◽  
Author(s):  
Aditya Gokhale ◽  
Jayant Jain ◽  
Rajesh Prasad ◽  
E-Wen Huang ◽  
Soo Yeol Lee
Keyword(s):  
Wear Mechanism ◽  
Material Removal ◽  
Scratch Testing ◽  
Pure Zinc ◽  
Micro Cutting ◽  
Induced Stress ◽  
Surface Examination ◽  
Strain Hardening Behavior ◽  
Number Of Passes

Abstract Scratch testing using nanoindentation helps to characterize material’s wear resistance and the accompanying material removal, and deformation mechanism. The effect of load and repeated scratching on pure zinc was investigated in this study. Surface examination post scratching revealed the formation of long chips at lower loads, which can be attributed to ductile-like behavior. Ploughing dominated at higher loads, while micro-cutting was active at lower loads. The material removal factor (fab) is an indicator of the prevailing wear mechanism. A good correlation appeared between the observed wear mechanism and the calculated fab, wherein interplay of ploughing and micro-cutting was observed. The decrease in wear rate as the number of passes increased and the decreasing value of fab are indicative of the strain hardening behavior during repeated scratching. The presence of intergranular fracture slip and twinning on the deformed surface is indicative of the material’s response to induced stress.

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