Competitive displacement of interfacial soy proteins by Tween 20 and its effect on the physical stability of emulsions

2021 ◽  
Vol 113 ◽  
pp. 106515
Author(s):  
Shao Bing Zhang ◽  
Dong Qiong Yan ◽  
Yu Shan Jiang ◽  
Chang He Ding
Keyword(s):  
Physical Stability ◽  
Soy Proteins ◽  
Tween 20 ◽  
Competitive Displacement

scholarly journals Inter-Correlation among the Hydrophilic–Lipophilic Balance, Surfactant System, Viscosity, Particle Size, and Stability of Candelilla Wax-Based Dispersions

Coatings ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 469 ◽  
Author(s):  
Martina Lindner ◽  
Magdalena Bäumler ◽  
Andreas Stäbler
Keyword(s):  
Particle Size ◽  
Emulsion Stability ◽  
Physical Stability ◽  
Stability Index ◽  
Continuous Phase ◽  
Tween 20 ◽  
Flotation Rate ◽  
Hydrophilic Lipophilic Balance ◽  
Emulsion Stability Index ◽  
Candelilla Wax

Owing to a decrease in mineral oil resources, it is crucial to develop packaging materials based on renewable resources. Hence, a water vapor-barrier coating is developed as a natural wax-based dispersion. This dispersion should be stable over the storage time. In this study, the physical stability of a wax-based melt dispersion was analyzed (24 h and 21 days after production), and instability phenomena such as agglomeration, coalescence, and flotation were identified. Furthermore, the inter-correlations among the particle size, viscosity of the continuous phase, physical stability, surfactant chemistry, and hydrophilic–lipophilic balance value were characterized. Particle sizes were described by volume/surface mean d3,2, volume moment mean d4,3, and number mean d1,0 diameter, as well as the span of the volume and number distribution. Stability was characterized by the flotation rate, emulsion stability index, and Turbiscan stability index. Coalescence and agglomeration were not observed after the solidification of the wax particles. A significant correlation was observed for the emulsion stability index, with d3,2, and for flotation rate, with d1,0, d4,3, and viscosity as well, with d1,0, d3,2. Surfactants with hydrophilic–lipophilic balance values of 11–13.5 seem to be the most suitable for stabilizing candelilla wax-in-water suspensions. Particles were smaller, and wax suspensions were better stabilized using Tween 20 and Span 20, compared with Tween 80 and Span 80.

Influence of Composition of Emulsifier Blends on the Rheological Properties of Salad Dressing-Type Emulsions

2003 ◽  
Vol 9 (1) ◽  
pp. 53-63 ◽  
Author(s):  
M. A. Riscardo ◽  
J. M. Franco ◽  
C. Gallegos
Keyword(s):  
Rheological Properties ◽  
Size Distribution ◽  
Droplet Size ◽  
Physical Stability ◽  
Droplet Size Distribution ◽  
Egg Yolk ◽  
Tween 20 ◽  
Binary Blends ◽  
Sorbitan Monolaurate ◽  
Salad Dressing

This paper deals with the influence that composition of emulsifier blends exerts on the rheological properties of low-in-fat salad dressing-type emulsions. Binary blends of egg yolk and different types of amphiphilic molecules (low-molecular weight and macromolecules) were used in several proportions to stabilize emulsions by keeping constant the total amount of emulsifier. The different emulsifiers added to egg yolk were pea protein, sodium caseinate, polyoxyethylene(20)-sorbitan monolaurate (Tween 20) and sucrose distearate. Steady state flow tests and small-amplitude oscillatory measurements within the linear viscoelasticity region were carried out. Rheological tests were complemented with droplet size distribution measurements and observation of physical stability against creaming of these emulsions. It was pointed out that rheological properties, droplet size and physical stability of the emulsions studied depended on the weight ratio of emulsifiers in the binary blends, although the emulsifier total concentration remained constant, as well as the nature of the substance blended with egg yolk. These results have been explained on the basis of the relationship among rheological properties, droplet size distribution, continuous phase characteristics and interactions among different emulsifier molecules.

Proniosomal Formulation Encapsulating Pomegranate Peel Extract for Nutraceutical Applications

2021 ◽  
Vol 21 (5) ◽  
pp. 2907-2916
Author(s):  
Seyedeh Shaghayegh Alavi ◽  
Seyed Hadi Razavi ◽  
Faramarz Khodaiyan ◽  
Maria Cristina Cardia ◽  
Francesco Lai ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Cytotoxicity Assay ◽  
Tween 20 ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Traditional Natural ◽  
Span 60 ◽  
Pomegranate Peel Extract

In this study, pomegranate peel as a traditional natural remedy was extracted and encapsulated in proniosomal systems in order to improve its stability against harsh environmental conditions. Pomegranate peel was extracted by using sonication as a green extraction technology and the antioxidant activity of the obtained extract was evaluated to be 85.37% by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Proniosomal powder was prepared based on the slurry method with a mixture of non-ionic surfactants namely span 60 and tween 20 in combination with cholesterol as a bilayer stabilizer. Proniosome-derived niosomes were achieved by hydration of the powder with water. The obtained vesicles were evaluated for their particle size, morphological observations, entrapment efficiency, cytotoxicity assay, DPPH antioxidant activity and, physical stability at 4 °C for 28 days. The results demonstrated that the proniosome-derived niosomes were of small size (198.16 nm for unloaded and 411.3 for extract loaded), quite homogeneous (PDI = 0.188 for unloaded and 0.216 for loaded) with highly negative charged spherical vesicles and showed appropriate physical stability during the time of storage. The encapsulation efficiency was 68.43±0.24% and the cytotoxicity assay proved that the formulations were not toxic against 3T3 fibroblast cells in the applied concentration.

scholarly journals Comparative Study of Physicochemical Properties of Nanoemulsions Fabricated with Natural and Synthetic Surfactants

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2002
Author(s):  
Karen Fuentes ◽  
Claudia Matamala ◽  
Nayaret Martínez ◽  
Rommy N. Zúñiga ◽  
Elizabeth Troncoso
Keyword(s):  
Physicochemical Properties ◽  
Droplet Size ◽  
Physical Stability ◽  
Droplet Size Distribution ◽  
Protein Isolate ◽  
Tween 20 ◽  
Synthetic Surfactant ◽  
Soy Lecithin ◽  
Food Grade ◽  
Droplet Size Distributions

This work aims to evaluate the effect of two natural (whey protein isolate, WPI, and soy lecithin) and a synthetic (Tween 20) emulsifier on physicochemical properties and physical stability of food grade nanoemulsions. Emulsions stabilized by these three surfactants and different sunflower oil contents (30% and 50% w/w), as the dispersed phase, were fabricated at two levels of homogenization pressure (500 and 1000 bar). Nanoemulsions were characterized for droplet size distribution, Zeta-potential, rheological properties, and physical stability. Dynamic light scattering showed that droplet size distributions and D50 values were strongly affected by the surfactant used and the oil content. WPI gave similar droplet diameters to Tween 20 and soy lecithin gave the larger diameters. The rheology of emulsions presented a Newtonian behavior, except for WPI-stabilized emulsions at 50% of oil, presenting a shear-thinning behavior. The physical stability of the emulsions depended on the surfactant used, with increasing order of stability as follows: soy lecithin < Tween 20 < WPI. From our results, we conclude that WPI is an effective natural replacement of synthetic surfactant (Tween 20) for the fabrication of food-grade nanoemulsions.

An immunocytochemical study of maternal immunoglobulin localization in the suckling pig intestine

1990 ◽  
Vol 48 (3) ◽  
pp. 922-923
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath
Keyword(s):  
Sodium Ethoxide ◽  
Intracellular Localization ◽  
Protein A ◽  
Tween 20 ◽  
Intestinal Epithelial ◽  
Immunocytochemical Study ◽  
Fish Skin ◽  
Newborn Piglets ◽  
Rabbit Igg ◽  
Fish Skin Gelatin

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.

DEVELOPMENT OF OVARIAN AUTOGRAFTS ON THE GREATER OMENTUM IN SPAYED HYPOPHYSECTOMIZED RATS AND IN CASTRATES TREATED WITH METHALLIBURE, GONADOTROPHINS AND OESTRADIOL BENZOATE

1972 ◽  
Vol 70 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Artur Ber
Keyword(s):  
Body Weight ◽  
Greater Omentum ◽  
Tween 20 ◽  
Control Groups ◽  
Complete Cessation ◽  
Oestradiol Benzoate ◽  
Hypophysectomized Rats ◽  
Growth Promoting

ABSTRACT Hypophysectomy was performed at the same time as ovariectomy in 19 rats aged 6 weeks; in addition a fragment of the animal's own ovary was implanted into the greater omentum. The graft took but there was complete cessation of the growth of the implant and a decrease in body and uterine weights as compared to 15 ovariectomized controls of the same age killed after one month. This shows that the pituitary is indispensable for the growth of ovarian implants but not for their take. In 84 spayed rats aged 3 weeks with ovarian implants, methallibure (6 mg/day in a 0.2% solution of Tween 20) was given by gavage during one month. Some of them were treated with PMSG (20 IU/day), HCG (20 IU/day) or with oestradiol benzoate (0.05 mg/day) only or in combinations. As controls, there were 74 spayed rats with ovarian implants, seven of which received Tween 20, and 67 were untreated. No differences were found between the two control groups. Methallibure alone caused arrest of the development of the implants and uteri and a decrease in body weight. In the methallibure treated animals HCG stimulated the growth of the implant which was, however, smaller than that in the controls, while PMSG restored the weight of the implant even above that of the control rats. Oestradiol benzoate caused a further diminution in the size of the implants but augmented considerably the influence of HCG on the implants. It had no effect on the weight of the implant in rats treated with PMSG. It is concluded that FSH is the principal implant growth-promoting factor and that oestrogens appear to act in two directions. They decrease the gonadotrophin output from the pituitary thus inhibiting the growth of the implants, but in the presence of gonadotrophins they act directly on the implants, promoting their development.

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