Microencapsulation of Lactobacillus rhamnosus using sesame protein isolate: Effect of encapsulation method and transglutaminase

2021 ◽  
Vol 41 ◽  
pp. 101012
Author(s):  
Saeedeh Azizi ◽  
Mahmoud Rezazadeh-Bari ◽  
Hadi Almasi ◽  
Saber Amiri
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Protein Isolate ◽  
Encapsulation Method

scholarly journals Development of milk protein edible films incorporated with Lactobacillus rhamnosus GG

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6960-6973
Author(s):  
Yun Ying Lee ◽  
Elaine ◽  
Yus Aniza Yusof ◽  
Liew Phing Pui
Keyword(s):  
Storage Temperature ◽  
Lactobacillus Rhamnosus ◽  
Sodium Caseinate ◽  
Edible Films ◽  
Protein Isolate ◽  
Drying Process ◽  
Lactobacillus Rhamnosus Gg ◽  
Film Forming ◽  
Puncture Force ◽  
And Storage

Bioactive edible films have the potential to be probiotic carriers. This innovative approach can replace plastic packaging and can benefit human health. This study demonstrated the incorporation of Lactobacillus rhamnosus GG (LGG) into whey protein isolate (WPI) and sodium caseinate (NaCas) edible films. Probiotic cells were directly incorporated into the film forming solutions, and the films were produced by the casting method. The physical, mechanical, and probiotic viability properties of the edible films were determined in the presence and absence of LGG. Furthermore, the viability of LGG was evaluated during the drying process and storage of 14 days at 4 °C and 25 °C, respectively. The results showed the incorporation of LGG increased the moisture content, puncture force, and lightness of both films. However, viability of LGG was lower in the WPI film regardless of storage temperature. At the end of storage days, both WPI and NaCas edible films maintained the LGG viability above the recommended levels when stored at 4 °C, which was 106 CFU/g. The findings of this study suggested that edible films made of WPI and NaCas showed feasibility to immobilize LGG with chilled storage at 4 ℃.

scholarly journals Statistical optimization of bambara groundnut protein isolate-alginate matrix systems on survival of encapsulated Lactobacillus rhamnosus GG

2017 ◽  
Vol 3 (4) ◽  
pp. 713-732
Author(s):  
Kanyanat Kaewiad ◽  
◽  
Nattha Kaewnopparat ◽  
Damrongsak Faroongsarng ◽  
Juraithip Wungsintaweekul ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Protein Isolate ◽  
Bambara Groundnut ◽  
Statistical Optimization ◽  
Alginate Matrix

Maillard conjugates of whey protein isolate–xylooligosaccharides for the microencapsulation of Lactobacillus rhamnosus: protective effects and stability during spray drying, storage and gastrointestinal digestion

2021 ◽  
Author(s):  
Yang Liao ◽  
Yu Hu ◽  
Nan Fu ◽  
Juwu Hu ◽  
Hua Xiong ◽  
...  
Keyword(s):  
Whey Protein ◽  
Spray Drying ◽  
Lactobacillus Rhamnosus ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Maillard Reaction Products ◽  
Protective Effects ◽  
Reaction Products ◽  
Gastrointestinal Digestion ◽  
Heat Method

The Maillard reaction products (MRPs) of whey protein isolate (WPI) and xylooligosaccharides (XOS) were prepared by a moist heat method for use as protectants to encapsulate Lactobacillus rhamnosus via spray drying.

Microencapsulation of Lactobacillus rhamnosus ATCC 7469 in whey protein isolate-crystalline nanocellulose-inulin composite enhanced gastrointestinal survivability

LWT ◽  
2020 ◽  
Vol 126 ◽  
pp. 109224 ◽  
Author(s):  
Omid Maleki ◽  
Mohammad Alizadeh Khaledabad ◽  
Saber Amiri ◽  
Asghar Khosrowshahi Asl ◽  
Sina Makouie
Keyword(s):  
Whey Protein ◽  
Lactobacillus Rhamnosus ◽  
Whey Protein Isolate ◽  
Protein Isolate

Interactions between Lactobacillus rhamnosus GG and whey protein isolate for the spray-drying preservation of bacterial viability

2021 ◽  
Author(s):  
Wenjie Lu ◽  
Nan Fu ◽  
Meng Wai Woo ◽  
Xiao Dong Chen
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Whey Protein ◽  
Spray Drying ◽  
Lactobacillus Rhamnosus ◽  
Protective Layer ◽  
Protein Isolate ◽  
Lactobacillus Rhamnosus Gg ◽  
Bacterial Viability ◽  
Protective Agents

Protective agents in spray drying protect the activity of lactic acid bacteria (LAB) by stabilizing subcellular structures, constituting a protective layer at cellular surface, or having mild drying kinetics. The...

SiO2 Entrapment of Animal Cells for Hybrid Bioartificial Organs

2000 ◽  
Vol 628 ◽  
Author(s):  
Giovanni Carturan ◽  
Renzo Dal Monte ◽  
Maurizio Muraca
Keyword(s):  
Gas Phase ◽  
Mechanical Stability ◽  
Porous Silica ◽  
Collagen Matrix ◽  
Homogeneous Layer ◽  
Animal Cells ◽  
Bioartificial Organs ◽  
Liver And Pancreas ◽  
Encapsulation Method ◽  
Metabolic Activities

ABSTRACTSi-alkoxides in gas phase are reactive towards the surface of animal cells, depositing a homogeneous layer of porous silica. This encapsulation method preserves cell viability and does not alter the hindrance of the biological load.In the prospective use for the design of a hybrid bioartificial liver, hepatocytes in a collagen matrix can be entrapped by the siliceous deposit which provides definite mechanical stability to the collagen matrix and molecular cutoff vs. high molecular weight proteins, including immunoglobulins. The functionality of the encapsulated cell load is maintained for the expressions of typical liver and pancreas metabolic activities.

The effect of probiotics on the level of immunoglobulin A in women with violation of the biocenosis of the vagina

2017 ◽  
pp. 85-88
Author(s):  
O.I. Ostapenko ◽  
◽  
V.P. Kvashenko ◽  
I.K. Akimova ◽  
I.N. Nosova ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Lactobacillus Rhamnosus ◽  
Test System ◽  
Main Group ◽  
Reproductive Age ◽  
Local Action ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Local Immunity ◽  
Serum Iga

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina. Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment. A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets. Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal. Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina. Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

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