1H-NMR based lipid profiling of Gossypium hirsutum seed oil at different developmental stages

Abstract • Background • Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. • Results • In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5,333 differentially expressed genes (DEG) with 2,534 upregulated and 2,799 downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. • Conclusions • This comparative analysis of NEC and EC transcriptomes gives new insights into the genetic underpinnings of somatic embryogenesis in cotton.

Download Full-text

1H NMR and GC-MS metabolic fingerprinting of developmental stages of Rhizoctonia solani sclerotia

Metabolomics ◽

10.1007/s11306-009-0180-4 ◽

2009 ◽

Vol 6 (1) ◽

pp. 96-108 ◽

Cited By ~ 28

Author(s):

K. A. Aliferis ◽

S. Jabaji

Keyword(s):

Rhizoctonia Solani ◽

1H Nmr ◽

Developmental Stages ◽

Metabolic Fingerprinting

Download Full-text

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)

BMC Developmental Biology ◽

10.1186/s12861-020-00230-4 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Li Wen ◽

Wei Li ◽

Stephen Parris ◽

Matthew West ◽

John Lawson ◽

...

Keyword(s):

Gene Expression ◽

Somatic Embryogenesis ◽

Transcription Factors ◽

Gossypium Hirsutum ◽

Embryogenic Callus ◽

Developmental Stages ◽

Baby Boom ◽

Expression Profiles ◽

Study Gene Expression ◽

Ec Cells

Abstract Background Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. The critical genetic architecture of non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells in a highly regenerable cotton genotype is unknown. Results In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5333 differentially expressed genes (DEG) with 2534 genes upregulated and 2799 genes downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes were unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. Conclusions This comparative analysis of NEC and EC transcriptomes gives new insights into the genes involved in somatic embryogenesis in cotton.

Download Full-text

Effects of Simulated MSMA Drift on Rice (Oryza sativa) Growth and Yield

Weed Science ◽

10.1017/s0043174500062007 ◽

1981 ◽

Vol 29 (3) ◽

pp. 303-308 ◽

Cited By ~ 13

Author(s):

E. P. Richard ◽

H. R. Hurst ◽

R. D. Wauchope

Keyword(s):

Oryza Sativa ◽

Gossypium Hirsutum ◽

Plant Height ◽

Weed Control ◽

Developmental Stages ◽

Growth And Yield ◽

Stages Of Development ◽

Early Season ◽

Application Timing ◽

Over The Top

Levels of MSMA (monosodium methanearsonate) that simulated drift (0.2, 0.39, and 0.78 kg/ha) were applied over-the-top to rice (Oryza sativaL. ‘Vista’, ‘Starbonnet’, or ‘Labelle’) after flooding at four developmental stages between early tillering and late jointing. The MSMA rates used were equivalent to, or lower than, the 0.78-kg/ha rate that would be applied as an over-the-top application to cotton (Gossypium hirsutumL.) for early season weed control. Over-the-top applications of MSMA did not affect heading and maturity dates of filled grains regardless of the rate or application timing. Reductions occurred, however, in plant height, number of panicles, and yield. In addition, the percentage of erect panicles showing typical “straighthead” symptoms increased. Rice sensitivity to MSMA depended on MSMA rate and stage of growth with injury being most severe as rice approached the reproductive stages of development. Because aerial applications of MSMA to cotton are normally made before rice reaches the reproductive stage, injury resulting from MSMA drift should be minimal.

Download Full-text