Use of strontium doping glass-ceramic material for bone regeneration in critical defect: In vitro and in vivo analyses

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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In vitro and in vivo Evaluation of BMSC Laden RGD-Conjugated MPEG-PCL Composite Hydrogels for Bone Regeneration

SSRN Electronic Journal ◽

10.2139/ssrn.3447004 ◽

2019 ◽

Author(s):

Hyun Joo Kim ◽

Su Jung You ◽

Dae Hyeok Yang ◽

Heung Jae Chun ◽

Hae Kwan Park ◽

...

Keyword(s):

Bone Regeneration ◽

Composite Hydrogels

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In Vitro and In Vivo Study of a Novel Nanoscale Demineralized Bone Matrix Coated PCL/β‐TCP Scaffold for Bone Regeneration

Macromolecular Bioscience ◽

10.1002/mabi.202000336 ◽

2020 ◽

pp. 2000336

Author(s):

Bo Yuan ◽

Zhiwei Wang ◽

Yin Zhao ◽

Yifan Tang ◽

Shengyuan Zhou ◽

...

Keyword(s):

Bone Regeneration ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

In Vivo Study ◽

Demineralized Bone

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Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration

International Journal of Oral Science ◽

10.1038/s41368-021-00132-6 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Lu Wang ◽

Shuwei Liu ◽

Chunxia Ren ◽

Siyuan Xiang ◽

Daowei Li ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Defect ◽

Load Capacity ◽

Good Prospect ◽

Alizarin Red ◽

Drug Load ◽

Load Rate ◽

Low Toxicity

AbstractNanomaterial-based drug sustainable release systems have been tentatively applied to bone regeneration. They, however, still face disadvantages of high toxicity, low biocompatibility, and low drug-load capacity. In view of the low toxicity and high biocompatibility of polymer nanomaterials and the excellent load capacity of hollow nanomaterials with high specific surface area, we evaluated the hollow polydopamine nanoparticles (HPDA NPs), in order to find an optimal system to effectively deliver the osteogenic drugs to improve treatment of bone defect. Data demonstrated that the HPDA NPs synthesized herein could efficiently load four types of osteogenic drugs and the drugs can effectively release from the HPDA NPs for a relatively longer time in vitro and in vivo with low toxicity and high biocompatibility. Results of qRT-PCR, ALP, and alizarin red S staining showed that drugs released from the HPDA NPs could promote osteogenic differentiation and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) in vitro. Image data from micro-CT and H&E staining showed that all four osteogenic drugs released from the HPDA NPs effectively promoted bone regeneration in the defect of tooth extraction fossa in vivo, especially tacrolimus. These results suggest that the HPDA NPs, the biodegradable hollow polymer nanoparticles with high drug load rate and sustainable release ability, have good prospect to treat the bone defect in future clinical practice.

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Stromal cell‐derived factor‐1/Exendin‐4 cotherapy facilitates the proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells in vitro and promotes periodontal bone regeneration in vivo

Cell Proliferation ◽

10.1111/cpr.12997 ◽

2021 ◽

Author(s):

Qianyu Liang ◽

Lingqian Du ◽

Rui Zhang ◽

Wenyan Kang ◽

Shaohua Ge

Keyword(s):

Stem Cells ◽

Bone Regeneration ◽

Osteogenic Differentiation ◽

Periodontal Ligament ◽

Stromal Cell ◽

Periodontal Ligament Stem Cells ◽

Stromal Cell Derived Factor

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Three-Dimensional Zirconia-Based Scaffolds for Load-Bearing Bone-Regeneration Applications: Prospects and Challenges

Materials ◽

10.3390/ma14123207 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3207

Author(s):

Kumaresan Sakthiabirami ◽

Vaiyapuri Soundharrajan ◽

Jin-Ho Kang ◽

Yunzhi Peter Yang ◽

Sang-Won Park

Keyword(s):

Bone Regeneration ◽

Biological Activities ◽

Three Dimensional ◽

In Vivo Studies ◽

High Mechanical Strength ◽

Real World Applications ◽

3D Fabrication ◽

Dental Applications

The design of zirconia-based scaffolds using conventional techniques for bone-regeneration applications has been studied extensively. Similar to dental applications, the use of three-dimensional (3D) zirconia-based ceramics for bone tissue engineering (BTE) has recently attracted considerable attention because of their high mechanical strength and biocompatibility. However, techniques to fabricate zirconia-based scaffolds for bone regeneration are in a stage of infancy. Hence, the biological activities of zirconia-based ceramics for bone-regeneration applications have not been fully investigated, in contrast to the well-established calcium phosphate-based ceramics for bone-regeneration applications. This paper outlines recent research developments and challenges concerning numerous three-dimensional (3D) zirconia-based scaffolds and reviews the associated fundamental fabrication techniques, key 3D fabrication developments and practical encounters to identify the optimal 3D fabrication technique for obtaining 3D zirconia-based scaffolds suitable for real-world applications. This review mainly summarized the articles that focused on in vitro and in vivo studies along with the fundamental mechanical characterizations on the 3D zirconia-based scaffolds.

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The Bone Regeneration Capacity of BMP-2 + MMP-10 Loaded Scaffolds Depends on the Tissue Status

Pharmaceutics ◽

10.3390/pharmaceutics13070979 ◽

2021 ◽

Vol 13 (7) ◽

pp. 979

Author(s):

Patricia Garcia-Garcia ◽

Ricardo Reyes ◽

José Antonio Rodriguez ◽

Tomas Martín ◽

Carmen Evora ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Growth Promotion ◽

Tissue Growth ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein ◽

Therapeutic Molecules ◽

Positive Effect

Biomaterials-mediated bone formation in osteoporosis (OP) is challenging as it requires tissue growth promotion and adequate mineralization. Based on our previous findings, the development of scaffolds combining bone morphogenetic protein 2 (BMP-2) and matrix metalloproteinase 10 (MMP-10) shows promise for OP management. To test our hypothesis, scaffolds containing BMP-2 + MMP-10 at variable ratios or BMP-2 + Alendronate (ALD) were prepared. Systems were characterized and tested in vitro on healthy and OP mesenchymal stem cells and in vivo bone formation was studied on healthy and OP animals. Therapeutic molecules were efficiently encapsulated into PLGA microspheres and embedded into chitosan foams. The use of PLGA (poly(lactic-co-glycolic acid)) microspheres as therapeutic molecule reservoirs allowed them to achieve an in vitro and in vivo controlled release. A beneficial effect on the alkaline phosphatase activity of non-OP cells was observed for both combinations when compared with BMP-2 alone. This effect was not detected on OP cells where all treatments promoted a similar increase in ALP activity compared with control. The in vivo results indicated a positive effect of the BMP-2 + MMP-10 combination at both of the doses tested on tissue repair for OP mice while it had the opposite effect on non-OP animals. This fact can be explained by the scaffold’s slow-release rate and degradation that could be beneficial for delayed bone regeneration conditions but had the reverse effect on healthy animals. Therefore, the development of adequate scaffolds for bone regeneration requires consideration of the tissue catabolic/anabolic balance to obtain biomaterials with degradation/release behaviors suited for the existing tissue status.

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Potential Implantable Nanofibrous Biomaterials Combined with Stem Cells for Subchondral Bone Regeneration

Materials ◽

10.3390/ma13143087 ◽

2020 ◽

Vol 13 (14) ◽

pp. 3087

Author(s):

Rana Smaida ◽

Luc Pijnenburg ◽

Silvia Irusta ◽

Erico Himawan ◽

Gracia Mendoza ◽

...

Keyword(s):

Stem Cells ◽

Bone Regeneration ◽

Subchondral Bone ◽

Therapeutic Potential ◽

Sodium Hyaluronate ◽

Vinyl Pyrrolidone ◽

Regenerative Ability ◽

Poly Vinyl Pyrrolidone

The treatment of osteochondral defects remains a challenge. Four scaffolds were produced using Food and Drug Administration (FDA)-approved polymers to investigate their therapeutic potential for the regeneration of the osteochondral unit. Polycaprolactone (PCL) and poly(vinyl-pyrrolidone) (PVP) scaffolds were made by electrohydrodynamic techniques. Hydroxyapatite (HAp) and/or sodium hyaluronate (HA) can be then loaded to PCL nanofibers and/or PVP particles. The purpose of adding hydroxyapatite and sodium hyaluronate into PCL/PVP scaffolds is to increase the regenerative ability for subchondral bone and joint cartilage, respectively. Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were seeded on these biomaterials. The biocompatibility of these biomaterials in vitro and in vivo, as well as their potential to support MSC differentiation under specific chondrogenic or osteogenic conditions, were evaluated. We show here that hBM-MSCs could proliferate and differentiate both in vitro and in vivo on these biomaterials. In addition, the PCL-HAp could effectively increase the mineralization and induce the differentiation of MSCs into osteoblasts in an osteogenic condition. These results indicate that PCL-HAp biomaterials combined with MSCs could be a beneficial candidate for subchondral bone regeneration.

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Matrix Vesicles: Role in Bone Mineralization and Potential Use as Therapeutics

Pharmaceuticals ◽

10.3390/ph14040289 ◽

2021 ◽

Vol 14 (4) ◽

pp. 289

Author(s):

Sana Ansari ◽

Bregje W. M. de de Wildt ◽

Michelle A. M. Vis ◽

Carolina E. de de Korte ◽

Keita Ito ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Bone Cells ◽

Bone Mineralization ◽

Recipient Cell ◽

Organic Matrix ◽

Matrix Vesicles ◽

Matrix Mineralization

Bone is a complex organ maintained by three main cell types: osteoblasts, osteoclasts, and osteocytes. During bone formation, osteoblasts deposit a mineralized organic matrix. Evidence shows that bone cells release extracellular vesicles (EVs): nano-sized bilayer vesicles, which are involved in intercellular communication by delivering their cargoes through protein–ligand interactions or fusion to the plasma membrane of the recipient cell. Osteoblasts shed a subset of EVs known as matrix vesicles (MtVs), which contain phosphatases, calcium, and inorganic phosphate. These vesicles are believed to have a major role in matrix mineralization, and they feature bone-targeting and osteo-inductive properties. Understanding their contribution in bone formation and mineralization could help to target bone pathologies or bone regeneration using novel approaches such as stimulating MtV secretion in vivo, or the administration of in vitro or biomimetically produced MtVs. This review attempts to discuss the role of MtVs in biomineralization and their potential application for bone pathologies and bone regeneration.

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