Early growth reduction in Vicia faba L. under alkali salt stress is mainly caused by excess bicarbonate and related to citrate and malate over accumulation

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

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Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)

Agronomy ◽

10.3390/agronomy11030547 ◽

2021 ◽

Vol 11 (3) ◽

pp. 547

Author(s):

Arafat Abdel Hamed Abdel Latef ◽

Md. Tahjib-Ul-Arif ◽

Mohammad Saidur Rhaman

Keyword(s):

Salt Stress ◽

Vicia Faba ◽

Faba Bean ◽

Stress Responses ◽

Foliar Application ◽

Soluble Sugar ◽

Principal Component ◽

Vicia Faba L ◽

Catalase Peroxidase ◽

Amino Acid Contents

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.

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Effect of Salt Stress and Bentonite on the Germination and Proline Content of Vicia faba L. Plant var. ‘Semilla violeta’ and ‘Reine mora’

American Journal of Plant Physiology ◽

10.3923/ajpp.2012.212.219 ◽

2012 ◽

Vol 7 (5) ◽

pp. 212-219

Author(s):

H.A. Reguieg Yssaad ◽

A. Latigui ◽

T. Nouri ◽

L. Bessafi

Keyword(s):

Salt Stress ◽

Vicia Faba ◽

Proline Content ◽

Vicia Faba L

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Effect The impact of salt stress on the growth and photosynthetic pigments of Vicia faba (L).

Scientific Journal for Faculty of Science-Sirte University ◽

10.37375/sjfssu.v1i2.61 ◽

2021 ◽

Vol 1 (2) ◽

pp. 32-40

Keyword(s):

Salt Stress ◽

Vicia Faba ◽

Photosynthetic Pigments ◽

Irrigation Water ◽

Sea Water ◽

Saline Irrigation ◽

Horticultural Crops ◽

Daily Increments ◽

Vicia Faba L ◽

The Impact

Water used for crop irrigation is often of insufficient quality in the Mediterranean region, where sea water invades the porous karst matrix and salinizes Sources of soil and water. Measuring the productivity of horticultural crops under saline conditions helps decide if and when crops should be irrigated if water is saline, thus balancing crop water and salt stress. To examine the effects of saline irrigation water on (Vicia faba L.) biomass and yield parameters, a greenhouse pot experiment was set up.NaCl salinity was applied as follows: After 15 days from irrigation, plants were subjected to graded levels of salinity: 0, 50, 100, 200 and 300 mM NaCl, applied in stepwise daily increments to avoid induction of salt shock to the seedlings. Height of Plant (cm), The number of lateral branches , number of( leaves, pods, seeds) per plant, shoot weight (g), is correlated with an increase in stressed plant photosynthetic pigments. five weeks after salinity therapy, the weight of the pod (g) and the weight of the seed (g) were determined. Increased irrigation water salinity statistically significantly decreased the calculated parameters (P<0.05) relative to regulation , except for the number of branches and pods. Salinity is associated with an increase in photosynthetic pigments in stressed plants. The productivity of Vicia faba has decreased in proportion to the degree of salinity of irrigation water.

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